This research aims to know the knowledge and attitude of pregnant mothers about hepatitis in the Siompu District, South Buton District. The research uses descriptive qualitative design using thematic analysis, A semi-structured one-on-one interview through a face-to-face interview at Siompu. The interviews are recorded and transcribed word by word and then analyzed thematically. A total of 5 pregnant women participated in this study with a response rate of 93.7%, and each interview lasted 20 to 40 minutes. Five thematic categories were identified, while the participant’s psychological experiences were classified into five stages, namely (1) perception: the presence of anxiety; (2) attitudes: fears, and doubts about viruses; (3) transitional periods: complex and diverse psychological feelings; (4) adaptation period: self-adaptation and assistance from external support; and (5) reflection period: reflection on life and nature. The results study showed that the perception in pregnant women of hepatitis infection is still less, while pregnant mothers showed a very positive attitude towards therapy and immunization. So, it is highly recommended to raise awareness about the hepatitis virus, the ways of transmission, and possible treatment options.