Determination of total flavonoid levels is calculated based on the linear regression equation based on the calibration curve of the quercetin standard solution from the reading results of the UV-Vis spectrophotometry tool. The absorbance value (ppm) is entered into the linear regression formula as the y value, while the x value is the concentration of flavonoids in the working sample solution. The results are stated in triplicate and the flavonoid content is stated with the equivalence of flavonoid standards using quercetin standard standards, qualitative and quantitative research methods to determine metabolite compounds and determination of levels using UV-vis spectrophotometry. Qualitative testing is carried out by taking a thick extract and adding reagents according to the identified compounds. Based on the results of the tests carried out, it shows that alkaloid, flavonoid, steroid, triterpenoid, tannin, and saponin compounds show positive results while quinone compounds show negative results. Quantitative testing From the results of this study, the total flavonoid content of the ethanol extract of itching leaves (Laportea stimulans) was 175.961%. Based on the results of qualitative tests, it shows that the ethanol extract of itchy leaves (Laportea stimulants) positively contains several compounds including alkaloids, flavonoids, steroids, terpenoids, tannins, and saponins which are indicated by changes in color. Based on the results of quantitative tests, it shows that the determination of the content of 95% ethanol extract of itchy leaves (Laportea stimulants) spectrophotometrically at a wavelength of 430 nm has an average total flavonoid content of 0.2999%.
 

Cacing laut (Perinereis albuhitensis) sangat berpotensi sebagai bahan obat antibakteri, antifungi baru karena memeliki kandungan metabolit sekunder yaitu : Saponin dan Flavanoid, terpenoid, alkalaod tanin yang bekerja sebagai anti bakteri dan antifungi. Tujuan daripenelitian ini adalah untuk mengidentifikasi daya hambat antibakteri ekstrak cacing laut (Perinereis albuhitensis) yang diekstraksi menggunakan pelarut etanol 96% terhadappertumbuhan bakteri Staphylococcua aureus Pengujian aktivitas antibakteri ini menggunakan metode difusi agar dengan menggunakan beberapa konsentrasi 1,5%, 10%, 20%, dan 30%. Kontrol positif menggunakan Amoxicillin dan kontrol negatif yaitu aquadest steril. Hasil dari penelitian ini menunjukan bahwa ekstraksi cacing laut menggunakan etanol 96% mampu menghambat pertumbuhan bakteri Staphylococcus aureus pada konsentrasi 10%, 20%, dan 30%.

Fragrant pandanus is a type of monocot plant from the pandanaceae family. Fragrant pandan leaves have various ingredients and can be used as antibacterial ingredients and are safe to use, which have been known empirically by the Indonesian people. There are many benefits to the fragrant pandan leaf plant (Pandanus amaryllifolius Roxb), namely as an analgesic (toothache medicine) and antibacterial. The purpose of this study was to determine the chemical content, formulation and stability test in mouthwash preparations. In this study, the maceration method is used which is used to filter simplicia containing chemical components that are easily soluble in liquid. The results of this study indicate that in the phytochemical screening test there are chemical compounds which include flavonoids, alkaloids, saponins, and tannins, which are thought to have contributed to bacterial activity. Research has also been carried out on the formulation and stability test of mouthwash preparations from the ethanol extract of pandan fragrant  leaves  (Pandanus  amaryllifolius roxb)  where  the  results  show  that  the  formulation  can  be  used  as mouthwash of fragrant pandan leaves (Pandanus amaryllifolius roxb) with different concentrations in formulation I. as much as 15.5%, in formulation II as much as 16.5%, and in formulation III 17.5%. And the physical quality test of the mouthwash includes an organoleptic test which has a distinctive smell of fragrant pandan leaves and menthol and the pH test has a stable pH of 5. The results obtained basically produce mouthwash with good physical quality.