Previous research used used cooking oil as a handsoap which has a strong inhibition of bacterial growth but still causes dry and slightly itchy skin, so this research was perfected by adding olive oil as a base. The problem is, which formulations of used cooking oil and olive oil in handsoap can inhibit bacterial growth and moisturize the skin, as well as their quality against organoleptic tests, irritation, and high foam. The purpose of this study was to determine the formulation of handsoap which has strong antibacterial properties, moisturizes the skin, and determines its quality against organoleptic tests, irritation, and high foaming. The composition of used cooking oil and olive oil used was 125 gr : 125 gr (Formula 1), 150 gr : 100 gr (Formula 2), 175 gr : 75 gr (Formula 3), and 200 gr : 50 gr (Formula 4). The making of this handsoap uses an experimental method. Testing the zone of inhibition of bacterial growth used the in vitro method, organoleptic testing was done through descriptive tests, and testing for PH, Irritability, and Foam Height was through descriptive tests using observational analytic methods. The results of this study are that this handsoap has a strong ability to inhibit bacterial growth, can moisturize the skin, is not irritated, and has a lot of foam, but has a large PH which is not in accordance with SNI so that it cannot be used by the public

Tujuan penelitian ini adalah mengetahui formulasi komposisi antara minyak jelantah dan KOH yang mana dari tiga formulasi komposisi yang memiliki daya hambat pertumbuhan bakteri yang bagus di kulit. Pada Proses Pembuatan Hand Soap, menggunakan metode hot process, yang tentunya diawali dengan pemurnian minyak jelantah menggunakan arang aktif. Pengujian daya hambat pertumbuhan bakteri dengan menggunakan cemaran bakteri Staphylococcus Aureus, dan pengujan organoleptik dilakukan dengan cara menggunakan hand soap yang dihasilkan   kepada sepuluh koresponden. Hasil yang diapai dalam peneitian ini adalah Hand Soap dengan formulasi 375 gr minyak jelantah dan 125 gr KOH memiliki daya hambat pertumbuhan bakteri di kulit, nyaman digunakan, wangi, dan tidak iritasi.