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Health issues among the elderly are often related to declining bodily functions, digestive disorders, and specific nutritional needs for easily digestible foods. The 60-and-older age group is at high risk for gastrointestinal disorders due to low fiber intake. Therefore, alternative foods are needed that are soft in texture, neutral in taste, safe, and nutritious. One potential solution is the development of food products based on tempeh gembus with the addition of banana hearts, which are rich in fiber, soft in texture, and readily available. This study employed an experimental method using a Completely Randomized Design (CRD) to test three formulation variations (F1, F2, F3). The nutritional content of formulation F1 includes energy 111.575 kcal, carbohydrates 20.185 g, protein 6.91 g, fat 0.355 g, and fiber 6.67 g. Formula F2 contains 88.225 kcal of energy, 14.095 g of carbohydrates, 7.72 g of protein, 0.205 g of fat, 7.3 g of fiber, and 0.0015% flavonoids. Formula F3 has 107.015 kcal of energy, 7.995 g of protein, 0.295 g of fat, 6.88 g of fiber, and 0.0021% flavonoids. In conclusion, the best tempeh with added banana hearts is Formula F2, with its nutritional content, fiber, flavonoids, and BAL >10⁸ CFU/g, which has the potential to serve as a functional food and probiotic source to help improve digestive health in the elderly. Further testing is recommended directly on elderly groups, along with product shelf-life testing.

Biofloc technology is a super-intensive fish cultivation method, which benefits heterotrophic bacteria to harvest organic chemical compounds and amines from fish metabolic residues to be converted into bacterial proteins. The use of probiotics is very important to be carried out in cultivation activities, Probiotics are living microorganisms that have a beneficial effect on humans by balancing the microflora in their intestines. The bacteria in probiotics have a mechanism in forming several enzymes for feed digestion. This study aims to determine the effect of administering Pro Top probiotics with different doses on the absolute weight growth of tilapia (Oreochromis niloticus). Probiotics are mixed with feed with a biofloc cultivation system. The method used in this study is an experimental method with a Completely Randomized Design (CRD) with 4 treatments and 6 replications, namely by adding different doses to the treatment. Treatment A (0.5 grams / kg), B (1 gram / kg), C (2 grams / kg) and D (5 grams / kg). The parameters observed are absolute weight growth. Supporting parameters for air quality are temperature, pH, and DO. Data were analyzed using the ANOVA test. Based on the results of the study, it shows that administering different doses of Pro Top probiotics has a significant effect on the absolute weight growth of tilapia (Oreochromis niloticus). Treatment A (0.5 grams/kg) gave the lowest absolute weight growth of 31.05 grams and treatment D (5 grams/kg) gave the highest absolute weight growth of 40.30 grams. Air quality parameters including air temperature ranged from 25.3 – 26.1°C, acidity level around 7.5 – 8.0. And dissolved oxygen ranged from 4.7 – 5.3 ppm. The results of the analysis of variance (ANOVA) of the three air quality parameters showed no difference between the treatments of tilapia (Oreochromis niloticus).  

This study aims to analyze the implementation of health education as an integrated marketing strategy for probiotic products through social media at PT Yakult Indonesia Persada. Health education is increasingly recognized as a strategic approach in value-based marketing, where companies offer not only products but also knowledge and added value for consumer well-being. The research employed a qualitative descriptive method using field observation, interviews with marketing staff, and a literature review of related studies. The findings indicate that PT Yakult Indonesia Persada successfully incorporates health education into its marketing activities through the Yakult Lady program, school visits, health seminars, factory tours, and interactive digital content on social media platforms. These strategies have proven effective in raising consumer awareness of digestive health, strengthening brand image, and maintaining customer loyalty. The study highlights the crucial role of social media integration in reaching younger demographics, fostering engagement, and amplifying educational messages. The consistent delivery of educational content, supported by visual storytelling and personalized approaches, has contributed significantly to Yakult’s sustainable growth in the Indonesian probiotic market. Theoretically, this study enriches the discourse on digital marketing and health communication, while practically, it offers insights for companies seeking to align promotional strategies with consumer education and public health improvement.

Bekasam is a traditional fermented food made from snakehead fish (Channa striata) through spontaneous fermentation with the addition of salt and rice over several days. This fermentation process promotes the growth of indigenous microorganisms, particularly lactic acid bacteria (LAB), which play a crucial role in developing distinctive flavors and potentially providing health benefits. LAB are well-known for their probiotic effects, such as maintaining gut microbiota balance and enhancing immune system function. This study aimed to identify the presence of LAB in bekasam using the Gram staining method. Samples were taken from bekasam that had been fermented for three days. Bacterial isolation was conducted by culturing the samples on appropriate media, followed by Gram staining to observe bacterial morphology and cell wall characteristics under a microscope. Observations revealed a predominance of Gram-positive, rod-shaped (bacillus) bacteria, which are the primary morphological characteristics of LAB. This morphology is consistent with bacterial groups such as Lactobacillus spp., which are commonly found in traditional fermented products. These findings strengthen the assumption that snakehead fish–based bekasam is not only a traditional food source with rich flavors but also has potential as a natural probiotic source. This potential opens opportunities for bekasam to be developed as a functional food that supports digestive health and immune function. Further research is recommended to perform molecular identification to accurately determine bacterial species and to evaluate their probiotic activity in vivo. Thus, bekasam holds not only cultural and culinary value but also added value in the field of health and the functional food industry.

This study aims to determine the effect of adding EM4 probiotics in various doses to commercial feed on the growth of jatimbulan tilapia (Oreochromis niloticus L). Probiotics are known to play a role in increasing digestive efficiency, nutrient absorption, and maintaining the health of the fish's digestive tract, so they are expected to support optimal growth. The research method used was a Completely Randomized Design (CRD) with four treatments, namely: P0 (control/without probiotics), P1 (5 ml EM4/kg feed), P2 (7 ml EM4/kg feed), and P3 (11 ml EM4/kg feed), each with four replications. The parameters observed were the growth in length and weight of jatimbulan tilapia during the maintenance period. The results showed that the administration of EM4 probiotics at certain doses was able to provide a significant effect on the growth of tilapia compared to the control treatment. Treatment P2 (7 ml/kg feed) provided the best growth results compared to other doses, both in terms of increasing length and body weight. Meanwhile, administering probiotic doses that are too low (P1) or too high (P3) tends to be less effective in supporting optimal growth. This is thought to be due to the disruption of the microbial balance in the digestive tract when the probiotic dosage is incorrect. This research provides useful information for Jatimbulan tilapia cultivation practices, particularly regarding the use of probiotics to increase growth efficiency. Using the correct probiotic dosage can be a strategy to increase the productivity and sustainability of freshwater fish farming.  

Cell-based therapy is an innovative approach currently experiencing rapid development in the biomedical field, particularly in the areas of immunotherapy and gene therapy. This approach utilizes living cells, such as genetically modified T cells, to recognize and destroy abnormal cells such as cancer cells or cells with genetic mutations. The development of gene engineering technologies, such as CRISPR/Cas9, has enabled scientists to precisely edit damaged genes, thereby repairing or restoring normal cell function. This study aims to examine recent advances in cell-based therapy, focusing on cell modification techniques, the efficiency of the immune system in targeting cancer cells, and the integration of gene therapy technology and immune cells. Furthermore, this study also identifies several challenges that remain, including the risk of side effects due to undesirable immune reactions, the genetic stability of the modified cells, and the long-term sustainability or viability of transplanted cells. While these challenges remain serious concerns, several clinical studies have shown that cell-based therapy has significant potential in the treatment of difficult-to-treat diseases, such as advanced cancer and rare genetic disorders. The results of this study demonstrate that cell-based therapy not only offers a new approach to treatment but also opens the door to more personalized and precise therapies. However, for broader implementation in clinical practice, further, in-depth research is needed regarding safety, long-term efficacy, and strict regulation. This research is expected to make a significant contribution to the development of more effective and safe future therapies.

Probiotics from West Sumatra curd have the potential to improve the production performance of local West Sumatra ducks, where Probiotics are live bacteria given as food sulpementation. Giving probiotics can have a beneficial effect on health where probiotics produce inhibitor compounds so that they can maximize the absorption of food in the intestines of livestock. The purpose of this study was to observe the production performance of ducks given probiotics. The design used was a randomized complete factorial method. The first factor (A) Dosage of probiotics (LAB) is with treatment A1: 1 ml (12.7 x 108 CFU/g); A2: 2 ml (25.4 x 108 CFU/g); A3: 3 ml (38.1 x 108 CFU/g). The second factor (B) is the frequency of probiotic administration, namely B1: 0 times; B2: 1 time; B3: 2 times; B4: 3 times; B5: 4 times; B6: 5 times; B7: 6 times, consisting of 4 replicates with a total of 346 ducks. The observed variables were food consumption, body weight gain, ration conversion, carcass percentage. The results of this study were the average food consumption at a dose of 3 ml with 5 times administration (995.79g). The highest average body weight gain (369.74 grams) dose of 1 ml and 5 times administration. The highest carcass percentage with a dose of 3 ml and a frequency of 5 times (72.63%).

This study aimed to evaluate the effect of varying honey concentrations on the antioxidant activity, chemical properties, and organoleptic quality of a probiotic kombucha beverage prepared from Clitoria ternatea L. The research was motivated by increasing exposure to free radicals due to environmental pollution and the demand for alternative natural antioxidants. Kombucha was produced with honey concentrations of 10%, 20%, and 30% (w/v), and analyses were performed using the DPPH assay for antioxidant activity, measurements of pH, total acidity, and total phenolic content for chemical properties, as well as sensory evaluations by trained panelists. The results showed that kombucha with 10% honey exhibited the lowest IC₅₀ value, indicating the highest antioxidant activity, while higher concentrations increased the total phenolic content without significantly enhancing the effective antioxidant potential. These findings suggest that excessive honey addition does not necessarily improve the functional benefits of the product, indicating the need for further formulation optimization to develop a high-quality, marketable probiotic beverage.

One of the problems of catfish cultivation (Clarias gariepinus) that can hinder this cultivation business is the slow growth of fish weight. The purpose of this study is to determine the effect of different administration of Mina Pro probiotics on the absolute weight growth of pearl catfish seeds measuring 5-7 cm in the maintenance tub. The method used in this study is an experimental method. The design used in this study was a Complete Randomized Design (RAL) with four treatments and six replicates. The treatment given is: addition of probiotics mina pro at a dose of 2 ml/kg of feed, 4 ml/kg of feed, 6 ml/kg of feed and without the addition of probiotics to feed. Other parameters that were also studied included water quality including temperature, pH and DO.

Giving probiotics such as probiotic boster on feed greatly helps the growth of absolute weight in Catfish seeds because the good bacteria contained in these probiotics help facilitate catfish seeds in digesting the feed so as to increase the efficiency of the feed. This study aims to see how effective the probiotic administration is. The study was conducted in Kramas District, Gianyar regency, Bali Province for 30 days using experimental methods, using a complete randomized design with a total of 4 treatments and 6 repetitions. Treatment A is by giving 3 ml of probiotic boster every 1 kg of feed. Treatment B by giving 5 ml probiotic boster every 1 kg of feed. Treatment C by giving 7 ml probiotic boster every 1 kg of feed. Treatment D by giving 9 ml probiotic boster every 1 kg of feed. In this study showed significantly different results on the addition of probiotic boster with different doses on the growth of absolute weight of catfish fry aged 1 month-2 months. Treatment D gave the highest result with an absolute weight gain of 55.33 grams, and treatment a showed the lowest result with an absolute weight gain of 25.17 grams. Water quality Data during the study obtained water temperature ranges from 27-29 oC, acidity ranges from 7-8, and dissolved oxygen ranges from 3.34 to 4.26 ppm. The results of the analysis of variance (ANOVA) of the three parameters of water quality there is no difference between the treatment and the life range of catfish fry (Clarias sp.)

Vannamei shrimp is one of the shrimp species with economic value and is one of the national leading commodities. On the other hand, one of the problems in vannamei shrimp cultivation is its slow growth, slow growth is caused by the less optimal absorption of nutrients in the feed. In dealing with these problems, one of the things that is done is the administration of probiotics. The addition of probiotics such as Biolacto containing Lactobacillus sp bacteria to the feed can increase the appetite of shrimp. This study aims to determine the effect of biolacto probiotics on feed on the growth of the absolute weight of vannamei shrimp. This study used a Complete Random Design (RAL) with four treatments: A (feed without a mixture of probiotics), B (probiotics 2 gr/100 gr feed), C (probiotics 4 gr/100 gr feed), and D (probiotics 6 gr/100 gr feed). Treatment B with a concentration of probiotics 2gr/100g feed gave the highest absolute weight growth of 9.2 gr. Water quality data during the study were obtained with water temperature ranging from 28.5 – 29.2°Celsius, salinity ranging from 36 – 37 ppt, pH ranging from 7.5 – 8.0, and dissolved oxygen ranging from 4.0 to 4.63 mg/l. The results of the ANOVA analysis of the four water quality parameters showed no difference between treatments and were in the optimal range of life of vannamei shrimp (Litopenaeus vannamei).

The use of probiotics is very important in cultivation activities. Probiotics are live microorganisms that have a beneficial effect on the host by balancing the microflora in their intestines. In increasing the nutritional content of artificial feed (pellets), the bacteria in probiotics have a mechanism for forming several enzymes for digesting feed. This study aims to determine the effect of giving delacto probiotics at different doses in artificial feed on the absolute weight growth of tilapia (Oreochromis niloticus). Probiotics are mixed with feed using a biofloc cultivation system. The method used in this research was an experimental method with a Completely Randomized Design (CRD) with 4 treatments and 6 replications, namely by adding different doses to the treatments. Treatments A (8ml/kg), B (16 ml/kg), C (24 ml/kg) and D (32 ml/kg). The parameter observed was absolute weight growth. Water quality supporting parameters include temperature, pH and DO. Data were analyzed using the ANOVA test. Based on the research results, it shows that giving different doses of delacto probiotics has a significant effect on the absolute weight growth of tilapia (Oreochromis niloticus). Treatment A (8 ml/kg) gave the lowest absolute weight growth of 32.83 grams and treatment D (32 ml/kg) gave the highest absolute weight growth of 40.57 grams. Water quality parameters include water temperature ranging from 25.3 – 26.1֯C, acidity ranging from 7.5 – 8.0. And dissolved oxygen ranges from 4.7 – 5.3 ppm. The results of analysis of variance (ANOVA) for the three water quality parameters showed no differences between treatments (Oreochromis niloticus) fry.

The addition of probiotics such as Lactobacillus sp to fermented feed is expected to improve nutrient digestibility, suppress the growth of pathogenic microorganisms, and improve feed conversion efficiency, thereby promoting optimal shrimp growth. This study used an experimental method on vannamei shrimp doc 80 using a complete randomized design (RAL) with four treatments: P0 (feed without fermentation as a control), P1 (feed fermentation for 0 hours 10ml/100g with Lactobacillus sp), P2 (feed fermentation for 24 hours with 10ml/100g Lactobacillus sp), P3 (feed fermentation for 48 hours 10ml/100g Lactobacillus sp), P3 (feed fermentation for 72 hours 10ml/100g Lactobacillus sp) by doing 6 repetitions which were carried out for 1 month/30 days in the maintenance tub. The results showed that Treatment A without fermentation gave absolute growth with an average of 1 gr, Treatment B with no fermentation gave absolute growth with an average of 1.89 grams, Treatment C with a fermentation time of 48 hours gave absolute weight growth with an average of 2.15 grams, Treatment D with no fermentation gave absolute growth with an average of 0.74 grams. Meanwhile, the water quality parameters during the study were obtained with water temperature ranging from 28.3-29.6o C, pH ranging from 7.8-8.0, and dissolved oxygen ranging from 4.03-4.66 mg/l. With the anova test, the absolute weight growth showed that with different fermentation time differences, there was a real effect on the growth of vannamei shrimp in C treatment, which was 2.15 gr. And in the results of the anova test, the four water quality parameters were not different between the treatments and were in the optimal range in the life of vannamei shrimp. Based on this study, it can be suggested that the optimal fermented feed is in the time range of 48 hours and there is further research for doc 80 and above.

This article discusses a literature review on the benefits of probiotic-containing foods for health. Fermented foods are food products processed with the help of microorganisms or enzymes, producing components beneficial to human health. In Indonesia, fermented foods such as tempeh, tapai, green tea, pickled vegetables, and bekasam are known for their high health value and potential to gain international recognition. This literature study aims to analyze the benefits of probiotics in fermented foods, particularly regarding digestive health, immunity, and disease prevention.  Probiotics such as Lactobacillus plantarum, Lactobacillus curvatus, and Lactobacillus GG, as well as indigenous yeasts like Saccharomyces cerevisiae and Candida tropicalis, play a crucial role in the fermentation of traditional Indonesian foods. These microorganisms help improve gut microbiota balance, inhibit the growth of pathogenic microbes, and produce beneficial bioactive compounds. Research indicates that probiotics can enhance immune responses, reduce inflammation, and improve glucose metabolism and lipid profiles.  The findings of this study underscore the importance of fermented foods as a public health strategy, considering their contributions to supporting digestive function, strengthening immunity, and preventing various diseases. Traditional Indonesian fermented foods not only hold cultural value but also have significant potential as functional foods to improve quality of life.

Tofu has become one of the most popular foods among the people of Indonesia. This encourages the existence of a growing tofu manufacturing industry. However, the growth of this industry also results in a lot of waste being generated. If the waste is not treated properly, it can cause pollution because it contains very high BOD and COD levels. One of the ways that researchers do is by utilizing local probiotic bacteria to help reduce BOD and COD levels. Data analysis in this study is in the form of statistical analysis, where statistical data will be presented with graph/diagram methods that show the results of differences in BOD and COD values in Tofu Industry Wastewater before and after treatment. Based on the results of the study, there was a significant decrease in BOD and COD levels due to the addition of local probiotic bacteria. The efficiency of the largest decrease in BOD levels was 79.88% in the sample variation with a probiotic content of 75% when the incubation time interval was 32 hours. Meanwhile, the largest decrease in COD levels also occurred in the same sample variation with an efficiency of 74.76%.    

This study aims to analyze the impact of administering commercial probiotic bacteria on the wastewater from tofu industries on changes in Bio-logical Oxygen Demand (BOD) and Dissolved Oxygen (DO) levels. Tofu industry wastewater is known to have high BOD and low DO levels, which pose a potential environmental pollution risk. This study used a pure experimental method with variables including the ratio of tofu industry wastewater to commercial probiotics (1:1, 1:2, 1:3) with an incubation interval of 8 hours. The results showed that the administration of commercial probiotic bacteria significantly reduced BOD levels and increased DO levels in the wastewater. The 1:3 ratio of tofu industry wastewater to commercial probiotics showed the most optimal effect in increasing DO and decreasing BOD. An incubation time of 32 hours showed the most optimal effect in increasing DO and decreasing BOD. The 1:3 volume ratio between tofu wastewater and probiotics (25% tofu wastewater and 75% probiotics) proved to be the most effective, with the highest DO increase of 2.51 mg/L and the largest BOD decrease of 144.20 mg/L. In addition, incubation time also had a significant effect, the longer the incubation time, the more optimal the probiotic activity in reducing BOD levels and increasing DO levels. The conclusion of this study is that the administration of commercial probiotic bacteria at a 1:3 ratio and an incubation time of 32 hours is an effective way to improve the quality of tofu industry wastewater.

Indonesia boasts a number of tofu processing facilities totaling, around 84,000 both scale and large scale operations. These facilities collectively consume 2.56 million tons of soybeans each year. The high volume of tofu production poses a threat to the environment due to the generation of waste. If this waste is directly released into water bodies it can alter the waters characteristics potentially harming life. To address this issue Tofu Industry Wastewater can be implemented by introducing probiotics containing microorganisms that aid in breaking down matter and other components in the waste. This study focuses on analyzing bacterial colonies in Tofu Industry Wastewater by examining bacterial colonies present in these products. Various mixtures of Tofu Industry Wastewater and commercial probiotics will be tested at ratios of 1:1, 1:2, 1:3, and 1:4 over time periods ranging from 24, 48, 72, 96, 120, and 144 hours. The findings indicate that incorporating probiotics into Tofu Industry Wastewater can lead to a reduction in pollution levels.

The tofu industry in Indonesia generally utilizes simple processing technologies, leading to unresolved wastewater management issues. Tofu wastewater contains high organic matter, measured by BOD, a parameter indicating the oxygen required by microorganisms to decompose organic matter. High BOD can decrease dissolved oxygen (DO) levels in water, which is crucial for aquatic life. This study investigates the effectiveness of local probiotics in reducing BOD and increasing DO in tofu wastewater (ABIT). Three treatments were applied: pure ABIT, neutralized ABIT (ABIT-N), and ABIT mixed with local probiotics in three ratios (1:1, 1:2, and 1:3). DO was tested at 0, 8, 16, 24, and 36 hours, while BOD was tested at 0 and 5 days. The results demonstrate that local probiotics can effectively reduce BOD and enhance DO levels in ABIT. The highest DO (3.36 mg/L) was observed in ABIT-N mixed with local probiotics at a 1:3 ratio after 32 hours. The most significant BOD reduction (135.77 mg/L) was also achieved at the 1:3 ratio. These findings suggest that local probiotics offer an eco-friendly approach to treating tofu wastewater.  

Environmental pollution has become a serious problem in the world, including in Indonesia. Tofu waste is waste from processing soybeans and consists of solid and liquid waste. Solid waste in the form of dirt left over from cleaning is usually called tofu dregs, while liquid waste is water resulting from washing tofu. Industry knows that disposing of waste directly into water bodies can pollute those waters, because it can lower the pH of the air and affect aquatic biota. Therefore, the industry knows that it must carry out processing first to prevent environmental problems. In this study, researchers used commercial probiotic bacteria to reduce BOD and COD levels in tofu wastewater. The research was carried out by testing in the laboratory and using statistical data with graphic methods which can provide a comparison of the water content of tofu waste before and after it was given. The action given in the research was a ratio of 1:1, 1:2 and 1:3 water to tofu waste with commercial probiotic bacteria. Apart from that, researchers also added incubation times within each 8 hour interval, namely at 0 hours, 8 hours, 16 hours, 24 hours and 32 hours. The results of the research show that administering commercial probiotics can reduce BOD and COD levels in tofu wastewater. The most significant reduction occurred in a ratio of 1:3 in COD and BOD to 242.69 mg/lt and 148.00 mg/lt with an incubation time of 32 hours. This proves the commercial effect of probiotic bacteria to reduce BOD and COD levels so that it can help reduce environmental pollution, especially in the air.      

Tujuan  pengabdian masyarakat adalah pengenalan metode dan teknologi tepat guna  dengan metode akuamber  untuk teknik memadukan  usaha bertanam sayur mayur  dengan budidaya ikan dalam wadah ember.  Pelaksanaan  kegiatan di lahan  sempit, pekarangan  dan masih terkena sinar matahari karena tanaman sayuran dan ikan membutuhkan sinar matahari.   Hasil usaha dapat dimanfaatkan konsumsi, peningkatan gizi bagi Balita dan anak  serta anggota keluarga yang lain. Bahkan dapat dikonsumsi bersama dalam kegiatan rukun tetangga (RT/RW) dan kegiatan PKK sebagai usaha swadaya masyarakat.  Keberlanjutan kegiatan  PPM kepada  kelompok sasaran  dapat meningkatkan ketahanan pangan keluarga dengan menerapkan aplikasi probiotik pada budidaya polikultur antara ikan dan tanaman sayuran skala rumah tangga.