Eksplorasi Senyawa dan Efek Ekstrak Daun Sirsak (Annona muricata L.) terhadap Viabilitas dan Ekspresi Gen BCL2 pada Sel HeLa

Jasmin Ayla Fitri Daulay ; Hanifa Tasya Kamila ; Rahma Yulia ; Nadila Ananda Putri
Jurnal Sains dan Kesehatan (JUSIKA) 📅 2026 Vol 10, No 1 🔗 DOI: 10.57214/jusika.v10i1.1149
Abstract
Human Papillomavirus (HPV) infection is typically the cause of cervical cancer, one of the cancers with a high death rate among women. Despite their widespread use, conventional therapeutic approaches still have drawbacks, such as the emergence of cancer cell resistance and severe side effects. Natural compounds are now used as safer alternative treatments as a result. Soursop leaves (Annona muricata L.) are one plant that has been shown to have anticancer potential. Through phytochemical screening, compound identification using GC-MS, cell viability testing using the MTT Assay, and BCL2 gene expression analysis using qPCR, this study attempts to examine the potential of soursop leaf extract as an anticancer agent against cervical cancer cells (HeLa cells). The results of the phytochemical examination showed that steroids, phenolics, and alkaloids were present. Ostole, bisacumol, and ricinoleic acid were found to be the predominant components when the active chemicals were identified by GC-MS. With an IC²₀ value of 5.01 μg/mL, the MTT experiment demonstrated that soursop leaf extract could decrease cell viability as the concentration rose. Furthermore, BCL2 gene expression was significantly reduced, especially in treatments with doses of 500 and 1000 μg/mL, according to the results of qPCR gene expression analysis. The overall findings suggest that soursop leaf extract may be used as a natural anticancer treatment against HeLa cells by lowering the expression of the BCL2 gene.
Keywords
Annona muricata L; BCL2; MTT Assay; qPCR; Sel HeLa
How to Cite

Jasmin Ayla Fitri Daulay, et al. (2026). Eksplorasi Senyawa dan Efek Ekstrak Daun Sirsak (Annona muricata L.) terhadap Viabilitas dan Ekspresi Gen BCL2 pada Sel HeLa. Jurnal Sains dan Kesehatan (JUSIKA), 10(1). https://doi.org/10.57214/jusika.v10i1.1149

Jasmin Ayla Fitri Daulay; Hanifa Tasya Kamila; Rahma Yulia; Nadila Ananda Putri, "Eksplorasi Senyawa dan Efek Ekstrak Daun Sirsak (Annona muricata L.) terhadap Viabilitas dan Ekspresi Gen BCL2 pada Sel HeLa," Jurnal Sains dan Kesehatan (JUSIKA), vol. 10, no. 1, 2026.

Jasmin Ayla Fitri Daulay; Hanifa Tasya Kamila; Rahma Yulia; Nadila Ananda Putri. "Eksplorasi Senyawa dan Efek Ekstrak Daun Sirsak (Annona muricata L.) terhadap Viabilitas dan Ekspresi Gen BCL2 pada Sel HeLa." Jurnal Sains dan Kesehatan (JUSIKA), vol. 10, no. 1, 2026.

Jasmin Ayla Fitri Daulay; Hanifa Tasya Kamila; Rahma Yulia; Nadila Ananda Putri. "Eksplorasi Senyawa dan Efek Ekstrak Daun Sirsak (Annona muricata L.) terhadap Viabilitas dan Ekspresi Gen BCL2 pada Sel HeLa." Jurnal Sains dan Kesehatan (JUSIKA) 10, no. 1 (2026).

Jasmin Ayla Fitri Daulay, et al. (2026) 'Eksplorasi Senyawa dan Efek Ekstrak Daun Sirsak (Annona muricata L.) terhadap Viabilitas dan Ekspresi Gen BCL2 pada Sel HeLa', Jurnal Sains dan Kesehatan (JUSIKA), 10(1). doi: 10.57214/jusika.v10i1.1149.

Jasmin Ayla Fitri Daulay; Hanifa Tasya Kamila; Rahma Yulia; Nadila Ananda Putri. Eksplorasi Senyawa dan Efek Ekstrak Daun Sirsak (Annona muricata L.) terhadap Viabilitas dan Ekspresi Gen BCL2 pada Sel HeLa. Jurnal Sains dan Kesehatan (JUSIKA). 2026;10(1).

Artikel Terkait

Membran Berbasis Β-Trikalsium Fosfat Sebagai Membran Barier Pada Guided Bone Regeneration: Tinjauan Naratif

Maria Rini Wulan Dhari; Eddy Eddy; Rosalina Tjandrawinata; Tansza Permata Setiana Putri

Hubungan Pengetahuan, Persepsi, Sikap, dan Sarana-Prasarana dengan Perilaku Personal Hygiene Saat Menstruasi pada Santriwati di Pondok Pesantren Tahfidzil Qur'an Daarussa'adah Pesawaran Tahun 2026

Anggie Pebriani; Dhiny Easter Yanti; Christin Angelina Febriani

Gambaran Hasil CT Scan pada Pasien Diabetes Melitus dengan Stroke di RSKD Dadi Makassar Tahun 2024–2025

Muhammad Arif Taufik; Prema Hapsari Hidayati; Dian Fahmi Utami; Mochammad Erwin Rachman; Muh. Jabal Nur

Analisis Faktor yang Berhubungan dengan Kinerja Perawat di Instalasi Rawat Inap Rsud Ratu Zalecha Martapura

Aqiella Farida Salsabila; Rosihan Adhani; Iwan Aflanie; Syamsul Arifin; Nia Kania

Dampak Stigma terhadap Kesejahteraan Psikologis Keluarga yang Merawat Orang dengan Gangguan Jiwa di Kabupaten Pamekasan

Willi Holis; Ida Wahyuni; Ahmad Zaini Arif

Hubungan Pengetahuan Ibu terhadap Kejadian Karies pada Anak PAUD Qalby Play School Kab. Bogor

Faradillah Usman; Fidzah Nur Fajrina Murad; Wirda Aulia; Nurul Fatimah
Tren Sitasi Jurnal