Organizational culture is a collection of values, rules, and beliefs held by members of an organization, influencing the way they think and act in achieving goals. This study aims to describe the basic concepts, roles, impacts, and challenges in implementing organizational culture in educational institutions. The type of research is a literature study. Data were collected from accredited national books and journals using Google Scholar and PoP, with an analysis of 15 articles filtered into 5 main articles. The results of this study (1) Organizational culture in educational institutions contributes to creating a conducive work environment, increasing the professionalism of educators, and strengthening work discipline, (2) A strong culture can improve performance, collaboration, and adaptability, while a culture that is not in line with the vision and mission can cause disorientation and (3) Challenges in implementing organizational culture include resistance to change, understanding organizational values, differences in understanding between members and technological adaptability.