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Background: Acne vulgaris is one of the most common skin problems and is closely associated with the activity of Propionibacterium acnes. Long-term use of antibiotics may lead to bacterial resistance; therefore, alternative antibacterial agents derived from natural products are needed. White rice (Oryza sativa L.) is known to contain bioactive compounds such as flavonoids, alkaloids, terpenoids, tannins, and saponins, which potentially exhibit antibacterial activity. Objective: This study aimed to evaluate the antibacterial activity of ethanol extract of white rice against the growth of Propionibacterium acnes. Methods: This research was conducted as a laboratory experimental study using a post-test only control group design. The ethanol extract of white rice was prepared by maceration using 70% ethanol. Antibacterial activity was evaluated using the disc diffusion method with extract concentrations of 100%, 50%, 25%, 12.5%, and 6.25%. Clindamycin was used as a positive control, while sterile distilled water served as a negative control. The parameter observed was the diameter of the inhibition zone. Result: The results of phytochemical screening showed that the ethanol extract of white rice (Oryza sativa L.) contains flavonoids, alkaloids, and terpenoids. Antibacterial activity test showed that the ethanol extract of white rice at concentrations of 100%, 50%, and 25% was able to inhibit the growth of Propionibacterium acnes by forming an inhibition zone, while at concentrations of 12.5% and 6.25% no inhibition zone was formed. The highest inhibition zone diameter was found at a concentration of 100%. The ethanol extract of white rice at concentrations of 100% (7.65 mm), 50% (6.77 mm), and 25% (6.15 mm) was able to inhibit the growth of Propionibacterium acnes, and was classified as having moderate inhibition. In contrast, at concentrations of 12.5% and 6.25%, the ethanol extract of white rice did not show any inhibitory activity detected in vitro using the disc diffusion method. The results of statistical analysis showed a p value <0.001, which means there was a significant difference in the diameter of the inhibition zone between the treatment groups. Conclusion: In conclusion, the ethanol extract of white rice exhibits antibacterial activity against Propionibacterium acnes in a concentration-dependent manner; however, its inhibitory effect remains lower than that of clindamycin.

Soursop leaves (Annona muricata L.) are known to contain secondary metabolites such as alkaloids, flavonoids, tannins, saponins, and polyphenols, which have antibacterial potential. This study aimed to formulate soursop leaf extract into a gel dosage form and to evaluate its antibacterial activity against Pseudomonas aeruginosa. This research was conducted as an experimental laboratory study. Soursop leaf extract was obtained using the maceration method with 96% ethanol as the solvent and then formulated into gel preparations with extract concentrations of 5%, 10%, and 15%. Physical evaluation of the gel preparations included organoleptic test, homogeneity, pH, spreadability, and viscosity. Antibacterial activity was evaluated using the well diffusion method on Nutrient Agar medium. The results showed that all gel formulations met the physical requirements for topical preparations. The antibacterial activity test demonstrated that the soursop leaf extract gel inhibited the growth of Pseudomonas aeruginosa, with the 15% concentration producing the largest inhibition zone of 10 mm compared to other concentrations. In conclusion, soursop leaf extract gel has potential to be developed as a topical antibacterial agent against Pseudomonas aeruginosa.

Antibiotic resistance has emerged as a significant global health challenge, prompting the exploration of alternative antimicrobial agents. This study focuses on the synthesis and antibacterial potential of plant-based nanoparticles, specifically silver nanoparticles AgNPs, synthesized using neem leaf extract Azadirachta indica. The research aims to assess the effectiveness of these green-synthesized nanoparticles against Escherichia coli E. coli), a common pathogen responsible for numerous infections, including those resistant to conventional antibiotics. The synthesis of AgNPs was performed using neem leaf extract as a reducing and stabilizing agent, following a green synthesis approach that is environmentally friendly and avoids harmful chemicals. The synthesized nanoparticles were characterized using UV-Vis spectroscopy, Transmission Electron Microscopy TEM, and X-ray Diffraction XRD, ensuring the particles’ size, shape, and crystalline structure were in the desired range. Antibacterial activity was assessed using the agar diffusion method, comparing the inhibition zones formed by the nanoparticles with those of traditional antibiotics. The findings revealed that the silver nanoparticles displayed significant antibacterial activity against E. coli, with inhibition zones comparable to conventional antibiotics, indicating their potential as an effective alternative in combating antibiotic-resistant bacteria. Moreover, these nanoparticles exhibited high stability and biocompatibility, making them a promising candidate for further biomedical applications. The results suggest that neem-based AgNPs could serve as an eco-friendly solution for addressing antibiotic resistance. Future research is recommended to explore the broad-spectrum activity of these nanoparticles against other bacterial pathogens and to assess their safety and efficacy in clinical settings.

Enteric bacterial infectious diseases such as Escherichia coli are still a serious health problem in many countries, mainly due to the increasing cases of antibiotic resistance that cause the effectiveness of conventional therapies to decline. This condition encourages the search for safer and more effective natural alternatives, one of which is propolis produced by the Trigona sp. bee Trigona sp. Propolis is known to contain a variety of bioactive compounds, including flavonoids and phenolic acid, which act as antimicrobials. This study aims to test the antibacterial activity of propolis extract against E. coli by the sumpray diffusion method. The results of the study showed that there was an inhibition zone that varied according to the concentration of the extract. At a concentration of 10%, an average inhibition zone of 19.85 mm (strong category), a 20% concentration of 22.35 mm (very strong), and a concentration of 30% reaches 26.62 mm (very strong). In comparison, the positive control of ciprofloxacin produced 21.25 mm of resistance, while the negative control (DMSO) showed no activity. ANOVA analysis confirmed significant differences between treatments (p < 0.05). These findings confirm that propolis extract has the potential to be developed as an effective natural antimicrobial agent.

Bajakah tampala (Spatholobus littoralis Hassk.) is a medicinal plant that is traditionally used by the Dayak people to overcome various diseases, including indigestion such as diarrhea and dysentery. This plant is known to contain secondary metabolites, including flavonoids, tannins, saponins, alkaloids, and phenolics, which have the potential to have antibacterial activity. This study aims to evaluate the antibacterial activity of pulp extract of patchy bajakah with n-hexane solvent against Escherichia coli ATCC 25922. The extract was obtained through the maceration method and tested using the well diffusion method at concentrations of 10%, 20%, and 30%. The results showed a relatively low yield of the extract (0.10–0.14%) with a reddish-orange color. The inhibition zones formed ranged from 4.28–5.55 mm with significant differences between concentrations (p = 0.000), but the antibacterial activity was relatively low. These findings indicate that the active compounds of the patch stalk are likely to be more polar or semi-polar, so they are less optimally extracted using the nonpolar solvent n-hexane. Therefore, further research with different solvent variations, particularly those that are polar, is urgently needed to uncover the greater antibacterial potential of these plants.

Skin that is prone to bacterial infections requires proper care using products containing antibacterial agents. One potential alternative is kaffir lime leaves (Citrus hystrix), which are known to contain antibacterial compounds. This study aimed to evaluate the formulation of liquid soap containing kaffir lime leaf extract and to determine the optimal concentration for inhibiting bacterial growth. An experimental laboratory design was applied, and the antibacterial effectiveness was tested using the disk diffusion method against two test bacteria, namely Staphylococcus aureus and Escherichia coli. Statistical analysis was performed using One-Way ANOVA, followed by Scheffe’s post-hoc test to identify significant differences among treatment groups. The results indicated that the liquid soap containing kaffir lime leaf extract exhibited good physical quality and met standard parameters, including organoleptic evaluation, pH, viscosity, and foam height tests. Antibacterial testing showed that the highest inhibition zone against Staphylococcus aureus was observed at a 15% concentration with a zone diameter of 18.28 mm (strong category), while the highest inhibition zone against Escherichia coli was also at a 15% concentration with a diameter of 19.03 mm (strong category). The One-Way ANOVA results showed a significance value (p < 0.05), indicating a statistically significant difference among treatments for both bacterial species. These findings suggest that liquid soap formulated with kaffir lime leaf extract, particularly at a 15% concentration, has strong antibacterial activity and potential as an effective skin care product. Further studies are recommended to evaluate safety, stability, and long-term effectiveness.

This study aims to evaluate the antibacterial activity of shrimp paste extract against Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive) using the paper disk method. The shrimp paste was extracted using ethanol as a solvent and tested for its inhibitory effect on bacterial growth through paper disks infused with various concentrations of the extract. The results showed that the shrimp paste extract exhibited antibacterial activity against both bacterial species, with varying inhibition zone diameters depending on the concentration used. Higher antibacterial activity was observed against Staphylococcus aureus compared to Escherichia coli, indicating that shrimp paste has potential as a natural antibacterial agent, particularly against Gram-positive bacteria. These findings support the utilization of fishery by-products such as shrimp paste as an eco-friendly alternative source of natural antibacterial agents.

Shrimp petis is a traditional Indonesian fermented product that is susceptible to contamination by pathogenic bacteria such as Staphylococcus aureus, Escherichia coli, and Pseudomonas spp. Preservation is generally carried out with chemical antibiotics, but their use can cause side effects and microbial resistance. This study aims to examine the effectiveness of ginger extract (Zingiber officinale) as a natural antibiotic against bacteria in shrimp petis. Ginger extract is known to contain active compounds such as gingerol and shogaol which have antibacterial activity. Through literature studies and disc diffusion method analysis, it was found that ginger extract was able to produce an inhibition zone of 12–22 mm against test bacteria. These results indicate that ginger extract is effective in inhibiting the growth of pathogenic bacteria and has the potential to be used as a natural preservative. In addition, a combination of biological, chemical, and natural preservation approaches is recommended to maintain the quality and safety of shrimp petis. The use of ginger extract is in line with the trend of healthy and environmentally friendly food.

White glutinous rice fermentation (tape ketan putih) is a traditional food product containing various microorganisms, including Gram-positive bacteria that may act as contaminants and reduce product quality. Ginger (Zingiber officinale) contains active compounds such as gingerol and shogaol, known for their antibacterial properties. This study aimed to evaluate the antibacterial activity of ginger extract against microorganisms isolated from white fermented glutinous rice. Methods: Ginger extract was obtained by maceration using ethanol as a solvent and tested by disk diffusion method on Nutrient Agar against isolated microorganisms. Results: An inhibition zone was observed around the ginger extract disk, indicating moderate antibacterial activity against Gram-positive bacteria. Conclusion: Ginger (Zingiber officinale) extract shows potential as a natural antibacterial agent against Gram-positive bacteria in tape ketan putih and may be developed as a natural preservative for fermented products.

This study evaluated the antibacterial activity of a combination of microbial compounds from apple cider vinegar (ACV) and keji beling leaf extract against skin and wound pathogenic bacteria, namely Staphylococcus epidermidis (Gram-positive) and Pseudomonas aeruginosa (Gram-negative). ACV is produced through apple fermentation using Saccharomyces cerevisiae and Acetobacter aceti, rich in acetic acid, probiotics, and proteolytic enzymes. Keji beling leaf extract is obtained through ethanol maceration and contains flavonoids, tannins, alkaloids, saponins, terpenoids, and phytosterols—all of which have been shown to be antibacterial and antioxidant. The well diffusion method was used, with four treatments: negative control (DMSO), positive control (chloramphenicol 30µg), keji beling extract, and a combination of ACV + extract in various ratios. The results showed the largest inhibition zone in the 1:1 combination of ACV:extract, respectively 21.2±0.5mm (S. epidermidis) and 18.5±0.6mm (P. aeruginosa), much larger than the extract alone. This finding indicates significant synergism (p<0.05) between ACV and keji beling extract in stopping bacterial growth.

Palm wine (tuak) is a traditional fermented beverage that is prone to microbial contamination, thus requiring natural alternatives with antibacterial properties. This study aimed to determine the effectiveness of Curcuma xanthorrhiza (temulawak) extract in inhibiting microbial growth in tuak samples. The method used was the disc diffusion test with three extract concentrations, 25%, 50%, and 100%. The tuak samples were first femented, serially diluted, and inoculated on Nutrient Agar (NA). Sterile paper discs soaked in each extract were placed on the medium surface and incubated at room temperature for 24 hours. The results showed no visible inhibition zones around the discs at any concentration tested. The conclusion of this study is that temulawak extract at concentrations of 25–100% did not demonstrate antibacterial activity against microorganisms in tuak samples. This study serves as a foundational evaluation of natural antibacterial agents and highlights the need for further optimization of extraction techniques or targeted testing on specific bacterial isolates.

The Sambung Nyawa plant (Gynura procumbens) is known to have various benefits, including as an antibacterial. This study aims to identify and evaluate the antibacterial properties of secondary metabolites from endophytic fungi isolated from Sambung Nyawa leaves against Staphylococcus epidermidis and Staphylococcus aureus bacteria.  Fungi were isolated and then fermented for 7 days using Nutrient Broth media. Antibacterial activity was evaluated using agar diffusion technique, with zone of inhibition as an indicator of activity. The results showed that IFDSN 2 fungal isolate had the highest zone of inhibition, with a very strong category against S. aureus and strong against S. epidermidis. This finding supports the potential of endophytic fungi as an environmentally friendly natural antibacterial source.

The increasing incidence of bacterial resistance to antibiotics has become one of the major challenges in global health, necessitating the exploration of natural antibacterial sources as alternative treatments, such as bioactive compounds derived from sea urchins. This literature review aims to evaluate the antibacterial potential of sea urchin extracts based on existing research findings. The literature search was conducted using the PubMed and Google Scholar databases using the Boolean operators (AND, OR). Inclusion criteria include articles published between 2014 and 2024, available in full text, written in either Indonesian or English, and discussing the antibacterial activity of sea urchin extracts from the species Diadema setosum and/or Echinometra mathaei. Based on the selection process, eight articles met the inclusion criteria and were included in the review. The results of the literature review indicate that sea urchin extracts exhibit antibacterial activity against gram-negative bacteria, including Escherichia coli, Salmonella typhi, Salmonella typhimurium, Shigella flexneri, Pseudomonas aeruginosa, Aeromonas hydrophila, Acinetobacter sp., Citrobacter freundii, and Klebsiella pneumoniae, as well as gram-positive bacteria, including Propionibacterium acnes, Staphylococcus aureus, Bacillus cereus, Bacillus subtilis, Staphylococcus epidermidis, Streptococcus mutans, and Streptococcus sobrinus. These antibacterial effects are attributed to the presence of secondary metabolites such as alkaloids, steroids, flavonoids, saponins, and phenolics.

The cacao plant (Theobroma cacao L.) has long been used in traditional medicine and has been proven to contain phytochemical compounds such as polyphenols, flavonoids, alkaloids, and tannins, which exhibit pharmacological activities, including antioxidant, anti-inflammatory, and antibacterial properties. This study aims to review the potential development of antibacterial formulations based on cacao extract through a literature review. The method used is a qualitative descriptive analysis of national and international journals published in the last 10 years. The review results indicate that extracts from cacao seeds, fruit peels, and leaves are effective in inhibiting the growth of various pathogenic bacteria, both Gram-positive and Gram-negative. Various formulations such as gels, creams, lotions, soaps, toothpaste, and mouthwash have been developed with promising results. The utilization of the cacao plant as an antibacterial agent in pharmaceutical formulations holds great potential as a natural alternative in the healthcare field while also adding value to cocoa industry by-products.

Gallic Acid (GA) is a polyphenolic compound widely found in plants and has shown potential as a natural antibacterial agent. This literature review aims to assess the effectiveness of gallic acid as an antibacterial based on available experimental studies. The reviewed articles include studies published between 2015 and 2025 that specifically investigated the antibacterial activity of gallic acid against pathogenic bacteria. Various studies have demonstrated that GA, either in its pure form or in combination with other materials such as hydrogels, gold nanoparticles (AuNP-GA), graphene oxide (GO-GA), and natural extracts, exhibits significant antibacterial activity against a wide range of bacteria, including Staphylococcus aureus, Escherichia coli, and other pathogenic bacteria. GA-based formulations also show additional benefits, such as promoting wound healing, supporting osteogenesis, and even exerting cytotoxic effects on certain cancer cells. Nevertheless, further research is needed to optimize the clinical application of gallic acid by considering both its safety and efficacy.

Leunca leaves are rich in bioactive compounds such as tannins, alkaloids, flavonoids, phenols and steroids, making it possible to use them as an antibacterial. This community service aims to empower the community by introducing and involving them in the production of herbal emulgel from Leunca leaf extract (Solanum nigrum), which had potential as a natural antibacterial agent, particularly for skin care. Leunca leaves, rich in bioactive compounds such as alkaloids and flavonoids, had antibacterial properties that can effectively treat skin infections. Through counseling and practical training, the community is taught how to process Leunca leaves into an emulgel formulation that is easy to use and has health benefits. The method of this community service was carried out through presentations, distribution of modules, and demonstrations of emulgel production to the community. Evaluation results showed an increase in community knowledge about the benefits of Leunca leaves as a natural antibacterial agent, with knowledge improving from 15% to 80% after the counseling. It is hoped that this initiative will raise awareness and promote the use of local herbal ingredients in skin care, as well as open up opportunities for herbal-based entrepreneurship that can improve the community's well-being

Nutmeg belongs to the Myristicaceae family which consists of 15 genera and 250 species. Nutmeg has several benefits for body health, namely, Improving brain health, Overcoming digestive problems, Maintaining oral health, Treating insomnia. Escherichia coli is a bacterial name taken from the name of the person who discovered it, Theodor Escherich. This study aims to determine the comparison of antibacterial activity of forest nutmeg pulp, peel and leaf extract (Myristica schifferi Warb) against Escherichia coli.: The research method used is the agar diffusion method. The results of the antibacterial activity test of the flesh and skin of forest nutmeg (Myristica schifferi Warb) against Escherichia coli bacteria with concentrations of 50%, 75% and 100% were declared sensitive to inhibit the growth of Escherichia coli bacteria. Meanwhile, the results of the forest nutmeg leaf test with a concentration of 50% were declared intermediate or medium category to inhibit the growth of Escherichia coli bacteria, for concentrations of 75% and 100% were declared sensitive to inhibit the growth of Escherichia coli bacteria.

Escherichia coli is a bacterium that typically resides as a normal flora in the gastrointestinal tract of humans and animals. However, certain strains of E. coli can cause infections in humans. Therefore, antibiotics are needed to inhibit the growth of these infection-causing bacteria. The increasing cases of antibiotic resistance in E. coli necessitate alternative therapies, including the use of natural substances. In West Kalimantan, one potential source is the Pontianak Orange (Citrus nobilis var. microcarpa). This plant contains secondary metabolites that can be used for medicinal purposes, such as the juice extract. The compounds within these secondary metabolites possess antimicrobial properties that could be used as antibacterials. To determine the antibacterial potential of Pontianak Orange juice (Citrus nobilis var. microcarpa) against Escherichia coli. This study utilized an experimental design featuring a post-test only group design. Phytochemical analysis indicated that Pontianak Orange juice (Citrus nobilis var. microcarpa) contains phenolics (+), alkaloids (+), and terpenoids (++). Antibacterial activity testing at all concentrations (25%, 50%, 75%, and 100%) showed no inhibition zones. The juice of Pontianak Orange (Citrus nobilis var. microcarpa) did not show antibacterial effects against the growth of Escherichia coli bacteria.

Bacterial growth is one processes carried out through binary fission for its reproduction process. Bad bacteria in their growth process must course be inhibited in several ways, one which is through the consumption several herbal or traditional plants, such as torch ginger and kalanduyung extracts. The dangers cigarette smoke can reduce the health individuals. Cigarette smoke can have an impact on reducing the number of spermatozoa caused by decrease in the diameter seminiferous tubules for 45 days. The purpose writing journal is to analyze antibacterial activity of torch ginger and kalanduyung extracts on bacterial growth and the effect of cigarettes on the quality male mouse sperm. The writing method uses qualitative type. The results and conclusions that bacterial growth can be inhibited by consuming several traditional plants such as torch ginger extract and kalanduyung leaf extract. In inhibiting growth of antibacterial activity, torch ginger extract has quite good effectiveness with concentration value 20%. The value inhibition zone formed is 17.22. The use largest concentration, namely 80%, has an effective inhibition of bacterial growth which is known to have an average inhibition zone value of 14.9 mm. Furthermore, the quality male mouse sperm does not have specific effect on the provision control treatment related to provision amount of cigarette smoke.

The Miana Coleus atropurpereus plant is a unique plant because it has so many varieties. The differences in these varieties can be seen from the very diverse differences in leaf color. Several studies have known about the active antimicrobial compounds of Coleus atropurpereus miana leaves, namely in the form of flavonoids, saponins, steroids, tannins, essential oils, eugenol, polyphenolic compounds, alkaloids, ethyl salicylate, calcium oxalate, rosmarinic acid compounds. In this study, Escherichia coli and Staphylococcus aureus bacteria were used using thick extracts that had been macerated using 96% alcohol. in this study it showed 8 mm at a concentration of 50%, at a concentration of 25% it showed a clear zone of 7 mm and at a concentration of 12.5% ​​it showed 6 mm. It can be concluded that the extract of Coleus atropurpereus miana leaves is weakly toxic to E. coli and not toxic to S. aureus bacteria..