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Elisabet Bre Boli; Febriana Astuti; Charisa Sanggenafa; Cinta Resti Manda; Yerlin Wakerwa +10 more

Sevaka : Hasil Kegiatan Layanan Masyarakat 2026 STIKES Columbia Asia Medan

Breastfeeding should ideally begin immediately after birth and be provided exclusively for the first six months of life. However, in 2022, the coverage of exclusive breastfeeding among infants aged 0–6 months in Jayapura was only 21%, which remains far below the national target of 45%. Various factors contribute to this low coverage, including limited knowledge, lack of experience, insufficient support systems, and concerns among adolescent mothers that breastfeeding may interfere with their education or employment. This study aimed to explore the breastfeeding experiences of adolescent mothers in Wahno Village, Abepura Subdistrict. A qualitative research design was used, with structured interviews conducted using a questionnaire. The results indicated that the initiation of exclusive breastfeeding among adolescent mothers was generally positive. Participants reported mixed emotional experiences, including feelings of happiness, pride, stress, and discomfort. Family support, particularly from husbands, played an important role in sustaining breastfeeding practices. Most respondents also demonstrated adequate knowledge regarding how to manage breastfeeding-related challenges. Overall, adolescent mothers in Wahno Village showed a reasonable level of understanding and commitment toward exclusive breastfeeding. Continued education and support are recommended to further strengthen breastfeeding practices among adolescent mothers and their families.

Dian Mawarni

VitaMedica : Jurnal Rumpun Kesehatan Umum 2026 STIKES Columbia Asia Medan

The Society 5.0 era demands increased student health literacy as an essential competency. However, previous research has tended to focus only on the health education aspect and has not comprehensively examined the integration of technology-based health education management. Therefore, this study aims to analyze the effectiveness of technology-based health education management innovations and identify key factors influencing their successful implementation. This study used a mixed methods approach with a sequential explanatory design. Quantitative data were collected through a survey of 200 students, while qualitative data were obtained through in-depth interviews with 10 teachers and the principal. Quantitative data were analyzed using descriptive and inferential statistics, while qualitative data were analyzed using a thematic approach to strengthen the interpretation of the results. The results indicate that technology-based health education management innovations have a significant impact on improving student health literacy. Furthermore, it was found that successful implementation is influenced by five main factors: school institutional support, availability of technological infrastructure, teacher training, student motivation, and parental involvement. The novelty of this research lies in the integration of managerial and technological approaches in health education, tested through a mixed methods approach. These findings confirm that successful health literacy improvement is determined not only by the use of technology, but also by organizational readiness and multi-stakeholder collaboration. The implications of this research provide theoretical contributions to the development of technology-based health education management models and practical recommendations for educational institutions facing the challenges of Society 5.0.

Rahman, M Arief; Ahmad Ari Gunawan Sepriansyah; Sony Oktapriandi; Ambar Sehatiningsih; Jason Gunawan +3 more

Sevaka : Hasil Kegiatan Layanan Masyarakat 2026 STIKES Columbia Asia Medan

The socialization of Sultan Muda Sumsel Super Apps for OPD administrators was conducted to improve the capacity of administrators in managing website-based application content, data, and service information. The activity responded to the need for coordinated digital governance in supporting the 100,000 Sultan Muda program and the development of young entrepreneurship services in South Sumatra. The method consisted of need identification, material preparation, socialization, direct practice, and evaluation through discussion and technical assistance. The results show that the activity strengthened administrators’ understanding of application objectives, content management procedures, data accuracy, and follow-up mechanisms for digital publication. Documentation of the activity also reflects active mentoring, practical simulation, and coordination among stakeholders. The implication of this activity is the importance of continuous capacity building for OPD administrators to ensure that public digital service platforms are informative, updated, and aligned with institutional objectives.

Rahman, M Arief; Paisal; Wahyu Triaji Rahadianto; Melisa Kurnia Asfitri; Indah Pratiwi +1 more

Sevaka : Hasil Kegiatan Layanan Masyarakat 2026 STIKES Columbia Asia Medan

This community service activity aimed to strengthen the readiness of outstanding students of State Polytechnic of Sriwijaya in the Pilmapres 2026 selection through structured mentoring on achievement portfolio, innovative product, and English presentation. The program was designed as a participatory capacity-building activity involving needs identification, portfolio clinic, innovative product mentoring, English communication practice, presentation simulation, and reflective evaluation. The activity was implemented through technical meetings, document review, discussion, presentation practice, and feedback sessions involving students, supervisors, and institutional stakeholders. The results indicate that the mentoring process helped students organize evidence of achievement, sharpen the problem-solution logic of their innovative products, improve presentation confidence, and understand the assessment orientation of Pilmapres. Documentation of the activity shows the stages of preparation, selection, product presentation, and award ceremony. The program implies that continuous institutional mentoring is essential to build an ecosystem of student achievement, innovation, and English communication competence in vocational higher education.Keywords: Pilmapres; outstanding student; portfolio; innovative product; English presentation

Rahman, M Arief

Sevaka : Hasil Kegiatan Layanan Masyarakat 2026 STIKES Columbia Asia Medan

The iftar gathering within the Information Management academic community of Politeknik Negeri Sriwijaya was organized as a community service-based internal engagement activity to strengthen fellowship, interpersonal communication, and social concern. The program aimed to create a reflective and collaborative space for lecturers, staff, and students to build togetherness during Ramadan while reinforcing institutional values. The method consisted of coordination, preparation, implementation, documentation, and evaluation. The activity included opening remarks, informal dialogue, iftar, and social interaction among participants. The results indicate that the program contributed to improving collegial relationships, strengthening a sense of belonging, and encouraging social awareness among the academic community. The activity also provided a model of simple but meaningful institutional engagement that can be replicated in future academic and social programs. The implication of this activity is the need to maintain regular community-based programs as part of character development and organizational culture in higher education.

Syafrina Ulfah; Nurcholisah Fitra

VitaMedica : Jurnal Rumpun Kesehatan Umum 2026 STIKES Columbia Asia Medan

The Free Health Check-up (CKG) is a government program designed to strengthen promotive and preventive health efforts through the early detection of risk factors, pre-disease conditions, and diseases across the community according to the life-cycle approach. As a relatively new initiative, the implementation of the CKG program in primary healthcare settings still requires further evaluation to assess implementation readiness, digital support systems, and community participation. This literature review aimed to examine the implementation of the Free Health Check-up Program in primary healthcare services. Relevant literature was retrieved from the Google Scholar database using the keyword "implementation of the free health check-up program" and was limited to open-access, full-text articles published between 2025 and 2026. The search identified eight eligible articles, which were extracted and analyzed in this review. The synthesis revealed three major themes in the implementation of the CKG program: (1) primary healthcare service readiness, (2) digitalization and reporting systems, and (3) community participation and communication strategies. Primary healthcare readiness includes the availability of human resources, infrastructure, medical equipment, consumable medical supplies, funding, service workflows, and cross-sectoral coordination. Digitalization through the SATUSEHATplatform and integrated reporting systems has the potential to improve service efficiency; however, challenges remain regarding digital literacy, internet connectivity, and the technical capacity of healthcare personnel. Community participation is influenced by knowledge, attitudes, the roles of community health volunteers, teachers, healthcare workers, and communication strategies tailored to the characteristics of the target population. The successful implementation of the CKG program requires strengthening primary healthcare systems, optimizing digital health platforms, and adopting community-based communication strategies to ensure the program is more effective, inclusive, and sustainable.

Annisa Dwi Sandy; Febby Maharani; Sarah Adelia Hasibuan; Abdurrozzaq Hasibuan

VitaMedica : Jurnal Rumpun Kesehatan Umum 2026 STIKES Columbia Asia Medan

Industrial hygiene is a branch of public health research that aims to prevent illness and promote Industrial hygiene is an aspect of public health that prioritizes health protection measures for workers through the identification, evaluation, and control of various elements of the work environment that can cause health problems. The goal of implementing industrial hygiene is to prevent work-related illnesses and create a safe, healthy work environment that supports workers’ well-being. Industrial Safety and Health (IS&H) encompasses these various components, including risk identification, risk assessment, risk mitigation, and worker health monitoring, waste management, and emergency response planning. Workplace risks can include physical, chemical, biological, psychological, and ergonomic hazards. Hazard analysis is a crucial step in industrial hygiene as it helps identify and document potential health risks in the workplace. The hazard analysis process includes hazard identification, risk assessment, hazard prioritization, and risk control. The implementation of Occupational Safety and Health aims to ensure the protection of employees’ safety and health from potential hazards arising from work, while ensuring a safe and sustainable work environment. The implementation of OSH and environmental health also provides broad strategic benefits such as worker protection, cost efficiency, increased productivity, regulatory compliance, and enhanced corporate reputation.  

Rahman, M Arief; Ferizka Tiara Devani; Lailatur Rahmi; Ade Sukma Wati

Sevaka : Hasil Kegiatan Layanan Masyarakat 2026 STIKES Columbia Asia Medan

This community service article discusses the Student Creativity Program (PKM) Workshop for Informatics Management students of Politeknik Negeri Sriwijaya in 2026. The activity aimed to increase students’ capacity to design PKM ideas that are creative, logical, collaborative, and relevant to the problems of society and technology development. The method used a participatory workshop consisting of needs identification, material preparation, online implementation, discussion, idea clinic, and follow-up reflection. The workshop introduced the substance of PKM, proposal logic, theme selection, team collaboration, and the PKM-GFT scheme as a written futuristic idea that emphasizes visionary, realistic, solution-oriented, long-term, and broad-impact concepts. The results showed that students obtained a clearer understanding of PKM schemes, the characteristics of feasible ideas, and the importance of transforming simple observations into structured proposals. The activity also encouraged students to prepare ideas based on problems, novelty, benefits, and implementation logic. This workshop implies that continuous mentoring is needed to strengthen students’ proposal-writing skills, creativity, and competitiveness in PKM submission

Rahman, M Arief; Marti Utari; Ferizka Tiara Devani; Fitrianto Puja Kesuma; Nurul Ilma Hasana Kunio

Sevaka : Hasil Kegiatan Layanan Masyarakat 2026 STIKES Columbia Asia Medan

This community service activity aimed to improve communication competence and customer experience among service actors in a higher education environment. The training was implemented at Gedung Aula KPA Politeknik Negeri Sriwijaya on 19 February 2026 under the theme of excellent service transformation based on integrity to support the institutional commitment toward an Integrity Zone and Corruption-Free Area. The method consisted of preparation, needs identification, interactive presentation, case-based discussion, service communication simulation, and reflective evaluation. The material emphasized service attitude, active listening, empathy, complaint response, and customer experience touchpoints in academic and administrative services. The activity showed that participants were able to identify the importance of consistent communication behavior, emotional control, and service recovery in creating positive experiences for students, lecturers, partners, and public stakeholders. The implication of this activity is the need for continuous service culture reinforcement through standard operating procedures, role-play-based training, and periodic evaluation of user satisfaction in higher education services.

Nurcholisah Fitra

VitaMedica : Jurnal Rumpun Kesehatan Umum 2026 STIKES Columbia Asia Medan

Patient satisfaction is a key indicator of health service quality in the National Health Insurance era. Outpatient services for BPJS members still face problems regarding waiting time, communication, and facilities. This study aimed to analyze factors affecting BPJS patient satisfaction with outpatient service quality at Malahayati Islamic Hospital Medan in 2026. A cross-sectional analytic design was applied to 150 respondents selected through consecutive sampling. Data were collected using a validated questionnaire (r>0.361; α=0.876) and analyzed by Chi-square and multiple logistic regression. Results showed that waiting time (p<0.001), staff competence (p<0.001), staff attitude (p<0.001), drug availability (p=0.032), facility comfort (p<0.001), administrative ease (p=0.003), and information clarity (p=0.008) were significantly associated with satisfaction. Multivariate analysis revealed waiting time as the most dominant factor (OR=5.42; 95% CI: 2.31–12.71), followed by staff competence (OR=4.18), facility comfort (OR=2.87), and staff attitude (OR=2.42), with Nagelkerke R²=0.512. Hospital management should optimize service flow and adopt digital queuing systems to enhance patient satisfaction.

Nurcholisah Fitra; Syafrina Ulfah

VitaMedica : Jurnal Rumpun Kesehatan Umum 2026 STIKES Columbia Asia Medan

The development of Artificial Intelligence (AI) has driven significant transformation in hospital management, particularly in operational efficiency, service quality, and patient safety. This study aims to analyze the implementation of AI in hospital management based on recent scientific evidence from 2020 to 2026. The method used was a systematic review guided by the PRISMA 2020 framework. Literature was retrieved from PubMed, ScienceDirect, SpringerLink, Google Scholar, and ProQuest. From 360 identified articles, a stepwise selection process was conducted, resulting in 15 articles that met the inclusion criteria. The findings indicate that AI contributes to improved operational efficiency through patient flow optimization, operating room management, workforce scheduling, and electronic medical record management. AI also enhances service quality through predictive data analytics and supports patient safety through risk detection and early warning systems. In conclusion, AI has strong strategic potential to support modern hospital management. However, its implementation still faces several challenges, including human resource readiness, data security, algorithmic bias, system interoperability, and investment requirements. Therefore, AI implementation should be carried out in a planned, ethical manner and evaluated from a health economics perspective.

Nunung Setiyawati; Edy Susena

Jurnal Praba : Jurnal Rumpun Kesehatan Umum 2026 STIKES Columbia Asia Medan

The implementation of Electronic Medical Records (EMR) has become an essential part of digital transformation in the healthcare sector, aiming to improve the quality, effectiveness, efficiency, and continuity of healthcare services. In Indonesia, the implementation of EMR is supported by the Regulation of the Minister of Health Number 24 of 2022, which mandates healthcare facilities to adopt electronic medical records. However, the implementation process is often challenged by various technical and non-technical factors that may affect its success. This study aims to analyze the success factors and barriers to EMR implementation in hospitals through a literature review approach. The research method used was a literature review by examining and analyzing 11 scientific articles obtained from Google Scholar and other relevant scientific sources. The findings indicate that the success of EMR implementation is influenced by three main aspects, namely human, organizational, and technological factors. Supporting factors include management commitment, user readiness, continuous training, availability of information technology infrastructure, system quality, and organizational support. Meanwhile, common barriers include limited digital literacy among healthcare workers, insufficient information technology personnel, unstable internet connectivity, system errors, inadequate facilities and infrastructure, budget limitations, and the absence of specific standard operating procedures. Furthermore, EMR implementation provides significant benefits, such as improving service efficiency, facilitating access to patient information, enhancing documentation quality, supporting communication among healthcare professionals, and increasing patient safety. Therefore, successful EMR implementation requires synergy between human resources, organizational readiness, and technological infrastructure to achieve optimal healthcare service delivery.

Isnenia Isnenia; Meutia Mukarromah

Jurnal Praba : Jurnal Rumpun Kesehatan Umum 2026 STIKES Columbia Asia Medan

Proper drug disposal is often perceived as a trivial matter by the public when dealing with medications that are no longer needed. However, improper disposal can effect on the economy, public health, and the environment. Therefore, it is essential to educate the public to improve their knowledge regarding appropriate drug disposal methods. Previous studies have shown that educational media can effectively increase public awareness and understanding. The purpose of this study was to evaluate the effectiveness of educational leaflets in enhancing public knowledge about proper drug disposal. This experimental study involved an intervention using educational leaflets. A total of 100 respondents participated in the study. The intervention was conducted over three consecutive days. Pretest and posttest data were collected to assess changes in knowledge. Univariate analysis was used to describe respondent characteristics, while bivariate analysis (Wilcoxon test) was employed to compare pretest and posttest results. Pretest data indicated that the majority of respondents had only sufficient knowledge about drug disposal. Following the intervention, the number of respondents with sufficient knowledge decreased, while those categorized as having good knowledge increased. Statistically, a p-value of 0.000 demonstrated that the use of leaflets significantly improved knowledge level. Based on the results of this study, educational interventions, such as the distribution of leaflets, can effectively enhance public knowledge about proper drug disposal.

Luthfi Azhari; Wildan Maulana Assani Mualim; Muhammad Daffarezel Ramadhan; Pujo Santoso

JURNAL WILAYAH, KOTA DAN LINGKUNGAN BERKELANJUTAN 2026 Fakultas Teknik Universitas Cenderawasih

This study aims to synthesize empirical and theoretical literature on the Planning–Organizing–Actuating–Controlling (POAC) framework in public sector management, identify asymmetries among its functions, and propose a reconfiguration of POAC that is relevant to digital and collaborative governance. The study employs an integrative literature review by examining classical management literature, peer-reviewed journals, government regulations, and official governance indicators. Data were analyzed thematically based on the four POAC functions and synthesized across themes, using Indonesia during the 2021–2025 period as the empirical context. The findings reveal that planning and organizing functions have developed relatively well, while actuating and especially controlling remain persistent weaknesses. This condition is reflected in improvements in several formal governance indicators, including the Electronic-Based Government System (SPBE) Index, Indonesia’s ranking in the E-Government Development Index (EGDI), Unqualified Audit Opinions (WTP), and public service compliance ratings. However, during the same period, the Corruption Perceptions Index (CPI) declined. These findings indicate a gap between administrative achievements and substantive outcomes, consistent with the concept of means–ends decoupling in neo-institutional theory. The study contributes by bridging classical management theory with contemporary governance paradigms and proposing a Data-driven, Networked, Adaptive, and Participatory (DNAP) model of POAC. Practically, the results highlight the need to strengthen controlling functions and adaptive leadership to foster more effective, transparent, and outcome-oriented public governance.

Haryanto; Febrianti Sahar, Salsabila; Norita; Rizky Aulia Fitri, Nur; Ayu Pratiwi, Nabila +4 more

Jurnal Mahasiswa Ilmu Kesehatan 2026 STIKes Ibnu Sina Ajibarang

Batang prasman (Eupatorium triplinerve. Vahl) merupakan salah satu tanaman yang mengandung berbagai senyawa metabolit sekunder yang berpotensi memiliki aktivitas biologis, sehingga perlu dilakukan pengujian toksisitas sebagai langkah awal untuk mengetahui tingkat keamanannya. Penelitian ini bertujuan untuk menentukan nilai LC₅₀ dan kategori toksisitas ekstrak etanol batang prasman terhadap larva udang (Artemia salina Leach) menggunakan metode analisis probit. Penelitian dilakukan secara eksperimental laboratorik dengan metode Brine Shrimp Lethality Test (BSLT). Ekstrak etanol batang prasman diuji pada konsentrasi 100, 500, 1.000, 5.000, dan 10.000 ppm. Parameter yang diamati adalah jumlah kematian larva setelah 24 jam perlakuan. Data mortalitas dihitung dalam bentuk persentase kematian, kemudian dianalisis menggunakan metode probit untuk menentukan nilai LC₅₀. Hasil penelitian menunjukkan bahwa persentase kematian larva meningkat seiring dengan peningkatan konsentrasi ekstrak, yaitu sebesar 25%, 35%, 50%, 75%, dan 85%. Hasil analisis probit menunjukkan nilai LC₅₀ ekstrak etanol batang prasman sebesar 846 mg/L. Berdasarkan klasifikasi toksisitas, nilai tersebut termasuk dalam kategori moderat atau racun sedang. Dengan demikian, ekstrak etanol batang prasman (Euphatorium triplinerve Vahl) memiliki aktivitas toksik terhadap larva Artemia salina Leach dan termasuk dalam kategori toksisitas sedang

Heru Fahrudin Faiz; Rika Ampuh Hadiguna

JURNAL WILAYAH, KOTA DAN LINGKUNGAN BERKELANJUTAN 2026 Fakultas Teknik Universitas Cenderawasih

Road improvement projects require consistency between technical planning documents and field implementation to ensure that service quality, structural performance, construction time, and user safety are achieved. This article evaluates the conformity between planning and realization in the Pangkalan-Batas Jambi road improvement project in Rawas Ulu District, North Musi Rawas Regency. The study used a descriptive-quantitative evaluative approach based on field monitoring, project technical documents, comparative quantity analysis between contract and Contract Change Order (CCO), and identification of implementation constraints. The results showed that several work items changed during construction because initial planning data did not fully capture actual field conditions. Quantities increased for drainage excavation (+18.87%), roadbed preparation (+8.99%), aggregate class A base course (+12.51%), and AC-Base (+11.98%). Conversely, reinforced pipe culverts decreased (-58.33%), unsealed aggregate base/sirtu decreased (-43.50%), and tack/prime coat volume slightly decreased (-0.47%). The time schedule showed a minor delay of -0.13% in the first week but recovered in the second week, indicating effective field coordination. Main constraints included traffic interference, limited worker visibility, and equipment visibility. The study recommends more detailed site surveys during planning, stronger traffic management, stricter occupational safety implementation, systematic progress control, and routine post-construction maintenance to maintain road service life.

Dwi Eri Yanti; Vera Surtia Bachtiar; Alfirmansyah Alfirmansyah; Ummi Jayanti

JURNAL WILAYAH, KOTA DAN LINGKUNGAN BERKELANJUTAN 2026 Fakultas Teknik Universitas Cenderawasih

The governance of sand and gravel mining requires an integrated assessment of technical planning, occupational safety, environmental control, reclamation, and regulatory compliance. This study evaluated the governance of CV. Ria Bersaudara, a sand and gravel mining company located in Pasar Surulangun, Rawas Ulu District, Musi Rawas Utara Regency, using the Good Mining Practice framework. The study used a descriptive evaluative approach based on field observation, document review, indicative resource estimation, equipment productivity analysis, occupational safety and environmental risk assessment, and compliance mapping. The results show that the company has a basic legal foundation through an exploration mining permit covering 12.5 ha; however, several governance components require improvement. The indicative prospective area was approximately 2.50 ha, with an effective follow-up area of 1.80 ha and an estimated indicative resource of 21,600 m³ or 35,640 tons. Productivity analysis indicated that excavator capacity reached about 61 m³/hour, while one dump truck only transported about 10.3 m³/hour, creating a haulage bottleneck if the truck fleet is insufficient. Safety implementation was also not optimal, with personal protective equipment compliance estimated at only 55%. The study recommends validating permit documents, strengthening technical exploration data, improving drainage and sediment control, enforcing safety procedures, implementing progressive reclamation, and establishing daily operational records.

Haryanto; Amalia, Fitrah; Ramadhan, Ahmad; Kartika Ayu Ridwan, Maretta; Hatima, Husnul +3 more

Jurnal Mahasiswa Ilmu Kesehatan 2026 STIKes Ibnu Sina Ajibarang

Penelitian ini bertujuan untuk mengkarakterisasi tingkat toksisitas ekstrak etanol 90% daun trembesi (Samanea saman) terhadap larva Artemia salina melalui penentuan nilai LC₅₀ menggunakan metode Reed-Muench dan analisis probit. Penelitian ini merupakan penelitian eksperimental dengan rancangan post-test only control group design. Larva Artemia salina digunakan sebagai organisme uji dan dipaparkan pada berbagai konsentrasi ekstrak, yaitu 10 ppm, 100 ppm, 500 ppm, 1000 ppm, 5000 ppm, dan 10.000 ppm. Parameter yang diamati adalah jumlah kematian larva setelah 24 jam perlakuan. Hasil penelitian menunjukkan bahwa persentase kematian larva meningkat seiring dengan kenaikan konsentrasi ekstrak, yang mengindikasikan adanya hubungan dosis-respons. Perhitungan nilai LC₅₀ dengan metode 823 ppm. Berdasarkan klasifikasi toksisitas Brine Shrimp Lethality Test (BSLT), nilai LC₅₀ tersebut termasuk dalam kategori toksik moderat / sedang. Hal ini menunjukkan bahwa ekstrak etanol 90% daun trembesi memiliki aktivitas toksisitas sedang terhadap larva Artemia salina. Dengan demikian, ekstrak ini berpotensi sebagai sumber senyawa bioaktif yang dapat dikembangkan lebih lanjut namun diperlukan penelitian lanjutan untuk mengidentifikasi senyawa aktif serta mengevaluasi keamanan pada sistem biologis yang lebih kompleks.

Haryanto; Arkam Runa Bombing, Mychellianien; Nurica; Ramadhani Putri Syahyuti, Nia; Nurul Aliyah , Afni +2 more

Jurnal Mahasiswa Ilmu Kesehatan 2026 STIKes Ibnu Sina Ajibarang

Penelitian ini bertujuan untuk menentukan nilai LC₅₀ ekstrak etanol 70% daun trembesi ( Samanea saman ) serta mengklasifikasikan tingkat toksisitasnya menggunakan metode Reed-Muench dan analisis probit. Uji toksisitas dilakukan terhadap larva Artemia salina sebagai organisme uji dengan variasi konsentrasi ekstrak yaitu 10, 100, 500, 1000, 5000, dan 10.000 ppm. Parameter yang diamati adalah jumlah kematian larva setelah 24 jam perlakuan. Hasil penelitian menunjukkan bahwa peningkatan konsentrasi ekstrak berbanding lurus dengan peningkatan persentase kematian larva, yang mengindikasikan adanya hubungan dosis-respons yang jelas. Perhitungan menggunakan metode Reed-Muench dan analisis probit menghasilkan nilai LC50 sebesar 100,46 ppm. Berdasarkan klasifikasi toksisitas metode Brine Shrimp Lethality Test (BSLT), nilai tersebut termasuk dalam kategori toksik sedang (moderatelytoxic). Hal ini menunjukkan bahwa ekstrak etanol 70% daun trembesi memiliki aktivitas biologi yang signifikan terhadap organisme uji. Dengan demikian, ekstrak ini berpotensi untuk dikembangkan sebagai sumber senyawa bioaktif, namun tetap memerlukan kajian lebih lanjut terkait keamanan dan aplikasinya.