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Long fishing is an environmentally friendly traditional fishing tool that is commonly used by fishermen to catch fish passively using bait, both in sandy waters and in coral reef areas. Grouper fish (Epinephelus sp.) tend to inhabit waters of a certain depth, especially in areas of coral fractures and dead corals that serve as shelter and foraging. Determining the right depth setting in the operation of a fishing rod is essential to optimize the catch. This study aims to determine the effect of the difference in setting depth on grouper fish catch in the waters of Pengambengan Beach and determine the most effective depth. The study used an experimental method with three depth treatments, namely 10 m, 15 m, and 20 m, each repeated nine times. Data analysis was carried out by Group Random Design (RAK) and ANOVA test. The results showed that a depth of 10 m produced the highest catch with a total of 35 fish (average 3.89 fish/trip), followed by a depth of 15 m with 10 fish (average 1.11 fish/trip), and a depth of 20 m with 7 fish (average 0.78 fish/trip). Statistically, the difference in depth has a real influence on the number of grouper catches. A depth of 10 m is recommended as the optimal setting for the operation of long fishing rods in the waters of Pengambengan Beach.

The development of molecular biology has significantly transformed clinical laboratory diagnostics, particularly through DNA analysis. One of the most widely used techniques is the Polymerase Chain Reaction (PCR), a DNA amplification method capable of rapidly, sensitively, and accurately multiplying specific genetic fragments. PCR has become an essential diagnostic tool for detecting infectious, genetic, and malignant diseases, even at early stages when the amount of target DNA is minimal. This article aims to analyze the role of PCR in DNA analysis as a diagnostic tool in clinical laboratories, including its working principles, advantages, and relevance in modern clinical practice. This study employs a literature review approach by examining various scientific sources such as international journals and molecular biology textbooks. The findings indicate that PCR offers high sensitivity and specificity compared to conventional methods, thereby improving diagnostic accuracy and accelerating appropriate therapeutic decisions. Therefore, PCR represents a crucial innovation in clinical laboratory diagnostics that significantly contributes to improving healthcare quality.

Learning concentration is an important factor in supporting students’ academic success, particularly in Biology study programs that require high levels of accuracy and analytical skills. Learning concentration is influenced by various factors, including sleep quality and dietary patterns. This study aimed to analyze the relationship between sleep quality and dietary patterns and the level of learning concentration among Biology students of class T.BIO1/2022 at Universitas Islam Negeri Sumatera Utara. This study employed an analytic observational approach with a cross sectional design. The research sample consisted of 23 students selected using a total sampling technique. The research instruments included questionnaires on sleep quality, dietary patterns, and learning concentration. Data analysis was conducted descriptively and using cross tabulation. The results showed that students with good sleep quality and regular dietary patterns tended to have higher levels of learning concentration compared to those with poor sleep quality and irregular dietary patterns. Therefore, sleep quality and dietary patterns are important factors that need to be considered to support students’ learning concentration.

Biodiversity represents the condition and level of ecosystem complexity in a particular area, reflecting the presence of various living organisms and their interactions with the environment. Based on its ecological conditions, Mooat Lake in East Bolaang Mongondow Regency serves as a habitat for various plant species, one of which is moss (Bryophyta), which plays an important role in maintaining ecosystem balance. The presence of Bryophyta in this area has the potential to be utilized as a contextual learning resource in biology education. This study aims to determine the validity level of an interactive e-book based on Bryophyta research conducted at Mooat Lake, East Bolaang Mongondow Regency, as a biology learning material for tenth-grade senior high school students. This research employed a Research and Development (R&D) method using the ADDIE development model, which consists of the stages of analysis, design, development, implementation, and evaluation. The product developed in this study was an interactive e-book containing biodiversity material based on field research findings. The results showed that the material expert validation obtained a percentage of 94.2%, categorized as very valid, while the media expert validation obtained a percentage of 83.7%, also categorized as very valid. Based on these findings, it can be concluded that the interactive e-book based on moss research meets the criteria of being very valid and is suitable for use as a learning resource in biology subjects, particularly on biodiversity topics.

The decline in seed quality due to storage beyond the shelf life is a major problem in rice cultivation. One effort that can be made to improve seed viability is through invigoration techniques using natural ingredients, such as bean sprout extract (Phaseolus radiatus), which contains growth hormones. This study aims to analyze the effect of bean sprout extract on the viability of Ciherang rice seeds that have exceeded their shelf life and to determine the best treatment. The study was conducted from August to November 2024 at the Biology Laboratory of the UPTD Seed Center, Supervision and Certification of Agricultural Seeds of Gorontalo Province. The study used a two-factor Randomized Block Design (RBD) with bean sprout age (3, 5, and 7 days after sowing) and bean sprout extract concentration (20 g/L, 40 g/L, and 60 g/L) as factors, with four replications. The parameters observed included germination rate, maximum growth potential, and sprout length. The data were analyzed using ANOVA and DMRT post-hoc test at a 5% level. The results showed that bean sprout extract had a significant effect on all observed parameters. The treatment of 5-day-old bean sprouts with a concentration of 20 g/L gave the best results with a germination rate of 95.5%, maximum growth potential of 98.5%, and the highest sprout length. Sprout extract has the potential to be used as a natural alternative to improve the quality of rice seeds that have passed their storage period.

This community service aims to provide understanding and skills in using comics and AR media as well as understanding biology material by high school students. The community service was carried out at SMA Negeri 1 Raja Ampat involving 43 students. Measurement of student responses using a questionnaire with six statements on a scale of 1 - 4. The measurement data was calculated to obtain the average score and percentage of student responses. The results of the community service show that the average total score is 86.53 which indicates that overall the student response is very good to the community service activities that have been carried out. Student responses are dominated by agree and strongly agree. The largest percentage of disagree, namely 23.26%, is shown by students, especially in the statement that AR applications can be run easily, but the average total score still reaches the good category for this statement. The community service has provided students with an understanding of how to learn biology in an interesting way and provides benefits to improve their understanding. Students agree that the material presented adds to their insight regarding comics and AR, are able to install AR applications on their mobile devices, improve their understanding of biology material, is interesting and provides benefits for them.

This research is motivated by challenges in biology learning, including the lack of digital literacy assessment in schools and the use of conventional learning methods that tend to make students bored. Impractical printed teaching materials cannot be integrated into the form of videos. This research aims to develop an e-module based on Science, Technology, Engineering, and Mathematics (STEM) to analyze the feasibility, practicality, and effectiveness of e-modules in improving students' understanding of concepts and digital literacy on human respiratory system materials. This research uses the Development Research (R&D) method with the ADDIE (Analysis, Design, Development, Implementation, Evaluation) development model.  The research was carried out at SMAN 1 Malinau. The implementation stage used a non-equivalent control group  design involving 34 students in the experimental class and the control class. Data collection instruments include validation sheets for media and material experts, practicality questionnaires for teachers and students, and pretest-posttest  tests to measure concept understanding and digital literacy.  Analysis of effectiveness data using N-gain test and hypothesis test (independent sample t-test for concept understanding and Mann-Whitney for digital literacy). The results of the research based on the validation of media experts and subject matter experts show that the STEM-based e-module is declared "Excellent". The e-module is also rated "Very Practical" by biology teachers and students. The e-module was shown to be effective in improving conceptual understanding, where the average N-gain value of the experimental class (58.63; Quite Effective category) was significantly higher (p=0.017) than that of the control class (40.62).

This research aims to: (1) differentiated biology learning tools with ethnoscience content, (2) determine the practicality of differentiated biology learning tools with ethnoscience content and (3) determine the effectiveness of differentiated biology learning tools with etnoscience content in improving critical thinking skills and environemantal literacy of phase E student. This study is a development research using the Design and Development Research model, which adopts the ADDIE development model comprising five stages: (1) analysis, (2) design, (3) development, (4) implementation, and (5) evaluation. The research method used is the Quasi-Experimental Design with a Nonequivalent Control Group Design approach. Data analysis includes normality tests, Levene's homogeneity test, and the Normalized Gain Score (N-Gain Score) test. The limited trial involved 30 eleventh-grade students from SMA Negeri 1 Simpangkatis. The field trial involved tenth-grade students from the same school, consisting of 31 students in the experimental class and 30 students in the control class. The data collection instruments consisted of questionnaires to gather responses from experts, teachers, and students regarding the biology learning tools, as well as tests to measure students' critical thinking skills and environmental literacy. Instrument validity was ensured through content validation by subject matter experts and media experts. The practicality of the learning tools was evaluated based on feedback from teachers and students. The results of the study showed that: (1) the developed biology learning tools were rated as highly feasible by experts; (2) the tools were considered highly practical by both teachers and students; and (3) the tools were effective in improving students' critical thinking skills and environmental literacy. This effectiveness was supported by the average gain score analysis in the experimental class, which was 0.71 for critical thinking skills (high category) and 0.57 for environmental literacy (moderate category).

This study aims to describe the application of biology learning control based on the value of love at SMK Kesehatan Athallah Putra Palembang. The concept of control applied not only focuses on formal supervision but also emphasizes a humanistic guidance approach, which includes attention, emotional support, and motivation for students. This research uses a descriptive qualitative method with data collection techniques such as observation, interviews, and documentation. The results show that the implementation of love-based control creates a harmonious, interactive, and comfortable learning environment. Teachers act not only as supervisors but also as mentors who play a role in fostering positive character and enhancing student learning motivation. With this approach, better relationships between teachers and students are formed, encouraging students to be more active in the learning process. Love-based control is proven to be effective in improving the quality of biology learning at SMK Kesehatan Athallah Putra, while also instilling positive humanitarian values in the school environment. This study shows that the application of control focused on love can have a positive impact on the quality of education.

Biology education in high school has an important role in shaping students' science literacy and critical thinking skills. However, ecological material, particularly the topic of Ecosystem Components and their Interactions, is often considered difficult because it is abstract and not easy to observe directly. This research aims to improve student learning outcomes through the application of the Problem Based Learning (PBL) model, which emphasizes real problem-solving, collaborative discussion, and active involvement in the learning process. The research uses the design of Classroom Action Research (PTK) which was carried out in two cycles in class X of St. Rosa de Lima Catholic High School Tondano involving 10 students in the odd semester of the 2025/2026 school year. Data was collected through observation of teacher and student activities, learning outcome tests in the form of pretests and posttests, and learning documentation. The analysis was carried out descriptively, qualitatively, and quantitatively by calculating the average score, percentage of completion, and student involvement. The results of the study showed a significant increase. The percentage of teacher activity increased from 58.3% in the first cycle to 87.5% in the second cycle, while student activity increased from 45.83% to 83.33%. The average score of students also increased from 70% in cycle I to 90% in cycle II, with classical completeness reaching 90%. These findings confirm the effectiveness of PBL as a contextual Biology learning strategy, able to bridge ecological concepts with real life, and increase students' curiosity, cooperation, and critical thinking skills.

This classroom action research evaluates the effectiveness of Problem Based Learning in learning Biology on the topic of environmental change at Rosa de Lima Catholic High School Tondano, with the aim of improving the activities and learning outcomes of grade X students. The method used is two action cycles with stages of planning, implementation, observation, and reflection. The subjects are 10 students. Tools include Teaching Modules and Student Worksheets; Data was collected through observation sheets of teacher and student activities (scales 1–4) and 10-item learning outcome tests each cycle. The descriptive analysis calculates the grade average and classical completeness against the Minimum Completeness Criterion of 75. The results showed consistent improvement from cycle I to cycle II. Classical completeness increased from 66% (6 out of 9 students attended; average 76.67) to 90% (9 out of 10 students; average 81.00). The teacher's activity observation score increased from 3.75 to 3.91, while the student's activity from 3.50 to 3.60. The increase is fueled by the formulation of contextual problems, facilitation of guiding questions, individualized feedback, and more disciplined time management, which strengthens engagement, collaboration, and scientific reasoning. The findings indicate that Problem Based Learning is effective in improving Biology learning activities and outcomes on the topic of environmental change. This study adds to the empirical evidence in the context of high schools with low initial completeness and suggests further exploration of other Biology topics along with measurements of critical thinking skills and scientific attitudes for a more comprehensive picture of impact.

Literacy skills play an important  role in helping students clarify basic concepts, build relationships between concepts, reason and test each detail of each interrelated information and are able to derive each concept, thus students are accustomed to understanding each scientific process and activity carried out in Biology learning. The relationship between these two skills trains students to actively carry out in-depth scientific investigations.The purpose of this study was to identify the relationship between scientific literacy skills and scientific explanation skills in empowering scientific reasoning skills through the application of the Remap-NHT (Reading Concept Map-Numbered Head Together) learning model. This study included a quasi-experiment using a non-equivalent pretest-post-test control group design with a total sample of 85 eleventh grade students in the Natural Sciences department at SMAN 4 Malang in the 2023/2024 academic year. The population was distributed into 3 groups. Scientific literacy skills and scientific reasoning skills were assessed using an essay test, while scientific explanation skills were assessed using an assessment rubric. Data analysis was tested using multiple regression analysis. The results showed that (1) there was a significant relationship between scientific literacy skills and scientific reasoning skills; (2) there is a significant relationship between scientific explanation skills and scientific reasoning skills; and (3) there is a significant relationship between scientific literacy skills and scientific explanation skills that have an integral effect on scientific reasoning skills. The interrelationship between scientific literacy skills, scientific explanation skills and scientific reasoning skills can be observed in the regression equation Y = 0.317 * X1 + 0.995 * X2 + 30.113. Based on the findings, it is concluded that Remap-NHT is able to improve students' scientific literacy skills, scientific explanation skills, and scientific reasoning skills, the interrelationships of which influence each other.

Solid waste from the tapioca processing industry, which is based on cassava (Manihot esculenta), is a medium rich in organic matter, particularly starch, that supports the growth of various types of microorganisms, especially fungi and bacteria. This study aims to isolate and observe the morphology of microorganisms from solid waste of cassava from the tapioca industry using Potato Dextrose Agar (PDA) medium in a series of pharmaceutical microbiology practicum. Waste samples were taken from the tapioca processing site, then underwent a serial dilution process and inoculation into PDA medium, which was incubated at 28–30°C for 5 days. The isolation results showed the growth of microorganisms with diverse colony morphologies, which are suspected to originate from the genera Aspergillus, Penicillium, and Rhizopus, based on macroscopic and microscopic characteristics. The presence of these microorganisms indicates the potential utilization of cassava waste as a source of microbial isolates for biotechnological and pharmaceutical applications, such as enzyme production and development of bioactive compounds. This practicum activity also contributes to increasing students' understanding of isolation techniques, microorganism characterization, and their role in biological-based waste management.

Bulian coconut chips are a typical Jambi food made from dried and fried old coconuts. The traditional production method and inadequate hygiene standards make this product susceptible to microbial contamination, especially harmful bacteria. This study aims to identify the types of bacteria that may be present in Bulian coconut chips using culture and Gram staining methods as the initial step for morphological identification. Samples were taken from three local producers in Bulian District, Batanghari Regency, Jambi. Testing was carried out by serial dilutions up to 10⁻⁴, then planted on Nutrient Agar (NA) media and incubated for 24 hours at 37°C. The colonies that grew were then observed morphologically and Gram staining was performed using a modified method without alcohol. The results showed the presence of various colonies with cream, white, and pale yellow colors. Based on the results of Gram staining, bacilli and cocci were found, which were included in the Gram-positive and Gram-negative categories. The types of bacteria suspected to appear include Bacillus sp., Staphylococcus sp., and possibly Escherichia coli. These findings indicate a risk of microbial contamination that could endanger consumer health, especially if the production process is not improved. Strict hygiene and sanitation principles are strongly recommended in the processing of this traditional food to ensure the product remains safe for consumption. The importance of implementing hygienic standards in the production of Bulian coconut chips is further emphasized by these findings. In addition to improving food safety, implementing proper sanitation procedures can also extend the product's shelf life and increase consumer confidence in local traditional foods. Efforts such as training local producers, using clean equipment, and hygienic packaging can help minimize the risk of bacterial contamination.

The topics of morphology, anatomy, and physiology are integral to biology learning, yet they often present challenges for students due to their abstract and complex nature. Difficulties in understanding body structures and invisible biological processes lead to low student interest and engagement. This study aims to identify innovative learning approaches through an analysis of national scientific literature over the past decade. The results show that the integration of digital technology and student-centered learning methods can improve conceptual understanding and learning motivation. The most prominent innovations include the use of VR and AR-based media to clarify visualizations, the use of interactive videos and simulations to explain biological functions, and the application of contextual learning models such as Problem-Based Learning (PBL) and Project-Based Learning (PjBL). These approaches have proven effective in creating a more immersive learning experience that is relevant to students' needs.

Improving the quality of biology learning in secondary schools requires pedagogical innovations that connect academic content with real-life contexts and leverage digital technology. This study aims to analyze the effect of a contextual teaching approach assisted by interactive PowerPoint on students’ learning outcomes in the human respiratory system topic. A true experimental design with a pretest–posttest control group was employed at SMA Negeri 1 Langowan, involving two eleventh-grade classes selected through simple random sampling. The experimental class received contextual instruction integrated with interactive PowerPoint, while the control class was taught using conventional methods. Data were collected through validated achievement tests and analyzed using normality and homogeneity tests, followed by independent and paired t-tests. The results indicate a significant improvement in learning outcomes for students in the experimental class compared to the control group. The experimental group’s posttest scores were substantially higher, and the difference was statistically significant. Integrating a contextual approach with interactive visualization effectively enhanced students’ understanding of abstract biology concepts, promoted active participation, and strengthened learning motivation. These findings support constructivist theory and highlight the relevance of contextual teaching in science education aligned with the Merdeka Curriculum. The study implies the importance of implementing context-based strategies combined with simple digital media to improve biology learning effectiveness. Future research may explore its impact on non-cognitive skills such as critical thinking and scientific attitudes. Furthermore, the results of this study provide opportunities for teachers to be more creative in designing teaching materials that are not only informative but also interactive and relevant to students' lives. By utilizing media such as interactive PowerPoint, teachers can present complex biological content in a more engaging and understandable way.

Virgin Coconut Oil (VCO) is a processed coconut product with high economic and health value. VCO production is known to be relatively easy, inexpensive, and capable of producing high-quality oil rich in benefits, including as a traditional medicine and natural ingredient for beauty and health. This community service activity aims to provide students of the Biology Education Study Program at Buton Muslim University with an understanding and practical skills regarding the VCO production process as a form of utilization of local natural resources, especially coconuts. The methods used in this activity include lectures or delivery of theoretical material, interactive discussions on the content and benefits of VCO, and direct practice in making virgin coconut oil using the settling method. This method was chosen because it does not require complicated equipment, does not use excessive heating, and is able to maintain the natural quality of the resulting oil. During the implementation, students are invited to observe and follow the entire process, from selecting good coconut raw materials, the grating process, squeezing coconut milk, natural fermentation through settling, to the process of separating oil from sediment and filtering. The results of this activity demonstrated that students not only understood the concept and theory of VCO production but also produced 1,500 ml of virgin coconut oil packaged in five 300 ml bottles. The VCO was then sold to the public for approximately Rp 30,000 per bottle. Through this activity, students not only gained contextual learning experiences but also developed insights into sustainable, locally-based entrepreneurship.

The research conducted in class X IPA SMA Negeri 1 Rengat aims to determine the level of student learning difficulties in biology subjects in class X IPA SMA Negeri 1 Rengat. This study used a cluster sampling sample with a population of 90 students of class X IPA. Data analysis in this study used percentage techniques. The results of the study showed that the level of learning difficulties of students at SMA Negeri 1 Rengat, physiological indicators were 56.4% in the sufficient category, psychological indicators were 65.26% in the sufficient category, family indicators were 66.01% in the sufficient category, school indicators were 59.46% in the sufficient category, community indicators were 62.5% in the sufficient category, the average overall percentage was 61.92% in the good enough category.

Biology education requires a learning strategy that can improve conceptual understanding while developing students' critical thinking skills. This study aims to analyze the influence of the Project-Based Learning (PjBL) learning model on the learning outcomes of class X students on biodiversity materials at SMA Negeri 1 Melonguane. The research uses a quasi-experimental method with a Pretest-Posttest Control Group Design, involving two classes, namely the X-A experimental class taught using the PjBL model with a mini ecosystem project, and the X-D control class. The control class is taught conventionally. The analysis results showed a significant increase in the posttest score of the experimental class with an average of 79.33 compared to the control class with an average of 73.39. These findings indicate that applying the PjBL model effectively creates active learning, increases student engagement, and develops problem-solving skills and creativity. The lower variability of scores in the experimental class showed that PjBL could accommodate differences in students' abilities. Project-based learning also arouses students' motivation and confidence, making Biology learning more contextual and meaningful. The implications of this study show that applying the PjBL model can be an alternative to Biology learning strategies to improve learning outcomes while supporting the development of 21st century skills. This research enriches the literature on Biology learning innovations. It opens opportunities for further studies related to the application of PjBL to other materials or long-term impact analysis on students' academic achievements.

This study aims to identify the presence of Staphylococcus aureus in fermented pedo fish (Decapterus spp.) products using the Gram staining technique. The Gram staining technique is a simple but effective method to distinguish Gram-positive and Gram-negative bacteria based on differences in their cell wall structure. Samples were taken from traditional fermented pedo fish and analyzed in a microbiology laboratory. The results showed the presence of Gram-positive coccus-shaped bacteria that clustered like grapes, in accordance with the morphological characteristics of S. aureus. This identification can be used as an initial step in evaluating the microbiological quality of fermented products.