Penelitian ini merupakan salah satu langkah awal pengembangan pangan di Indonesia. Hal tersebut berkaitan dengan gandum yang dibudidayakan di Indonesia, yaitu gandum varietas DWR-162. Tujuan utama penelitian ini adalah menentukan pengaruh tepung gandum utuh varietas DWR-162 terhadap kandungan pati resisten (penentuan secara enzimatis) dan kadar gizi, yang biskuit meliputi kadar air (AOAC), abu (AOAC), lemak total (AOAC), karbohidrat total (Anthrone), protein terlarut (Biuret), dan serat kasar (AOAC). Parameter gizi tersebut dibandingkan dengan Standar Nasional Indonesia (SNI) 01-2973-1992 tentang mutu dan cara uji biskuit. Hasil penelitian ini menunjukkan bahwa biskuit dengan 10-50% tepung gandum utuh memiliki kadar air, abu dan lemak total berturut-turut adalah 0,85-0,92%, 1,35-1,43%, dan 33,44-41,70%. Nilai tersebut memenuhi syarat mutu SNI. Selain itu, kadar protein terlarut biskuit adalah 30,41-45,62%. Namun, karbohidrat total biskuit lebih rendah dari SNI, yaitu 37,58-49,32% dan kadar serat kasarnya lebih tinggi dari SNI yaitu 3,91-5,73%. Pati resisten biskuit meningkat hingga kadar substitusi gandum utuh 50% dari 17,8% menjadi 30,19%. Tingginya pati resisten biskuit gandum lokal ini sesuai dengan nilai indeks glikemik biskuit yang tergolong rendah, yaitu < 55. Dengan demikian, gandum varietas DWR-162 berpotensi menjadi alternative bahan pangan fungsional.This study was one of the initial steps in the development of Indonesian food based onlocal wheat, namely wheat DWR-162. The primary objective of this study was to determine the effect of the whole-wheat flour on the resistant starch content (enzimatically determined) and nutrition content of biscuits i.e moisture content (AOAC), ash (AOAC), total fat (AOAC), total carbohydrate (Anthrone), soluble protein (Biuret), and crude fiber (AOAC). The nutritional values were compared to the Indonesian National Standard (SNI) 01-2973-1992 about quality of biscuit. The results of this study showed that biscuits with 10-50% whole wheat flour had moisture, ash and total fat contents of 0.85% to 0.92%, 1.35% to 1.43%, and 33.44% to 41.70%, respectively. These values met the standard. In addition, the soluble protein content of biscuit was 30.41% to 45.62%. However, the total carbohydrate content, 37.58% to 49.32% was lower than SNI,while the crude fiber content, 3.91% to 5.73% was higher than SNI. Resistant starch increased from 17.8% to 30.19% along with the increased whole wheat substitution from 0% to 50%. The high content of resistant starch was in line with the low glycemic index of the biscuits, < 55. Thus, whole wheat DWR-162 could be potentially employed as a functional food ingredient.  

Tujuan dari penelitian adalah menentukan pengaruh penambahan tepung belut pada proses pembuatan tempe terhadap kadar protein, lemak, dan asam lemak tempe yang dihasilkan. Data kadar protein dan lemak dianalisis menggunakan Rancangan Acak Kelompok dengan 5 perlakuan dan 6 kali pengulangan dan dilanjutkan dengan Uji Beda Nyata Jujur dengan tingkat kebermaknaan 5%. Komposisi asam lemak dianalisis dengan kromatografi gas yang terhubung dengan spektrofotometer massa. Tempe juga diuji sensorik oleh 15 panelis berdasarkan uji kesukaan. Kadar protein dan kadar lemak tempe meningkat seiring dengan penambahan tepung belut dari 0% sampai 7,5%, yaitu 22,20% hingga 32,31% dan 8,53% hingga 17,53% berturut-turut. Akan tetapi, peningkatan kadar penambahan tepung belut belum tentu meningkatkan kesukaan panelis terhadap tempe yang dihasilkan dan disajikan dalam bentuk digoreng. Uji sensorik menunjukkan bahwa tempe dengan 3% penambahan tepung belut adalah tempe yang paling disukai, dengan perolehan skor 3,87; 4,20; 3,93; dan 4,40 berturut-turut untuk parameter tesktur, aroma, rasa, dan kenampakan, di mana skor 3, 4, dan 5 secara berurutan berarti netral, disukai, dan sangat disukai. Komposisi asam lemak dalam tempe belut 0%, 3%, dan 7,5% berturut-turut adalah 14,28%; 17,22%; 12,36% asam heksadekanoat, 37,56%; 43,67%; 36,19% asam linoleat, 32,96%; 33,05%; 34,94% asam oktadekenoat, dan 3,00%; 3,87%; 2% asam oktadekanoat. Dengan demikian, penambahan tepung belut sebanyak 7,5% mampu meningkatkan kandungan asam lemak tak jenuh dan menurunkan kandungan asam lemak jenuh.The purpose of this research was to determine the effect of eels flour addition in tempeh on protein content, fat content and fatty acid content of tempeh. The protein and fat contents were analyzed using Randomized Completely Block Design (RCBD) with 5 treatments (0; 3; 4.5; 6; and 7.5% eels flour additions) and 6 replications. The differences among means of every treatment were calculated by using Honestly Significant Difference (HSD) with 5% level of significance. The fatty acidcomposition wasdetermined by Gas Chromatography-Mass Spectrophotometer (GC-MS). Tempeh was assessed using organoleptic test with 15 panelists to find out which tempeh was favored. The protein and fat contents of tempeh increased along with 0% to 7.5%eels flour addition; they were, respectively, 22.20 to 32.31% and 8.53 to 17.53%. Tempeh with 3% eels flour addition was most favored. The organoleptic scores for tempeh with 3% eels flour addition were 3.87 for texture, 4.20 for smell, 3.93 for taste, and 4.40 for appearance, in which the scores of 3, 4, and 5 meant neutral, liked, and very liked, respectively. The fatty acid compositions in tempeh with 0%, 3% and 7.5% eels flour addition were 14.28%, 17.22%, and 12.36% hexadecanoic acid consecutively, 37.56%, 43.67%, and 36.19% linoleic acid concecutively, 32.96%, 33.05%, and 34.94% octadec-16-enoic acid consecutively, and 3.00%, 3.87%, and 2% octadecanoic acid consecutively. The addition of eels flour as much as 7.5% could increase the unsaturated fatty acids content and lower the saturated fatty acids content.