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Pedada leaves (Sonneratia caseolaris), a type of mangrove plant that grows in tropical and subtropical coastal areas, have high ecological and pharmacological value. In addition to functioning as coastal protection from abrasion and seawater intrusion, this plant is also known to contain various secondary metabolite compounds such as flavonoids, tannins, saponins, and alkaloids. These compounds have been widely reported to have important biological activities, one of which is as an antimicrobial agent. This study aims to identify the effectiveness of pedada leaf ethanol extract in inhibiting the growth of pathogenic microorganisms, especially bacteria that cause infections. The extraction process was carried out using 96% ethanol solvent through the maceration method, namely soaking the material in the solvent for a certain time to optimally obtain active compounds. The obtained extract was then tested for its antimicrobial activity using the disc diffusion method against test bacteria, both Gram-positive and Gram-negative. The inhibition zone formed around the disc was measured as an indicator of the antibacterial effectiveness of the extract. The results showed that pedada leaf extract was able to produce inhibition zones with varying diameters depending on the concentration used. The higher the extract concentration, the larger the diameter of the inhibition zone formed. This indicates a positive relationship between extract concentration and its antibacterial strength. This activity is believed to originate from the ability of compounds such as flavonoids and tannins to damage bacterial cell walls or disrupt microbial metabolic processes. This study provides initial evidence that pedada leaves have potential as an environmentally friendly and sustainable source of natural antibacterials. This potential is highly relevant in efforts to develop alternative antibacterial materials based on local plants, especially amidst increasing bacterial resistance to synthetic antibiotics.

White glutinous rice fermentation (tape ketan putih) is a traditional food product containing various microorganisms, including Gram-positive bacteria that may act as contaminants and reduce product quality. Ginger (Zingiber officinale) contains active compounds such as gingerol and shogaol, known for their antibacterial properties. This study aimed to evaluate the antibacterial activity of ginger extract against microorganisms isolated from white fermented glutinous rice. Methods: Ginger extract was obtained by maceration using ethanol as a solvent and tested by disk diffusion method on Nutrient Agar against isolated microorganisms. Results: An inhibition zone was observed around the ginger extract disk, indicating moderate antibacterial activity against Gram-positive bacteria. Conclusion: Ginger (Zingiber officinale) extract shows potential as a natural antibacterial agent against Gram-positive bacteria in tape ketan putih and may be developed as a natural preservative for fermented products.

This study evaluated the antibacterial activity of a combination of microbial compounds from apple cider vinegar (ACV) and keji beling leaf extract against skin and wound pathogenic bacteria, namely Staphylococcus epidermidis (Gram-positive) and Pseudomonas aeruginosa (Gram-negative). ACV is produced through apple fermentation using Saccharomyces cerevisiae and Acetobacter aceti, rich in acetic acid, probiotics, and proteolytic enzymes. Keji beling leaf extract is obtained through ethanol maceration and contains flavonoids, tannins, alkaloids, saponins, terpenoids, and phytosterols—all of which have been shown to be antibacterial and antioxidant. The well diffusion method was used, with four treatments: negative control (DMSO), positive control (chloramphenicol 30µg), keji beling extract, and a combination of ACV + extract in various ratios. The results showed the largest inhibition zone in the 1:1 combination of ACV:extract, respectively 21.2±0.5mm (S. epidermidis) and 18.5±0.6mm (P. aeruginosa), much larger than the extract alone. This finding indicates significant synergism (p<0.05) between ACV and keji beling extract in stopping bacterial growth.

This study aims to determine the phytochemical content of Black Soldier Fly (BSF) larvae extract, also known as Hermitia illucens. The maceration method with 96% ethanol solvent was used for extraction, followed by evaporation using a rotation evaporator. Qualitative phytochemical tests were carried out to identify compounds such as alkaloids, flavonoids, saponins, terpenoids, polyphenols, steroids, and triterpenoids. The results showed that BSF maggot extract contained alkaloid and flavonoid compounds that were positively detected, while other compounds were not detected by the method used. The alkaloid and flavonoid content has antimicrobial, anti-inflammatory, and antioxidant properties. These results indicate that BSF larvae can function as a source of natural biopharmaceutical active ingredients. However, the type of solvent used and the process conditions used affect the success of the extraction.

Malaria remains a significant public health issue in Indonesia, particularly in endemic areas such as Keerom Regency, Papua. Efforts to control this disease face challenges due to Plasmodium resistance to synthetic drugs, highlighting the need for safer and more effective alternative treatments. This study aims to identify the secondary metabolite content of several local plants traditionally used as antimalarial remedies. An experimental laboratory study was conducted using phytochemical screening methods. The plants examined included turmeric rhizome (Curcuma longa), meniran leaves (Phyllanthus niruri), papaya leaves (Carica papaya), sambiloto (Andrographis paniculata), and brotowali (Tinospora crispa). Extraction was carried out using the maceration method with 96% ethanol as the solvent. The results showed that all samples contained secondary metabolites such as flavonoids, alkaloids, tannins, and saponins, which are known for their antiplasmodial activity. Extract yields ranged from 18% to 24%, with turmeric rhizome showing the highest yield. These findings suggest that local plants from Keerom Regency hold potential as raw materials for the development of herbal antimalarial drugs. This study serves as a preliminary step for further in vitro and in vivo bioactivity testing.

Background: Chinese betel (Peperomia pellucida L.) is an herbal plant rich in bioactive compounds such as flavonoids, alkaloids, and tannins that have the potential to be antioxidants, antimicrobials, and antidiabetics. However, the stability and absorption of its active compounds in the body is still a challenge. Microencapsulation technology and  a floating drug delivery sistem (FDDS) can improve the effectiveness and stability of herbal preparations. Objective: This study aims to formulate and evaluate floating microencapsulation preparations containing Chinese betel herb ethanol extract using modern drug delivery technology. Methods: Ethanol extracts were obtained by maceration method and formulated by ionic gelation technique using a combination of sodium alginate and chitosan at three concentration variations (0.25%, 0.5%, and 0.75%). The evaluation included morphological analysis with SEM, ex vivo buoyancy test on the stomach of Wistar rats, determination of moisture content, and physical stability test using the cycling test method. Results: Microencapsulation with a concentration of 0.75% showed the most compact and delicate morphology, the lowest moisture content (0.64%), and the best physical stability. All formulations are capable of floating, with the longest buoyancy time at a concentration of 0.25% for 8 hours. There were no significant physical changes after six cycles of extreme temperatures. Conclusion: Microencapsulation preparations of Chinese betel ethanol extract in a natural polymer-based floating system show good stability and potential as an innovative herbal formulation to extend retention time in the stomach.    

Sacha inchi (Plukenetia volubilis Linneo) is a plant from the Euphorbiaceae family known for its green star-shaped fruit and brown seeds. This plant has high nutritional value and significant antioxidant activity (Nuha and Sriwidodo 2022). This study aims to identify secondary metabolite compounds and determine the total phenolic content of the methanol extract of Plukenetia volubilis Linneo leaves. The samples were extracted using the maceration method, then phytochemical tests and total phenolic content analysis were carried out using the Folin-Ciocalteu method (Sari et al. 2021). The results of the phytochemical test showed that the methanol extract of sacha inchi leaves contained secondary metabolite compounds such as alkaloids, flavonoids, saponins, and tannins. The total phenolic content obtained from the extract was 19.94%, which indicates the potential for high antioxidant activity. The results of this study indicate that sacha inchi leaves have the potential to be developed as a source of natural bioactive compounds that are beneficial to health.

This study aimed to identify the secondary metabolite compounds present in the ethanol extract of Plukenetia volubilis L. (sacha inchi) shells through phytochemical screening and LC-MS analysis. The extraction was performed using the maceration method with 95% ethanol as solvent, resulting in a yield of 47.33%. Qualitative phytochemical tests revealed the presence of alkaloids, flavonoids, tannins, terpenoids, saponins, and glycosides in the extract. Further LC-MS analysis tentatively identified several phenolic and flavonoid compounds with known biological activities, including sinapinic acid, 1-o-sinapoylglucose, and azelaic acid from the dicarboxylic acid group. The presence of these compounds highlights the potential of sacha inchi shell extract as a natural antioxidant and antidiabetic agent. These findings support the valorization of sacha inchi agricultural waste as a promising raw material for pharmaceutical and nutraceutical applications.

Indonesia is rich in biodiversity, there are around 40,000 species of plants, and they have different uses,such as traditional medicine, can be made into handicrafts, used as decorations, used as natural dyes. Butterflypea flowers have the potential as a natural dye because the anthocyanins contained in their petals emit a bluecolor. The purpose of this study was to determine the anthocyanin content in butterfly pea flowers using themaceration extraction method which is commonly used to determine the best anthocyanin concentration. Thisstudy used a completely randomized factorial design (CRD), namely drying time, (fresh, 1, 2 days) andmaceration temperature (50, 60, 70oC), so that 9 combinations were obtained and each treatment was repeatedtwice. The results of this study indicate that the longer the drying time and the higher the macerationtemperature, the higher the levels of anthocyanin, total phenol, and the darker the color. The researchconducted showed that the best treatment combination was the drying time of 2 days with a macerationtemperature of 70°C to produce butterfly pea flower extract which had antioxidant activity of 54.66%, totalphenol 4.16 KTF (mgGAE/g), anthocyanin 123.48 mg/g, pH 6.03. Color sensory test analysis was 4.5 andwater content of butterfly pea flower was 10.79%. The optimal drying time and maceration temperature willproduce high total phenol and anthocyanin levels. Butterfly pea flowers have high levels of anthocyanins, sothey have the potential to be studied further. 

Cottage salak (Salacca zalacca (Gaertn.) Voss) is one of the tropical fruits that not only has high nutritional value, but also contains bioactive compounds that have the potential to be natural antibacterial. The skin of the salak pondoh fruit is often considered waste, even though this part is rich in chemical compounds such as flavonoids, saponins, phenols, tannins, alkaloids, chlorogenic acid, ferrulic acid, and protocatetic acid. These compounds are known to have biological activity that is able to inhibit the growth of pathogenic microorganisms. Escherichia coli and Salmonella typhi bacteria are the main causes of diarrhea and typhoid fever that affect many people, especially in areas with poor sanitation. Infection of these two bacteria can cause intestinal tissue damage, dehydration, and even serious complications if not treated immediately. Therefore, efforts to find alternative antibacterial agents from natural ingredients such as salak pondoh bark are important. This study aims to test the effectiveness of ethanol extract of salak pondoh bark against E. coli and S. typhi bacteria. Extraction was carried out by the maceration method using ethanol as a solvent, then continued with testing of antibacterial activity using the disc diffusion method. The concentration of the extract was tested on several variations to determine the minimum concentration capable of providing an optimal buffer zone against both test bacteria. The community service program integrated with this research aims to increase public understanding regarding the potential of salak pondoh skin as a natural antibacterial. Education is carried out through counseling and demonstrations of making simple extracts that can be used to clean household appliances or prevent microbial contamination of foodstuffs. With this approach, it is hoped that the community can use salak pondoh skin waste more productively, while reducing dependence on synthetic chemicals that have the potential to cause side effects.

Waru leaves (Hibiscus tiliaceus) are one of the herbal plants that grow well in Indonesia. Empirically, waru leaves have many benefits for treating flu, accelerating the maturation of boils, tonsillitis, and can be used as a hair fertilizer. This study aims to determine the content of waru leaves (Hibiscus tiliaceus). Waru leaves (Hibiscus tiliaceus) are extracted by maceration with methanol solvent. The extraction results then go through a color reaction compound test process and confirmation test. Testing of color reaction compounds includes steroids, saponins, flavonoids, alkaloids, and tannins. This study aims to analyze the chemical compound content contained in waru leaves (Hibiscus tiliaceus) extracted using methanol solvent. This research method includes maceration extraction and chemical compound screening tests using chemical reagents. The results showed that waru leaves extracted using methanol solvent contain steroids, alkaloids, tannins, and saponins. It can be concluded that the chemical compounds contained in hibiscus leaves (Hibiscus tiliaceus) are relevant to human health.

Pomegranate (Punica granatum L.) is a fruit plant that is easy to grow in almost all climates. The utilization of this plant as a traditional medicine is very varied and all parts of the pomegranate plant (Punica granatum L.) can be used as medicine, one of which is pomegranate leaves. This study aims to determine the content contained in pomegranate leaves. Pomegranate leaves were extracted by maceration with 96% ethanol solvent. The extraction results then go through a color reaction compound test process. Testing of color reaction compounds includes saponins, steroids/terpenoids, flavonoids, alkaloids, and tannins. This study aims to analyze the content of chemical compounds contained in pomegranate leaves extracted using 96% ethanol. This research method includes maceration extraction and chemical compound screening tests using chemical reagents. The results showed that pomegranate leaves extracted using 96% ethanol solvent and phytochemical screening showed positive results on alkaloid compounds, steroids/terpenoids, flavanoids, tannins but on saponin metabolites showed negative results.

Milkwood bark (Alstonia scholaris (L.) R. Br.) is one of the medicinal plants of the genus Alstonia. This plant is traditionally used by the community as a medicine for diarrhea, diabetes, malaria, hemorrhoids, and antihypertensives. This plant contains secondary metabolites that have the potential as antibacterial such as alkaloids, flavonoids, saponins and tannins. The purpose of this study was to determine the inhibition of antibacterial activity and the most effective concentration in inhibiting Staphylococcus aureus and Escherichia coli. The sample used in this study was milkwood bark, the sample was extracted by maceration method using 96% ethanol as solvent. The antibacterial testing method used in this study was disc diffusion. The study used a completely randomized design with 9 treatments, namely 1000 ppm, 750 ppm, 500 ppm, 250 ppm, 100 ppm, 50 ppm, 10 ppm, Ciprofloxacin (positive control), sterile distilled water (negative control). The results of the antibacterial activity test at a concentration of 500 ppm showed that milkwood bark extract could inhibit the growth of S. aureus by 8.22 mm and at a concentration of 250 ppm showed that milkwood bark extract could inhibit the growth of E. coli by 8.75 mm. From the test results, it can be concluded that milkwood bark (A. scholaris (L.) R. Br.) has antibacterial activity that has moderate potential in inhibiting S. aureus and E. coli.

Vegetable ingredients are natural sources of active compounds that have the potential to be used as control agents for pathogenic microorganisms in various fields, including health and agriculture. This study aims to evaluate the synergistic effect between aloe vera (Aloe vera L.) and bandotan (Ageratum conyzoides L.) extracts on inhibiting the growth of pathogenic bacteria in different media. Aloe vera extract is obtained using the infundation method, while bandotan extract is obtained using the maceration technique. The concentrations tested were 30%, 45%, and 60% for each extract, as well as a combination of both. Testing was carried out on two main applications: as an antiseptic hand sanitizer by testing germ colonies using Plate Count Agar (PCA), and as a bacteria control Erwinia carotovora, the cause of carrot tuber rot, using the Path Ways Analysis test. The results showed that the combination of aloe vera and bandotan extracts had more significant inhibitory effectiveness than single extracts in both applications. A combination concentration of 60% gave the best results with a reduction in bacterial colonies of up to 90%. The mechanism of action of active compounds such as flavonoids, phenolics and saponins in this vegetable extract plays an important role in damaging bacterial cell membranes and inhibiting the metabolism of microorganisms. This research confirms the potential of using local plant materials as an environmentally friendly and economical solution for controlling pathogenic bacteria in the health and agricultural sectors.

Cassia alata L or generally knowles as Chinese leaves contain phytochemical compounds, one of which is flavonoids which have the potential as antioxidants, antioxidants can be used to prevent diseases with oxidative stress. The purpose of this study was to determine the content of phytochemical screening and qualitative testing of antioxidant activity ethanol ekstract of Chinese leaves (Cassia alata L) using the DPPH method. This study is a qualitative study with an extraction process using the maceration method with 96% ethanol solvent. Phytochemical screening and antioxidant activity testing were carried out by adding specific reagents. The results of phytochemical screening and antioxidant activity testing of Chinese leaves (cassia alata L) ekstract contain bioactive compounds such as flavonoids, saponins, tannins and antioxidant activity testing with DPPH reagents showed that Chinese leaves (cassia alata L) extract has antioxidant activity which is indicated by a change of the color from purple to yellow. From the results obtained, it can be concluded that the flavonoid compound content in Chinese leaves (cassia alata L) extract can potentially act as an antioxidant.

Garlic is a medicinal plant that is widely used for health and is used by people for traditional medicine. Garlic contains various secondary metabolite compounds which have antifungal activity, including tannins, flavonoids, allicin. Flavonoids are the largest group of phenolic compounds. So it is possible that flavonoids also have an effective antifungal effect. This research aims to formulate an ethanol extract of garlic (Allium sativum) ointment with a variety of bases and to determine the physical quality of the ointment. The type of research is laboratory experimental. The method used is maceration of the extract using 1000 ml of 70% ethanol, the design of the ointment formulation is to use three base variations FI (Hydrocarbon Base), FII (Absorption Base), FIII (Water Soluble Base). The results of the research conducted show that ethanol extract of garlic (Allium sativum) can be formulated into an ointment and has stable physical quality. The conclusion from this research is that ethanol extract of garlic (Allium sativum) can be formulated into an ointment and has stable physical quality.

Cashew leaves or also called monkey guava are known by many people, especially in Southeast Sulawesi and are believed to have a number of health benefits but are still rarely known by some people. This study aims to determine the profile of secondary metabolite content and determine the antioxidant activity of ethanol extract of cashew leaves (Anacardium occidentale Linn) using the extraction method by maceration. The extraction process of old cashew leaves is carried out by the maceration method using 96% ethanol solvent for 3 days of soaking and stirring every 24 hours, then concentration is carried out through the evaporation process. Phytochemical testing includes identification of alkaloids, flavonoids, polyphenols, tannins, steroids/triterpenoids and saponins, as well as testing antioxidant activity using the DPPH method (1,1-diphenyl-2-picrylhydrazyl). The results showed that the extract of cashew leaves (Anacardium occidentale Linn) strongly positive contains phenolic compounds, flavonoids, steroids and saponins. Based on the results of the IC50 calculation, 96% ethanol extract has the highest antioxidant activity at a concentration of 14.35 ppm (µg/mL) with a very high category. The content of secondary metabolites and very high antioxidant activity from the results obtained, explain that cashew nuts have the potential to increase body immunity and can be used in various antioxidant food products so that cashew leaves are more economically valuable for the community.

Takokak fruit contains bioactive compounds that can be used as antidiabetics. This study aims to identify bioactive compounds of ethanol extract of takokak fruit and antidiabetic test of n-Hexane fraction. The method used to obtain the extract is maceration using ethanol . Antidiabetic testing is carried out through in vivo testing with the Oral Glucose Tolerance Test (OGTT) method on white rats. Secondary metabolites are compounds contained in taakokak fruit that are identified as flavonoids, saponins, tannins, steroids and terpenoids . The results of the antidiabetic test showed that the n-Hexane fraction with glibencamide as a comparator has the ability as an antidiabetic in terms of the amount of difference in blood glucose levels in the test animal group from T 30 to T 120 . The average decrease in blood glucose levels in the negative control group (Na-CMC) was 108.67 mg/dL, the positive control group ( glibencamide ) 174.67 mg/dL, the 5% concentration fraction group 35.33 mg/dL, the 10% concentration fraction group 30.67 mg/dL, the 20% concentration fraction group 47.67 mg/dL.

Purple corn is one of the corn varieties that is being developed by Indonesia as a form of food diversification. The purple color of corn is produced by the high presence of anthocyanins so it can provide health benefits such as antioxidant, anti-inflammatory, mutagenic, anti-cancer and anti-angiogenesis. To ensure that the bioactive components are not damaged, extraction is carried out in stages by applying the maceration method using a polar solvent with the correct ethanol content of 96%. Apart from using the appropriate solvent, the length of maceration time will determine the results of the extract obtained. This research aims to find out how long maceration affects the content of bioactive compounds found in purple corn. The research used a completely randomized design with one treatment, namely maceration times of 12, 24 and 48 hours. The tests carried out included color intensity, total phenol and anthocyanin content and antioxidant activity. The research findings explain that the length of maceration has a significant effect on color intensity, total phenols and antioxidant activity of purple corn extract. 2 hour maceration was the best treatment in extracting bioactive compounds, namely total phenols of 206.29 g/100 g, total anthocyanins of 0.19 g/100 g and antioxidant activity of 58.80%.

Pear skin contains flavonoids which have potential as antioxidants. Considering the existence of humans as social creatures, interacting with each other requires self-confidence to be well accepted. For this reason, humans need self-care to appear charming, attractive and full of self-confidence. Masks are a type of cosmetic treatment that is well known and widely used. The mask works deeply to remove dead horn cells on the skin. This research aimed to determine the potential antioxidant activity of the ethanol extract of pear peel (Pyrus bretschneideri) which was formulated as a sheet mask preparation. Extraction was carried out by maceration using 96% ethanol to obtain pear peel extract. The antioxidant activity test method was carried out using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method. The results obtained from the concentration of the sheet mask preparation of pear peel extract (Phyrus bretschneideri) were homogeneous and stable. The pH results obtained from F0 (5,1), F1 (5,4), F2 (5,5), F3 (5,6), are still within the safe range of facial skin pH. The results of the antioxidant activity test showed that the IC50 value for F1 was 84.58 ppm with intensity (Strong), F2 69.96 ppm with intensity (Strong), and F3 63.79 ppm with intensity (Strong).