This study aims to determine the effect of adding EM4 probiotics in various doses to commercial feed on the growth of jatimbulan tilapia (Oreochromis niloticus L). Probiotics are known to play a role in increasing digestive efficiency, nutrient absorption, and maintaining the health of the fish's digestive tract, so they are expected to support optimal growth. The research method used was a Completely Randomized Design (CRD) with four treatments, namely: P0 (control/without probiotics), P1 (5 ml EM4/kg feed), P2 (7 ml EM4/kg feed), and P3 (11 ml EM4/kg feed), each with four replications. The parameters observed were the growth in length and weight of jatimbulan tilapia during the maintenance period. The results showed that the administration of EM4 probiotics at certain doses was able to provide a significant effect on the growth of tilapia compared to the control treatment. Treatment P2 (7 ml/kg feed) provided the best growth results compared to other doses, both in terms of increasing length and body weight. Meanwhile, administering probiotic doses that are too low (P1) or too high (P3) tends to be less effective in supporting optimal growth. This is thought to be due to the disruption of the microbial balance in the digestive tract when the probiotic dosage is incorrect. This research provides useful information for Jatimbulan tilapia cultivation practices, particularly regarding the use of probiotics to increase growth efficiency. Using the correct probiotic dosage can be a strategy to increase the productivity and sustainability of freshwater fish farming.
 

Indonesia's biodiversity holds great potential for drug development, including through the use of traditional medicinal plants such as tapak liman (Elephantopus scaber L.), suruhan (Peperomia pellucida L. Kunth), and temu ireng (Curcuma aeruginosa Roxb.). These plants have been used in traditional medicine and are known to contain bioactive compounds with potential anticancer properties. Tapak liman contains compounds capable of inducing apoptosis in cancer cells. Suruhan is known to have cytotoxic and immunomodulatory effects, while temu ireng contains compounds with antioxidant and anticancer activity. This study aims to identify and quantify the bioactive compounds in these three plants using Gas Chromatography-Mass Spectrometry (GC-MS) and to explore their therapeutic potential in cancer treatment. The results of GC-MS analysis successfully identified six main compounds that have potential as anticancer agents, namely Phytol, Caryophyllene, Apiol, Germacrone, Germacrene B, and one additional compound. Temu ireng contains Germacrone, Germacrene B, and Caryophyllene, while suruhan contains Caryophyllene, Apiol, and Phytol. Meanwhile, tapak liman shows the presence of bioactive compounds that support cytotoxic activity. The focus of this study is on the interaction of bioactive compounds with the Bcl-2 protein, which plays a role in inhibiting apoptosis in cancer cells. Additionally, molecular analysis revealed that Germacrone, Germacrene B, Caryophyllene, and Apiol can also bind to the p53 protein, known as a guardian of the genome and a trigger for apoptosis. These findings suggest that the three plants have high potential as natural sources of anticancer agents. Further in vitro and in vivo studies are recommended to test the biological efficacy of these compounds in the context of cancer therapy, thereby bridging traditional knowledge with modern scientific approaches in the development of natural-based medications.

Technological advancements emphasize the importance of artificial intelligence (AI) literacy for younger generations. However, junior high school students in Sinar Bhakti Village still lack basic knowledge of AI. This community service program aims to introduce the fundamental concepts of AI to junior high school students through an interactive educational approach. The method employed involved practice-based workshops using visual media and simple simulations. The activity was met with high enthusiasm from the students, especially during simulations related to the application of AI in daily life. The results showed an improvement in students' understanding of AI concepts, as reflected in their ability to articulate the materials taught during the program. Furthermore, this program provided students with new insights into the potential of future technologies and created an active space for discussions on AI applications in various fields.