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CBT Room 2 at the Faculty of Medicine, Universitas Muhammadiyah Purwokerto, is used for computer-based lectures and practicums, which may lead to exposure to pathogenic microorganisms from device surfaces, such as computer mice. This study aims to identify pathogenic bacteria and fungi present on mouse surfaces and to evaluate the influence of room temperature and humidity on microbial growth. A total of 60 mouse samples were tested using Nutrient Agar (NA) for bacteria and Sabouraud Dextrose Agar (SDA) for fungi. The recorded room temperature was 25.6°C with a humidity level of 74%. The identification results revealed the presence of Bacillus sp., Escherichia coli, Staphylococcus sp., and Streptococcus sp. on NA media, with Gram-positive bacteria dominating (78.33%). Meanwhile, Aspergillus sp. (40.00%) and Penicillium sp. (38.33%) were found on SDA media. These findings indicate that, although the room's temperature and humidity are not at optimal levels, they still support the growth of mesophilic microbes. The implications suggest the need for routine sterilization efforts to prevent cross-infection risks in the learning environment.  

This study aims to identify the microscopic structure of fungi growing in Manihot esculenta (cassava) waste using Gram A (crystal violet) staining. Cassava waste is a type of organic waste that has the potential to serve as a growth medium for various microorganisms, including fungi. Microscopic observations were conducted to determine the morphology of fungal cells that grow naturally in the waste. The Gram staining method was used to facilitate visualization of the fungal cell wall structure, with an emphasis on crystal violet staining as the primary dye. Waste samples were fermented for 3–5 days to allow fungal growth, then taken and microscopic slides were made. The results showed the presence of purplish-purple fungal hyphae and spores, indicating that the fungal cells were able to retain the crystal violet stain. This structure indicates that the type of fungus growing has a thick cell wall and is likely included in the Ascomycota or Zygomycota group. This identification demonstrates the potential use of Manihot esculenta waste as a substrate for microbiological studies and the development of fungal-based biotechnology. This study also emphasizes the importance of simple staining techniques in assisting the initial identification of microorganisms in organic media.

Pekasam is a traditional fermented fish product that involves microbial activity, particularly lactic acid bacteria, during its fermentation process. This study aims to isolate and identify bacterial species involved in the fermentation of gabus fish (Channa striata). Samples were collected at different stages of fermentation and analyzed microbiologically. The isolation procedure utilized selective media such as MRS agar and Nutrient Agar (NA). The resulting colonies were observed based on morphology and further identified through biochemical tests, Gram staining, and oxidase tests. The results indicated the dominance of several bacterial isolates, primarily from the Lactobacillus genus. Identification confirmed that lactic acid bacteria played a crucial role in the fermentation process and contributed to the characteristic flavor of the pekasam. This research contributes to the scientific understanding of traditional fish fermentation and offers potential for utilizing local probiotic strains in traditional food products.

Pekasam fermentation is still carried out traditionally using spontaneous fermentation. Traditional fermentation products have weaknesses such as inconsistent product quality, excessive microbial growth that is difficult to control, and a low initial LAB population that causes spoilage bacteria to grow rapidly before LAB growth. These weaknesses need to be addressed to produce pekasam with good and uniform quality. One way to do this is by making pekasam on a laboratory scale by adding a starter culture of lactic acid bacteria to the fermentation process (Caterina et.al, 2025). Pekasam is made through a spontaneous fermentation process or with the help of starter cultures, namely microorganisms such as lactic acid bacteria (LAB). In traditional production, fermentation occurs naturally due to microorganisms already present in the raw materials. However, spontaneous fermentation often results in less uniform quality due to uncontrolled microbial growth and a low initial LAB population, allowing spoilage microbes to grow first.

Bekasam is a traditional Indonesian fermented food produced through spontaneous fermentation involving microorganisms, particularly lactic acid bacteria (LAB). LAB plays a crucial role in the fermentation process by producing lactic acid, which lowers pH, inhibits pathogenic microbial growth, and enhances flavor and food safety. This study aimed to identify and characterize LAB isolated from fermented snakehead fish (Channa striata) based on Gram staining and colony morphology. The research began with bacterial isolation using NA Nutrient Agar, followed by macroscopic observation of colony characteristics including shape, edge, elevation, color, and size. Subsequently, Gram staining was performed to determine the cell wall structure and microscopic morphology of the bacteria. The results showed that the bacterial isolates from bekasam generally had circular colonies, white to cream in color, smooth surface, and were Gram-positive rod-shaped cells. In conclusion, bekasam from snakehead fish contains dominant lactic acid bacteria with distinctive colony morphology and Gram-positive characteristics, indicating potential as a natural starter culture for food fermentation.

This study aims to identify Lactobacillus sp. bacteria obtained from the fermentation of Channa striata fish (snakehead fish) originating from Bathin VIII, Sarolangun, Jambi. Snakehead fish is known to have high protein content and bioactive compounds that support the growth of probiotic microorganisms, especially lactic acid bacteria (LAB). Fermentation was carried out naturally for several days under controlled conditions, then isolation and characterization of bacteria were carried out through culture techniques on de Man Rogosa Sharpe Agar (MRSA) media. Initial identification was carried out using colony morphology tests, Gram staining, and basic biochemical tests. The isolation results showed the presence of round, convex, and milky white colonies, and showed Gram-positive and rod-shaped properties, which are typical characteristics of Lactobacillus sp. This study indicates that fermented snakehead fish can be a natural source of Lactobacillus sp. isolates that have the potential to be developed as probiotics in functional foods or local fermented products. These findings are expected to contribute to the development of local microorganism-based food technology and increase the economic value of snakehead fish in the Jambi region. Further research is needed to test the functional potential and safety of the isolated strains.

The potention of a village can be seen in the form of a map when the map comes with a description of the land resources and human resources as the backbone of the rural economy. Land resources can be reflected in the distribution of land use in the village, while the human resources can be illustrated by the small industries or existing SMEs. Map existing describes village areas and sometimes the picture were draw by a person who know the areas. Village map usually contains location or the location of the village facilities, roads, rivers and boundaries hamlet/ RW or village limits. Moreover village map always displays information surrounding neighboring villages. Deficiencies that exist in a map of the village is likely to occur because of the knowledge map-making has not been fully understood by the individual who made the map. It could provide wrong and not information accurate for people whose using the village map. Purpose of this activity is to provide knowledge about the knowledge map to participants. While the purpose of the activities of Community Services were to provide knowledge about the map and the identification of potential rural village in the manufacture of Potential Map. The implementation of the Community Services with interactive discussions by Focus Group Discussion. FGDs were conducted as a way to confirm the village boundaries, facilities and potential village. Community Services activities result were that participants have understood the potential of the village and the knowledge base map. In addition it has arranged a map of Potential Rural Dusun Pisangan, Kelurahan Sumberejo, Kecamatan Tempel, Kabupaten Sleman. The map has been handed over to the village and their representatives - each elementary school in the two villages. 

The development of built-up land in Tanjungpinang City has demonstrated rapid spatial dynamics, in line with the increasing intensity of urban activities since its designation as the capital of Riau Islands Province in 2002. This study aims to analyze the changes in built-up land in Tanjungpinang City over the period 2003 to 2023 and to identify the key factors driving this transformation. The research employed spatial analysis based on Geographic Information Systems (GIS), utilizing satellite imagery maps from the years 2003, 2013, and 2023. The findings indicate that built-up land expanded from 1,133.91 hectares (8%) in 2003 to 3,848.67 hectares (26%) in 2023, with the predominant direction of expansion occurring toward the eastern and southern parts of the city. The primary factors contributing to this growth include population increase, the development of new activity centers such as Pulau Dompak, and the construction of major infrastructure, notably the development of DI Panjaitan streets. These results suggest a growing tendency toward urban sprawl in the physical development of Tanjungpinang City, thus requiring adaptive and sustainable spatial planning strategies.

Black pudding leaf plant (Graptophyllum pictum L.) is a wild plant that is often found in rural areas or intentionally planted as a medicinal plant that has chemical content such as alkaloids, saponins, tannins, flavonoids and steroids which are known to help relieve inflammation. Black leaves have chemical content as anti-inflammatory, diuretic, accelerate the cooking of boils, mild laxative, soften the skin of the feet, soften the stool and deflate the stool. Therefore, this community service aims to provide knowledge to the community about the important properties of the Black pudding leaf plant (Graptophyllum pictum). The study was conducted at Adiwangsa University, Jambi. The research method used was an experimental study consisting of two factors, namely the type of extract and the concentration of the extract. This activity is expected to provide benefits to the community in increasing knowledge and understanding of the important properties of the black pudding plant (Graptophyllum pictum).

Opportunistic fungal infections caused by Candida albicans pose increasing global health challenges, especially in immunocompromised patients. Resistance to conventional antifungal agents such as azoles and echinocandins has driven the search for alternative natural sources of antifungal compounds. This study reviews the isolation and identification of endophytic bacteria from Curcuma xanthorrhiza rhizomes and their potential to produce antifungal metabolites against C. albicans. Literature from the past ten years was analyzed, focusing on bacterial endophytes isolated from temulawak, their identification methods, and antifungal activities. Dominant endophytes such as Bacillus subtilis, B. amyloliquefaciens, and Pseudomonas fluorescens demonstrated significant inhibition of C. albicans growth, with inhibition zones ranging from 10 to 25 mm and minimum inhibitory concentrations (MIC) between 62.5 and 250 µg/mL. Antifungal activity is attributed to bioactive secondary metabolites, particularly lipopeptides produced by Bacillus species. Molecular identification via 16S rRNA sequencing confirms bacterial taxonomy, supporting further biotechnological exploration. This review highlights the promising potential of temulawak endophytic bacteria as a natural source for new antifungal agents to address drug resistance issues.

This study aims to isolate and identify microorganisms in the form of bacteria and fungi in tilapia fish waste and to demonstrate the effectiveness of turmeric extract (Curcuma longa Linn.) as an antimicrobial agent. Tilapia fish waste samples were fermented for seven days and then microorganisms were isolated using Nutrient Agar (NA) media for bacteria and Potato Dextrose Agar (PDA) for fungi. Turmeric extract treatment was carried out using the disc diffusion method. The results showed the presence of several species of Gram-positive and Gram-negative bacteria, as well as fungal colonies with dominant characteristics of Aspergillus sp. and Fusarium sp. Turmeric extract with a concentration of 20% showed a significant inhibition zone against the growth of pathogenic bacteria and fungi. The content of secondary metabolites such as alkaloids, tannins, and phenolics in turmeric extract is thought to be the main factor in antimicrobial activity.

This study aims to identify the presence of Staphylococcus aureus in fermented pedo fish (Decapterus spp.) products using the Gram staining technique. The Gram staining technique is a simple but effective method to distinguish Gram-positive and Gram-negative bacteria based on differences in their cell wall structure. Samples were taken from traditional fermented pedo fish and analyzed in a microbiology laboratory. The results showed the presence of Gram-positive coccus-shaped bacteria that clustered like grapes, in accordance with the morphological characteristics of S. aureus. This identification can be used as an initial step in evaluating the microbiological quality of fermented products.

This study aims to analyze hazard identification in order to improve operational safety aspects in the service road area of I Gusti Ngurah Rai International Airport, Bali. This area is a vital part of the airside that has a high level of activity and complex safety risks, thus requiring special attention to potential hazards that may arise. The research method used is a qualitative descriptive approach, with data collection techniques through direct observation in the field, in-depth interviews with related personnel, and documentation studies relevant to the airport's safety system and operational procedures. The results of the study indicate that there are still significant potential safety risks in the service road area. Some key findings include haphazard parking practices by Ground Support Equipment (GSE), undisciplined behavior of ground handling personnel, and passenger movement in the operational area without adequate officer supervision. These problems are largely caused by weak supervision from the Apron Movement Control (AMC) unit, as well as the low level of understanding and implementation of safety culture among field workers. Through the process of identifying and assessing risks using the Hazard Identification and Risk Assessment (HIRA) approach, researchers succeeded in mapping the severity of risks and developing appropriate mitigation measures, such as regulating GSE parking procedures, improving safety training, and adding supervisory personnel at vulnerable points. It is hoped that the findings of this study can be a basis for consideration for the management of I Gusti Ngurah Rai International Airport, Bali, in formulating policies and strategies for improving operational safety as a whole, especially in implementing a more effective and sustainable Safety Management Sistem (SMS).  

Malaria remains a significant public health issue in Indonesia, particularly in endemic areas such as Keerom Regency, Papua. Efforts to control this disease face challenges due to Plasmodium resistance to synthetic drugs, highlighting the need for safer and more effective alternative treatments. This study aims to identify the secondary metabolite content of several local plants traditionally used as antimalarial remedies. An experimental laboratory study was conducted using phytochemical screening methods. The plants examined included turmeric rhizome (Curcuma longa), meniran leaves (Phyllanthus niruri), papaya leaves (Carica papaya), sambiloto (Andrographis paniculata), and brotowali (Tinospora crispa). Extraction was carried out using the maceration method with 96% ethanol as the solvent. The results showed that all samples contained secondary metabolites such as flavonoids, alkaloids, tannins, and saponins, which are known for their antiplasmodial activity. Extract yields ranged from 18% to 24%, with turmeric rhizome showing the highest yield. These findings suggest that local plants from Keerom Regency hold potential as raw materials for the development of herbal antimalarial drugs. This study serves as a preliminary step for further in vitro and in vivo bioactivity testing.

Occupational diseases continue to pose significant challenges to worker health and productivity worldwide. This integrative literature review analyzes ten selected journal articles from 2019 to 2025 that discuss the role of early detection and workplace health interventions in preventing occupational diseases. Findings reveal that medical surveillance, routine check-ups, and AI-based stress detection technologies are effective tools for identifying health risks at an early stage. Workplace interventions such as ergonomic programs, chemical exposure controls, and community-based health promotion show measurable improvements in worker health and compliance with safety protocols. The Total Worker Health® framework by NIOSH emerges as a comprehensive strategy to integrate health protection and promotion in the workplace. However, implementation gaps remain, particularly in the informal sector, due to limited infrastructure and low awareness. This study concludes that combining early detection with structured health interventions, supported by strong policy and stakeholder involvement, is key to reducing occupational disease burdens.

This study identifies the role of shamans in local wisdom-based wellness tourism in Belumbang Tourism Village, Kerambitan Sub-district, Tabanan Regency. Shamans as traditional spiritual figures play an important role in wellness practices that combine physical healing, spiritual rituals and local ecological understanding. Using observation and interview methods, this research was analyzed based on the six dimensions of wellness in the Extended Wellness Model (Mueller & Kaufmann, 2001), and supported by data from the Global Wellness Institute (Yeung & Johnston, 2018) and the authors' perceptions of shamans. The results show that shaman practices support all dimensions of wellness, especially the spiritual, physical and environmental aspects. Travelers gain benefits such as traditional massages, herbal concoctions, and inner peace through rituals that touch on emotional and mental aspects. Shamans also play a role in educating tourists about the harmony of humans and nature through the responsible use of resources. Thus, shamans not only function as healers, but also as guardians of local culture and tourist attractions that strengthen village identity and support sustainable health and culture-based tourism.

This study aims to examine the extent to which the identification doctrine is applied in establishing corporate criminal liability for acts of corruption. The increasing number of corruption cases involving corporate entities poses a challenge for the criminal justice system in attributing legal fault to legal persons that lack independent intent or will. The objective of this research is to analyze how the identification doctrine is utilized to bridge this issue and to assess its effectiveness and fairness in judicial practice. This study employs a normative legal approach using qualitative methods through the analysis of statutory regulations, legal doctrines, and court decisions. The findings indicate that although the identification doctrine provides a logical foundation for prosecuting corporations through the actions of their directors or executives, its application still faces challenges in distinguishing between individual actions taken personally and those undertaken on behalf of the corporation. This ambiguity may result in legal uncertainty and injustice in the enforcement of criminal sanctions. Therefore, clear and firm criteria are necessary in determining corporate criminal liability to support a more just and accountable legal system.

Grounding or stranding is a shipping accident where the ship stops due to the lowest part of the ship's side or what is known as the keel touching the bottom of the water because the water depth is insufficient so that the ship cannot move freely. Grounding incidents occur more often in shallow waters or narrow shipping lanes. One of the grounding incidents was experienced by researchers while practicing sailing on the MV. Belik Mas, a container ship. At that time, the MV. Belik Mas ran aground when entering the Kapuas River Pontianak shipping lane. Therefore, this study aims to analyze the causes of the grounding of the MV. Belik Mas ship on the Kapuas River shipping lane and analyze the rescue efforts made. The method used in this study is a qualitative approach with the root cause analysis method which aims to identify the cause of the problem to the root cause. The results of the study showed that the root cause of the grounding of the MV. Belik Mas ship was human error, namely due to the lack of consideration in decision making by the captain and bad weather in the form of strong winds.

High blood triglyceride levels indicate impaired fat metabolism and can trigger atherosclerosis, making hypertriglyceridemia a major risk factor for cardiovascular disease (CVD), metabolic syndrome, and acute pancreatitis. The productive-age population often experiences overnutrition due to unhealthy diets and low physical activity, increasing fat and triglyceride accumulation. Stress may also elevate triglyceride levels, necessitating early screening. The Community Service Program (PKM) at Assisi Church aimed to raise awareness of early dyslipidemia detection through triglyceride screening using Point-of-Care Testing (POCT) and educational counseling. Among 76 participants, 39 (57.35%) had high triglyceride levels, while 29 (42.65%) were normal. These findings highlight the importance of regular check-ups and continuous education to reduce cardiometabolic risks and promote healthier lifestyles.

Cockroaches are one of the insects that act as disease vectors that are often found in houses, buildings, including restaurants or eateries. Based on the results of observations conducted at the BTN Kanawa Indah Settlement Block 17, Batu Merah Village, Sirimau District, Ambon City with a dense population and an unclean environment. Viewed according to the habitat and behavior of cockroaches at night, it appears that cockroaches roam around in houses such as kitchens, rooms, toilets, and damp and dirty places. This type of research is descriptive which describes the Density and Identifying the types of cockroaches in the BTN Kanawa Indah Settlement Block, Batu Merah Village, Block 17, Sirimau District, Ambon City. The results of the Cockroach Density study found in the BTN Kanwa Indah Settlement Block 17, Batu Merah Village, Sirimau District, Ambon City were 12 cockroaches, included in the moderate category. Cockroach Identification in BTN Kanawa Indah Settlement Block 17 Batu Merah Village Sirimau District Ambon City there are 14 American cockroaches (Periplaneta americana) at sample point one 2 cockroaches, sample point two 2 cockroaches, and sample point three 5 cockroaches, and point four 3 cockroaches point five 1 cockroach. The survey of cockroach density in BTN Kanawa Indah Settlement Block 17 Batu Mera Village Sirimau District Ambon City, namely the measurement results are in the moderate category. There are 14 American cockroaches (Periplaneta americana), and there are no German cockroaches (Blatella australasiae) obtained from the results of the examination of the 5 sample points, namely positive there is one type of cockroach in the BT Kanawa Indah Settlement Block 17 Batu Merah Village Sirimau District Ambon City.