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Cassava tapai is a traditional Indonesian food product produced through the fermentation of cassava (Manihot esculenta) with the help of microorganisms, particularly the yeast Saccharomyces cerevisiae. This product not only has cultural and economic value but also contains bioactive compounds with health benefits, such as probiotics and fermentation metabolites. However, the quality of cassava tapai is significantly influenced by process factors, particularly incubation time. This study aimed to analyze microbial growth and changes in the chemical properties of cassava tapai with varying fermentation times. The study was conducted using an experimental design with fermentation times of 24, 48, and 72 hours at room temperature. The main parameters observed included the number of microbial colonies (cfu/g), pH changes, and alcohol content produced during the fermentation process. The results showed that microbial growth increased significantly, peaking at 48 hours, with the highest colony count compared to other treatments. After 72 hours, the number of colonies decreased, likely due to ethanol accumulation and decreased substrate availability, which reduced microbial activity. The pH value tended to decrease with increasing fermentation time, reflecting the formation of organic acids during the process. Meanwhile, the alcohol content showed an increasing trend from the beginning to the end of fermentation, although the growth rate was relatively slower at 72 hours. These findings confirm that varying incubation length significantly influences microbial dynamics and chemical changes in cassava tapai. The optimal fermentation time is around 48 hours, as this is the phase where the balance between microbial growth, alcohol formation, and sensory characteristics is maintained. The results of this study can serve as a basis for developing standards for cassava tapai production at both household and industrial scales, while also strengthening efforts to preserve traditional foods with a modern scientific approach.

Fish bekasam is one of Indonesia's traditional fermented products that involves the activity of microorganisms, especially lactic acid bacteria (LAB), to produce unique organoleptic, chemical, and microbiological characteristics. The fermentation process of bekasam plays a significant role not only in extending the shelf life of fish but also in creating a distinctive sour taste and texture that differs from other fish products. This study aims to analyze the microbiological community involved in the fish bekasam fermentation process through observation and identification of bacteria using Safranin Violet and Iodine staining techniques. This study employs a literature review approach, examining various references related to the fermentation process, the dominant bacteria species, and the environmental factors that affect the quality of bekasam. The analysis revealed that lactic acid bacteria such as Lactobacillus plantarum, Lactobacillus casei, and Pediococcus spp. play an important role in the fermentation of bekasam fish. These bacteria produce lactic acid, which contributes to the sour taste and plays a role in forming the product's texture. The Safranin Violet and Iodine staining techniques were effective for identifying bacteria based on their cell wall characteristics, with Gram-positive bacteria dominating the fermentation process. Environmental factors such as the fermentation time, salt concentration, and the addition of ingredients like carbohydrates or turmeric extract influence the dynamics of bacterial populations in the fermentation of bekasam. This study also emphasizes the importance of controlling pathogenic bacterial contamination, such as Salmonella spp. and Escherichia coli, to ensure food safety in bekasam products. The findings provide a deeper understanding of the microbiological dynamics in fish bekasam fermentation and the relevance of staining techniques in microbiological analysis, which can be used to improve the quality and safety of traditional fermented products. This study also opens opportunities for developing safer and higher-quality bekasam products.

Kombucha is one of the processed products of fermented sugar and tea with a starter mixture ofkombucha culture called SCOBY (Symbiotic Cultures of Bacteria and Yeast). Kombuchafermentation is carried out using yeast and bacteria microorganisms. Pomegranate or often calledpomegranate is a fruit that has quite high antioxidants. The purpose of this study was to determinethe characteristics of pomegranate juice kombucha with variations in concentration andfermentation time which have high antioxidant activity. This study used a factorial randomizeddesign with two factors and two repetitions. The factors used were variations in the percentage ofpomegranate juice (30%, 40%, and 50%) and fermentation time (7 days, 9 days, and 11 days). Thebest chemical and organoleptic analysis based on the highest antioxidant activity was 50%pomegranate juice treatment and 9 days of fermentation time with 71,10% antioxidant activity,total phenol 9,04 GAE/L, reducing sugar 2,45%, total acid 1,35%, pH 3,1 , anthocyanin 53.52%,lightness (L*) 44.72 , redness (a*) 5.15, yellowness (b*) 2.57, fruity aroma 2.27, sour taste 2,85, andfor overall preference 3,49 for the treatment of adding 50% pomegranate juice and 9 days offermentation time. Pomegranate extract able to improve activity of antioxidant for kombucha. 

The addition of probiotics such as Lactobacillus sp to fermented feed is expected to improve nutrient digestibility, suppress the growth of pathogenic microorganisms, and improve feed conversion efficiency, thereby promoting optimal shrimp growth. This study used an experimental method on vannamei shrimp doc 80 using a complete randomized design (RAL) with four treatments: P0 (feed without fermentation as a control), P1 (feed fermentation for 0 hours 10ml/100g with Lactobacillus sp), P2 (feed fermentation for 24 hours with 10ml/100g Lactobacillus sp), P3 (feed fermentation for 48 hours 10ml/100g Lactobacillus sp), P3 (feed fermentation for 72 hours 10ml/100g Lactobacillus sp) by doing 6 repetitions which were carried out for 1 month/30 days in the maintenance tub. The results showed that Treatment A without fermentation gave absolute growth with an average of 1 gr, Treatment B with no fermentation gave absolute growth with an average of 1.89 grams, Treatment C with a fermentation time of 48 hours gave absolute weight growth with an average of 2.15 grams, Treatment D with no fermentation gave absolute growth with an average of 0.74 grams. Meanwhile, the water quality parameters during the study were obtained with water temperature ranging from 28.3-29.6o C, pH ranging from 7.8-8.0, and dissolved oxygen ranging from 4.03-4.66 mg/l. With the anova test, the absolute weight growth showed that with different fermentation time differences, there was a real effect on the growth of vannamei shrimp in C treatment, which was 2.15 gr. And in the results of the anova test, the four water quality parameters were not different between the treatments and were in the optimal range in the life of vannamei shrimp. Based on this study, it can be suggested that the optimal fermented feed is in the time range of 48 hours and there is further research for doc 80 and above.

Kombucha tea is a drink produced through a fermentation process of a mixture of tea and sugar using kombucha culture or also called SCOOBY ( Symbiotic Culture of Bacteria and Yeast) . The basic ingredients for making kombucha tea are granulated sugar solution and tea bags, in this study it was varied using fruit skin. Vitamin C is known as an important antioxidant found in many fruits, including in rujak fruit skin which is often considered waste. The purpose of this study was to determine the levels of vitamin C in kombucha tea fermented from fruit skin waste. This study used qualitative and quantitative methods. The qualitative method uses the Benedict test and the quantitative method uses the calculation of the percentage of vitamin C content using the iodimetric titration method. The results of the qualitative analysis of the Benedict test were positive for vitamin C with a change in the color of the sample solution to green-yellowish and the analysis of the percentage of vitamin C content obtained a result of 2.3124% or equal to 0.23124 mg / 10 mL of sample solution. The vitamin C content of kombucha tea can vary depending on the base ingredients used, fermentation time, and storage temperature.

Donuts are foods made from high protein wheat flour, in this study donuts were made from substitution of wheat flour and soybean flour. This study aims to determine the effect of soy flour substitution (Glycine max L.) and of determine fermentation time of (60, 75 and 90 min) on soy flour donuts which have the highest protein content and panelists' favorite. Complete Rendomized Design (RAL) Faktorial with two factors used in this research. The first  factor was is the ratio of  wheat flour and soy flour  (95;5, 90;10, 85;15) . The second  factor was is the variation of fermentation time (60, 75,  90 min). It was determined for protein, ash , water, fat , reducing sugar, total carbohydrates, and organoleptic. The data was determined Analysis using of Variance (ANOVA). The results showed that donuts with the addition of soy flour and fermentation time had a significant effect (α<0.05) on protein content, sugar content, flavor and overall preference. The best treatment of soybean flour donuts based on chemical and organoleptic tests, namely the ratio of wheat flour and soybean flour (85:15) using 90 minutes of fermentation. Soy flour donuts with the best treatment had a 20.30% of moisture; 1.77% ash content; protein content 10.38%; reducing sugar content 22.52%; total carbohydrates 49.51%; color dark brown 3.25; unpleasant flavor 2.95; savory taste 3.01 and very liked overall favorite 3,57.

Banana peel is banana processing waste that can be used as poultry feed, but because of its high crude fiber content, it is necessary to do processing to reduce it, one way is by processing processes such as fermentation. The fermentation process takes time to get good nutritional results. This study aims to determine the content of crude protein, crude fiber and crude fat of banana peels fermented with SOC at different times. This study used an experimental method with a Complete Randomized Design with three treatments and six repeats. The three treatments are W0= (0 hours), W1= 24-hour fermentation, and W2= 72-hour fermentation. The observation parameters are crude protein content, crude fiber, and crude fat.  The results showed that the SOC Fermented Kepok banana peel nutrition showed the highest crude protein analysis results were P2 with an average crude protein of 22.14% and differed very markedly from P1 and P0, with the lowest crude protein average at P0: 10.20%. The results of the analysis of the highest crude fiber content were P0 with an average crude fiber of 22.38% and differed very significantly from P1 and P2 with the lowest average crude fiber at P2: 15.30%. The highest crude fat content analysis was P2 with an average crude fat of 16.67% and differed markedly from P1 and P0, with the lowest average crude fat at P0; 13,71%. Fermentation of kapok banana peel with SOC for 72 hours produced 22.14% protein, 15.30 crude fiber, and 16.67% crude fat.