Drinking water is a basic human need that must meet health requirements to be suitable for direct consumption without causing health risks. Drinkable water must be free from various contaminants, both physical, chemical, and microbiological that can harm the human body. One of the sources of drinking water that is widely used by the community is refillable drinking water (DAMIU), which is now growing due to its affordable price and ease of access. However, the water quality from DAMIU is often a concern due to potential contamination due to sanitation that does not meet standards. This study aims to analyze the sanitary conditions of the refillable drinking water depot (DAMIU) and assess the quality of drinking water based on physical, chemical, and microbiological parameters in the working area of the Punti Kayu Health Center, Palembang. A total of 15 DAMIU are the object of research that are examined through laboratory tests and direct observation of the sanitary conditions and equipment of the depot. The results showed that all DAMIU (100%) had met the general sanitation requirements, such as room cleanliness, equipment, and workers. However, microbiological testing found that 33.4% of the water samples contained E. coli and Coliform bacteria, indicating the presence of contamination. In addition, all samples (100%) did not meet the ideal drinking water pH requirements, and as many as 40% of the samples had aluminum content that exceeded the threshold set by the Indonesian Minister of Health. From these findings, it can be concluded that although the sanitary conditions of DAMIU are relatively good, the quality of the drinking water produced still requires serious attention.