Publication Search

Dental caries is an oral health problem associated with the activity of Streptococcus mutans. Water hyacinth (Eichhornia crassipes) contains bioactive compounds such as flavonoids, tannins, and alkaloids with antibacterial potential. This study aimed to evaluate the effectiveness of water hyacinth extract against the growth of Streptococcus mutans. The research method used was an in vitro experimental study with a post test only group design. A total of 24 samples were divided into four treatment groups with extract concentrations of 6.25%, 12.5%, 25%, and 50%. Antibacterial activity was tested using the disc diffusion method on Mueller Hinton Agar media. Data were analyzed using the Kruskal-Wallis and Mann-Whitney tests. The results showed that water hyacinth extract inhibited the growth of Streptococcus mutans. The largest inhibition zone was observed at 50% concentration, while the smallest was found at 6.25% concentration. Kruskal-Wallis analysis showed significant differences among groups (p<0.05). In conclusion, water hyacinth (Eichhornia crassipes) extract has antibacterial activity against Streptococcus mutans.

Soursop leaves (Annona muricata L.) are known to contain secondary metabolites such as alkaloids, flavonoids, tannins, saponins, and polyphenols, which have antibacterial potential. This study aimed to formulate soursop leaf extract into a gel dosage form and to evaluate its antibacterial activity against Pseudomonas aeruginosa. This research was conducted as an experimental laboratory study. Soursop leaf extract was obtained using the maceration method with 96% ethanol as the solvent and then formulated into gel preparations with extract concentrations of 5%, 10%, and 15%. Physical evaluation of the gel preparations included organoleptic test, homogeneity, pH, spreadability, and viscosity. Antibacterial activity was evaluated using the well diffusion method on Nutrient Agar medium. The results showed that all gel formulations met the physical requirements for topical preparations. The antibacterial activity test demonstrated that the soursop leaf extract gel inhibited the growth of Pseudomonas aeruginosa, with the 15% concentration producing the largest inhibition zone of 10 mm compared to other concentrations. In conclusion, soursop leaf extract gel has potential to be developed as a topical antibacterial agent against Pseudomonas aeruginosa.

Antibiotic resistance has emerged as a significant global health challenge, prompting the exploration of alternative antimicrobial agents. This study focuses on the synthesis and antibacterial potential of plant-based nanoparticles, specifically silver nanoparticles AgNPs, synthesized using neem leaf extract Azadirachta indica. The research aims to assess the effectiveness of these green-synthesized nanoparticles against Escherichia coli E. coli), a common pathogen responsible for numerous infections, including those resistant to conventional antibiotics. The synthesis of AgNPs was performed using neem leaf extract as a reducing and stabilizing agent, following a green synthesis approach that is environmentally friendly and avoids harmful chemicals. The synthesized nanoparticles were characterized using UV-Vis spectroscopy, Transmission Electron Microscopy TEM, and X-ray Diffraction XRD, ensuring the particles’ size, shape, and crystalline structure were in the desired range. Antibacterial activity was assessed using the agar diffusion method, comparing the inhibition zones formed by the nanoparticles with those of traditional antibiotics. The findings revealed that the silver nanoparticles displayed significant antibacterial activity against E. coli, with inhibition zones comparable to conventional antibiotics, indicating their potential as an effective alternative in combating antibiotic-resistant bacteria. Moreover, these nanoparticles exhibited high stability and biocompatibility, making them a promising candidate for further biomedical applications. The results suggest that neem-based AgNPs could serve as an eco-friendly solution for addressing antibiotic resistance. Future research is recommended to explore the broad-spectrum activity of these nanoparticles against other bacterial pathogens and to assess their safety and efficacy in clinical settings.

Pine soda is a natural fermented liquid from pine needles (Pinus merkusii) which has a distinctive aroma and contains bioactive compounds with potential as antimicrobial agents. This study aims to determine the antibacterial activity of pine soda against Staphylococcus aureus using the disc diffusion method and to compare its effectiveness with the antibiotic ampicillin as a positive control. The process of making pine soda is carried out through simple fermentation of Pinus merkusii pine needles under certain conditions to produce a fragrant liquid. Furthermore, qualitative tests are carried out to identify the content of bioactive compounds, such as flavonoids, phenols, and essential oils, which are known to have antibacterial properties. In the antibacterial activity test, paper discs that have been soaked in pine soda solutions with graded concentrations (2%, 4%, 6%, 8%, and 10%) are placed on agar media that have been inoculated with S. aureus. The results of the observation showed the presence of inhibition zones in each treatment with varying sizes. The largest inhibition zone was obtained at a concentration of 10% with a diameter of 10 mm, which is included in the medium inhibition category. As a comparison, ampicillin produced an inhibition zone of 26.3 mm, while distilled water, the negative control, showed no inhibition zone. These findings indicate that the antibacterial activity of pine soda is influenced by its bioactive compound content, although its effectiveness is still lower than that of standard antibiotics. This potential opens up opportunities for developing pine soda as a safe natural antibacterial agent, whether in the form of health products such as antiseptics, natural cleaners, or functional beverages that support body health. Further research is needed to optimize the fermentation process, increase the concentration of active compounds, and test its effectiveness against various types of pathogenic bacteria.

Staphylococcus Aereus is a common us of skin and soft tissue infection. The aim of study was to evaluate the effectivaness of Betadine solution, Sos, and hand sanitizer as antimicrobial agent against S. Aereus. The  study used a laboratory based experimental design, Where S. aereus was exposed to paper discs soaked in betadine, SOS, and hand sanitizer, the betadine disc showed a more significant antimicrobial effect  than other This study shows that betadine is more effective than the others. Testing is carried out using the diffusion method where paper discs that have been soaked in antimicrobial substances are attached to the surface of agar media that has grown bacteria. In this way, a clear zone will be visible surrounding the disc paper as a sign of the microbe's sensitivity to the antimicrobial. 

This research was conducted to assess the antimicrobial activity of Dettol, Betadine, and Harpic against the bacterial growth of Staphylococcus aureus using the disk diffusion technique. The method involved placing sterile paper disks soaked with each test agent onto agar media previously inoculated with S. aureus. Observations revealed that Harpic formed the widest inhibition zone, measuring 15 mm, followed by Dettol with a 12 mm zone, and Betadine with the smallest at 8 mm. Meanwhile, the control disk exhibited no inhibitory effect (0 mm). These results suggest that Harpic has the highest antimicrobial potential among the three products tested in suppressing Staphylococcus aureus growth.

White glutinous rice fermentation (tape ketan putih) is a traditional food product containing various microorganisms, including Gram-positive bacteria that may act as contaminants and reduce product quality. Ginger (Zingiber officinale) contains active compounds such as gingerol and shogaol, known for their antibacterial properties. This study aimed to evaluate the antibacterial activity of ginger extract against microorganisms isolated from white fermented glutinous rice. Methods: Ginger extract was obtained by maceration using ethanol as a solvent and tested by disk diffusion method on Nutrient Agar against isolated microorganisms. Results: An inhibition zone was observed around the ginger extract disk, indicating moderate antibacterial activity against Gram-positive bacteria. Conclusion: Ginger (Zingiber officinale) extract shows potential as a natural antibacterial agent against Gram-positive bacteria in tape ketan putih and may be developed as a natural preservative for fermented products.

Orthosiphon aristatus is a medicinal plant that has the potential as a natural antibacterial agent. This study aims to evaluate the effect of cat's whiskers leaf extract on microbial growth using Nutrient Agar (NA) media with variations in extract concentrations of 100 gr, 50 gr, and 25 gr. Antibacterial activity tests were carried out using the well diffusion paper disk method, and observations of microbial growth inhibition zones were carried out after incubation for 24 hours at 37°C. The test results showed that there was inhibition of microbial growth that varied according to the concentration of the extract used; a concentration of 100 gr gave the largest inhibition zone compared to 50 gr and 25 gr. Data analysis showed that increasing extract concentration was directly proportional to increasing antibacterial effectiveness. These findings indicate that Orthosiphon aristatus leaf extract has the potential as a natural antibacterial that is effective in inhibiting microbial growth in NA media, so it has the potential to be developed as an active ingredient in pharmaceutical formulations.

This study aims to isolate and identify microorganisms in the form of bacteria and fungi in tilapia fish waste and to demonstrate the effectiveness of turmeric extract (Curcuma longa Linn.) as an antimicrobial agent. Tilapia fish waste samples were fermented for seven days and then microorganisms were isolated using Nutrient Agar (NA) media for bacteria and Potato Dextrose Agar (PDA) for fungi. Turmeric extract treatment was carried out using the disc diffusion method. The results showed the presence of several species of Gram-positive and Gram-negative bacteria, as well as fungal colonies with dominant characteristics of Aspergillus sp. and Fusarium sp. Turmeric extract with a concentration of 20% showed a significant inhibition zone against the growth of pathogenic bacteria and fungi. The content of secondary metabolites such as alkaloids, tannins, and phenolics in turmeric extract is thought to be the main factor in antimicrobial activity.

Palm wine (tuak) is a traditional fermented beverage that is prone to microbial contamination, thus requiring natural alternatives with antibacterial properties. This study aimed to determine the effectiveness of Curcuma xanthorrhiza (temulawak) extract in inhibiting microbial growth in tuak samples. The method used was the disc diffusion test with three extract concentrations, 25%, 50%, and 100%. The tuak samples were first femented, serially diluted, and inoculated on Nutrient Agar (NA). Sterile paper discs soaked in each extract were placed on the medium surface and incubated at room temperature for 24 hours. The results showed no visible inhibition zones around the discs at any concentration tested. The conclusion of this study is that temulawak extract at concentrations of 25–100% did not demonstrate antibacterial activity against microorganisms in tuak samples. This study serves as a foundational evaluation of natural antibacterial agents and highlights the need for further optimization of extraction techniques or targeted testing on specific bacterial isolates.

The Sambung Nyawa plant (Gynura procumbens) is known to have various benefits, including as an antibacterial. This study aims to identify and evaluate the antibacterial properties of secondary metabolites from endophytic fungi isolated from Sambung Nyawa leaves against Staphylococcus epidermidis and Staphylococcus aureus bacteria.  Fungi were isolated and then fermented for 7 days using Nutrient Broth media. Antibacterial activity was evaluated using agar diffusion technique, with zone of inhibition as an indicator of activity. The results showed that IFDSN 2 fungal isolate had the highest zone of inhibition, with a very strong category against S. aureus and strong against S. epidermidis. This finding supports the potential of endophytic fungi as an environmentally friendly natural antibacterial source.

Infections and antibiotic resistance pose significant challenges to global healthcare. This study aims to evaluate the antibacterial potential of endophytic fungi isolated from betel leaves (Piper betle L.) against Staphylococcus aureus and Staphylococcus epidermidis. The research involved isolating endophytic fungi using Potato Dextrose Agar (PDA), purifying the isolates, and testing their antibacterial activity through the disk diffusion method. The results revealed that three endophytic fungal isolates produced inhibition zones, with the highest average diameter of 17.46 mm against Staphylococcus aureus and 18.66 mm against Staphylococcus epidermidis. These findings indicate the potential of betel leaf endophytic fungi as a natural source of antibacterial agents. This study underscores the role of endophytic fungi in developing new antibiotics to combat bacterial resistance.    

This study synthesized novel sulfadiazine-imine derivatives (A1 and A2) using a microwave-assisted method by reacting sulfadiazine with orsellinaldehyde and 2-hydroxy-5-methylbenzaldehyde. The synthesis involved the nucleophilic addition of sulfadiazine to the aldehyde groups, forming imine bonds efficiently under microwave irradiation within 15 minutes. The antibacterial efficacy of the synthesized derivatives was tested against Bacillus subtilis, Streptococcus pneumoniae, and Escherichia coli using agar well diffusion methods. The derivatives A1 and A2 exhibited significantly improved antibacterial activities compared to sulfadiazine, with A1 showing the highest efficacy. Specifically, inhibition zones for Bacillus subtilis reached 26 mm (A1 at 100 mg/mL) compared to 20 mm for sulfadiazine, and similar trends were observed against E. coli and S. pneumoniae. Furthermore, the cytotoxic activity against MCF-7 breast cancer cells revealed that both derivatives demonstrated dose-dependent cytotoxicity. For derivative A1, cell viability decreased to 19% at 320 ppm with an estimated IC50 between 40 and 80 ppm. Derivative A2 showed comparable cytotoxic behavior, with cell viability dropping to 12.6% at the highest concentration tested. The enhanced antibacterial and anticancer properties are attributed to increased lipophilicity and improved cellular penetration conferred by the imine functional group. This research highlights the potential of microwave-assisted derivatization of sulfadiazine to produce compounds with promising therapeutic applications.

Origanum majorana demonstrates a broad spectrum of effectiveness against various diseases, exerting antimicrobial effects against different pathogenic microorganisms. The goals of this research are to provide light on how Origanum majorana extract inhibits human pathogenic bacteria, how it may prevent biofilm development, and how it affects bacterial adhesion. The antibacterial activity of the water-based Origanum majorana extract was assessed in this research using two different methodologies. "Antibiotics were compared to its effectiveness using an agar-well diffusion assay and disc diffusion method," the first step. Additionally, the extract's ability to inhibit biofilm formation and bacterial adherence was assessed through specialized tests. All bacterial isolates of Gram negative, Gram-positive bacterial types were sensitive to Origanum majorana extract and the range of inhibition zone (30 to 24) mm. Although floxacin was effective against some of these isolates, the majority of them were resistant. Most bacterial Gram negative types were exhibit moderate adherence and biofilm activity to this extracts and some bacterial isolated of bacteria were exhibit high adherence and biofilm activity to the watery extracts of Origanum majorana. This research confirms previous findings that Origanum majorana extracts are very effective against a wide variety of clinical isolates of bacteria, including Gram-negative as well as Gram-positive varieties. Notably, the extracts were found to be more effective than commercially available antibiotics. Furthermore, the extracts displayed significant inhibition of bacterial adherence and biofilm formation. Based on these findings, Origanum majorana extracts have great promise as an effective antibacterial and anti-biofilm agent.

Nutmeg belongs to the Myristicaceae family which consists of 15 genera and 250 species. Nutmeg has several benefits for body health, namely, Improving brain health, Overcoming digestive problems, Maintaining oral health, Treating insomnia. Escherichia coli is a bacterial name taken from the name of the person who discovered it, Theodor Escherich. This study aims to determine the comparison of antibacterial activity of forest nutmeg pulp, peel and leaf extract (Myristica schifferi Warb) against Escherichia coli.: The research method used is the agar diffusion method. The results of the antibacterial activity test of the flesh and skin of forest nutmeg (Myristica schifferi Warb) against Escherichia coli bacteria with concentrations of 50%, 75% and 100% were declared sensitive to inhibit the growth of Escherichia coli bacteria. Meanwhile, the results of the forest nutmeg leaf test with a concentration of 50% were declared intermediate or medium category to inhibit the growth of Escherichia coli bacteria, for concentrations of 75% and 100% were declared sensitive to inhibit the growth of Escherichia coli bacteria.

Introduction: Itch Leaves (Laportea decumana) is a medicinal plant that grows widely in eastern Indonesia, especially Maluku. This plant usually grows in humid areas, surrounded by or adjacent to sago trees, and near rivers. Local people usually use the leaves to treat various complaints such as pain, fatigue and joint and muscle pain, or as a muscle relaxant. Objective: of this research is to determine the secondary metabolite content of the ethanol extract of itchy leaves (Laportea decumana) and test the antibacterial activity of the ethanol extract of itchy leaves (Laportea decumana) against the growth of Staphylococcus aureus bacteria. Method: Used in this research was the Agar diffusion method with itchy leaf test material using concentrations of 60%, 65%, 70% and 75%, chloramphenicol as a positive control and distilled water as a negative control. Results: research shows that itchy leaf extract (Laportea decumana) at a concentration of 60% has an inhibition zone diameter of 15.5 mm, at a concentration of 65% the diameter of the inhibition zone is 20.5 mm, at a concentration of 70% it has 25 mm, while at a concentration of 75% it has The highest area of inhibition against Staphylococcus aureus is 29 mm. Itchy leaf extract (Laportea decumana) at a concentration of 75% has a strong inhibitory level against Staphylococcus aureus bacteria, namely with an inhibitory zone diameter of 29 mm. Conclusion: There are secondary metabolite compounds in the ethanol extract of itchy leaves (Laportea decumana) and antibacterial activity against the growth of Staphylococcus aureus bacteria.

Japanese papaya leaves (Cnidoscolus aconitifolius) contain active compounds such as flavonoids, tannins, saponins, alkaloids and terpenoids which have the potential to have antibacterial activity. The aim of this research is to determine the antibacterial activity of Japanese papaya leaves against Staphylococcus aureus bacteria. Extraction of Japanese papaya leaves has been carried out using the maceration method and 96% ethanol solvent. The antibacterial activity test used the agar diffusion method using a cylindrical plate. Sterile distilled water was used as a negative control while as a positive control the antibiotic ciprofloxacin was used. The concentrations of Japanese papaya leaf ethanol extract used in testing were 10%, 20%, and 30%. The research results showed that Japanese papaya leaf ethanol extract with a concentration of 30% had the widest inhibition zone diameter, namely 17.296 mm. Meanwhile, at a concentration of 20%, the average inhibitory zone diameter was 15,222 mm, and at a concentration of 10%, the average inhibitory zone diameter was 13,018 mm. These three concentrations were included in the strong category based on Greenwood classification.  

Jatropha curcas (Jatropha Curcas L) is a plant that has been widely used by the public to treat various diseases and has antibacterial power. This cannot be separated from the secondary metabolite content it contains. The presence of endophytic microbes in these plants makes them interesting to study. This study aimed to determine the number of endophytic fungal isolates and the antibacterial activity of endophytic fungal isolates from Jatropha leaves against Klebsiella pneumoniae and Streptococcus pneumoiae. The test methods of this research are isolation and purification of endophytic fungi and identification of bacteria-producing endophytic fungi of jatropha leaves, as well as testing the diameter of the inhibitory zone of endophytic fungi of jatropha leaves against Klebsiella pneumoniae and Streptococcus pneumoniae using the agar diffusion method using paper disks. The results of the research obtained 9 isolates which were suspected to be Chaetomium sp DJPT 1, Fusarium sp DJPT 2. Gliocladium sp DJPT 3, Aspergillus fumigatus DJPT 4, Geotrichum sp DJPT 5, Aspergillus niger DJPM 1, Cladosporium sp DJPM 2, Beauveria bassiana DJPM 3, and Aspergillus flavus DJPM 466. Endophytic fungal isolates from Jatropha leaves have antibacterial activity against the growth of Klebsiella pneumoniae and streptococcus pneumoniae.

Squid ink and seaweed have the potential to be utilized as medicines, one of which is as an antibacterial medicinal material. because it contains secondary metabolite compounds that can inhibit the growth of Streptococcus Mutans bacteria. This type of research is a laboratory experiment with the method used, namely maceration for phytochemical screening tests and agar diffusion for antibacterial effectiveness tests with concentrations used in squid ink (Loligo sp) namely 5%, 10%, 15% 20% and in seaweed (Eucheuma cottonii) 1%, 5%, 10%, 15%. The results of the research conducted on phytochemical screening tests contained secondary metabolite compounds in squid ink (Loligo sp) triterpenoids, steroids and seaweed (Eucheuma cottonii) alkaloids, flavonoids, hodroquinone phenols, and tannins. From the research conducted on the antibacterial effectiveness test of squid ink (Loligo sp) and seaweed (Eucheuma cottonii) extracts against Streptococcus mutans bacteria, namely in both samples have the effectiveness in inhibiting Streptococcus mutans bacteria, with a high inhibition of squid ink concentration of 20% by 20.5 mm, and in seaweed concentration of 15% by 23 mm. The analysis used is spss using two tests, namely the shapiro wilk test and the mann whitney test. The conclusion of the mann-whitney test results with a p-value = 0.663 indicates there is no significant difference between squid ink extract (Loligo sp) and seaweed extract (Eucheuma cottonii).

This study was conducted to determine the antimicrobial effectiveness of chemical compounds from the water fraction of leunca fruit. Leunca fruit extract was obtained by maceration, then the results of maceration were evaporated and then fractionated. Antimicrobial activity testing was carried out using the agar diffusion method against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922 and Candida albicans ATCC 01231. The concentration variations of leunca fruit water fraction used were 10%, 20%, 30%, 40%, and 50%. The results of the antimicrobial activity test of leunca fruit water fraction compounds obtained the average inhibition diameter of Staphylococcus aureus ATCC 25923 microbes respectively 7.5 mm; 8.3 mm; 8.8 mm; 9.5 mm; and 10.4 mm. On the microbe Escherichia coli ATCC 25922 consecutively 7.8 mm; 8.1 mm; 9.3 mm; 10 mm; and 11.4 mm. while on the microbe Candida albicans ATCC 01231 the diameter of the bland chemical compound of leunca fruit water fraction is only found in the 50% concentration variation with an average inhibition diameter of 9.1 mm. Based on the results obtained, it can be concluded that the water fraction of leunca fruit (Solanum Nigrum L.) has activity as an antimicrobial against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922 and Candida albicans ATCC 01231 with the largest inhibition zone diameter shown in the 50% concentration variation.