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Nurul Sugiarti Syam; Syamsuryanita; Nurul Ikawati

Jurnal Fisioterapi dan Ilmu Kesehatan Sisthana (JUFDIKES) 2021 Stikes Kesdam IV/Diponegoro Semarang, Indonesia

  Perkembangan merupakan perubahan yang terjadi pada diri individu dengan bertambahnya kemampuan dan fungsi tubuh dari yang sederhana ke yang lebih kompleks, sebagai hasil dari proses pematangan. Tujuan penelitian ini yaitu untuk mengetahui Pengaruh Pijat Bayi Terhadap Peningkatan Motorik Kasar Pada Bayi Usia 3-6 Bulan di BPM Suriyanti, S.ST Makassar. Desain Penelitian ini adalah eksperimen metode Pre Test-Post Test Control Group Design, populasi penelitian ini berjumlah 100 bayi di BPM Suriyanti, S.ST Makassar dan sampel berjumlah 30 orang. Hasil penelitian menunjukkan bahwa dari 15 orang (50%) yang mendapatkan pijat bayi, terdapat 14 bayi (93,3%) yang motorik kasanya meningkat dan 1 bayi (6,7 %) yang tidak meningkat. Sedangkan yang tidak di lakukan pijat bayi sebanyak 15 bayi (50%), terdapat 2 orang motorik kasarnya meningkat (13,3%) dan 13 bayi (86,7%) yang tidak meningkat. Dengan menggunakan uji Mann-Whitney dan wilcokxon, menunjukkan bahwa setelah diuji wilcoxon nilai ? Value pretest-posttest dari kelompok perlakuan adalah 0,000 (<0,05), sedangkan nilai ? Value pretes-posttest pada kelompok kontrol 0.039 (<0,05). Sedangkan setelah diuji Mann whitney didapatkan hasil ? = 0,000 (<0,05). Maka dapat disimpulkan bahwa ?<?, sehingga Ho ditolak dan Ha diterima. yang artinya ada pengaruh pijat bayi terhadap peningkatan motoric kasar pada bayi usia 3-6 bulan

Fasridon Fasridon; Yuli Angraini

KOMPAK : Jurnal Ilmiah Komputerisasi Akuntansi 2021 Universitas Sains dan Teknologi Komputer

This study aims to examine the effect of structure on ownership, profitability, and capital structure of firm value on property and real estate listed on the Indonesia Stock Exchange in 2016-2020. The sample selection technique used porpusive sampling and obtained as many as 30 Property and Real Estate Companies. The data analysis technique used panel data regression analysis using Eviews 10.0. Based on the results of partial hypothesis testing, it was found that the ownership structure had no significant effect on firm value. Profitability has a positive and significant effect on firm value. Capital structure has a positive and significant effect on firm value. Based on the hypothesis, simultaneously ownership structure, profitability and capital structure have a positive and significant effect on firm value in Property and Real Estate Companies listed on the Indonesia Stock Exchange in 2016-2020. The contribution of the independent variables of ownership structure, profitability and capital structure to the dependent variable of firm value is 0.80213 or 80%. Meanwhile, 20% is influenced by other variables outside the study. It is hoped that this research can help properties and real estate listed on the Indonesia Stock Exchange to increase firm value by considering factors that have a significant effect on profitability such as ownership structure, profitability, and capital structure.

Subagyo, Herry

KOMPAK : Jurnal Ilmiah Komputerisasi Akuntansi 2021 Universitas Sains dan Teknologi Komputer

This research aims to test the relation between ownership structures and capital structure firm value of basic industry & chemistry firm. This research places the capital structure as a mediating variable for the relationship between ownership structure and firm value. The sample used is a company listed on the Indonesia Stock Exchange for the period 2014-2019, using a purposive sampling technique got 115 samples that meet the criteria. Data processing using the IBM SPSS 23 series program as an analysis tool. The result is that management's ownership has a negative effect on capital structure, and a positive effect on firm value, while institutional ownership has a positive effect on capital structure, and a negative effect on firm value. This study also found that capital structure is a mediating variable of the relationship between management ownership and firm value. Keywords: ownership structure, capital structure, firm value  

Ira Zulfa; Hendri Syahputra; Ahyar Faisal

Jurnal Elektronika dan Komputer 2021 STEKOM PRESS

The purpose of this thesis is to produce tangible results in providing comfort or convenience in controlling electrical appliances, including turning on or off lights at home and controlling other electrical appliances. The method used in making the Design of Control System for Electrical Equipment Using Bluetooth Based on the Atmega 328 Microcontroller is a prototype. This research method consists of several stages, library research, analytical methods, design and implementation methods. The results achieved are reducing the number of fires due to electrical short circuits and increasing aspects of comfort and convenience for users as well as people with disabilities and the elderly, who find it difficult to stand or to reach lights and the implementation of infrastructure devices, where a series of these tools can work after bluetooth devices are available in the community. android smartphones. connected to the bluetooth module connected to the arduino microcontroller. Basically, using the principle of this tool, the user must be able to operate a smartphone based on the Android operating system. The conclusion is that this equipment has been tested and can be used as a controller of power tools with a lightweight android smartphone via a Bluetooth connection without having to press the switch button.

Fardelia Safira, Della; Tituk Diah Widajantie

EBISNIS : JURNAL ILMIAH EKONOMI DAN BISNIS 2021 LPPM Universitas Sains dan Teknologi Komputer

The Company has short-term and long-term. In the short term the company aims to maximize current profits, while in the long term it aims to increase the value of the company itself. This research aimed to examine and analyze the effect of profitability, company size, leverage, and CSR disclosure to the value of manufacturing companies listed on the IDX in 2015-2019. While, the sampling collection technique used purposive sampling with 10 samples which fulfilled the criteria. The data analysis technique used multiple regression linear with SPSS (Statistical Product and Service Solutions). In this research, the testing variable of profitability used Return On Asset (ROA), company size used total assets, leverage used Debt to Equity Ratio (DER), CSR used Corporate Social Disclousure Index (CSDI), and the value of the company used Tobins’Q. The research result concluded that: (1) profitability effect the value of the company, (2) company size did not effect the value of the company, (3) leverage did not effect the value of the company, (4) CSR disclosure effect the value of the company. Keywords: Profitability, Company Size, Leverage, CSR Disclosure, and the value of the company.

Tumanan, Natalia; Tumanan, Natalia; Dyah Ratnawati

EBISNIS : JURNAL ILMIAH EKONOMI DAN BISNIS 2021 LPPM Universitas Sains dan Teknologi Komputer

This research is meant to test the influence of liquidity, profitability, and leverage to the firm value with the corporate social responsibility as the moderating variable. Firm value describes the prosperity of the shareholders. Liquidity, profitability, leverage and corporate social responsibility are the factors which can be a consideration for investors in investing. Methods that used in this research is quantitative method using secondary data obtained through the company’s financial statements. The result of the research shows that: 1) Liquidity has an influence to the firm value. 2) Profitabilitry has an influence to the firm value. 3) Leverage has an influence to the firm value. 4) Corporate social responsibility is unable to moderate the influence of liquidity to the firm value. 5) Corporate social responsibility is unable to moderate the influence of profitability to the firm value. 6) Corporate social responsibility is unable to moderate the influence of leverage to the firm value.

Alawiyah, Rieza Wardah; Alawiyah, Rieza Wardah; Tituk Diah Widajantie

EBISNIS : JURNAL ILMIAH EKONOMI DAN BISNIS 2021 LPPM Universitas Sains dan Teknologi Komputer

This study aims to determine the effect of Obedience Pressure, Independence, and Audit Expertise on Audit Judgment at Public Accounting Firms in the East Surabaya Region. The population of this research is all auditors who work at the Public Accounting Firm in the East Surabaya Region which consists of 28 Public Accounting Firms that are registered in the Public Accounting Firm Information System at the Indonesian Institute of Public Accountants with the Ministry of Finance of the Republic of Indonesia Secretariat General of the Center for Financial Professional Development. These are 40 auditors who work at 9 Public Accounting Firms in the East Surabaya Region. This study uses the Structural Equation Model (SEM) model with the Partial Least Square (PLS) analysis model to test the previously proposed hypotheses. PLS analysis was tested using SmartPLS version 2.0 for windows software. Based on the results of the research that has been done, it can be concluded as follows: that Obedience Pressure on Audit Judgment is proven to have a significant negative effect. Independence from Audit Judgment is proven to have a significant positive effect. Audit expertise on Audit Judgment is proven to have a significant positive effect.

Cici Farhana Ambarwanty Mohtar; Epsi Marwati; Delita Septining Tyas

Jurnal Sains dan Kesehatan (JUSIKA) 2021 Universitas Muhamadiyah Manado

Tuberculosis is a lung infection caused by the bacteria Mycobacterium tuberculosis. Early detection is one of the ways to detect tuberculosis. The first detection of tuberculosis is with the Molecular Rapid Test, which is one of the technologies being developed using GeneXpert. Patients with positive Molecular Rapid Test results will be treated with rifampicin for 6 months, then further screening of Acid-Fast Bacilli microscopically will be carried out to determine whether the patient is still positive for pulmonary tuberculosis or not. The purpose of this study was to determine the relationship between acid-fast bacillus examination in tuberculosis patients with positive molecular rapid test results. This study will be conducted experimentally. The results of calculations using the Wilcoxon Test showed that there was a relationship between TCM examination results and BTA follow-up screening in TB patients after and before rifampicin treatment for 6 months.    

Ikhwani Ikhwani; Supriyatin Supriyatin; Misika Alam

Jurnal Sains dan Kesehatan (JUSIKA) 2021 Universitas Muhamadiyah Manado

Abstract. Gout often attacks people over the age of 30, this is because at that age the declining esterogenous levels can cause uric acid levels in the blood to increase. High uric acid levels can be overcome with a variety of treatments including nonpharmacological treatment in the form of giving pineapple juice. Prevention that can be done to keep uric acid levels normal is by limiting purine foods, choosing low-purine foods, avoiding taking drugs, and taking vitamin C supplements including consuming pineapple juice. The purpose of this study was to determine uric acid levels before and after giving pineapple juice and to determine the difference in results before and after giving pineapple juice on uric acid levels in menopausal women. The research method was pre experiment and the method of examining uric acid levels using the POCT method. The population in the study were 25 menopausal women aged 45-55 years in RW 3 Kaliwadas Village, Cirebon Regency. The sample in the study was 22 menopausal women, with the sampling technique using purposive sampling. Data from the examination of uric acid levels before and after the administration of pineapple juice obtained the results of 21 samples that experienced a decrease (95.45%) and 1 sample that experienced an increase (4.45%) with an average uric acid level before 5.6 mg/dL with the highest level of 8.4 mg/dL and the lowest level of 4.7 mg/dL and the average after 5.3 mg/dL with the highest level of 8.4 mg/dL and the lowest level of 3.7 mg/dL. The research data were analyzed using a normality test and paired sample T-test obtained Sig. (2-tailed) 0.000 <0.005. based on this study it can be concluded that there is an influence on uric acid levels before and after being given pineapple juice.

Iin Handayani; Harlina Harlina; Abdul Manaf; La Ode Maman

Jurnal Pengabdian Masyarakat Nusantara (Pengabmas Nusantara) 2021 Universitas Muhammadiyah Manado

Bipolar disorder is a mental health condition characterized by extreme mood swings between manic and depressive episodes. Coastal communities often face limited access to mental health information and services, leading to a lack of awareness and early detection of this disorder. This community service program aims to enhance awareness and understanding of bipolar disorder among adolescents in the coastal area of Baubau City through a community-based counseling approach. The methods used in this program include socialization, interactive discussions, case simulations, as well as pre-test and post-test evaluations to measure program effectiveness. The results showed that after attending the counseling session, participants' understanding of bipolar disorder increased by 85%, while the community's readiness to seek professional help increased from 30% to 75%. Additionally, positive social changes occurred, such as reduced stigma against mental disorders and the emergence of community leaders who actively promote mental health education. These findings indicate that a community-based educational approach can be an effective strategy in improving mental health literacy, particularly in areas with limited access to psychological services. Therefore, it is recommended that continuous counseling programs and further collaborations between the government, healthcare professionals, and local communities be implemented to ensure the sustainability of this program.

Hidayat, Tauhid

Tujuan dari penelitian ini adalah mengetahui implementasi Metode Komunikasi Verbal dan Non Verbal untuk meningkatkan Motivasi Berprestai dan Hasil Belajar TPQ Tentang Terjemah Surat An nas pada Siswa kelas 1E semester I SD Muhammadiyah 2 Denpasar. Subjek penelitian ini adalah siswa kelas 1 sebanyak 32 orang. Data yang dianalisis menggunakan deskriptif kualitatif untuk menunjukan rata–rata skor motivasi berprestasi, hasil belajar, dan kriteria ketuntasan yang digambarkan dalam bentuk grafik poligon. Hasil penelitian ini menunjukan bahwa, dari hasil observasi tentang motivasi belajar sebelum penelitian hanya 60% pada siklus I menjadi 91% sedangkan Prestasi belajar dari post test mulai nilai 61,3 naik menjadi 69,1 ( siklus-I ) kemudian naik lagi menjadi 80,9 pada siklus – II sedangkan ketercapaian ketuntasan belajar dari 40,6% menjadi 65,6% dan pada Siklus-II menjadi 90,6%, dapat disimpulkan bahwa Metode Komunikasi Verbal dan Non Verbal dapat diterapkan untuk meningkatkan motivasi berprestasi dan Hasil belajar, disamping itu untuk menciptakan suasana yang menyenangkan. sehingga secara bertahap prestasi Tahfidz juz 30 peserta didik dapat meningkat. ABSTRACTThe purpose of this research is to know the implementation methods of Verbal and Non Verbal Communication to increase Motivation Achievement and Learning Results TPQ About translations of the An nas in the grade 1 Muhammadiyah Primary School 2 nd Denpasar, semester 2. The subject of this research is the grade 1E by as much as 32 people. The data were analyzed using descriptive qualitative to indicate average score of achievers motivation, learning outcomes, and pass criteria minimals illustrated in graph form polygons. The results of this study showed that, the results of observation of the learning motivation research before just 60% in cycle I becomes 91% whereas the achievement of the learning post test start value rose to 69.1 61.3 (cycle-I) then up again into 80.9 on cycle – II while learning results from 40.6% to 65.6% and cycle-II being 90.6%, it can be concluded that the method of Verbal and Non Verbal Communication can be applied to increase the motivation

Kurnianingsih kurnianingsih; Annisa Eva Salsabila Anwar; Nuzul Aulya Safawi; Sabilla Aditya Prameswari

Garina 2021 Akademi Kesejahteraan Sosial Ibu Kartini Semarang

The comparation of brownies using cacao powder and cocoa powder are expected to be the standart to determine the best brownies. Purpose of the riset 1). To know the making of brownies using cacao powder and cocoa powder 2). To know the characteristic brownies using cacao powder and cocoa powder 3).To know the acceptability Panelist toward brownies using cacao and cocoa powder. Method of the riset is using literature method, documentation, experiment and hedonic test. The making of brownies cacao and cocoa experiment based on Program Study Culinary Art Academy Kesejahteraan Sosial Ibu Kartini Laboratory. Sultan Agung Street No.77 sub-distric Gajah Mungkur, Semarang. The percentage addition of cacao and cocoa powder in the making of brownies are 33% from weight of the flour. Result of the riset shown brownies with addition cacao and cocoa powder has sensory characteristics as sweet, bitter, a little bit savory, the colour is dark brown, the texture is solid and soft, the smell is like the characteristics of brownies. The acceptability of the Panelist with addition of cacao and cocoa powder in the brownies shown that the Panelist likes the brownies with cacao powder addition. Then we can conclude addition of the right choco powder for brownies is cacao powder.

U.Yuyun Triastuti; Sa’roni

Garina 2021 Akademi Kesejahteraan Sosial Ibu Kartini Semarang

Dates milk tempe is an alternative drink for health, which is made from tempe extract with natural sweetener honey and enriched with dates, so it is safe and healthy for consumption by all ages. The research objectives were to determine: 1) the organoleptic quality of date palm tempe milk; 2) community acceptance of date palm tempe milk; 3) determine the content of carbohydrates, protein and fat. The research method of making date palm milk is carried out in accordance with the procedure for making tempe milk. Organoleptic tests to determine acceptance were carried out on 30 semi-trained and untrained panelists. The nutritional content of date palm tempeh is calculated using the Gizipro application. The results showed that date palm milk has sensory characteristics of sweet and savory taste, aroma of soybean tempeh and dates, thick texture and dark brown color. Contains 5.31 g carbohydrates; 0.11 g protein; 0.14 g fat; and 2.14 mg of calcium. It has low carbohydrates, low fat content compared to tempeh milk, so it can be drunk by all ages and can prevent degenerative diseases.

Tri Rettagung Diana; Deddy Mushthafainl Akhyar

Garina 2021 Akademi Kesejahteraan Sosial Ibu Kartini Semarang

The use of young jackfruit which has low prices and abundant production is only as a basic ingredient for making traditional foods. Considering the benefits and nutritional content of young jackfruit, it is necessary to innovate the manufacture of young jackfruit cream soup with fusion techniques. The research objectives are 1) to know the process of making young jackfruit cream soup using fusion technique; 2) knowing the formula and thickening agent for young jackfruit cream soup; 3) find out the community's acceptance of young jackfruit soup cream. The experiment was carried out three times by making cream soup with 3 different thickeners, namely wheat flour with a roux system, pumpkin and potatoes. The experiment was carried out to obtain the best formula. The experimental results show how to make young jackfruit with fusion food processing techniques, starting with material selection, weighing ingredients, boiling young jackfruit, making spices, sauteing spices, boiling all ingredients, sorting bones, thickening with, wheat flour/ pumpkin/potatoes and blending. soup, filter soup. The right formula for young jackfruit cream soup is young jackfruit 120 g, pumpkin thickener 40 g(531)/roux flour 20 gr (351)/ potato 40 gr (253), 50 ml liquid milk, 2 g salt, 10 g onion, 4 g granulated sugar, 5 g margarine, 500 cc water and 140 g claws. The three experimental products were tested hedonic by 10 semi-trained people and code 531 was preferred because it has the characteristics of creamy soup with a creamy texture, savory taste, light brown color, and has the aroma of young jackfruit mixed with milk.

Ariyana Damayanti; Irmatul Afizah; Rizka Puspa Sari; Sindy Aulia Putri

Garina 2021 Akademi Kesejahteraan Sosial Ibu Kartini Semarang

The use of Fiber Optic as a light emitter in Cocktail Dress is expected to be a new innovation in the development of party fashion. The objectives of this study are: 1) To explain the process of making a cocktail dress using Fiber Optic as a light emitter. The method used in this research is the Research and Development (R & D) method. The R&D method is a research method that tests a product to produce the best product by conducting direct trials. In addition to using the method (R&D), it also uses the method of literature, documentation, and experiments. The experiment of making Cocktail Dress using Fiber Optic as a light emitter was carried out at the Laboratory of the Fashion Design Study Program of the Kartini Academy of Social Welfare, st. Sultan Agung, No. 77 Gajah Mungkur Village, Semarang City. The percentage of additional ingredients in making Cocktail Dress, namely: 3 meters Bridal Satin Fabric, 1meter Furing, 2 meters Trikod, 1 seed thread, zipper size 50 cm 1 item, zippers size 25 cm 1 item, Fiber Optic in amount of 0.75 mm-400 m. The results of this study indicate that the Fiber Optic Cocktail Dress has the characteristic that the resulted cocktail dress can shine with the light emitting from the Fiber Optic. Thus, it can be concluded that there is an opportunity for innovation from Cocktail Dress by adding Fiber Optic.

Agus Susanti; Alvi Rizqia; Martina Dwi; Rafika Suryawati

Garina 2021 Akademi Kesejahteraan Sosial Ibu Kartini Semarang

Dry lips are caused by the effect of the epidermis tissue of the lips both in cold and hot temperatures. The lip scrub moisturizes and nourishes the lips, making them look fuller, healthier, and hydrated. This study aims 1) to determine and explain the process of making Sugar and Honey Scrub. 2) To find out how to use Sugar and Honey Scrub on dry lips. 3) To know the changes of dry lips after doing Sugar and Honey Scrub. The method used in this research is experimental research, literature, documentation and interviews. The experiment was carried out at the Laboratory of the Cosmetology Study Program of the Kartini Academy of Social Welfare, Jalan Sultan Agung, No.77, Gajahmungkur Village, Gajahmungkur District, Semarang City. The formula is 1) 17g sugar, 10g honey 2) 17g sugar, 7g honey 3) 11g sugar, 6g honey. Experimental research results show Sugar and Honey Scrub in the third experiment, 11g honey and 6g sugar produce pale yellow color, sugar aroma, soft texture. The public test was carried out to 30 Panelists. In the third experiment, the average color was 1.7, Aroma 2.4 and Texture 2.7. Changes After 1 time using the Sugar and Honey Scrub, the lips look moist, supple and healthier. Thus, it can be concluded that there is a new innovation opportunity, namely Sugar and Honey Scrub for treating dry lips.

Sofia Daniati; Silvia Fatriana; Ifayatul Islamiyah; Hanida Asma Nafi’ah

Garina 2021 Akademi Kesejahteraan Sosial Ibu Kartini Semarang

Telang flower is generally used as food coloring amd traditional medicine. The contents of telang flower has many benefits for humans. Also, it is hoped that the use of telang flower in the manufacture of lip balm will be a new innovation in cosmetic products. The aim of this research is to create a lip balm based on telang flower which functions to moisturize the lips. This research uses literature, ekasperiment, documentation, observation, and public teasting methods. This experiment resulted in two products with different compositions. In addition, that the two products have been tested publicly on 20 panelists ranging in age from 18 to 25 years. From 20 panelists who have tried 2 lip balm products. It was found that most pf them preferred products with a composition of 8 grams of teang flower extract, 50 grams of coconut oil, 30 grams of olive oil, 300 grams of beeswax, and 15 grams of ethanol. This can be seen from the public test average calculation score oh the favorite indicator, namely 2,5. Basade on the statemensts above, it can be concluded that the opporatunity to be developed as a new innovation in the world of cosmetics.

Elisa Rinihapsari; Arneta Syafrinelty Fita Putri; Bernadeta Hesti Widyaningrati

Jurnal Sains dan Kesehatan (JUSIKA) 2021 Universitas Muhamadiyah Manado

Ultraviolet light is commonly used as a laboratory sterilization method to complement chemical sterilization. Until now, there has been no standard reference for the use of exposure time and exposure distance that can be effectively used for sterilizing laboratory rooms. Based on this, it is necessary to conduct this research to test the effectiveness of ultraviolet irradiation in the laboratory with varying exposure times, including 0.5, 1, 1.5, and 2 hours with an irradiation distance of 1, 1.5, and 2 meters. This experimental research was conducted at the Medical Laboratory of the Mangunwijaya Catholic Polytechnic of Medical Laboratory Technology Program. Capture of airborne microbes using the settle plate method. The results showed that the percentage reduction in the number of microbes before and after irradiation was 96.30, 62.97, 85.18, and 59.26 CFU/m3, respectively, for exposure time 0.5, 1, 1.5, and 2 hours, while the percentage decrease in the number of microbes for distance 1; 1.5; and 2m is 66.67; 67.74; 64.71 CFU/m3. The conclusion was that the adequate time to reduce the number of air microbes was 0.5 hours, while the effective distance was 1.5m.

Solikhah Solikhah; Pipin Supenah; Usdiyanto Usdiyanto

Jurnal Sains dan Kesehatan (JUSIKA) 2021 Universitas Muhamadiyah Manado

Petis is made from fermented shrimp or fish that is added with sugar and salt, it looks like a paste and has a dark brown or black color. This study is a descriptive study that aims to determine the presence or absence of Salmonella sp in petis sold in Sumber Subdistrict, Cirebon Regency, to find out what percentage of petis is contaminated with Salmonella sp. POM No. 13 of 2019 concerning Maximum Limits of Microbial Contamination in Fish and Fishery Products Including Molluscs, Crustaceans and Fermented Echinoderms. The population in this study were petis sold in Sumber Cirebon District, traders collected 15 samples using the Accidental Sampling technique. The research method used is a descriptive survey, in the examination using the isolation method through culture media and biochemical tests. The research was carried out at the Microbiology Laboratory of AAK An Nasher Cirebon in April 2022. The results obtained 9 positive samples of Salmonella sp and 6 negative samples of Salmonella sp. Based on BPOM regulation No. 13 of 2019 concerning the Maximum Limit of Microbial Contamination in Processed Food, it must be negative for Salmonella per 25 grams. From these results it can be said that the petis containing Salmonella sp was 60% and the negative petis contained 40% Salmonella sp.

M. Ibnu Ubaidillah; Hery Prambudi; Oktafirani Al As; Rizal Ibrahim Aji

Jurnal Sains dan Kesehatan (JUSIKA) 2021 Universitas Muhamadiyah Manado

Laboratory examination is very important to diagnose a disease, one of which is the examination of total cholesterol to diagnose heart disease, this examination uses serum samples obtained from blood clots that have been centrifuged, various variations of freezing are carried out to accelerate the examination results ranging from blood that is immediately centrifuged and blood that is allowed to freeze for 5-10 minutes, while according to PERMENKES No. 43 of 2013 to get blood serum allowed to freeze for 20-30 minutes at room temperature which is then centrifuged. This study aims to determine the results of differences in total cholesterol levels in blood samples immediately centrifuged and blood samples that are allowed to freeze at room temperature 260C for 10 and 20 minutes before centrifugation. The method used in this study is an experimental research method, this examination uses the CHOD-PAP enzymatic colorimetric method. For data analysis using the SPSS program using the one way anova test. This study was conducted on 15 respondents obtained from students of An Nasher Health Analyst Academy Cirebon randomly. The results of the examination of total cholesterol levels in blood samples that were immediately centrifuged had an average value of 205.8 mg/dl with the lowest value of 112 mg/dl and the highest value of 314 mg/dl, while in blood samples that were allowed to freeze for 10 minutes had an average value of 188 mg/dl with the lowest value of 144 mg/dl and the highest value of 278 mg/dl, and in blood samples that were allowed to freeze for 20 minutes had an average value of 142.6 mg/dl with the lowest value of 95 mg/dl and the highest value of 186 mg/dl. The results of this study indicate that there is a significant difference in total cholesterol levels in blood samples immediately centrifuged and blood samples left to freeze at room temperature 260C for 10 and 20 minutes before centrifugation.