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This study examines the bioethical and Sharia dilemmas arising from the production of slaughter-free meat through animal cell culture as a modern food innovation. Concerns regarding the halal status of the cell source, the use of culture media such as Foetal Bovine Serum, and ethical issues related to animal welfare necessitate an in-depth analysis of the halal status and moral implications of this technology. The research method employs a literature study with a descriptive qualitative approach, reviewing journals, books, and contemporary fatwas related to cultured meat, bioethics, and Islamic law. The results indicate that the Sharia aspect heavily depends on the cell source, the medium used, and the culture process, while the bioethical perspective highlights animal welfare, scientific transparency, and the moral responsibility of researchers. Furthermore, the acceptance among Muslim communities is significantly influenced by trust in halal certification bodies and the availability of transparent information. This study affirms the need for biotechnology-based halal standardization and the development of fully halal media and supporting materials to ensure the widespread acceptance of cultured meat in the future.

Rapid global population growth has led to climate change and agricultural land degradation, posing significant challenges to the stability of global food security. The traditional livestock sector is known to absorb high levels of greenhouse gas emissions that can accelerate natural resource degradation and require innovative and sustainable approaches to meet the demand for animal protein. This study aims to analyze the contribution of cultured meat technology as an ethical and environmentally friendly alternative to improving global food security. This study uses a desk study method that addresses the technical, social, economic, and ethical aspects of cultured meat production. The analysis results indicate that the application of this technology has the potential to reduce land use and carbon emissions by up to 70% compared to conventional livestock systems, in addition to reducing animal suffering and the risk of zoonotic disease transmission. However, its application still faces several obstacles, such as high production costs, dependence on whey-based culture media, and difficulties in halal certification and public acceptance. These findings underscore the importance of synergy between scientists, governments, and religious authorities to ensure the safety, effectiveness, and equivalence of technology with ethical principles and socio-cultural values. With strong regulatory support and ongoing research, cultured meat technology has the potential to be a strategic solution for building a resilient, equitable, and environmentally responsible global food system.

This study aims to explore the potential of cassava extract (Manihot esculenta)-based growth media for isolating microorganisms from soil. The use of alternative culture media is crucial, especially in regions with limited access to commercial media such as Nutrient Agar or Potato Dextrose Agar, which are relatively expensive. Cassava, a tropical tuber rich in starch and widely available in Indonesia, offers significant promise as a natural substrate for microbial growth. Microbial isolation was performed using a serial dilution method on soil samples collected from an organic garden. The media was prepared from cassava juice mixed with agar and glucose, sterilized, and poured into petri dishes. After inoculation, microbes were incubated for seven days at room temperature. Colony morphology was observed macroscopically, and Gram staining was used to identify bacterial characteristics. The results revealed that cassava extract media effectively supported the growth of diverse microbial colonies, varying in shape, color, and edge structure. Most isolates were identified as Gram-positive, with rod and coccus forms. The presence of pigmented colonies indicates the potential of certain isolates to produce bioactive compounds. In conclusion, cassava extract-based media is a viable alternative for soil microbiology studies. It is not only cost-effective but also facilitates the exploration of local microbial diversity with potential applications in biotechnology, including bioremediation and enzyme production. This approach encourages the use of indigenous resources in scientific research, promoting sustainability and accessibility in microbiological practices.

This study aims to identify the microscopic structure of fungi growing in Manihot esculenta (cassava) waste using Gram A (crystal violet) staining. Cassava waste is a type of organic waste that has the potential to serve as a growth medium for various microorganisms, including fungi. Microscopic observations were conducted to determine the morphology of fungal cells that grow naturally in the waste. The Gram staining method was used to facilitate visualization of the fungal cell wall structure, with an emphasis on crystal violet staining as the primary dye. Waste samples were fermented for 3–5 days to allow fungal growth, then taken and microscopic slides were made. The results showed the presence of purplish-purple fungal hyphae and spores, indicating that the fungal cells were able to retain the crystal violet stain. This structure indicates that the type of fungus growing has a thick cell wall and is likely included in the Ascomycota or Zygomycota group. This identification demonstrates the potential use of Manihot esculenta waste as a substrate for microbiological studies and the development of fungal-based biotechnology. This study also emphasizes the importance of simple staining techniques in assisting the initial identification of microorganisms in organic media.

The agribusiness sector plays a crucial role in the economy, especially in developing countries like Indonesia. However, traditional challenges such as limited land, climate change, and conventional farming practices often hamper productivity. Integration of technology in agribusiness offers innovative solutions to overcome these constraints and improve efficiency and yields. This study uses a descriptive qualitative approach with a literature study research design that focuses and aims to identify and analyze effective technology-based agribusiness development strategies to increase productivity sustainably. By exploring various technologies such as precision agriculture, Internet of Things (IoT), artificial intelligence (AI), and biotechnology, and their implementation in increasing productivity in various agribusiness subsectors. This study discusses the challenges and opportunities in technology adoption in the agribusiness sector, and recommends policies and strategies to encourage digital transformation in agriculture.

The interaction between genetic and environmental factors plays a crucial role in determining phenotypic traits in organisms. This study aims to analyze these interactions using computational approaches, including statistical models and machine learning algorithms. The data used include genetic factors (genotypes) and simulated environmental factors. Results indicate that machine learning models such as Random Forest can detect interaction patterns with high accuracy, as demonstrated by significant R² values. Additionally, heatmap visualizations provide deeper insights into the non-linear effects of genetic-environment interactions. This study highlights the potential of computational methods in exploring complex interactions, with broad applications in health, agriculture, and biotechnology.

This study aims to determine the feasibility and impact of virtual laboratories in learning the material of innovation in biological technology (biotechnology) for high school students in grade X. The feasibility assessment was carried out by three expert validators using a questionnaire instrument that measured aspects of material, media, and language and based on readability tests by teachers and students. The results of the study showed that the percentage of validity of the Virtual Laboratory from material experts was 95%, media experts 83%, language experts 90% with the third category being "Very Feasible". The results of the readability test from teachers obtained a percentage of 96% while the results of the readability test from students obtained a percentage of 99%. These results indicate that the Virtual Laboratory is Feasible and Practical to be used for biology learning, especially biotechnology material. The results of the teacher response test on the use of Virtual Laboratory showed a percentage of 95% while the results of the student response test were 75%. The results of the T test showed that Virtual Laboratory had a significant effect on students' conceptual understanding with a significance of 0.001. These results indicate that Virtual Laboratory is effective in improving students' conceptual understanding, especially in biotechnology material.

Cases of dengue fever (DBD) in Solo, including in Gajahan Village, have increased quite significantly. Until the beginning of the year, there were 1,010 cases of DBD recorded throughout Central Java, with 34 cases resulting in death. The purpose of this Community Service Priority problems in this caseconsist of two aspects, including health, law, Health problems include how the community can prevent the spread of Dengue Fever (DBD). While in terms of Law, it discusses community protection related to Aedes Biotechnology with Wolbachia in Handling Dengue Fever. The method of implementing the community service with a pre-test, then the presentation of material on "Education of Aedes Biotechnology with Wolbachia in Handling Dengue Fever: Health Law Study" is carried out offline, with lecture methods, demonstrations, and Q&A discussions. At the end of the material presentation session, a demonstration of making anti-mosquito spray products will be held and ended with a question andanswer session and post-test. The results of the Community Service show that there is a significant difference in the amount of income, the amount of pretest scores before receiving material exposure and the posttest scores after receiving the material.

Agrotechnology plays a crucial role in increasing agricultural productivity through the implementation of modern innovations and technologies. Challenges such as land degradation, climate change, and limited natural resources can be addressed through precision technology, smart irrigation, biotechnology, and agricultural mechanization. This study employs a literature review method by analyzing various studies from 2019 to 2024 that discuss the impact of agrotechnology on agricultural yields. The findings indicate that the application of precision irrigation systems can enhance water use efficiency by up to 40%, while the use of genetically engineered superior crop varieties can increase yields and improve plant resistance to pests and diseases. Additionally, the utilization of digital technology, such as the Internet of Things (IoT), enables more efficient and data-driven land management. However, major challenges in implementing these technologies include limited farmer access, high investment costs, and low digital literacy among farmers. Therefore, policy support, financial incentives, and farmer training are necessary to ensure the widespread and sustainable adoption of technology. Thus, agrotechnology can serve as a primary solution for increasing agricultural productivity while maintaining environmental sustainability.

The agriculture sector is facing unprecedented challenges as a result of climate change. As temperatures continue to rise and weather patterns change, the dynamics of plant-virus interactions change significantly, requiring innovative solutions to ensure global food security. This review article examines the relationship between climate change and the prevalence and severity of cucumber virus diseases, their impact on cucumber yield and quality, and subsequent economic implications, with a focus on the critical pathogen cucumber mosaic virus (CMV). The method in this journal article uses library research, which is a method with data collection by understanding and studying theories from various literatures related to the research. The results showed that as climate change increases, it is imperative to investigate its impact on cucumber virus management, specifically how increasing temperatures and changing rainfall patterns may affect virus epidemiology and disease resistance mechanisms. In addition, exploring integrated pest management strategies that combine traditional breeding with modern biotechnology advances is essential. The application of RNA-based technologies presents a promising avenue for effective virus control, potentially reducing the economic burden on cucumber farmers.

The oil and gas sector, particularly the upstream segment involving exploration and production, plays a crucial role in contributing to Indonesia's economy. Indramayu Province, being one of the country’s oil and gas-producing areas, also experiences both direct and indirect effects from upstream oil and gas operations. This study aims to assess the influence of upstream oil and gas activities on the local economy in Indramayu Province. The research employs both qualitative and quantitative methods, incorporating secondary data analysis and interviews with relevant stakeholders. Findings indicate that upstream oil and gas operations have a substantial impact on the regional economy, notably through job creation, improved income levels, and effects on other economic sectors. However, challenges remain regarding the sustainability of natural resources and the need to better optimize the benefits for local communities. Keywords: Upstream Oil and Gas Operations, Local Economy, Indramayu Province, Economic Impact, Resource Sustainability, High-quality Oil

This research was carried out for approximately 1 month from 17 January – 05 March 2022. In situ measurements included temperature (Suhu), acidity (pH), and dissolve oxygen (DO). Directly in the Lab. Aquaculture Biotechnology Fakulty of Agriculture University of Muhammadiyah Sukabumi. the test included testing the ex situ water quality at the IPB University Laboratory. Testing ammonia and alkalinity. The results of the above objectives show a comparison of the growth of water quality in the vertiqua system (Vertical Aquaculture), to determine the effectiveness of the vertiqua system in goldfish cultivation on growth, feeding effeciency, and water quality.  

In the current era of technological development, the application of conventional biotechnology can be seen in making tempeh, the manufacturing process of which uses fermented soybeans as raw materials with the help of microorganisms in the form of yeast. This tempe making training activity was held on April 20 2024 at Johar Market with a total of 29 participants. The method used in this training is to use the method of explaining the material and practicing directly. Based on the results of the training activities, there was an increase in participants' knowledge and abilities, this increase could be seen when the results of the questionnaire were distributed before and after the training was carried out. All participants know that tempeh is one of the foods most liked by the majority of Indonesian people. The speaker provided knowledge and experience regarding tempeh processing, when the participants already understood about tempeh processing, after that they carried out direct practice, up to the washing stage and entered the manufacturing stage. In this training, participants understand that tempeh has a fairly high source of vegetable protein and this training provides benefits and increases interest in opening up tempeh business opportunities.

The waste piles in the landfill pose a higher environmental hazard compared to the waste at its source due to the potential formation of leachate and air emissions. Consequently, waste management from the source is essential to minimize the amount of waste in the landfill. The waste management methods at each location typically vary based on the composition, characteristics, and quantity of waste present in that specific area. In this study, an analysis of waste piles at facility X was conducted through the process of sorting and weighing the waste during October 2023, with the objective of providing research findings that can be utilized to determine suitable waste management options for the location. The research revealed that the dominating composition of the waste consists of organic waste, with garden waste accounting for 55.95% and food residue comprising 10.75%. Composting was selected as the recommended alternative for waste treatment in the area. Garden waste, primarily composed of dry leaves, is suggested to be treated using the pile composting method, while food residue is suggested to be treated through a combination of Black Soldier Fly larvae biotechnology and Takakura composting.

Pharmaceutical biotechnology products in the form of hand washing soap with an active ingredient of telang flower kombucha fermented solution have the potential to inhibit the growth of Staphylococcus capitis, Bacillus cereus, and Pantoea dispersa bacteria. The aim of this study was to formulate and prepare hand washing soap with an active ingredient in a solution of seagrass kombucha fermentation to inhibit the growth of Staphylococcus capitis, Bacillus cereus, and Pantoea dispersa. This research is an experimental laboratory by making 4 hand washing soap preparations, which consist of a hand washing soap base without an active substance. Hand washing soap base added to a solution of fermented butterfly pea flower kombucha at a sugar concentration of 20%, 30%, and 40%. Disc diffusion is a method used to calculate the average diameter of the inhibition zone for the three tested bacterial growth. The research data obtained were analyzed through one way ANOVA and post hoc analysis. The results of the ANOVA test proved that the obtained p value <0.05, which means that there were significant differences in the three treatment groups in inhibiting the growth of the three test bacteria. The post hoc analysis results also proved that 40% concentration was the best concentration in inhibiting the growth of the three tested bacteria, namely 18.34 mm (S. capitis/strong), 16.39 mm (B. cereus/strong), and 13.33 mm (P. disperse/strong).

Telang flower has the potential to inhibit the growth of Candida albicans, Malasezia furfur, Pitosporum ovale, and Aspergilus fumigatus through the kombucha fermentation biotechnology method. The purpose of this study was to determine the antifungal activity of telang flower kombucha at various concentrations of white sugar. The varying concentrations of granulated sugar used in this study were 20%, 30%, and 40% (w/v), each repeated 3 times. The positive control used was kombucha made from green tea. The negative control used was sterile distilled water. The well diffusion method is one of the methods used to test antifungal activity. The fementation of telang flower kombucha has overall antifungal activity. White sugar concentration of 40% kombucha telang flower was the best concentration in inhibiting the growth of fungi as a whole when compared to the concentration of white sugar kombucha telang flower of 20%, 30% and positive and negative controls.