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The development of modern biotechnology has brought fundamental changes to the life sciences through the application of molecular biology, genetic engineering, and bioinformatics techniques. This article aims to examine the transformation of life sciences through modern biotechnology approaches and its impact on health, agriculture, the environment, and industry. The method used is a descriptive-analytical literature review based on international journal sources and relevant scientific publications. The results of the study indicate that technologies such as CRISPR-Cas9 enable precise gene editing for genetic disease therapy, while the mRNA vaccine platforms developed by Pfizer and Moderna demonstrate the acceleration of biomedical innovation in response to the global pandemic. In the agricultural sector, genetic engineering increases crop productivity and resilience, while in the environmental sector, biotechnology supports environmentally friendly bioremediation processes. However, ethical and regulatory challenges remain important concerns in its implementation. In conclusion, modern biotechnology plays a key role as a catalyst for sustainable, innovation-driven transformation in the life sciences.

Processing plastic waste into fuel oil with pyrolysis technology. Research on plastic oil as an alternative fuel has been widely conducted. One of them is research on processing plastic bag waste into fuel oil with pyrolysis technology. In this study, a set of pyrolysis equipment was made by Lecturers and Students of UMNU Kebumen. The plastic waste used in this study was 1 kg of plastic bag. Meanwhile, the pyrolysis process used a temperature of 250oC-300oC. The fuel oil produced in the pyrolysis process of this study was approximately 400 ml. The average density of fuel oil from plastic bag waste was 0.733 gr/ml. The results of this density calculation are in the possibility of the density of gasoline, namely 0.710 gr/ml to 0.770 gr/ml. This pyrolysis process shows great potential for converting plastic waste into an environmentally friendly alternative energy source. Furthermore, the efficiency of this pyrolysis technology can be improved by adjusting the temperature and processing time, as well as by selecting a wider variety of plastic types. This technology has the potential to be applied more widely in plastic waste management within the community as a solution to reduce environmental pollution while generating renewable energy.

The danger of cadmium contamination in water sources remains a crucial environmental issue due to its persistent nature and high toxicity level, which poses serious risks to human health and ecosystems. Cadmium is a non-biodegradable heavy metal that can accumulate in living organisms over time. The presence of these toxic Cd²⁺ ions is known to trigger damage to vital organs such as the liver and kidneys; therefore, reducing their concentration in aquatic environments is of paramount importance for environmental protection and public health safety. Among various treatment methods, adsorption is considered one of the most effective and economical techniques for removing heavy metal ions from contaminated water. In this study, the capability of mesoporous silica MCM-48-NH₂ as an adsorbent for Cd²⁺ ions was systematically evaluated. The adsorption performance was examined by investigating several important parameters, including contact time, solution acidity level (pH), and initial Cd²⁺ concentration. Furthermore, the adsorption mechanism and interaction between Cd²⁺ ions and the adsorbent surface were analyzed using Langmuir and Freundlich isotherm models. The results demonstrate that the adsorption process of Cd²⁺ ions onto MCM-48-NH₂ tends to follow the Langmuir isotherm model, indicating monolayer adsorption behavior, with a maximum adsorption capacity of 0.66 mmol g⁻¹.

Lemongrass (Cymbopogon citratus) is a plant known to contain various bioactive compounds with potential antioxidant properties as well as xanthine oxidase (XO) inhibitory activity. This study aimed to evaluate the phytochemical content, antioxidant activity, and XO inhibitory potential of ethanol extracts from the leaves, stems, and their combination. Phytochemical screening revealed that all extracts contained alkaloids, flavonoids, and terpenoids, while phenolic compounds were detected only in the leaf extract and the combined leaf–stem extract. Antioxidant activity assays demonstrated that all extracts exhibited very strong antioxidant activity, with IC₅₀ values below 50 ppm, indicating a significant potential to scavenge free radicals. In the XO inhibition assay at a concentration of 200 ppm, the ethanol extract of lemongrass stems showed the highest inhibitory activity at 81.37%, followed by the leaf extract at 48.08% and the combined leaf–stem extract at 33.65%. Overall, these findings suggest that the ethanol extract of lemongrass stems is the most promising natural source of antioxidants and has the greatest ability to inhibit xanthine oxidase activity, indicating its potential development as a functional ingredient for health applications.

This study comprehensively evaluates the quality of biomass briquettes produced from blends of coconut shell charcoal and sengon sawdust, using both carbonized and non-carbonized materials. Composite briquettes were fabricated with varying compositions and characterized through proximate analysis, calorific value, density, and burning rate measurements to determine their suitability as solid fuel. The results indicate that adding non-carbonized sawdust increases volatile matter content and burning rate but reduces the calorific value of the briquettes. In contrast, incorporating up to 10% carbonized sawdust significantly improves the calorific value to 6119.2 cal/g, approaching that of pure coconut shell charcoal (6352.2 cal/g), while maintaining a relatively high burning rate. Briquettes containing carbonized sawdust also exhibit low ash content, below 3%, and moisture content under 8%, meeting standard solid fuel quality requirements. These findings demonstrate that a strategic combination of carbonized and non-carbonized materials can produce hybrid biomass briquettes with optimized thermal performance, providing a promising, sustainable, and environmentally friendly alternative fuel for domestic and industrial applications.

The discharge of surfactant-laden wastewater from the rapidly expanding laundry industry poses significant environmental risks, especially in densely populated urban areas. While constructed wetlands (CWs) and Eco-Enzyme technology have shown promise for surfactant remediation, their standalone application requires long hydraulic retention times (HRTs), limiting practical implementation. This study evaluated the efficacy of a novel integrated system combining a subsurface constructed wetland (SSFCW) with fruit peel-derived Eco-Enzyme to treat synthetic laundry wastewater. Over a 6-day treatment period, the combined system achieved a remarkable surfactant removal efficiency of 99.63%, reducing the concentration from 225 mg/L to 0.835 mg/L—well below the regulatory threshold of 3 mg/L. The synergistic degradation mechanism involves enzymatic hydrolysis via Eco-Enzyme lipase and protease activity, complemented by microbial mineralization in the wetland rhizosphere. This system maintains optimal environmental conditions, with a stable pH of 6.85-7.32 and a temperature of 30.9-35.2°C, supporting robust biological activity. These findings demonstrate that the integrated Eco-Enzyme/SSFCW system overcomes the limitations of conventional HRT approaches, offering a highly efficient, sustainable, and practical decentralized wastewater treatment solution for the laundry industry.  

Histology practicum, as one of the compulsory practicum courses, offers a great opportunity to practice various aspects of science process skills through microscopic tissue observation activities, identification of cell and tissue structures, and analysis of tissue functions in organisms. This study aims to analyze the science process skills biology education students through histology practicum activities. This study uses a quantitative descriptive approach with a survey method to analyze the science process skills of biology education students through histology practicum activities. Data collection in this study used three main techniques, namely science process skills tests, direct observation, and analysis of practicum report documents. Furthermore, the data were analyzed descriptively quantitatively using descriptive statistics to describe the profile of students' science process skills. Analysis per aspect revealed that students had good skills in the aspects of observing (78.50%), classifying (76.33%), interpreting data (71.67%), applying concepts (73.25%), and communicating results (73.40%), which indicates that histology practicum is effective in developing basic science process skills. However, the aspects of formulating hypotheses (58.17%) and planning experiments (54.83%) were still in the sufficient category, indicating that students experienced difficulties in integrated science process skills that require higher-order thinking skills. The findings this study provide important implications for the development of more comprehensive histology practicum learning strategies, including the integration of inquiry approaches, the use of continuous formative assessment, the application of differentiated learning, and strengthening the connection between theory and practice to optimize the development of all aspects of science process skills of prospective biology teachers.  

The increasing demand for paper has led to a shortage of wood-based raw materials, thus requiring environmentally friendly alternatives. This study aims to analyze the physical, mechanical, and morphological characteristics of paper made from cassava peels (Manihot esculenta Crantz) and oil palm fruit fibers (Elaeis guineensis Jacq.) as eco-friendly paper raw materials. The research method involved pulp preparation through delignification using 3.5% HNO₃ and 3% NaOH, bleaching with 10% H₂O₂, paper sheet formation with varying compositions (A = 70:30, B = 50:50, C = 30:70, D = 10:90), followed by grammage testing and tensile strain testing. The results showed that the grammage values ranged between 96.3–99.7 g/m², while tensile strain ranged between 2.6–4.4%. The study concluded that the best composition was found in sample C (30% cassava peel : 70% palm fiber) with a grammage of 98.5 g/m² and tensile strain of 3.2%. The implication of this study is that cassava peel and palm fiber waste have the potential to serve as alternative paper raw materials that meet the quality standards of A-grade printing paper (SNI 7274:2008), while also supporting the reduction of dependence on wood.

Low student achievement in mathematics, particularly in arithmetic sequences and series, has led to decreased learning motivation and participation. Therefore, innovative and engaging instructional strategies are required, one of which is the implementation of ice-breaking activities to reduce boredom and enhance students’ learning engagement. This study aimed to examine the effect of implementing ice breaking on the mathematics learning outcomes of tenth-grade students at SMK Kesehatan Samarinda in the topic of arithmetic sequences and series. This research employed an experimental method using a quasi-experimental design, involving two groups: an experimental group that received instruction incorporating ice breaking and a control group that did not receive such treatment. The total sample consisted of 60 students selected through purposive sampling. The research instrument was a learning achievement test administered as a pretest and posttest. Data were analyzed using a paired sample t-test to determine differences in learning outcomes between the two groups. The results revealed a significance value of 0.000 (< 0.05), indicating a significant difference in learning outcomes between the experimental and control groups. Therefore, it can be concluded that the implementation of ice breaking has a significant effect on improving students’ mathematics learning outcomes. This strategy creates a more active, interactive, and enjoyable learning environment, thereby facilitating students’ understanding of mathematical concepts, particularly arithmetic sequences and series.

This study discusses the implementation of the Self-Directed Learning Programme by integrating Learning Outcomes (CP), Learning Objectives (TP), and Learning Objective Processes (ATP) to enhance teaching quality and teacher professionalism. Using a library research method, this study analyses relevant scientific literature to understand the relationship between the integration of the three components of the education programme and classroom teaching practices. The research findings indicate that teachers still encounter challenges in understanding and integrating CP, TP, and ATP, particularly in planning, implementing teaching strategies, and conducting authentic assessments. However, when this integration is well executed, the teaching process becomes more purposeful, flexible and responsive to students' needs. Furthermore, integrating CP–TP–ATP has been shown to strengthen teachers' professionalism by enhancing their understanding of the curriculum, their ability to design learning activities, and their capacity to conduct competency-based assessment. This study confirms that the successful implementation of the Free Education Programme depends heavily on teachers' ability to understand the curriculum's structure and to integrate these three components consistently. Therefore, training, supporting, and developing teachers' competencies is an important requirement for the effective and sustainable implementation of the Liberal Education Programme.

Rainfall plays a crucial role in determining flood risk, particularly in regions with high precipitation intensity and limited drainage capacity. Langkat Regency in North Sumatra is one of the areas frequently affected by seasonal flooding. This study aims to model the spatial distribution of rainfall and estimate the rainwater volume using the double integral approach as a basis for flood mitigation planning. Monthly rainfall data from various observation stations in 2024 were processed to obtain the average rainfall intensity, which was then converted into meters and multiplied by the total area of Langkat Regency to compute the rainwater volume. The results indicate that the total estimated rainwater volume throughout 2024 reached 16,409,819,800 m³, with peak precipitation occurring from September to November, contributing significantly to the increasing flood risk in low‐lying zones and riverine areas. These findings demonstrate that the use of double integrals is an effective quantitative method for predicting potential flood volume based on rainfall distribution. The outcomes of this study are expected to serve as a scientific reference for local governments in developing data-driven flood mitigation strategies, such as improving drainage capacity, constructing retention basins, and strengthening watershed management.

This study aims to analyze the implementation of video animation-based flipped classroom learning model in increasing students' learning interest in science subjects with material on the structure and function of living organisms in class 8D at SMPN 1 Sanrobone. The low interest in learning science among students is a major problem that needs to be addressed through more attractive and interactive learning innovations. The video animation-based flipped classroom model was chosen because it can optimize classroom learning time for more meaningful activities, while basic concept understanding is done independently through engaging animated videos. This study uses a survey method with a descriptive quantitative approach. The research subjects were  students of class 8D SMPN 1 Sanrobone in the even semester of the 2024/2025 academic year. Research instruments used learning interest questionnaires, observations, and documentation. The results showed that the implementation of video animation-based flipped classroom model was able to increase students' learning interest with high categories in aspects of attention, interest, involvement, and learning satisfaction. Animated videos presented before face-to-face learning provide clear visualizations of the structure and function of living organisms, making students more prepared and enthusiastic to participate in discussions and practical activities in class. This learning model also encourages student learning independence and increases active interaction in learning. This study recommends the implementation of video animation-based flipped classroom as an alternative effective and innovative science learning strategy to increase students' learning interest.

Chili pepper (Capsicum frutescent L.) is known as one of the high sources of vitamin C, which acts as an important natural antioxidant for human health because the body is unable to produce its own. This study aims to analyse vitamin C levels in chili pepper using the iodometric titration method quantitatively by taking a 5 gram sample of chili pepper and titrating it using 0,1 N iodine solution. The analysis results showed that the vitamin C content obtained was 0,0704% or equivalent to 70,4 mg/100g. This value is higher than some research results. This value is higher than some previous research results on fruits and chillies from other regions. This finding shows that chilli pepper has great potential as a source of vitamin C in supporting the fulfilment of people’s daily nutrition. In addition, the iodometric titration method is proven to be effective and efficient to be used in the analysis of vitamin C in foodstuffs. Therefore, chili pepper consumption can be a simple strategy in improving nutritional and preventing disease related to vitamin C deficiency.

This research extends the previous work of Tsaqila, Winiarti, and Widaningrum (2024), who applied the Complex Proportional Assessment (COPRAS) method within a decision support system for supermarket branch location selection. Unlike the prior study, which focused on Ponorogo through a web-based framework, this study expands the implementation of COPRAS to the Medan Area, Medan Kota, Medan Polonia, dan Medan Maimun districts, adapting it to local urban, social, and economic characteristics. The main objective is to identify the most strategic site for a new supermarket by analyzing multiple criteria, including land cost, population density, accessibility, safety, cleanliness, and disaster risk. Data were collected from both field surveys and official government publications. The findings reveal that the COPRAS method provides reliable and objective assessments among the evaluated alternatives, with Medan Area emerging as the most suitable location for supermarket development. Overall, this study broadens the practical scope of the COPRAS method in a different regional context and reinforces its reliability and adaptability as a multi-criteria decision-making tool in the modern retail industry.

Research. Elementary School Teacher Education Study Program, STKIP PGRI Papua. The purpose of this study is to examine the Influence of Using Kahoot as an Interactive Learning Media to Increase the Learning Interest of Sixth-Grade Students in Civic Education at SD YPK Bethania Mariadei. This type of research is Experimental research. The population in this study consists of students at SD Bethania Mariadei, while the research sample is 21 students in the sixth grade. Data collection was conducted through questionnaires and documentation. The collected data was analyzed using descriptive statistics and inferential statistics techniques. Based on the hypothesis testing results, the calculated t-value is 2.926 with a significance of 0.009. The significance value shows 0.009 < 0.05, so H0 is rejected and H1 is accepted, which leads to the conclusion that there is a significant and positive effect of using Kahoot media as an interactive learning tool to increase the learning interest of sixth-grade students in Civics subjects at SD YPK Bethania Mariadei. The Kahoot Interactive Learning Media can be applied for Civics learning in elementary schools. Keywords: Kahoot, Learning Media, Learning Interest, Civics.  

PT. Tirta Fresindo Jaya, specifically its Pasuruan plant as the producer of Pucuk Harum Tea beverage, requires a large supply of steam to support various production processes. This steam is used in the process of heating tea leaves, heating water through a heat exchanger, and heating chemicals and water in the cleaning in place (CIP) process. Steam pressure of 5 bar that is flowed to the process equipment will undergo condensation to produce condensate. To separate steam and condensate, steam traps are used, consisting of several types, namely mechanical, thermostatic, and thermodynamic. However, in operational practice, steam trap malfunctions often occur, either in the form of steam leaks that are wasted with condensate or failure to drain condensate from the system. This condition causes a decrease in the efficiency of the steam piping system and increases the workload of the boiler. As a result, fuel consumption and boiler feed water requirements become greater than ideal conditions. Therefore, this study aims to analyze the energy and operational losses caused by steam trap malfunctions, as well as evaluate their impact on boiler system performance and steam utilization efficiency at PT. Tirta Fresindo Jaya Pasuruan plant.      

This research is motivated by the persistent difficulty elementary school-aged children have in controlling their emotions and behavior in social situations, particularly within the context of church community development. The study aimed to examine the effect of Christian values-based emotion management training on improving children's self-control. The study used a quantitative approach with a quasi-experimental one-group pretest-posttest design on 30 children aged 7–12 years at the GPM Wonreli–Kisar Congregation. The intervention was implemented over four days through activities involving emotion recognition, value reflection, emotion regulation training, and social simulations. Data were collected using a self-control scale and analyzed using paired-difference tests and effect size calculations. The results showed a significant increase in self-control scores after the training, with the largest changes in rule compliance and impulse control, and a more moderate increase in resilience to frustration. The effect size indicates a strong practical impact of the changes. These findings indicate that training that integrates social experiences and value reflection can facilitate the shift in behavioral control from external control to internal awareness. Practically, this research provides a basis for developing a community-based child development program that emphasizes not only discipline but also the understanding of values ​​in character formation and self-regulation.

This quasi-experimental study examines the effectiveness of Artificial Intelligence (AI)-assisted learning in enhancing critical thinking skills among undergraduate history students. The study involved 60 students divided into experimental and control groups. The experimental group received AI-supported instruction integrating adaptive learning modules, scaffolded source-analysis prompts, and guided argumentative discussions facilitated by conversational AI tools, while the control group followed conventional lecture-based instruction. Data were collected using a validated critical thinking test, classroom observation protocols, and semi-structured interviews. Quantitative data were analyzed using paired and independent sample t-tests, while qualitative data were examined through Miles and Huberman’s interactive analysis model. Results indicate statistically significant improvements in critical thinking scores in the experimental group compared to the control group. Thematic findings reveal enhanced sourcing, contextualization, corroboration, and evidence-based argumentation skills. However, minor risks of over-reliance on AI highlight the need for instructional scaffolding and ethical guidance. The findings suggest that AI can function as a cognitive scaffold that strengthens historical thinking and metacognitive awareness when implemented within a structured pedagogical framework.

Vocational education in Vocational High Schools (SMK) requires innovative, interactive, and industry-relevant learning media to support students’ independent and competency-based learning. However, learning activities in the subject Communication in the Workplace for Grade XI MPLB at SMKN 62 Jakarta are still dominated by lecture-based methods with limited instructional media, resulting in low levels of student independence and understanding. This study aimed to develop and examine the feasibility, practicality, and effectiveness of an interactive e-book based on Canva for Oral Communication materials in Indonesian Language. The study employed a Research and Development (R&D) method using the ADDIE development model, consisting of analysis, design, development, implementation, and evaluation stages. The research subjects included 36 students in the needs analysis stage, 5 students in the small-scale trial, and 31 students in the large-scale trial. Data were collected through observation, questionnaires, learning outcome tests, and documentation, and analyzed using descriptive qualitative and quantitative techniques. The validation results indicated that the Canva-based interactive e-book was categorized as very feasible, with validation percentages of 100% from the material expert, 88.24% from the media expert, and 95% from the language expert. The small-scale trial showed a practicality percentage of 82.2%, while the large-scale trial reached 88.39%, both classified as very practical. In addition, the pre-test and post-test results in the large-scale trial demonstrated an increase in students’ average scores and a higher number of students achieving the Minimum Mastery Criteria (KKM). Therefore, the Canva-based interactive e-book is feasible, practical, and effective as a learning medium to enhance students’ understanding, motivation, and independent learning in Oral Communication for vocational high school students.

State-based conservation in Indonesia often faces institutional failure and social resistance, resulting in continued deforestation. Conversely, Indigenous conservation models in West Java, specifically the Leuweung Larangan (forbidden forest), demonstrate significant ecological resilience. However, the legal standing of these customary practices within Islamic jurisprudence remains under-theorized, creating a dichotomy between Adat (custom) and Sharia. This study proposes a juridical reconstruction of the Sundanese taboo mechanism (Pamali) as Jarimah Ta’zir (discretionary sanction) to strengthen environmental law enforcement. Utilizing a socio-legal approach and ecological hermeneutics, this research analyzes Yusuf al-Qardhawi’s concept of Fiqh al-Bi’ah and Seyyed Hossein Nasr’s sacred science, alongside relevant empirical data on Indigenous forest governance. The findings demonstrate that Leuweung Larangan structurally manifests as Hima Syar’i (sacred protected zone). Operationally, Pamali functions not merely as a cultural myth, but as a preventive legal instrument (Sadd al-Dzari’ah) where environmental violations constitute religious criminal acts. This creates a "Sundanese Eco-theology" model that establishes a double-layered compliance system—social sanctions and theological liability—proving more effective for carbon preservation than state regulations alone. The study recommends integrating this model into the Green Constitution framework to resolve tenurial conflicts and enhance climate resilience.