Inulinase (EC 3.2.1.80) is an enzyme that can hydrolyze inulin into fructose or fructo-oligosaccharides. Using the inulinase enzyme as a catalyst in making inulinase-based fructose can obtain a fructose percentage of 90-95 percent. Inulin is a linear polysaccharide (ß-2,1-linked d-fructose residue terminated by a glucose residue) that accumulates as a storage carbohydrate in plants. One natural source that can produce inulin is bananas. Bananas are a source of inulin which contains around 0.58-1.09 percent. This research used three types of bananas, including Musa X paradisiaca, Musa paradisiaca formatypica, Musa paradisiaca var sapientum. Apart from that, you can also find out the characteristics of the microbes and enzyme activity produced from the three types of bananas. The results of this research show that the three isolated types of banana are capable of producing the inulin ase enzyme which is indicated by the growth of these microbes in ISM media. ISM media (Inulinase Selective Media) is an inulinase mixing media where only inulin-producing microbes can grow on the media. The microbes isolated from the three types of bananas that had the highest inulinase enzyme activity value were Raja Nangka bananas with an enzyme activity value of 0.405 IU/mL. Meanwhile, the lowest inulinase enzyme activity value was in Kepok bananas with an enzyme activity value of 0.088 IU/mL.

The market demand for potatoes is very high, and their cultivation can affect the environmental balance, so innovation in potato yield is needed. Medium land is an area with an elevation of 300-700mdpl. Crop yields in medium land are not optimal and more susceptible to disease than highlands. The role of microorganisms in PGPR and Tricho Powder can maximize crop yields and help reduce disease intensity. This study aims to determine the effect of different concentrations of commercial PGPR in increasing the yield of potato cultivation, the effect of different concentrations of Tricho Powder in suppressing the intensity of disease attacks, and find out one species in PGPR. This research was conducted with RAL (6 treatments with 4 replications). The treatments included P0 (Farmers’ Habits), P1 (PGPR 20mL), P2 (PGPR 40 mL), P3 (Tricho Powder 80gram/10Liter), P4 (Tricho Powder 150gram/10L), and P5 (PGPR 20mL+Tricho Powder) 80gr/10L). The data was then analyzed by ANOVA. If there is significance, a follow-up test is carried out, which is determined based on the KK value. The results showed that differences in PGPR concentrations positively affected potato yields with the combination treatment of PGPR 20mL/10L+Tricho Powder 80 gr/10L (P5), which was the best treatment producing the highest mean in each parameter. In the same treatment, disease intensity was reduced by 37.55% compared to the control treatment. Based on its ability to produce IAA from 5 isolates, only 1 isolate (IS 5) showed positive results and was continued to molecular tests.