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In the fruit processing industry, peel is one of the main by-products. Non-edible portions of fruit are likewise seen as waste and are frequently thrown out into the environment. Nowadays, one of the main sources used by the pharmaceutical sector to isolate and extract secondary metabolites is fruit peels. Many applications known for lime peel, especially for antibacterial. This study focuses on the isolation, chemical composition characterization, and antibacterial activity on skin pathogenic bacteria of lime peel essential oil (LPEO). LPEO were isolated using steam distillation and characterized by GC/MS. A total of 24 components were identified. The major constituents of the LPEO were d-limonene (40.24%), β-terpinene (15.68%), sabinene (13.15%), citral (3.41%), α-Terpineol (3.40%), and terpinene-4-ol (2.94%). The antibacterial activity was determined using the Kirby-Baurer method against several bacteria that can cause skin infections. The antibacterial activity of LPEO against Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis dan Propionicbacterium acne is confirmed. LPEO showed varied levels of antibacteria activity against the gram positive and gram negative bacteria. Zone of inhibition (ZOI) in millimetres was used to measure the antibacterial activity, and amoxicillin was used as a control.  

Plants have several ways to protect themselves from bacteria, one of which is by producing compounds that are toxic or repellent to bacteria. Compounds produced by plants are known as secondary metabolite compounds which originate from secondary metabolic processes. Several secondary metabolite compounds such as flavonoids, alkaloids, saponins, tannins, steroids and terpenoids are currently widely used as antibacterials. One of the plants that has the potential to be developed as a herbal medicine is kaffir lime leaves. Citrus hystrix is ​​the part of the plant most often used by the public. Both for cooking and traditional medicine purposes. In this research, it is hoped that lime peel extract can act as an antibacterial for Escherichia coli and staphylococcus aereus, so that it can be used as a herbal plant for diseases caused by these two bacteria. In the research, the concentrations used for each test bacteria were 50%; 25%; 12.5%. in observing S. aureus bacteria 1x24 hours with a concentration of 50%, the size of the inhibitory zone was 16 mm, the 25% concentration was 11 mm and the 12.5% ​​concentration was 9 mm. Meanwhile, E. Coli bacteria did not show any reaction to the heruk peel extract.

Plants have several ways to protect themselves from bacteria, one of which is by producing compounds that are toxic or repellent to bacteria. Compounds produced by plants are known as secondary metabolite compounds which originate from secondary metabolic processes. Several secondary metabolite compounds such as flavonoids, alkaloids, saponins, tannins, steroids and terpenoids are currently widely used as antibacterials. One of the plants that has the potential to be developed as a herbal medicine is kaffir lime leaves. Citrus hystrix is ​​the part of the plant most often used by the public. Both for cooking and traditional medicine purposes. In this research, it is hoped that lime peel extract can act as an antibacterial for Escherichia coli and staphylococcus aereus, so that it can be used as a herbal plant for diseases caused by these two bacteria. In the research, the concentrations used for each test bacteria were 50%; 25%; 12.5%. in observing S. aureus bacteria 1x24 hours with a concentration of 50%, the size of the inhibitory zone was 16 mm, the 25% concentration was 11 mm and the 12.5% ​​concentration was 9 mm. Meanwhile, E. Coli bacteria did not show any reaction to the heruk peel extract.