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Dwi Nurul Qomariah; Dea Amanda Caressa; Elita Endah Mawarni

Jurnal Ilmu Kesehatan dan Gizi 2026 Pusat Riset dan Inovasi Nasional

Health issues among the elderly are often related to declining bodily functions, digestive disorders, and specific nutritional needs for easily digestible foods. The 60-and-older age group is at high risk for gastrointestinal disorders due to low fiber intake. Therefore, alternative foods are needed that are soft in texture, neutral in taste, safe, and nutritious. One potential solution is the development of food products based on tempeh gembus with the addition of banana hearts, which are rich in fiber, soft in texture, and readily available. This study employed an experimental method using a Completely Randomized Design (CRD) to test three formulation variations (F1, F2, F3). The nutritional content of formulation F1 includes energy 111.575 kcal, carbohydrates 20.185 g, protein 6.91 g, fat 0.355 g, and fiber 6.67 g. Formula F2 contains 88.225 kcal of energy, 14.095 g of carbohydrates, 7.72 g of protein, 0.205 g of fat, 7.3 g of fiber, and 0.0015% flavonoids. Formula F3 has 107.015 kcal of energy, 7.995 g of protein, 0.295 g of fat, 6.88 g of fiber, and 0.0021% flavonoids. In conclusion, the best tempeh with added banana hearts is Formula F2, with its nutritional content, fiber, flavonoids, and BAL >10⁸ CFU/g, which has the potential to serve as a functional food and probiotic source to help improve digestive health in the elderly. Further testing is recommended directly on elderly groups, along with product shelf-life testing.

Tassa Tassa; Ardi Mustakim

Pentagon : Jurnal Matematika dan Ilmu Pengetahuan Alam 2025 Asosiasi Riset Ilmu Matematika dan Sains Indonesia

Bekasam is a traditional fermented food made from snakehead fish (Channa striata) through spontaneous fermentation with the addition of salt and rice over several days. This fermentation process promotes the growth of indigenous microorganisms, particularly lactic acid bacteria (LAB), which play a crucial role in developing distinctive flavors and potentially providing health benefits. LAB are well-known for their probiotic effects, such as maintaining gut microbiota balance and enhancing immune system function. This study aimed to identify the presence of LAB in bekasam using the Gram staining method. Samples were taken from bekasam that had been fermented for three days. Bacterial isolation was conducted by culturing the samples on appropriate media, followed by Gram staining to observe bacterial morphology and cell wall characteristics under a microscope. Observations revealed a predominance of Gram-positive, rod-shaped (bacillus) bacteria, which are the primary morphological characteristics of LAB. This morphology is consistent with bacterial groups such as Lactobacillus spp., which are commonly found in traditional fermented products. These findings strengthen the assumption that snakehead fish–based bekasam is not only a traditional food source with rich flavors but also has potential as a natural probiotic source. This potential opens opportunities for bekasam to be developed as a functional food that supports digestive health and immune function. Further research is recommended to perform molecular identification to accurately determine bacterial species and to evaluate their probiotic activity in vivo. Thus, bekasam holds not only cultural and culinary value but also added value in the field of health and the functional food industry.

Wulandari Rihhadatul Aisy; Nurul Hayati; Muhajir Muhajir; Angga Pratama Putra

Manfish: Jurnal Ilmiah Perikanan dan Peternakan 2025 Asosiasi Riset Ilmu Tanaman Dan Hewani Indonesia

This study aims to determine the effect of adding EM4 probiotics in various doses to commercial feed on the growth of jatimbulan tilapia (Oreochromis niloticus L). Probiotics are known to play a role in increasing digestive efficiency, nutrient absorption, and maintaining the health of the fish's digestive tract, so they are expected to support optimal growth. The research method used was a Completely Randomized Design (CRD) with four treatments, namely: P0 (control/without probiotics), P1 (5 ml EM4/kg feed), P2 (7 ml EM4/kg feed), and P3 (11 ml EM4/kg feed), each with four replications. The parameters observed were the growth in length and weight of jatimbulan tilapia during the maintenance period. The results showed that the administration of EM4 probiotics at certain doses was able to provide a significant effect on the growth of tilapia compared to the control treatment. Treatment P2 (7 ml/kg feed) provided the best growth results compared to other doses, both in terms of increasing length and body weight. Meanwhile, administering probiotic doses that are too low (P1) or too high (P3) tends to be less effective in supporting optimal growth. This is thought to be due to the disruption of the microbial balance in the digestive tract when the probiotic dosage is incorrect. This research provides useful information for Jatimbulan tilapia cultivation practices, particularly regarding the use of probiotics to increase growth efficiency. Using the correct probiotic dosage can be a strategy to increase the productivity and sustainability of freshwater fish farming.

Nawra Nurvidilla; Ardi Mustakim

Jurnal Pendidikan Kimia, Fisika dan Biologi 2025 Asosiasi Riset Ilmu Pendidikan Indonesia

Cell-based therapy is an innovative approach currently experiencing rapid development in the biomedical field, particularly in the areas of immunotherapy and gene therapy. This approach utilizes living cells, such as genetically modified T cells, to recognize and destroy abnormal cells such as cancer cells or cells with genetic mutations. The development of gene engineering technologies, such as CRISPR/Cas9, has enabled scientists to precisely edit damaged genes, thereby repairing or restoring normal cell function. This study aims to examine recent advances in cell-based therapy, focusing on cell modification techniques, the efficiency of the immune system in targeting cancer cells, and the integration of gene therapy technology and immune cells. Furthermore, this study also identifies several challenges that remain, including the risk of side effects due to undesirable immune reactions, the genetic stability of the modified cells, and the long-term sustainability or viability of transplanted cells. While these challenges remain serious concerns, several clinical studies have shown that cell-based therapy has significant potential in the treatment of difficult-to-treat diseases, such as advanced cancer and rare genetic disorders. The results of this study demonstrate that cell-based therapy not only offers a new approach to treatment but also opens the door to more personalized and precise therapies. However, for broader implementation in clinical practice, further, in-depth research is needed regarding safety, long-term efficacy, and strict regulation. This research is expected to make a significant contribution to the development of more effective and safe future therapies.

Hendri Purwanto; Zulkarnain Zulkarnain

Zoologi: Jurnal Ilmu Peternakan, Ilmu Perikanan, Ilmu Kedokteran Hewan 2025 Asosiasi Riset Ilmu Tanaman dan Hewan Indonesia

Probiotics from West Sumatra curd have the potential to improve the production performance of local West Sumatra ducks, where Probiotics are live bacteria given as food sulpementation. Giving probiotics can have a beneficial effect on health where probiotics produce inhibitor compounds so that they can maximize the absorption of food in the intestines of livestock. The purpose of this study was to observe the production performance of ducks given probiotics. The design used was a randomized complete factorial method. The first factor (A) Dosage of probiotics (LAB) is with treatment A1: 1 ml (12.7 x 108 CFU/g); A2: 2 ml (25.4 x 108 CFU/g); A3: 3 ml (38.1 x 108 CFU/g). The second factor (B) is the frequency of probiotic administration, namely B1: 0 times; B2: 1 time; B3: 2 times; B4: 3 times; B5: 4 times; B6: 5 times; B7: 6 times, consisting of 4 replicates with a total of 346 ducks. The observed variables were food consumption, body weight gain, ration conversion, carcass percentage. The results of this study were the average food consumption at a dose of 3 ml with 5 times administration (995.79g). The highest average body weight gain (369.74 grams) dose of 1 ml and 5 times administration. The highest carcass percentage with a dose of 3 ml and a frequency of 5 times (72.63%).

Gigih Sugianto; Maria Agustini; Indra Wirawan

Zebra: Jurnal Ilmu Peternakan dan Ilmu Hewani 2025 Asosiasi Riset Ilmu Tanaman Dan Hewani Indonesia

One of the problems of catfish cultivation (Clarias gariepinus) that can hinder this cultivation business is the slow growth of fish weight. The purpose of this study is to determine the effect of different administration of Mina Pro probiotics on the absolute weight growth of pearl catfish seeds measuring 5-7 cm in the maintenance tub. The method used in this study is an experimental method. The design used in this study was a Complete Randomized Design (RAL) with four treatments and six replicates. The treatment given is: addition of probiotics mina pro at a dose of 2 ml/kg of feed, 4 ml/kg of feed, 6 ml/kg of feed and without the addition of probiotics to feed. Other parameters that were also studied included water quality including temperature, pH and DO.

Antoni Tri Mahendra; Didik Budiyanto; Sri Oetami Madyowati

Mikroba : Jurnal Ilmu Tanaman, Sains Dan Teknologi Pertanian 2025 Asosiasi Riset Ilmu Tanaman Dan Hewani Indonesia

The use of probiotics is very important in cultivation activities. Probiotics are live microorganisms that have a beneficial effect on the host by balancing the microflora in their intestines. In increasing the nutritional content of artificial feed (pellets), the bacteria in probiotics have a mechanism for forming several enzymes for digesting feed. This study aims to determine the effect of giving delacto probiotics at different doses in artificial feed on the absolute weight growth of tilapia (Oreochromis niloticus). Probiotics are mixed with feed using a biofloc cultivation system. The method used in this research was an experimental method with a Completely Randomized Design (CRD) with 4 treatments and 6 replications, namely by adding different doses to the treatments. Treatments A (8ml/kg), B (16 ml/kg), C (24 ml/kg) and D (32 ml/kg). The parameter observed was absolute weight growth. Water quality supporting parameters include temperature, pH and DO. Data were analyzed using the ANOVA test. Based on the research results, it shows that giving different doses of delacto probiotics has a significant effect on the absolute weight growth of tilapia (Oreochromis niloticus). Treatment A (8 ml/kg) gave the lowest absolute weight growth of 32.83 grams and treatment D (32 ml/kg) gave the highest absolute weight growth of 40.57 grams. Water quality parameters include water temperature ranging from 25.3 – 26.1֯C, acidity ranging from 7.5 – 8.0. And dissolved oxygen ranges from 4.7 – 5.3 ppm. The results of analysis of variance (ANOVA) for the three water quality parameters showed no differences between treatments (Oreochromis niloticus) fry.

Prima Farhan Septian; Didik Budiyanto; Sumaryam Sumaryam

Mikroba : Jurnal Ilmu Tanaman, Sains Dan Teknologi Pertanian 2025 Asosiasi Riset Ilmu Tanaman Dan Hewani Indonesia

Vannamei shrimp is one of the shrimp species with economic value and is one of the national leading commodities. On the other hand, one of the problems in vannamei shrimp cultivation is its slow growth, slow growth is caused by the less optimal absorption of nutrients in the feed. In dealing with these problems, one of the things that is done is the administration of probiotics. The addition of probiotics such as Biolacto containing Lactobacillus sp bacteria to the feed can increase the appetite of shrimp. This study aims to determine the effect of biolacto probiotics on feed on the growth of the absolute weight of vannamei shrimp. This study used a Complete Random Design (RAL) with four treatments: A (feed without a mixture of probiotics), B (probiotics 2 gr/100 gr feed), C (probiotics 4 gr/100 gr feed), and D (probiotics 6 gr/100 gr feed). Treatment B with a concentration of probiotics 2gr/100g feed gave the highest absolute weight growth of 9.2 gr. Water quality data during the study were obtained with water temperature ranging from 28.5 – 29.2°Celsius, salinity ranging from 36 – 37 ppt, pH ranging from 7.5 – 8.0, and dissolved oxygen ranging from 4.0 to 4.63 mg/l. The results of the ANOVA analysis of the four water quality parameters showed no difference between treatments and were in the optimal range of life of vannamei shrimp (Litopenaeus vannamei).

Made Saha Apta Raditya; Didik Budiyanto; Sumaryam Sumaryam

Mikroba : Jurnal Ilmu Tanaman, Sains Dan Teknologi Pertanian 2025 Asosiasi Riset Ilmu Tanaman Dan Hewani Indonesia

Giving probiotics such as probiotic boster on feed greatly helps the growth of absolute weight in Catfish seeds because the good bacteria contained in these probiotics help facilitate catfish seeds in digesting the feed so as to increase the efficiency of the feed. This study aims to see how effective the probiotic administration is. The study was conducted in Kramas District, Gianyar regency, Bali Province for 30 days using experimental methods, using a complete randomized design with a total of 4 treatments and 6 repetitions. Treatment A is by giving 3 ml of probiotic boster every 1 kg of feed. Treatment B by giving 5 ml probiotic boster every 1 kg of feed. Treatment C by giving 7 ml probiotic boster every 1 kg of feed. Treatment D by giving 9 ml probiotic boster every 1 kg of feed. In this study showed significantly different results on the addition of probiotic boster with different doses on the growth of absolute weight of catfish fry aged 1 month-2 months. Treatment D gave the highest result with an absolute weight gain of 55.33 grams, and treatment a showed the lowest result with an absolute weight gain of 25.17 grams. Water quality Data during the study obtained water temperature ranges from 27-29 oC, acidity ranges from 7-8, and dissolved oxygen ranges from 3.34 to 4.26 ppm. The results of the analysis of variance (ANOVA) of the three parameters of water quality there is no difference between the treatment and the life range of catfish fry (Clarias sp.)

Oktovandiansyach Tegar Imanan; Didik Budiyanto; Sumaryam Sumaryam

Manfish: Jurnal Ilmiah Perikanan dan Peternakan 2025 Asosiasi Riset Ilmu Tanaman Dan Hewani Indonesia

The addition of probiotics such as Lactobacillus sp to fermented feed is expected to improve nutrient digestibility, suppress the growth of pathogenic microorganisms, and improve feed conversion efficiency, thereby promoting optimal shrimp growth. This study used an experimental method on vannamei shrimp doc 80 using a complete randomized design (RAL) with four treatments: P0 (feed without fermentation as a control), P1 (feed fermentation for 0 hours 10ml/100g with Lactobacillus sp), P2 (feed fermentation for 24 hours with 10ml/100g Lactobacillus sp), P3 (feed fermentation for 48 hours 10ml/100g Lactobacillus sp), P3 (feed fermentation for 72 hours 10ml/100g Lactobacillus sp) by doing 6 repetitions which were carried out for 1 month/30 days in the maintenance tub. The results showed that Treatment A without fermentation gave absolute growth with an average of 1 gr, Treatment B with no fermentation gave absolute growth with an average of 1.89 grams, Treatment C with a fermentation time of 48 hours gave absolute weight growth with an average of 2.15 grams, Treatment D with no fermentation gave absolute growth with an average of 0.74 grams. Meanwhile, the water quality parameters during the study were obtained with water temperature ranging from 28.3-29.6o C, pH ranging from 7.8-8.0, and dissolved oxygen ranging from 4.03-4.66 mg/l. With the anova test, the absolute weight growth showed that with different fermentation time differences, there was a real effect on the growth of vannamei shrimp in C treatment, which was 2.15 gr. And in the results of the anova test, the four water quality parameters were not different between the treatments and were in the optimal range in the life of vannamei shrimp. Based on this study, it can be suggested that the optimal fermented feed is in the time range of 48 hours and there is further research for doc 80 and above.

Justian Trisna Nugraha; Budi Utomo; Koosdaryani Soeryodarundio

Konstruksi: Publikasi Ilmu Teknik, Perencanaan Tata Ruang dan Teknik Sipil 2024 Asosiasi Riset Ilmu Teknik Indonesia

This study aims to analyze the impact of administering commercial probiotic bacteria on the wastewater from tofu industries on changes in Bio-logical Oxygen Demand (BOD) and Dissolved Oxygen (DO) levels. Tofu industry wastewater is known to have high BOD and low DO levels, which pose a potential environmental pollution risk. This study used a pure experimental method with variables including the ratio of tofu industry wastewater to commercial probiotics (1:1, 1:2, 1:3) with an incubation interval of 8 hours. The results showed that the administration of commercial probiotic bacteria significantly reduced BOD levels and increased DO levels in the wastewater. The 1:3 ratio of tofu industry wastewater to commercial probiotics showed the most optimal effect in increasing DO and decreasing BOD. An incubation time of 32 hours showed the most optimal effect in increasing DO and decreasing BOD. The 1:3 volume ratio between tofu wastewater and probiotics (25% tofu wastewater and 75% probiotics) proved to be the most effective, with the highest DO increase of 2.51 mg/L and the largest BOD decrease of 144.20 mg/L. In addition, incubation time also had a significant effect, the longer the incubation time, the more optimal the probiotic activity in reducing BOD levels and increasing DO levels. The conclusion of this study is that the administration of commercial probiotic bacteria at a 1:3 ratio and an incubation time of 32 hours is an effective way to improve the quality of tofu industry wastewater.

Budi Utomo; Solichin Solichin; Fazrul Fauzan Munadi

Konstruksi: Publikasi Ilmu Teknik, Perencanaan Tata Ruang dan Teknik Sipil 2024 Asosiasi Riset Ilmu Teknik Indonesia

Indonesia boasts a number of tofu processing facilities totaling, around 84,000 both scale and large scale operations. These facilities collectively consume 2.56 million tons of soybeans each year. The high volume of tofu production poses a threat to the environment due to the generation of waste. If this waste is directly released into water bodies it can alter the waters characteristics potentially harming life. To address this issue Tofu Industry Wastewater can be implemented by introducing probiotics containing microorganisms that aid in breaking down matter and other components in the waste. This study focuses on analyzing bacterial colonies in Tofu Industry Wastewater by examining bacterial colonies present in these products. Various mixtures of Tofu Industry Wastewater and commercial probiotics will be tested at ratios of 1:1, 1:2, 1:3, and 1:4 over time periods ranging from 24, 48, 72, 96, 120, and 144 hours. The findings indicate that incorporating probiotics into Tofu Industry Wastewater can lead to a reduction in pollution levels.

Kristian Harris; Budi Utomo; Koesdaryani Soeryodarundio

Konstruksi: Publikasi Ilmu Teknik, Perencanaan Tata Ruang dan Teknik Sipil 2024 Asosiasi Riset Ilmu Teknik Indonesia

The tofu industry in Indonesia generally utilizes simple processing technologies, leading to unresolved wastewater management issues. Tofu wastewater contains high organic matter, measured by BOD, a parameter indicating the oxygen required by microorganisms to decompose organic matter. High BOD can decrease dissolved oxygen (DO) levels in water, which is crucial for aquatic life. This study investigates the effectiveness of local probiotics in reducing BOD and increasing DO in tofu wastewater (ABIT). Three treatments were applied: pure ABIT, neutralized ABIT (ABIT-N), and ABIT mixed with local probiotics in three ratios (1:1, 1:2, and 1:3). DO was tested at 0, 8, 16, 24, and 36 hours, while BOD was tested at 0 and 5 days. The results demonstrate that local probiotics can effectively reduce BOD and enhance DO levels in ABIT. The highest DO (3.36 mg/L) was observed in ABIT-N mixed with local probiotics at a 1:3 ratio after 32 hours. The most significant BOD reduction (135.77 mg/L) was also achieved at the 1:3 ratio. These findings suggest that local probiotics offer an eco-friendly approach to treating tofu wastewater.  

Harviel Adzan Megantyaka; Budi Utomo; Solichin Solichin

Konstruksi: Publikasi Ilmu Teknik, Perencanaan Tata Ruang dan Teknik Sipil 2024 Asosiasi Riset Ilmu Teknik Indonesia

Environmental pollution has become a serious problem in the world, including in Indonesia. Tofu waste is waste from processing soybeans and consists of solid and liquid waste. Solid waste in the form of dirt left over from cleaning is usually called tofu dregs, while liquid waste is water resulting from washing tofu. Industry knows that disposing of waste directly into water bodies can pollute those waters, because it can lower the pH of the air and affect aquatic biota. Therefore, the industry knows that it must carry out processing first to prevent environmental problems. In this study, researchers used commercial probiotic bacteria to reduce BOD and COD levels in tofu wastewater. The research was carried out by testing in the laboratory and using statistical data with graphic methods which can provide a comparison of the water content of tofu waste before and after it was given. The action given in the research was a ratio of 1:1, 1:2 and 1:3 water to tofu waste with commercial probiotic bacteria. Apart from that, researchers also added incubation times within each 8 hour interval, namely at 0 hours, 8 hours, 16 hours, 24 hours and 32 hours. The results of the research show that administering commercial probiotics can reduce BOD and COD levels in tofu wastewater. The most significant reduction occurred in a ratio of 1:3 in COD and BOD to 242.69 mg/lt and 148.00 mg/lt with an incubation time of 32 hours. This proves the commercial effect of probiotic bacteria to reduce BOD and COD levels so that it can help reduce environmental pollution, especially in the air.      

Retno Hartati; Widianingsih; Ria Azizah

Jurnal Suara Pengabdian 45 2024 LPPM Universitas 17 Agustus 1945 Semarang

Tujuan  pengabdian masyarakat adalah pengenalan metode dan teknologi tepat guna  dengan metode akuamber  untuk teknik memadukan  usaha bertanam sayur mayur  dengan budidaya ikan dalam wadah ember.  Pelaksanaan  kegiatan di lahan  sempit, pekarangan  dan masih terkena sinar matahari karena tanaman sayuran dan ikan membutuhkan sinar matahari.   Hasil usaha dapat dimanfaatkan konsumsi, peningkatan gizi bagi Balita dan anak  serta anggota keluarga yang lain. Bahkan dapat dikonsumsi bersama dalam kegiatan rukun tetangga (RT/RW) dan kegiatan PKK sebagai usaha swadaya masyarakat.  Keberlanjutan kegiatan  PPM kepada  kelompok sasaran  dapat meningkatkan ketahanan pangan keluarga dengan menerapkan aplikasi probiotik pada budidaya polikultur antara ikan dan tanaman sayuran skala rumah tangga.

Komalasari, Husnita; Karni, Ine; Heldiyanti, Rina; Arianto, Ahmad Rudi; Rahayu, Endang Sutriswati

JITIPARI (Jurnal Ilmiah Teknologi dan Industri Pangan UNISRI) 2024 Universitas Slamet Riyadi Surakarta

Yoghurt drink is a product obtained from fermentation of milk by certain lactic acid bacteria. The aim of this research was to determine how the inoculation time of the indigenous probiotic bacteria Lactobacillus plantarum Dad-13 influences the physicochemical and organoleptic properties of functional plain yoghurt probiotic drink products. This research used an experimental method with a completely randomized design with a single factor, 4 treatments and 3 replications. Reaserch data were analyzed using diversity analysis and Duncan's multiple distance test at a significance level of 5% using SPSS and Minitab 17 software. The results showed that the use of probiotic bacteria significantly affected on viscosity, color L*, color a*, color b*,  °HUE, pH, total dissolved solid, and fat content, but did not significantly influenced on protein content. The use of probiotics as a starter in yoghurt drinks increased viscosity, color brightness, pH and fat content, and have a lower total dissolved solid. The best treatment in this study was joint-inoculation who had viscosity 290,67 mPa.s; color L* 89,46; color a* 2,92; color b* 1,61; °HUE 29,47; pH 5,00; total dissolved solid 16,67 °brix; fat content 2,34 %; and protein content 4,12%. For the scoring test, this treatment has a non-sour taste; the aroma is slightly sour; Slightly thick texture and homogeneous consistency. Meanwhile, the hedonic test has a neutral taste, aroma and texture as well as a color, appearance and consistency were quite liked by the panelists.

Grace Angela Philips; Dety Mulyanti

Jurnal Inovasi Riset Ilmu Kesehatan 2023 Pusat Riset dan Inovasi Nasional

Stunting adalah kondisi di mana anak di bawah 5 tahun memiliki gangguan pertumbuhan dan perkembangan akibat kekurangan gizi kronis dan infeksi berulang. Prevalensi stunting pada balita tahun 2022 adalah 21,6%. Angka ini masih diatas standar yang ditetapkan oleh WHO yakni 20%. Tujuan artikel ini adalah mengkaji artikel yang berhubungan dengan peram mikrobiota saluran cerna terhadap kejadian stunting di Indonesia.  Sumber data didapatkan melalui database Google Scholar dan ScienceDirect dengan kriteria artikel penelitian yang relevan tentang mikrobiota saluran cerna terhadap stunting dan dilakukan di Indonesia, desain penelitian longitudinal, kasus kontrol, maupun cross-sectional, publish tahun 2018-2023, berbahasa Indonesia atau Inggris dan bersifat open access. Didapatkan 5 artikel yang sesuai kriteria inklusi kemudian dilakukan sintesis data. Hasil penelitian menunjukkan bahwa mikrobiota non patogen yang mendominasi di saluran cerna pada balita dapat membantu terjadinya stunting pada anak. Hal ini disebabkan peran mikrobiota tersebut dalam penyerapan zat gizi serta memperkuat sistem imunitas tubuh anak.

Afrida, Milu; Purwati Nurlaili, Enny; Hermanu, Bambang

Jurnal Agrifoodtech 2023 Universitas 17 Agustus 1945 Semarang

Soyghurt adalah minuman probiotik yang terbuat dari sari kedelai dengan penambahan bakteri asam laktat. Pembuatan Black Soyghurt yang berbasis sari kedelai hitam dengan penambahan ekstrak bunga telang dan starter yoghurt. Tujuan penelitian ini untuk mengkaji pengaruh lama fermentasi dan penambahan ekstrak bunga telang terhadap sifat fisik black soyghurt. Metode penelitian menggunakan faktorial (2x2) dua faktor dengan dasar RAL (Rancangan Acak Lengkap) dan dibandingkan dengan produk komersial sebanyak 3 kali ulangan, sehingga didapatkan 15 unit percobaan.  Faktor pertama lama fermentasi 10 jam dan 15 jam. Faktor kedua penambahan ekstrak bunga telang 8% dan 13%. Hasil analisis statistik apabila menunjukkan berbeda nyata maka akan dilakukan uji lanjut Duncan’s Multiple Range Test (DMRT). Hasil penelitian pada sifat fisik black soyghurt nilai viskositas 75,00-475,00 mPas,  pada uji warna L* 50,81-53,19, a* 10,87-16,06, b* (-1,60)-3,39. Simpulan penelitian ini adalah pengaruh lama  fermentasi dan penambahan ekstrak bunga telang tidak berpengaruh terhadap sifat fisik. warna L * kecerahan (putih-hitam) dan warna b* (biru-kuning), tetapi berpengaruh terhadap viskositas warna a* (hijau-kemerahan).

Agung Guntara, Fahrizal; Yulianto, Wisnu Adi; Pujimulyani, Dwiyati; Wulandari, Widya; Kasuci, Okavia

JITIPARI (Jurnal Ilmiah Teknologi dan Industri Pangan UNISRI) 2022 Universitas Slamet Riyadi Surakarta

Tape is a traditional fermented food whose benefits can be increased by adding probiotic cells such as Saccharomyces boulardii and Lactobacillus plantarum Dad-13. Tape tends to be easily damaged, so to maintain the viability of probiotics and as a food diversification, tape can be substituted for ice cream dough. The purpose of this research was to determine the effect of substitution and the proportion of substitution of glutinous rice and cassava tape in the manufacture of ice cream on the characteristics and preference level of probiotic ice cream. This research method was carried out using a completely randomized design with a factorial pattern with two factors, the first factor was the type of tape, and the second factor was the proportion of substitution. The type of tape used are white cassava tape, yellow cassava tape, white glutinous rice tape, and black glutinous rice tape with substitution proportions of 0%, 10%, 20%, 30%, and 40%. Analysis of probiotic sticky rice tape and cassava ice cream included water content, total solids, melting time, antioxidant activity, total lactic acid bacteria, total yeast, and preference level. The data obtained will be calculated using the Anova statistikal method. If there is a significant difference between treatments, it is carried out with Duncan's Multiple Range Test significant difference test at a confidence level of α= 5%. The results showed that glutinous rice and cassava tape types and their substitution proportions affected the water content, total solids, melting time, antioxidant activity, total lactic acid bacteria, total yeast, and preference level. Based on the preference level and antioxidant activity, the best ice cream was produced with the substitution of 20% white cassava tape or 20% yellow cassava tape, which had an antioxidant activity of 32.41% and 33.17% RSA and total lactic acid bacteria of 1.79 x 108 and 1.67 x 108 CFU/g, respectively.

Gunawan Wijonarko, Luluk Arum Mawar Nur Aini

JITIPARI (Jurnal Ilmiah Teknologi dan Industri Pangan UNISRI) 2018 Universitas Slamet Riyadi Surakarta

Sari ubi jalar yang mengandung senyawa antioksidan dan ditambahkan kultur bakteri probiotikmenghasilkan produk pangan fungsional untuk menjaga kesehatan. Penelitian ini bertujuan, yaitu: 1)Mengetahui komposisi optimum formula minuman sinbiotik yang dihasilkan; 2) Mengetahui pengaruhsubstitusi rasio susu skim dan sari ubi jalar (1:3, 1:1 dan 3:1) dan penambahan konsentrasi (2%, 3% dan4%) terhadap sifat fisik, mikrobiologi, kimia dan sensori minuman sinbiotik yang dihasilkan sehinggamenghasilkan produk minuman sinbiotik terpilih. Metode yang digunakan yaitu Response SurfaceMethodology (RSM) pada perangkat lunak Design Expert v.9.06. Minuman sinbiotik dengan formula terbaikyaitu persentase Lactobacillus casei 2% dan perbandingan sari ubi jalar : susu skim (3:1) Memiliki hasilnilai pH 5,6, total padatan terlarut 26o Brix, total probiotik 1,56x 109 CFU, ketahanan terhadap pH rendah337,4 %. Viabilitas minuman sinbiotik dengan formula terbaik menunjukkan penurunan dari 1,56x 109 CFUmenjadi 6,3,2x108CFU pada minggu ke tiga. Jumlah total BAL pada penelitian ini sudah memenuhi syaratsebagai minuman fungsional pada SNI yogurt yaitu SNI (2981:2009) sebesar 1x107 CFU dan masih dapatdikonsumsi. Karakteristik sensoris berwarna coklat, viskositas sedikit kental, rasa enak dan tingkat kesukaan(overall) disukai.Kata kunci : Formulasi, Lactobacillus casei, minuman sinbiotik, ubi jalar