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Abstract: The rapid diffusion of Generative Artificial Intelligence (AI) has intensified public debate regarding its benefits, risks, and societal implications. This study investigates public sentiment and thematic structures surrounding Generative AI by analyzing Twitter discourse as a representation of large-scale, real-time public perception. The research addresses two main problems: how public sentiment toward Generative AI is distributed and what dominant themes shape this perception. Accordingly, the objective is to map both emotional polarity and thematic narratives embedded in social media conversations. A computational mixed-methods approach was employed using a dataset of 12,470 tweets collected on 17 December 2024. Sentiment classification was conducted using a transformer-based DistilBERT model, while semantic representations were generated with Sentence-BERT. Topic modeling was performed using BERTopic, integrating HDBSCAN clustering and class-based TF-IDF to extract coherent and interpretable topics. Human-in-the-loop validation supported the interpretive robustness of topic labeling. The findings reveal that public sentiment toward Generative AI is predominantly positive (41.8%), particularly in relation to productivity enhancement, education, and creative applications. Neutral sentiment (31.4%) reflects informational discourse, while negative sentiment (26.8%) centers on ethical concerns, privacy risks, misinformation, and AI hallucinations. Seven dominant topics were identified, with clear topic–sentiment alignment showing optimism in utility-driven themes and skepticism in ethics- and risk-related discussions. In conclusion, public perception of Generative AI is dualistic—characterized by strong enthusiasm alongside persistent caution. These results provide empirical insights for AI governance, responsible innovation, and future research on socio-technical impacts of Generative AI. *    

Indonesia has extraordinary resources and potential in developing renewable energy sources (RES), but various obstacles must be overcome in implementing RES. The purpose of this study is to analyze the gap in the application of RES. This gap includes energy knowledge, community participation, battery waste management, service quality, regulation, and legal policy. This study uses a mixed-methods approach, by conducting a structured questionnaire in quantitative data collection, while qualitative data collection through special interviews, focused group discussions, and conducting policy regulation analysis. The results show that 62% of people do not understand RES, 28% are involved in project planning, and 74% are unaware of SOP (standard operating procedures) regarding battery waste recycling. The results of the correlation analysis reveal a positive relationship between the level of knowledge and interest in RES (R = 0.56). Also, the developed community-based participation model includes initial involvement, transparency of information, and local incentives. These findings further strengthen the compatibility of the innovation diffusion theory, planned behavior theory, SERVQUAL, and the theory of public interest. This study will make a practical contribution through evidence-based strategies in increasing resilience, especially for policymakers and energy service providers. The impact of the policy aspects includes the need for large reforms, education, public campaigns, and the realization of battery waste management systems. This study also provides an opportunity for further study by expanding the geographical scope and related industrial sectors.  

White Feces Disease (WFD), which infects Pacific white shrimp (Litopenaeus vannamei), poses a serious challenge in aquaculture due to its potential to cause mass mortality and substantial economic losses. One of the main causes of this disease is bacterial infection from the genus Vibrio. The use of natural substances, such as garlic (Allium sativum) extract, is considered a more environmentally friendly and sustainable alternative for disease control. This study aimed to examine the effect and determine the optimal concentration of garlic extract in inhibiting the growth of Vibrio sp. isolated from WFD-infected L. vannamei ponds, cultured on TCBS media. The study was conducted in vitro using the disc diffusion method with four extract concentrations (25%, 50%, 75%, and 100%) against two bacterial isolates, designated as isolate A and isolate B. The results showed that garlic extract was able to inhibit the growth of Vibrio sp., with the largest inhibition zone observed in isolate A at 75% concentration (14.8 mm) and in isolate B at 100% concentration (19.3 mm). These findings indicate the potential of garlic as a natural antibacterial agent to inhibit pathogenic bacteria and support the sustainability of shrimp aquaculture.

Pine soda is a natural fermented liquid from pine needles (Pinus merkusii) which has a distinctive aroma and contains bioactive compounds with potential as antimicrobial agents. This study aims to determine the antibacterial activity of pine soda against Staphylococcus aureus using the disc diffusion method and to compare its effectiveness with the antibiotic ampicillin as a positive control. The process of making pine soda is carried out through simple fermentation of Pinus merkusii pine needles under certain conditions to produce a fragrant liquid. Furthermore, qualitative tests are carried out to identify the content of bioactive compounds, such as flavonoids, phenols, and essential oils, which are known to have antibacterial properties. In the antibacterial activity test, paper discs that have been soaked in pine soda solutions with graded concentrations (2%, 4%, 6%, 8%, and 10%) are placed on agar media that have been inoculated with S. aureus. The results of the observation showed the presence of inhibition zones in each treatment with varying sizes. The largest inhibition zone was obtained at a concentration of 10% with a diameter of 10 mm, which is included in the medium inhibition category. As a comparison, ampicillin produced an inhibition zone of 26.3 mm, while distilled water, the negative control, showed no inhibition zone. These findings indicate that the antibacterial activity of pine soda is influenced by its bioactive compound content, although its effectiveness is still lower than that of standard antibiotics. This potential opens up opportunities for developing pine soda as a safe natural antibacterial agent, whether in the form of health products such as antiseptics, natural cleaners, or functional beverages that support body health. Further research is needed to optimize the fermentation process, increase the concentration of active compounds, and test its effectiveness against various types of pathogenic bacteria.

This study discusses the strategy of expanding the local Micro, Small, and Medium Enterprises (MSMEs) market through the development of Tourism Villages as a solution to limited market access and low competitiveness of MSME actors. MSMEs in Indonesia often face obstacles in aspects of the marketing mix such as venues, prices, products, and promotions. Through a qualitative approach, this study analyzes the direct impact of Tourism Villages on increasing market access, product innovation, and the role of innovation diffusion in encouraging technology adoption by MSME actors. The results of the study show that Tourism Villages such as Pujon Kidul, Taman Sari, and Sokobanah Daya are able to become a catalyst for the local economy by attracting tourists as potential consumers, reducing operational costs, and creating an innovation ecosystem that supports the growth of MSMEs. BUMDes (Village-Owned Enterprises) play an important role in digital promotion, product packaging development, and strengthening the capacity of local business actors through training and mentoring programs. Moreover, Tourism Villages encourage collaboration between stakeholders such as village governments, educational institutions, and private sectors in building sustainable local entrepreneurship. They also facilitate knowledge sharing and cultural preservation while integrating economic, social, and environmental values. Although there are still challenges such as lack of managerial skills, limited access to capital, and dependence on tourism seasons, the potential for long-term impact remains high. This study concludes that the integration of Tourism Village development with MSME strengthening strategies can be a model for inclusive and sustainable village economic empowerment. These findings provide practical recommendations for village governments, BUMDes managers, and MSME actors in building effective synergies that accelerate local economic growth and resilience.

Pedada leaves (Sonneratia caseolaris), a type of mangrove plant that grows in tropical and subtropical coastal areas, have high ecological and pharmacological value. In addition to functioning as coastal protection from abrasion and seawater intrusion, this plant is also known to contain various secondary metabolite compounds such as flavonoids, tannins, saponins, and alkaloids. These compounds have been widely reported to have important biological activities, one of which is as an antimicrobial agent. This study aims to identify the effectiveness of pedada leaf ethanol extract in inhibiting the growth of pathogenic microorganisms, especially bacteria that cause infections. The extraction process was carried out using 96% ethanol solvent through the maceration method, namely soaking the material in the solvent for a certain time to optimally obtain active compounds. The obtained extract was then tested for its antimicrobial activity using the disc diffusion method against test bacteria, both Gram-positive and Gram-negative. The inhibition zone formed around the disc was measured as an indicator of the antibacterial effectiveness of the extract. The results showed that pedada leaf extract was able to produce inhibition zones with varying diameters depending on the concentration used. The higher the extract concentration, the larger the diameter of the inhibition zone formed. This indicates a positive relationship between extract concentration and its antibacterial strength. This activity is believed to originate from the ability of compounds such as flavonoids and tannins to damage bacterial cell walls or disrupt microbial metabolic processes. This study provides initial evidence that pedada leaves have potential as an environmentally friendly and sustainable source of natural antibacterials. This potential is highly relevant in efforts to develop alternative antibacterial materials based on local plants, especially amidst increasing bacterial resistance to synthetic antibiotics.

Shrimp petis is a traditional Indonesian fermented product that is susceptible to contamination by pathogenic bacteria such as Staphylococcus aureus, Escherichia coli, and Pseudomonas spp. Preservation is generally carried out with chemical antibiotics, but their use can cause side effects and microbial resistance. This study aims to examine the effectiveness of ginger extract (Zingiber officinale) as a natural antibiotic against bacteria in shrimp petis. Ginger extract is known to contain active compounds such as gingerol and shogaol which have antibacterial activity. Through literature studies and disc diffusion method analysis, it was found that ginger extract was able to produce an inhibition zone of 12–22 mm against test bacteria. These results indicate that ginger extract is effective in inhibiting the growth of pathogenic bacteria and has the potential to be used as a natural preservative. In addition, a combination of biological, chemical, and natural preservation approaches is recommended to maintain the quality and safety of shrimp petis. The use of ginger extract is in line with the trend of healthy and environmentally friendly food.

Palm wine (tuak) is a traditional fermented beverage that is prone to microbial contamination, thus requiring natural alternatives with antibacterial properties. This study aimed to determine the effectiveness of Curcuma xanthorrhiza (temulawak) extract in inhibiting microbial growth in tuak samples. The method used was the disc diffusion test with three extract concentrations, 25%, 50%, and 100%. The tuak samples were first femented, serially diluted, and inoculated on Nutrient Agar (NA). Sterile paper discs soaked in each extract were placed on the medium surface and incubated at room temperature for 24 hours. The results showed no visible inhibition zones around the discs at any concentration tested. The conclusion of this study is that temulawak extract at concentrations of 25–100% did not demonstrate antibacterial activity against microorganisms in tuak samples. This study serves as a foundational evaluation of natural antibacterial agents and highlights the need for further optimization of extraction techniques or targeted testing on specific bacterial isolates.

This study aims to isolate and identify microorganisms in the form of bacteria and fungi in tilapia fish waste and to demonstrate the effectiveness of turmeric extract (Curcuma longa Linn.) as an antimicrobial agent. Tilapia fish waste samples were fermented for seven days and then microorganisms were isolated using Nutrient Agar (NA) media for bacteria and Potato Dextrose Agar (PDA) for fungi. Turmeric extract treatment was carried out using the disc diffusion method. The results showed the presence of several species of Gram-positive and Gram-negative bacteria, as well as fungal colonies with dominant characteristics of Aspergillus sp. and Fusarium sp. Turmeric extract with a concentration of 20% showed a significant inhibition zone against the growth of pathogenic bacteria and fungi. The content of secondary metabolites such as alkaloids, tannins, and phenolics in turmeric extract is thought to be the main factor in antimicrobial activity.

Fermentation is one of the traditional food processing techniques that not only functions as a preservation method, but also plays a role in improving the nutritional quality and safety of food. One example of a typical Indonesian fermented food that has survived from generation to generation is naniura, a traditional dish of the Batak Toba people made from carp (Cyprinus carpio) which is processed without a cooking process. The fermentation process in naniura is carried out spontaneously by soaking in a solution of citrus (Citrus Sp.) and a mixture of local spices, thus creating an acidic environment that supports the growth of fermentative microorganisms, especially lactic acid bacteria (LAB). The purpose of this study was to isolate and identify LAB from naniura, as well as to carry out morphological characteristics, Gram staining, and sensitivity tests to several commonly used antibiotics. Isolation was carried out using MRS media in anaerobic conditions, while sensitivity tests were carried out using the disc diffusion method. The results showed that isolates from naniura had Gram-positive characteristics, rod-shaped, catalase negative, and showed varying inhibition zones against antibiotics. Some isolates showed resistance to amoxicillin and cefataxim, but were sensitive to erythromycin and gentamicin. These results indicate that LAB from naniura has potential as a probiotic candidate, but it is necessary to monitor the possibility of antibiotic resistance gene transfer.  

Fungal infection is an infectious disease that has a fairly high prevalence in tropical countries. Fungi that cause infections include Candida albicans and Malassezia furfur. Sungkai plants (Peronema canescens) can be used as antifungals because they contain secondary metabolite compunds, such as flavonoids, tannins, and saponins. The pupose of this study was to determine the antifungal activities of 96% ethanol extract of sungkai leaves against Candida albicans and Malassezia furfur. The antifungal activity test of the extract used the disc diffusion method with test solution concentrations of 6.25%, 12.5%, 25%, and 50% with ketoconazole as positive control and sterile aquadest as a negative control. The best inhibition results were obtained at a concentration of 50% with an average inhibition zone of 9.8mm (moderate category) against Candida albicans and an average inhibition zone of 6.88mm (moderate category) against Malassezia furfur. This shows that ethanol extract of sungkai leaves has antifungal activities.

Cottage salak (Salacca zalacca (Gaertn.) Voss) is one of the tropical fruits that not only has high nutritional value, but also contains bioactive compounds that have the potential to be natural antibacterial. The skin of the salak pondoh fruit is often considered waste, even though this part is rich in chemical compounds such as flavonoids, saponins, phenols, tannins, alkaloids, chlorogenic acid, ferrulic acid, and protocatetic acid. These compounds are known to have biological activity that is able to inhibit the growth of pathogenic microorganisms. Escherichia coli and Salmonella typhi bacteria are the main causes of diarrhea and typhoid fever that affect many people, especially in areas with poor sanitation. Infection of these two bacteria can cause intestinal tissue damage, dehydration, and even serious complications if not treated immediately. Therefore, efforts to find alternative antibacterial agents from natural ingredients such as salak pondoh bark are important. This study aims to test the effectiveness of ethanol extract of salak pondoh bark against E. coli and S. typhi bacteria. Extraction was carried out by the maceration method using ethanol as a solvent, then continued with testing of antibacterial activity using the disc diffusion method. The concentration of the extract was tested on several variations to determine the minimum concentration capable of providing an optimal buffer zone against both test bacteria. The community service program integrated with this research aims to increase public understanding regarding the potential of salak pondoh skin as a natural antibacterial. Education is carried out through counseling and demonstrations of making simple extracts that can be used to clean household appliances or prevent microbial contamination of foodstuffs. With this approach, it is hoped that the community can use salak pondoh skin waste more productively, while reducing dependence on synthetic chemicals that have the potential to cause side effects.

Milkwood bark (Alstonia scholaris (L.) R. Br.) is one of the medicinal plants of the genus Alstonia. This plant is traditionally used by the community as a medicine for diarrhea, diabetes, malaria, hemorrhoids, and antihypertensives. This plant contains secondary metabolites that have the potential as antibacterial such as alkaloids, flavonoids, saponins and tannins. The purpose of this study was to determine the inhibition of antibacterial activity and the most effective concentration in inhibiting Staphylococcus aureus and Escherichia coli. The sample used in this study was milkwood bark, the sample was extracted by maceration method using 96% ethanol as solvent. The antibacterial testing method used in this study was disc diffusion. The study used a completely randomized design with 9 treatments, namely 1000 ppm, 750 ppm, 500 ppm, 250 ppm, 100 ppm, 50 ppm, 10 ppm, Ciprofloxacin (positive control), sterile distilled water (negative control). The results of the antibacterial activity test at a concentration of 500 ppm showed that milkwood bark extract could inhibit the growth of S. aureus by 8.22 mm and at a concentration of 250 ppm showed that milkwood bark extract could inhibit the growth of E. coli by 8.75 mm. From the test results, it can be concluded that milkwood bark (A. scholaris (L.) R. Br.) has antibacterial activity that has moderate potential in inhibiting S. aureus and E. coli.

Origanum majorana demonstrates a broad spectrum of effectiveness against various diseases, exerting antimicrobial effects against different pathogenic microorganisms. The goals of this research are to provide light on how Origanum majorana extract inhibits human pathogenic bacteria, how it may prevent biofilm development, and how it affects bacterial adhesion. The antibacterial activity of the water-based Origanum majorana extract was assessed in this research using two different methodologies. "Antibiotics were compared to its effectiveness using an agar-well diffusion assay and disc diffusion method," the first step. Additionally, the extract's ability to inhibit biofilm formation and bacterial adherence was assessed through specialized tests. All bacterial isolates of Gram negative, Gram-positive bacterial types were sensitive to Origanum majorana extract and the range of inhibition zone (30 to 24) mm. Although floxacin was effective against some of these isolates, the majority of them were resistant. Most bacterial Gram negative types were exhibit moderate adherence and biofilm activity to this extracts and some bacterial isolated of bacteria were exhibit high adherence and biofilm activity to the watery extracts of Origanum majorana. This research confirms previous findings that Origanum majorana extracts are very effective against a wide variety of clinical isolates of bacteria, including Gram-negative as well as Gram-positive varieties. Notably, the extracts were found to be more effective than commercially available antibiotics. Furthermore, the extracts displayed significant inhibition of bacterial adherence and biofilm formation. Based on these findings, Origanum majorana extracts have great promise as an effective antibacterial and anti-biofilm agent.

This study discusses the implementation of the Excise Services Information System (ExSIS) application at the Sidoarjo Customs and Excise Office as an innovation in public services. The objective is to evaluate the effectiveness of ExSIS in improving operational performance and provide recommendations for its development. This research employs a qualitative method with interviews conducted with Customs and Excise officers and service users, analyzed using Everett M. Rogers' diffusion of innovation theory. The results indicate that ExSIS improves time efficiency by up to 80% and reduces administrative errors by 70%. However, challenges such as user adaptation and the need for adequate infrastructure remain. In conclusion, ExSIS has enhanced efficiency and transparency in Customs and Excise administration. The study recommends continuous training, infrastructure upgrades, and internal policy revisions to maximize the application’s potential in supporting faster and more accurate public services.

This study discusses the scope of innovation diffusion in education in Indonesia. In essence, education plays a fairly essential role in shaping the quality of human resources that are able to face the challenges of the times. Along with global changes marked by technological developments and economic transformation, demands on the education system continue to increase. The researcher uses a Qualitative Literature Review which is carried out by the researcher reading and observing various written reference sources related to the scope of innovation in education in Indonesia. The results of this study indicate that overall, the process of innovation diffusion in education involves various complex stages and requires support from all parties involved in the education system. Diffusion of educational innovation in general can be concluded as a very complex process and is influenced by various specific factors, both internally and externally in the educational environment.