The study investigated how Cola nitida affects the sweat concentrations of sodium (Na), potassium (K), and chloride (Cl) in male and female subjects. Sixty healthy participants (30 males, 30 females) aged 18–28 years, with varying body weights (underweight, normal weight, overweight), were selected. Subjects were exposed to three conditions: normal chamber temperature (27°C, 70% RH), raised temperature (37°C, 90% RH), and exercise at normal temperature (27°C, 70% RH) while riding a bicycle ergometer at 750J/min for 20 minutes. Prior to the experiment, 0.5g/kg body weight of Cola nitida was consumed, followed by a 90-minute rest. Sweat samples were collected to measure ion concentrations. Results showed no significant changes in sodium (Na) concentrations between males and females under normal and raised temperature conditions. However, under exercise conditions, there was a significant increase in sweat Na in female underweight subjects compared to males. Regarding chloride (Cl), there was no significant difference between males and females under all conditions. For potassium (K), significant increases were observed in the sweat of female underweight subjects compared to males in normal, raised temperature, and exercise conditions. Conversely, decreases in sweat potassium were noted in female normal weight subjects under normal conditions and overweight subjects under raised temperature and exercise conditions compared to males.

How Cola nitida could affect the urine calcium and magnesium concentrations of the male human subjects in comparison to the female counterpart were focused on. Sixty subjects (males=30 and females=30) and unaccustomed to Cola nut eating were involved. Their age range was between 18 and 28years. Three (3) sub-categories were involved: underweight=10, normal weight=10 and overweight=10. Hypertensive, renal and any subject with cardio-pulmonary-related conditions were exempted from the study. The normal chamber temperature, (RT=270C and RH=70%), the raised chamber temperature (RT =370C and RH=90%) were studied. Sequel to the foregoing, the subjects sat quietly in the sweat chamber for 20 minutes. The third experimental condition was the normal chamber temperature with exercise (RT=270C and RH=70%) and the subjects rode a bicycle ergometer at 750J/minute for 20 minutes. All of these happened prior to the consumption Cola nitida. Ahead of the experimentation in the sweat chamber, the subject urinated in a container in order for the timed urine volume to be measured and urine was again taken at the end of the experimentation that was also measured in order to obtain the actual urine volume within the given period. Thereafter, the urinary flow rate was calculated. The urine calcium and magnesium concentrations were likewise estimated. 0.5g/kg body weight of Cola nitida was given to each subject and chewed as a bolus. After swallowing, 50ml of deionized water was given to each subject to drink and the subject was allowed to rest for 90 minutes before being brought into the sweat chamber. The results showed that in respect to urine Ca ions concentration, no significant change was observed in a comparison between the males and females after the ingestion of Cola nitida under normal, raised temperature and exercise experimental conditions. As regards urine Mg ions concentration, no significant change was observed in a comparison between the males and females after the ingestion of Cola nitida under normal and exercise experimental conditions. However, there was a significant increase in Mg ions concentration in the female UW and NW subjects compared to the males after the consumption of Cola nitida under raised temperature condition.