The problem that is the focus of this research is the problem of work environment variables, work stress and work motivation. The population of UPTD Puskesmas Campurejo employees is 54 people. Sampling technique with saturated sampling approach. The data analysis technique used in this research is the instrument test of validity and reliability, multiple linear regression test, classical assumption test and partial hypothesis testing tested by t test while the simultaneous hypothesis is tested by F test. Overall, it can be explained that the work environment does not partially affect the performance of employees at the UPTD Puskesmas Campurejo, this can be seen from the significant value of t of 0.069 > 0.05. Work stress does not have a significant effect partially on the performance of employees at the UPTD Puskesmas Campurejo, this can be seen from the significant value of t of 0.501 > 0.05. Work motivation has a partially significant effect on the performance of employees at the UPTD Puskesmas Campurejo, this can be seen from the significant value of t of 0.000 > 0.05. Simultaneously the work environment, work stress and work motivation have a significant influence on employee performance at the UPTD Puskesmas Campurejo, this can be seen from the significant value of F of 0.000 <0.05
