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Nosocomial infections are a serious problem that often occurs in hospitals and can have a negative impact on patient safety. The role of nurses, especially in inpatient rooms, is very important in preventing these infections. Preliminary studies found 0.34% of HAIs in hospitals, 0.00% of urinary tract infections, 0.86% of Phlebitis, 0.00% of Ventilator Associated Pneumonia. The aim of this study was to determine the relationship between knowledge and behavior of nurses regarding the prevention of nosocomial infections.This type of research is quantitative research with a cross sectional approach. Data collection was carried out through questionnaires. This population is all nurses in the Inpatient Room at PKU Muhammadiyah Sukoharjo Hospital. The sample for this research was 42 respondents who were selected using the total data saturated sampling technique used, namely univariate and bivariate analysis (Spearman rho test).The results of the univariate analysis research showed that the nosocomial infection prevention process variable, nurse knowledge was in the good category at 100% and the nosocomial infection prevention process variable, nurse behavior was in the good category at 100%. The results of bivariate analysis using the Spearman's rho statistical test show that there is a p-value of 0.000, where this value is smaller than 0.05 with a coefficient value of 0.528. So it can be said that there is a relationship between knowledge and behavior of inpatient room nurses in preventing nosocomial infections at the PKU Muhammadiyah Sukoharjo hospital.In conclusion, there is a relationship between knowledge and behavior in preventing nosocomial infections at the PKU Muhammadiyah hospital. Suggestions: Increase counseling and training on preventing nosocomial infections for nurses and health workers and non-health workers at the PKU Muhammadiyah Sukoharjo Hospital.

Background:Hand hygiene is an effective step to break the chain of infection transmission, so that nosocomial incidence can be reduced. Infection prevention and control absolutely must be carried out by nurses, doctors and all people involved in patient care. Nurses' hand hygiene behavior is one of the factors that has a big influence on preventing nosocomial infections in hospitals. At Hospital X in 2014 the proportion of phlebitis incidents was 6.5%. Phlebitis is a nosocomial infection. The results of observations of four nurses who carried out medical procedures, one of them did not wash his hands before and after carrying out procedures on patients, while the other three had already washed their hands. Research Objective: To determine the relationship between nurses' knowledge and attitudes about infections nosocomial with hand hygiene behavior at Hospital Data analysis used the Chi-square test with a confidence level of 95% (α= 0.05). Research Results: Characteristics of nurses at Hospital 56.7%). Most of them were women, namely 25 nurses (83.3%). The most work period is 1-5 years (56.7%). The highest level of education was D3 nursing (93.3%). Good knowledge about nosocomial infections was 26 nurses (86.7%). Most of the attitudes were positive, namely 27 (90.0%) nurses.  Most of them had good hand hygiene behavior19 (63.3%). There is a relationship between nurses' knowledge about nosocomial infections and hand hygiene behavior with a p value of 0.012 (p<0.05). There is a relationship between nurses' attitudes and hand hygiene behavior p of 0.041 (p<0.05). Conclusion: There is a relationship between nurses' knowledge about nosocomial infections and hand hygiene behavior and there is a relationship between nurses' attitudes and hand hygiene behavior.