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The consumption of sugar-sweetened beverages (SSBs) in Indonesia continues to increase and is associated with the risk of obesity, diabetes, and cardiovascular diseases due to oxidative stress. Kombucha has potential as a functional antioxidant beverage; however, its use is still dominated by Camellia sinensis, limiting the utilization of local ingredients. Bilimbi leaves (Averrhoa bilimbi Linn.) have high antioxidant activity and potential as an alternative substrate. This study aimed to analyze the effect of fermentation time variations (4, 7, and 14 days) and sugar concentrations (10% and 15%) on the acceptability and antioxidant activity of bilimbi leaf kombucha, as well as to determine the best formulation. The study used a true experimental design with a Completely Randomized Design (CRD) involving six formulations (F1–F6) and one control (F0). Antioxidant activity was analyzed using the DPPH method. The results showed that fermentation time and sugar concentration significantly affected antioxidant activity.). The highest antioxidant activity was found in F2 (IC50 98.67 ppm, strong category). F2 was determined as the best formulation due to its  antioxidant activity. F2 has the potential to be developed as an alternative functional beverage to reduce SSB consumption. Further research is recommended to conduct stability testing and in vivo studies.

The consumption of sugar-sweetened beverages (SSBs) in Indonesia continues to increase and is associated with the risk of obesity, diabetes, and cardiovascular diseases due to oxidative stress. Kombucha has potential as a functional antioxidant beverage; however, its use is still dominated by Camellia sinensis, limiting the utilization of local ingredients. Bilimbi leaves (Averrhoa bilimbi Linn.) have high antioxidant activity and potential as an alternative substrate. This study aimed to analyze the effect of fermentation time variations (4, 7, and 14 days) and sugar concentrations (10% and 15%) on the acceptability and antioxidant activity of bilimbi leaf kombucha, as well as to determine the best formulation. The study used a true experimental design with a Completely Randomized Design (CRD) involving six formulations (F1–F6) and one control (F0). Antioxidant activity was analyzed using the DPPH method. The results showed that fermentation time and sugar concentration significantly affected antioxidant activity.). The highest antioxidant activity was found in F2 (IC50 98.67 ppm, strong category). F2 was determined as the best formulation due to its  antioxidant activity. F2 has the potential to be developed as an alternative functional beverage to reduce SSB consumption. Further research is recommended to conduct stability testing and in vivo studies.

This study analyzes the strategic role of quality-based marketing models in enhancing the competitiveness of kombucha products, with a specific focus on Rumah SCOBY DBA, produced by the Yayasan Dharma Bintang Akademia. By integrating Total Quality Management (TQM), Quality Assurance (QA), and Quality Control (QC), the research explores how quality-driven frameworks contribute to marketing effectiveness, consumer trust, brand positioning, and sustainable performance in the functional beverage industry. Employing a Systematic Literature Review (SLR) combined with conceptual analysis, the study systematically examined publications from 2015 to 2025 across leading academic databases, focusing on themes of quality management, functional beverage marketing, kombucha production, consumer behavior, and digital strategies. The findings reveal that product quality—characterized by fermentation stability, microbiological safety, and nutritional consistency—serves as the primary driver of consumer purchase intention. Process quality, through standardized SOPs, hygiene protocols, and traceability systems, reinforces credibility, while service quality, including transparent labeling, health education, and digital engagement, strengthens brand trust. Integrating TQM principles into marketing fosters consumer loyalty, differentiates brands in competitive markets, and supports long-term sustainability. This study provides practical guidance for producers, community-based enterprises, and policymakers to adopt quality-driven marketing models, offering a novel conceptual framework tailored to kombucha products and mapping future research directions in functional beverage innovation.

This article examines the effectiveness of fermented kombucha drinks in enhancing the immunity of menopausal women through an evidence-based nutrition approach. Additionally, the study highlights how community-based kombucha production and consumption programs can be part of non-military health diplomacy, supporting community resilience, especially for vulnerable groups like menopausal women who experience immune function decline due to hormonal changes. Using the Systematic Literature Review (SLR) method, the study analyzes 30 relevant articles published between 2018–2025 from reputable sources such as Scopus, PubMed, and others. The findings show that (1) kombucha improves immunity through cytokine modulation and increased antioxidant activity, (2) kombucha normalizes gut microbiota, contributing to the immune health of menopausal women, (3) fermentation variations and microbial composition affect kombucha's effectiveness, and (4) community-based kombucha production programs have potential in enhancing health literacy and local economic empowerment. This study provides a basis for local governments, health centers, and communities to integrate kombucha as a preventive health intervention, while also strengthening community resilience through nutrition education and functional food entrepreneurship. The article also offers a new conceptual model, “Kombucha Intervention – Immunity – Health Diplomacy,” that connects biological evidence, community health, and non-military resilience integratively.

This study aims to analyze the effect of nutrient types on kombucha fermentation using Sosro Tea as a medium. The practicum was conducted with two variations, namely honey as a natural nutrient and Tropicana Slim as a low-calorie sweetener. Parameters observed included color changes, aroma, SCOBY layer formation, pH, and microbial contamination. The results showed that fermentation using honey produced the best combination, which was characterized by a brighter color change, a stable sour aroma, the formation of a thick and pure white SCOBY, and a stable pH of around 4. In contrast, fermentation using Tropicana Slim failed to achieve optimal results. This low-calorie sweetener cannot be metabolized by microbes, resulting in a thin SCOBY and the appearance of green-black mold. Based on these results, it can be concluded that the success of kombucha fermentation is greatly influenced by the availability of simple sugars, which are the main energy source for the SCOBY. In addition, the culture's resistance to microbial contamination also plays an important role in the fermentation process.

Kombucha is one of the processed products of fermented sugar and tea with a starter mixture ofkombucha culture called SCOBY (Symbiotic Cultures of Bacteria and Yeast). Kombuchafermentation is carried out using yeast and bacteria microorganisms. Pomegranate or often calledpomegranate is a fruit that has quite high antioxidants. The purpose of this study was to determinethe characteristics of pomegranate juice kombucha with variations in concentration andfermentation time which have high antioxidant activity. This study used a factorial randomizeddesign with two factors and two repetitions. The factors used were variations in the percentage ofpomegranate juice (30%, 40%, and 50%) and fermentation time (7 days, 9 days, and 11 days). Thebest chemical and organoleptic analysis based on the highest antioxidant activity was 50%pomegranate juice treatment and 9 days of fermentation time with 71,10% antioxidant activity,total phenol 9,04 GAE/L, reducing sugar 2,45%, total acid 1,35%, pH 3,1 , anthocyanin 53.52%,lightness (L*) 44.72 , redness (a*) 5.15, yellowness (b*) 2.57, fruity aroma 2.27, sour taste 2,85, andfor overall preference 3,49 for the treatment of adding 50% pomegranate juice and 9 days offermentation time. Pomegranate extract able to improve activity of antioxidant for kombucha. 

This study aimed to evaluate the effect of varying honey concentrations on the antioxidant activity, chemical properties, and organoleptic quality of a probiotic kombucha beverage prepared from Clitoria ternatea L. The research was motivated by increasing exposure to free radicals due to environmental pollution and the demand for alternative natural antioxidants. Kombucha was produced with honey concentrations of 10%, 20%, and 30% (w/v), and analyses were performed using the DPPH assay for antioxidant activity, measurements of pH, total acidity, and total phenolic content for chemical properties, as well as sensory evaluations by trained panelists. The results showed that kombucha with 10% honey exhibited the lowest IC₅₀ value, indicating the highest antioxidant activity, while higher concentrations increased the total phenolic content without significantly enhancing the effective antioxidant potential. These findings suggest that excessive honey addition does not necessarily improve the functional benefits of the product, indicating the need for further formulation optimization to develop a high-quality, marketable probiotic beverage.

Kombucha tea is a drink produced through a fermentation process of a mixture of tea and sugar using kombucha culture or also called SCOOBY ( Symbiotic Culture of Bacteria and Yeast) . The basic ingredients for making kombucha tea are granulated sugar solution and tea bags, in this study it was varied using fruit skin. Vitamin C is known as an important antioxidant found in many fruits, including in rujak fruit skin which is often considered waste. The purpose of this study was to determine the levels of vitamin C in kombucha tea fermented from fruit skin waste. This study used qualitative and quantitative methods. The qualitative method uses the Benedict test and the quantitative method uses the calculation of the percentage of vitamin C content using the iodimetric titration method. The results of the qualitative analysis of the Benedict test were positive for vitamin C with a change in the color of the sample solution to green-yellowish and the analysis of the percentage of vitamin C content obtained a result of 2.3124% or equal to 0.23124 mg / 10 mL of sample solution. The vitamin C content of kombucha tea can vary depending on the base ingredients used, fermentation time, and storage temperature.

Kombucha tea is a traditional beverages product manufactured from fermented tea and sugar solutions. Using Acetobacter xylinum sa starter. Kombucha tea has a refreshing sour taste, but the distinctives aroma make kombucha tea less attractives to consumers. Red roses (Rosa damascene) are a source of anthocyanin pigments that have not been widely used.  In addition, to increase the economic value of roses.  Based on research that has been done, red roses contain antioxidants in the form of purplish red anthocyanins.  The purpose of this study was to determine the effect of the type of sugar and the concentration of rose extract on the characteristics of rose kombucha produced.  This study used Completely Randomized Design (CRD). The factors used were the concentration of rose tea (1g/250ml, 2g/250ml, 3g/250ml) and variations in the type of sugar (palm, sand, fine).  The best chemical and organoleptic analysis based on the highest antioxidant activity was the addition of palm sugar and the concentration of the addition of rose tea 1g/250ml with a total phenolic of 10.17mg GAE/L, total dissolved solids (TPT) 11.10%, acidity (pH) 3.86, antioxidant activity 48.28%, vitamin C 11.41%, 13.63% for total sugar, sour taste score 3.06, rose flavor 2.15 and 1.10 for overall preference the addition of palm sugar and the concentration of the addition of rose tea 1g/250ml. Kombucha made from rose extract can be an alternative functional food.

Pada tanggal 7 Desember 2022 telah dilakukan kegiatan pengabdian kepada masyarakat dalam bentuk penyuluhan dan cara pembuatan Kombucha. Pengabdian ini bertujuan untuk memberikan edukasi kepada mayarakat Desa Moncongloe Bulu’ kecamatan Moncongloe Kabupaten Maros tentang manafaat Kombucha dalam peningkatan fungsi fisiologi tubuh.Metode yang dilakukan dalam pengabdian ini yaitu memberikan solusi dan pengetahuan khususnya di bidang kesehatan mengenai manfaat Kombucha. Peserta dalam program pengabdian ini sangat antusias dalam menerima dan melaksanakan tahapan yang dipaparkan. Peserta pada pengabdian ini adalah pegawai kantor desa, ibu PKK, KadeR Desa dan Mahasiswa KKN. Tahapan-tahapan yang dilaksanakan pada program pengabdian ini telah membantu meningkatkan pengetahuan masyarakat Desa Moncongloe Bulu’  khususnya di Kacematan Moncongloe mengenai proses pembuatan Kombucha dan mengetahui manfaat kesehatan yang di hasilkan dalam mengkomsumi dan menggunakan Kombucha.

Pharmaceutical biotechnology products in the form of hand washing soap with an active ingredient of telang flower kombucha fermented solution have the potential to inhibit the growth of Staphylococcus capitis, Bacillus cereus, and Pantoea dispersa bacteria. The aim of this study was to formulate and prepare hand washing soap with an active ingredient in a solution of seagrass kombucha fermentation to inhibit the growth of Staphylococcus capitis, Bacillus cereus, and Pantoea dispersa. This research is an experimental laboratory by making 4 hand washing soap preparations, which consist of a hand washing soap base without an active substance. Hand washing soap base added to a solution of fermented butterfly pea flower kombucha at a sugar concentration of 20%, 30%, and 40%. Disc diffusion is a method used to calculate the average diameter of the inhibition zone for the three tested bacterial growth. The research data obtained were analyzed through one way ANOVA and post hoc analysis. The results of the ANOVA test proved that the obtained p value <0.05, which means that there were significant differences in the three treatment groups in inhibiting the growth of the three test bacteria. The post hoc analysis results also proved that 40% concentration was the best concentration in inhibiting the growth of the three tested bacteria, namely 18.34 mm (S. capitis/strong), 16.39 mm (B. cereus/strong), and 13.33 mm (P. disperse/strong).

Telang flower has the potential to inhibit the growth of Candida albicans, Malasezia furfur, Pitosporum ovale, and Aspergilus fumigatus through the kombucha fermentation biotechnology method. The purpose of this study was to determine the antifungal activity of telang flower kombucha at various concentrations of white sugar. The varying concentrations of granulated sugar used in this study were 20%, 30%, and 40% (w/v), each repeated 3 times. The positive control used was kombucha made from green tea. The negative control used was sterile distilled water. The well diffusion method is one of the methods used to test antifungal activity. The fementation of telang flower kombucha has overall antifungal activity. White sugar concentration of 40% kombucha telang flower was the best concentration in inhibiting the growth of fungi as a whole when compared to the concentration of white sugar kombucha telang flower of 20%, 30% and positive and negative controls.