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Human Papillomavirus (HPV) infection is typically the cause of cervical cancer, one of the cancers with a high death rate among women. Despite their widespread use, conventional therapeutic approaches still have drawbacks, such as the emergence of cancer cell resistance and severe side effects. Natural compounds are now used as safer alternative treatments as a result. Soursop leaves (Annona muricata L.) are one plant that has been shown to have anticancer potential. Through phytochemical screening, compound identification using GC-MS, cell viability testing using the MTT Assay, and BCL2 gene expression analysis using qPCR, this study attempts to examine the potential of soursop leaf extract as an anticancer agent against cervical cancer cells (HeLa cells). The results of the phytochemical examination showed that steroids, phenolics, and alkaloids were present. Ostole, bisacumol, and ricinoleic acid were found to be the predominant components when the active chemicals were identified by GC-MS. With an IC²₀ value of 5.01 μg/mL, the MTT experiment demonstrated that soursop leaf extract could decrease cell viability as the concentration rose. Furthermore, BCL2 gene expression was significantly reduced, especially in treatments with doses of 500 and 1000 μg/mL, according to the results of qPCR gene expression analysis. The overall findings suggest that soursop leaf extract may be used as a natural anticancer treatment against HeLa cells by lowering the expression of the BCL2 gene.

Background: Acne vulgaris is one of the most common skin problems and is closely associated with the activity of Propionibacterium acnes. Long-term use of antibiotics may lead to bacterial resistance; therefore, alternative antibacterial agents derived from natural products are needed. White rice (Oryza sativa L.) is known to contain bioactive compounds such as flavonoids, alkaloids, terpenoids, tannins, and saponins, which potentially exhibit antibacterial activity. Objective: This study aimed to evaluate the antibacterial activity of ethanol extract of white rice against the growth of Propionibacterium acnes. Methods: This research was conducted as a laboratory experimental study using a post-test only control group design. The ethanol extract of white rice was prepared by maceration using 70% ethanol. Antibacterial activity was evaluated using the disc diffusion method with extract concentrations of 100%, 50%, 25%, 12.5%, and 6.25%. Clindamycin was used as a positive control, while sterile distilled water served as a negative control. The parameter observed was the diameter of the inhibition zone. Result: The results of phytochemical screening showed that the ethanol extract of white rice (Oryza sativa L.) contains flavonoids, alkaloids, and terpenoids. Antibacterial activity test showed that the ethanol extract of white rice at concentrations of 100%, 50%, and 25% was able to inhibit the growth of Propionibacterium acnes by forming an inhibition zone, while at concentrations of 12.5% and 6.25% no inhibition zone was formed. The highest inhibition zone diameter was found at a concentration of 100%. The ethanol extract of white rice at concentrations of 100% (7.65 mm), 50% (6.77 mm), and 25% (6.15 mm) was able to inhibit the growth of Propionibacterium acnes, and was classified as having moderate inhibition. In contrast, at concentrations of 12.5% and 6.25%, the ethanol extract of white rice did not show any inhibitory activity detected in vitro using the disc diffusion method. The results of statistical analysis showed a p value <0.001, which means there was a significant difference in the diameter of the inhibition zone between the treatment groups. Conclusion: In conclusion, the ethanol extract of white rice exhibits antibacterial activity against Propionibacterium acnes in a concentration-dependent manner; however, its inhibitory effect remains lower than that of clindamycin.

This research aims to determine the effect of ethanol extract of bidara leaves on the degradation of S. aureus biofilms. This research is an experimental study with 7 treatment groups. KN (negative control given distilled water), KP (positive control, given Erythromycin), ethanol extract concentration treatment groups namely P1 60%, P2 70%, P3 80%, P4 90%, P5 100%. The research design used was 28 samples calculated using the Federer formula. The biofilm degradation test was carried out using the crystal violet binding assay technique. Data were analyzed using the Kruskal Wallis test with a p value of 0,005 (<0,05) which was significantly different and continued with the Mann-Whitney Post Hoc test. Biofilm degradation test results of bidara leaf ethanol extract at concentrations of 60%, 70%, 80%, 90% and 100% were able to degrade S. aureus bacterial biofilms up to 19,32%, 5,42%, 10,72%, 24,16% and 38,80%, in the positive control groups (erythromycin) it was 94,84% and in the negative control (distilled water) 0,00%. It can be concluded that the ethanol extract of bidara leaves (Z. mauritiana Lamm) is able to degrade S. aureus bacterial biofilms up to 38,80%.

Background: Diabetes Mellitus (DM) is a disease that cannot be completely cured or cannot even be completely cured. The vile shard plant is empirically used by the community to treat diabetes (DM). This study aims to conduct phytochemical screening and test the activity of 96% ethanol extract of kejibeling leaves (Strobilanthes crispus (L.) Bl.) as a herbal antidiabetic in male white mice (Mus musculus) with alloxan induction. Method: This research is an experimental laboratory research with a true experimental posttest control design using a completely randomized design (CRD) with 5 treatments and 5 replications. Treatment P1 (without treatment) as normal control (N), P2 as positive control (+), P3 as negative control (-), P4 kejibeling leaf extract 250 mg/kg BW, P5 kejibeling leaf extract 500 mg/kg BW. Result:The results of phytochemical screening showed the presence of alkaloids, flavonoids, tannins, saponins, terpenoids and steroids. SPSS results show that the data is normally distributed (p>0.05) and homogeneous (p>0.05). The results of the ANOVA on the treatment of giving keji beling leaf extract 250 mg/Kg BW showed a sig. 0.393 (p>0.05) and treatment of 500 mg/Kg BW obtained a sig value. 0.517 (p>0.05). Conclusion:The conclusion from the research results shows that administering doses of 250 mg/kg BW and 500 mg/kg BW of keji beling leaf extract can reduce blood sugar levels in mice. It is hoped that the results of this research will be useful for the community as an antidiabetic therapy using kejibeling leaves (Strobilanthes crispus (L.) Bl.).

Indonesia, as a tropical country, has high ultraviolet (UV) radiation intensity, which increases the risk of skin damage due to oxidative stress. This study aimed to determine the correlation between Sun Protection Factor (SPF) values and antioxidant capacity of the ethyl acetate fraction from bandotan leaves (Ageratum conyzoides L.). The study involved extraction by maceration using 96% ethanol, liquid-liquid fractionation, phytochemical screening, antioxidant activity testing using the ABTS method, and SPF value determination by UV-Vis spectrophotometry. The results showed that the ethyl acetate fraction exhibited the highest antioxidant activity with an IC₅₀ value of 21.726 ppm (very strong category) and the highest SPF value of 23.28 (ultra protection category). Statistical analysis indicated a significant positive correlation between antioxidant capacity and SPF values, with a correlation coefficient (R) of 0.647 and a coefficient of determination (R²) of 0.418 (p < 0.05). This study demonstrates that the ethyl acetate fraction of bandotan leaves has potential to be developed as a natural sunscreen ingredient with high antioxidant activity.

Bitter melon (Momordica charantia) is a medicinal plant traditionally used to control blood glucose levels and improve digestive health. These benefits are closely associated with its bioactive metabolite content. This article aims to characterize the bioactive metabolites of bitter melon and to review their potential bioactivity in glycemic control and digestive function. The method employed includes a literature-based characterization of metabolites, identification of major classes of active compounds, and evaluation of analytical techniques commonly applied in natural product research, such as phytochemical screening, chromatography, and spectroscopic analysis. The results indicate that bitter melon contains various bioactive metabolites, including flavonoids, alkaloids, saponins, triterpenoids, and polyphenols, which contribute to blood glucose reduction by enhancing insulin sensitivity and inhibiting glucose absorption. Furthermore, these compounds support digestive health by improving digestive enzyme activity, exhibiting anti-inflammatory effects, and maintaining gut microbial balance. In conclusion, bitter melon represents a promising source of natural bioactive compounds with significant potential for application in health and pharmaceutical development.

Lemongrass (Cymbopogon citratus) is a plant known to contain various bioactive compounds with potential antioxidant properties as well as xanthine oxidase (XO) inhibitory activity. This study aimed to evaluate the phytochemical content, antioxidant activity, and XO inhibitory potential of ethanol extracts from the leaves, stems, and their combination. Phytochemical screening revealed that all extracts contained alkaloids, flavonoids, and terpenoids, while phenolic compounds were detected only in the leaf extract and the combined leaf–stem extract. Antioxidant activity assays demonstrated that all extracts exhibited very strong antioxidant activity, with IC₅₀ values below 50 ppm, indicating a significant potential to scavenge free radicals. In the XO inhibition assay at a concentration of 200 ppm, the ethanol extract of lemongrass stems showed the highest inhibitory activity at 81.37%, followed by the leaf extract at 48.08% and the combined leaf–stem extract at 33.65%. Overall, these findings suggest that the ethanol extract of lemongrass stems is the most promising natural source of antioxidants and has the greatest ability to inhibit xanthine oxidase activity, indicating its potential development as a functional ingredient for health applications.

Pacing tawar is one of many plants used in alternative medicine. Pacing tawar is often used as an ingredient in herbal remedies for various ailments. Pacing leaves are effective as a remedy for itching, insect bites, and hair growth. This study aims to identify the presence and class of secondary metabolites in the ethanol extract of pacing tawar leaves to find bioactive compounds that have the potential to have pharmacological and therapeutic activities. Extraction was carried out by the maceration method using 96% ethanol solvent until a thick extract was obtained. Next, phytochemical screening was carried out to identify the content of alkaloids, flavonoids, saponins, tannins, and steroids. The test results showed that the extract of pacing tawar leaves was positive for alkaloids, saponins, tannins, and steroids, and negative for flavonoids. The alkaloid test showed a dark pink color change, the saponin test produced a 2 cm high foam for 30 seconds, the tannin test produced a dark green color, and the steroid test showed a faint red ring. Based on these results, it can be concluded that freshwater pacing leaves contain various bioactive compounds that have the potential to be developed as natural medicinal ingredients.

The wild ginger leaves (Costus speciosus) is used by the community as a traditional medicine, with its rhizome being utilized as an antipyretic, diuretic and anti-inflammatory agent. This plant contains secondary metabolite compounds such as alkaloids, flavonoids, polyphenols, quinones, tannins and saponins. This research aims to identify saponin compounds and characterize the moisture content and ash content in the ethanol extract of the white costus (Costus speciosus) leaves. The white costus leaves sample was extracted using the maceration method with 96% ethanol as the solvent. The extraction yielded a rendemen value of 13.3%. Non-specific characterization tests included moisture content and ash content. The results for the moisture content of the white costus leaf simplicia were 3.3%, which meets the quality requirement of not more than 10% according to Regulation of the Food and Drug Supervisory Agency Number 32 of 2019. The ash content test result for the simplicia was 0.97%, which also meets the standard requirement of the Indonesian Herbal Pharmacopoeia, which is less than 10.7%. Phytochemical screening was conducted to identify the types of compounds contained in the extract. Based on the phytochemical screening results, the 96% ethanol extract of white costus leaves positively contains alkaloids, tannins, saponins, and steroids.

This paper presents a narrative review focusing on the significance of in silico methodologies in identifying and evaluating natural bioactive compounds as promising antidiabetic agents. By examining scientific publications from 2020 to 2025, the review highlights substantial evidence that various plant-derived metabolites such as flavonoids, phenolic acids, terpenoids, and glycosides from Orthosiphon stamineus, Moringa oleifera, Syzygium polyanthum, Tinospora crispa, and Albizia saman demonstrate notable affinity toward several crucial enzymes implicated in type 2 diabetes, including DPP-4, SGLT-2, α-glucosidase, and PTP1B. Findings from molecular docking analyses and ADMET simulations indicate that many of these compounds exhibit favorable pharmacokinetic behavior, strong receptor interactions, and minimal toxicity, suggesting their potential as multitarget natural therapeutics with synergistic modes of action. Utilizing in silico screening as an initial step effectively predicts biological activity and pharmacological profiles prior to experimental validation through in vitro and in vivo testing, thus reducing both time and research expenditure. The synergy between computational modeling, medicinal chemistry, and phytochemical exploration establishes a scientific framework that supports the advancement of rational, safe, and sustainable herbal-based antidiabetic drug development derived from Indonesia’s vast biodiversity and encourages self-reliance within the national pharmaceutical sector.

Citrus hystrix DC., commonly known as kaffir lime, is a citrus plant widely distributed in Southeast Asia including Indonesia, Thailand, India, and China. Its leaves are traditionally used as food flavoring and herbal medicine due to their distinctive aroma and therapeutic potential. This study was conducted to identify the presence of secondary metabolite compounds in kaffir lime leaves through qualitative phytochemical screening. Fresh leaves were extracted and tested using standard phytochemical methods with specific reagents to detect alkaloids, flavonoids, phenolics, tannins, saponins, steroids, and terpenoids. The screening process was carried out through colorimetric reactions that indicate the presence of specific metabolite groups. The results showed that kaffir lime leaves positively contain several classes of secondary metabolites, including alkaloids (brownish-black precipitate with Bouchardat reagent), flavonoids (red color with Mg and concentrated HCl), saponins (stable foam formation after shaking with HCl), steroids (green color with acetic anhydride), terpenoids (reddish-purple color with concentrated H2SO4), and tannins (greenish-black color with FeCl3). The findings confirm that kaffir lime leaves are rich in bioactive compounds that may contribute to pharmacological activities such as antimicrobial, antioxidant, anti-inflammatory, and therapeutic properties. These results support the traditional use of kaffir lime leaves as natural medicine and highlight their potential as a source of natural bioactive compounds for further pharmaceutical and nutraceutical development. This study provides preliminary scientific evidence and encourages more detailed quantitative and isolation studies on the active components of Citrus hystrix.

Fungal infections are a common health problem in tropical regions, including Indonesia, since humid conditions support microbial growth. Candida tropicalis is a pathogenic species frequently identified as the cause of both superficial and systemic candidiasis. The rising resistance to synthetic antifungal agents such as fluconazole and ketoconazole highlights the need for safer and more effective alternative therapies. Propolis, a resinous substance produced by Trigona sp. bees from various plant sources, contains flavonoids, phenolics, and terpenoids with important biological activities, including antimicrobial and antifungal effects. This study aimed to evaluate the effectiveness of ethanol extract of propolis from Balikpapan against the growth of Candida tropicalis using the disk diffusion method. Phytochemical screening revealed the presence of alkaloids, flavonoids, phenolics, tannins, and terpenoids in the extract. Antifungal activity testing showed that concentrations of 80%, 90%, and 100% significantly inhibited fungal growth, with the largest inhibition zone of 27.93 mm observed at 100% concentration. Statistical analysis using ANOVA confirmed significant differences among treatments. Thus, ethanol extract of Trigona sp. propolis from Balikpapan demonstrates strong potential as a natural antifungal agent. These findings support the use of propolis as a natural-based therapeutic alternative for fungal infections and contribute to the development of herbal pharmaceutical products.

Leaves of Etlingera walang (Blume) RMSm have been empirically used in traditional medicine to relieve stomach disorders and are known to contain secondary metabolites with potential antimicrobial properties. Salmonella typhi infection can cause typhoid fever with symptoms such as diarrhea and abdominal pain, while Candida tropicalis is a fungus that can infect the gastrointestinal tract and lead to digestive disturbances. This study aimed to evaluate the antibacterial and antifungal activities of the ethanolic extract of E. walang leaves against S. typhi and C. tropicalis. The antimicrobial assay was carried out using the disk diffusion method at extract concentrations of 25%, 50%, and 100%, with chloramphenicol and ketoconazole as positive controls, and DMSO as the negative control. Phytochemical screening revealed the presence of alkaloids, flavonoids, saponins, and tannins. However, the results showed that the extract did not produce inhibition zones against either test microorganism. Extract evaluation indicated a moisture content of 12.23%, total ash 13.39% (above the standard), acid-insoluble ash 1.25%, total plate count 1.8 × 10 ⁴ , and mold and yeast count 1.0 × 10 ³. The findings suggest that although the ethanolic extract of E. walang leaves contains secondary metabolites and is traditionally used for stomach ailments, it does not exhibit antimicrobial activity against S. typhi or C. tropicalis.

Butterfly pea (Clitoria ternatea L.) is a type of ornamental plant characterized by flowers in shades of purple, blue, pink, and white. Besides being an ornamental plant, butterfly pea is also commonly used in traditional medicine. Several previous studies have confirmed its health benefits. Therefore, it is necessary to conduct phytochemical screening for flavonoids, saponins, and alkaloids using the Wilstater method, foam test, Dragendorff’s reagent, and Wagner's test. This study demonstrates the presence of flavonoid, saponin, and alkaloid compounds in butterfly pea flowers, indicated by positive results in various tests conducted.

This study investigated the phytochemical screening and antibacterial activity against various microorganisms including E. coli, Staphylococcus aureus, Klebsiella pneumoniae, Enterococcus faecalis, and Candida albicans. Active compounds were isolated and identified from Aleppo Oak Gallnut, obtained from the Iraqi plant Quercus Infectoria L. Plant extracts were prepared using a continuous extraction apparatus, Soxhlet, with a successive solvent system based on polarity differences, including petroleum ether (60-80°C), ethanol (78°C), and hot aqueous extracts. Acid hydrolysis was performed on the raw ethanol and hot water extracts to obtain free phenolic compounds, including Gallic acid, Apigenin, Rutin, Kaempferol, Chlorogenic acid, and Caffeic acid, using high-performance liquid chromatographic (HPLC) techniques. The inhibitory activity of Aleppo Oak Gallnut extracts (Ethanol and Hot Aqueous) after acid hydrolysis was tested using four concentrations (25%, 50%, 75%, 100%) against various microorganisms. The Ethanol extract exhibited a significant inhibitory effect on Enterococcus faecalis. The Hot Aqueous extract showed a modest inhibitory effect on Enterococcus faecalis. The Ethanol extract demonstrated strong inhibition against Klebsiella pneumoniae. The Hot Aqueous extract at 100% and 75% showed high inhibition. The Ethanol extract exhibited significant inhibitory effects against Staphylococcus aureus at 100%. The Hot Aqueous extract at 75% and 100%  demonstrated weaker inhibition against Staphylococcus aureus. The Ethanol extract demonstrated weaker inhibition against E. coli, and the Hot Aqueous extract showed no effect at 50%  and 25%, and slight inhibition at 100%  and 75%. For Candida albicans, the Ethanol extract showed minimal inhibition at all concentrations and no effect at 25%. The Aqueous extract had a slight effect at 100%  and no inhibition at 75%, 50%, and 25%.

Indonesia's biodiversity holds great potential for drug development, including through the use of traditional medicinal plants such as tapak liman (Elephantopus scaber L.), suruhan (Peperomia pellucida L. Kunth), and temu ireng (Curcuma aeruginosa Roxb.). These plants have been used in traditional medicine and are known to contain bioactive compounds with potential anticancer properties. Tapak liman contains compounds capable of inducing apoptosis in cancer cells. Suruhan is known to have cytotoxic and immunomodulatory effects, while temu ireng contains compounds with antioxidant and anticancer activity. This study aims to identify and quantify the bioactive compounds in these three plants using Gas Chromatography-Mass Spectrometry (GC-MS) and to explore their therapeutic potential in cancer treatment. The results of GC-MS analysis successfully identified six main compounds that have potential as anticancer agents, namely Phytol, Caryophyllene, Apiol, Germacrone, Germacrene B, and one additional compound. Temu ireng contains Germacrone, Germacrene B, and Caryophyllene, while suruhan contains Caryophyllene, Apiol, and Phytol. Meanwhile, tapak liman shows the presence of bioactive compounds that support cytotoxic activity. The focus of this study is on the interaction of bioactive compounds with the Bcl-2 protein, which plays a role in inhibiting apoptosis in cancer cells. Additionally, molecular analysis revealed that Germacrone, Germacrene B, Caryophyllene, and Apiol can also bind to the p53 protein, known as a guardian of the genome and a trigger for apoptosis. These findings suggest that the three plants have high potential as natural sources of anticancer agents. Further in vitro and in vivo studies are recommended to test the biological efficacy of these compounds in the context of cancer therapy, thereby bridging traditional knowledge with modern scientific approaches in the development of natural-based medications.

Malaria remains a significant public health issue in Indonesia, particularly in endemic areas such as Keerom Regency, Papua. Efforts to control this disease face challenges due to Plasmodium resistance to synthetic drugs, highlighting the need for safer and more effective alternative treatments. This study aims to identify the secondary metabolite content of several local plants traditionally used as antimalarial remedies. An experimental laboratory study was conducted using phytochemical screening methods. The plants examined included turmeric rhizome (Curcuma longa), meniran leaves (Phyllanthus niruri), papaya leaves (Carica papaya), sambiloto (Andrographis paniculata), and brotowali (Tinospora crispa). Extraction was carried out using the maceration method with 96% ethanol as the solvent. The results showed that all samples contained secondary metabolites such as flavonoids, alkaloids, tannins, and saponins, which are known for their antiplasmodial activity. Extract yields ranged from 18% to 24%, with turmeric rhizome showing the highest yield. These findings suggest that local plants from Keerom Regency hold potential as raw materials for the development of herbal antimalarial drugs. This study serves as a preliminary step for further in vitro and in vivo bioactivity testing.

This study aimed to identify the secondary metabolite compounds present in the ethanol extract of Plukenetia volubilis L. (sacha inchi) shells through phytochemical screening and LC-MS analysis. The extraction was performed using the maceration method with 95% ethanol as solvent, resulting in a yield of 47.33%. Qualitative phytochemical tests revealed the presence of alkaloids, flavonoids, tannins, terpenoids, saponins, and glycosides in the extract. Further LC-MS analysis tentatively identified several phenolic and flavonoid compounds with known biological activities, including sinapinic acid, 1-o-sinapoylglucose, and azelaic acid from the dicarboxylic acid group. The presence of these compounds highlights the potential of sacha inchi shell extract as a natural antioxidant and antidiabetic agent. These findings support the valorization of sacha inchi agricultural waste as a promising raw material for pharmaceutical and nutraceutical applications.

Breadfruit (Artocarpus heterophyllus) leaves are widely used in traditional medicine in Southeast Asia. Phytochemical screening of breadfruit leaves showed that the plant is rich in chemical contents that have potential as a source of natural medicine. The biological activity of breadfruit leaf phytochemicals showed potential in the treatment of various diseases. The benefits of phytochemicals (Development of natural medicines, Discovery of new chemical compounds, Development of cosmetic and food products, Improvement of quality of life through the use of natural products and Conservation of rare and endemic plants). this research uses the method Phytochemical screening of breadfruit leaves involves several stages: Extraction, Fractionation, Identification. The results stated that breadfruit leaves contain Steroid / Terpenoid compounds which indicate positive results in concentrated green, Saponin negative results that only precipitate not, Tannin positive results in blackish color, Flaphonoid positive results in red color and Alkoloid positive results in brown color.  

Phytochemistry is a method used to determine the compounds found in plant essence. Phytochemical identification is carried out using chemical substances used in a reaction to determine the collection of secondary metabolite compounds. Indonesia is a country that has various types of medicinal plants, one of which is the leaves of Katang-katang (Ipomoea pes-caprae). Katang-katang is a tropical vine that is easy to find and belongs to the Convolvulaceae family. The utilization of this medicinal plant is one of the alternatives in an effort to treat a disease.  Along the road in the sandy beach area, many of these plants are found growing creeping on the edge of the shoreline, this plant has a trumpet-like flower and is known as Katang-katang (Ipomoea pes-caprae). (Ipomoea pescaprae L.). Empirically, people often use katang-katang plants to treat infections in wounds, relieve pain, and as antioxidants. The active compounds of katang-katang as antibacterial are terpenoids, steroids, saponins, tannins and flavonoids. The active compound that plays the most role is thought to be the flavonoid compound content.