Health workers are 1.5 times higher risk of work accidents compared to other professions, with 90% of incidents occurring in developing countries. WHO noted that 3 million out of 35 million health workers are exposed to blood pathogens, increasing the risk of HIV (0.4%), HBV (27-37%), and HCV (3-10%). One of Health center accreditation purpose is to makesure safety to both health workers and patient. In 2023, out of 37 health centers in Semarang, 36 health centers (97.3%) achieved “Plenary” accreditation and 1 Health Center (2.7%) achieved “Main” accreditation. The purpous of this study are to To determine the current OSH practice and determine OSH aspect that needs improvement based on Health Ministry Regulation No.52 of 2018. qualitative methods with a case study design. Occupational Safety and Health (OSH) management at Padangsari Health Center was evaluated based on 92 indicators from three main aspects. In the OHS management criteria, 14 out of 23 indicators (60.9%) have been met. For OSH standards, 45 out of 61 indicators (73.8%) have been met. Meanwhile, 8 indicators related to OSH training, reporting, and recording have all been met. Although Padangsari Health Center has achieved "Main" accreditation with an achievement of 72.8%, further efforts are still needed to meet at least 80% of the assessment indicators in order to obtain the highest accreditation, namely "Plenary". It is recommended to improve indicators, especially in improving OHS planning, strengthening supervision, ensuring staff compliance with SOPs, and evaluating the effectiveness of OSH implementation in the future.