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Eukaryotic microorganisms, particularly fungi and protists, play a vital role in ecosystems, as well as in microbiology studies and biology education. The complex structure of eukaryotic cells, such as a true nucleus and membrane-bound organelles, enables these two groups to perform diverse physiological functions and adapt to their environments. This study aims to synthesize the literature on the structure and function of eukaryotic cells in fungi and protists and their implications for biology learning. The method used is a literature review with a descriptive qualitative approach through analysis of relevant scientific sources. The results of this study indicate that understanding the structure of eukaryotic cells in fungi and protists is not only important in the context of microbiology but also contributes to strengthening understanding of the concept of cell structure and function and making biology learning more contextual and meaningful.

Cassava (Manihot esculenta) is a staple food that is widely consumed by the Indonesian population, especially in rural areas. However, during post-harvest handling and storage, cassava is highly susceptible to contamination by microorganisms, particularly fungi. These fungi can cause damage to the food, reduce its nutritional quality, and even produce mycotoxins that are harmful to human health. The increased risk of contamination has become an important concern in efforts to improve the quality and safety of cassava food products. This study aimed to identify the morphology of fungal colonies and the characteristics of fungal cell walls isolated from cassava stored in open environmental conditions for several days. The methods used in this study included isolation using Potato Dextrose Agar (PDA) media, macroscopic observation of fungal colony morphology, including color, edge, elevation, and texture, as well as Gram staining to observe the fungal cell wall properties. The results of this study revealed a diversity of fungal colony morphology, with three dominant types suspected to belong to the genera Aspergillus, Penicillium, and Rhizopus. Gram staining showed that the three isolates were characterized as Gram-negative, indicated by the absorption of safranin as the secondary stain. This study provides an initial overview of the types of fungi that may develop on cassava during storage, and it offers a foundation for further studies on the toxicity and food microbiology applications related to cassava. Morphological identification and Gram staining play an essential role in the initial screening before molecular identification is performed.

This study aims to explore the potential of cassava extract (Manihot esculenta)-based growth media for isolating microorganisms from soil. The use of alternative culture media is crucial, especially in regions with limited access to commercial media such as Nutrient Agar or Potato Dextrose Agar, which are relatively expensive. Cassava, a tropical tuber rich in starch and widely available in Indonesia, offers significant promise as a natural substrate for microbial growth. Microbial isolation was performed using a serial dilution method on soil samples collected from an organic garden. The media was prepared from cassava juice mixed with agar and glucose, sterilized, and poured into petri dishes. After inoculation, microbes were incubated for seven days at room temperature. Colony morphology was observed macroscopically, and Gram staining was used to identify bacterial characteristics. The results revealed that cassava extract media effectively supported the growth of diverse microbial colonies, varying in shape, color, and edge structure. Most isolates were identified as Gram-positive, with rod and coccus forms. The presence of pigmented colonies indicates the potential of certain isolates to produce bioactive compounds. In conclusion, cassava extract-based media is a viable alternative for soil microbiology studies. It is not only cost-effective but also facilitates the exploration of local microbial diversity with potential applications in biotechnology, including bioremediation and enzyme production. This approach encourages the use of indigenous resources in scientific research, promoting sustainability and accessibility in microbiological practices.

Solid waste from the tapioca processing industry, which is based on cassava (Manihot esculenta), is a medium rich in organic matter, particularly starch, that supports the growth of various types of microorganisms, especially fungi and bacteria. This study aims to isolate and observe the morphology of microorganisms from solid waste of cassava from the tapioca industry using Potato Dextrose Agar (PDA) medium in a series of pharmaceutical microbiology practicum. Waste samples were taken from the tapioca processing site, then underwent a serial dilution process and inoculation into PDA medium, which was incubated at 28–30°C for 5 days. The isolation results showed the growth of microorganisms with diverse colony morphologies, which are suspected to originate from the genera Aspergillus, Penicillium, and Rhizopus, based on macroscopic and microscopic characteristics. The presence of these microorganisms indicates the potential utilization of cassava waste as a source of microbial isolates for biotechnological and pharmaceutical applications, such as enzyme production and development of bioactive compounds. This practicum activity also contributes to increasing students' understanding of isolation techniques, microorganism characterization, and their role in biological-based waste management.

Bulian coconut chips are a typical Jambi food made from dried and fried old coconuts. The traditional production method and inadequate hygiene standards make this product susceptible to microbial contamination, especially harmful bacteria. This study aims to identify the types of bacteria that may be present in Bulian coconut chips using culture and Gram staining methods as the initial step for morphological identification. Samples were taken from three local producers in Bulian District, Batanghari Regency, Jambi. Testing was carried out by serial dilutions up to 10⁻⁴, then planted on Nutrient Agar (NA) media and incubated for 24 hours at 37°C. The colonies that grew were then observed morphologically and Gram staining was performed using a modified method without alcohol. The results showed the presence of various colonies with cream, white, and pale yellow colors. Based on the results of Gram staining, bacilli and cocci were found, which were included in the Gram-positive and Gram-negative categories. The types of bacteria suspected to appear include Bacillus sp., Staphylococcus sp., and possibly Escherichia coli. These findings indicate a risk of microbial contamination that could endanger consumer health, especially if the production process is not improved. Strict hygiene and sanitation principles are strongly recommended in the processing of this traditional food to ensure the product remains safe for consumption. The importance of implementing hygienic standards in the production of Bulian coconut chips is further emphasized by these findings. In addition to improving food safety, implementing proper sanitation procedures can also extend the product's shelf life and increase consumer confidence in local traditional foods. Efforts such as training local producers, using clean equipment, and hygienic packaging can help minimize the risk of bacterial contamination.

This study aims to identify the presence of Staphylococcus aureus in fermented pedo fish (Decapterus spp.) products using the Gram staining technique. The Gram staining technique is a simple but effective method to distinguish Gram-positive and Gram-negative bacteria based on differences in their cell wall structure. Samples were taken from traditional fermented pedo fish and analyzed in a microbiology laboratory. The results showed the presence of Gram-positive coccus-shaped bacteria that clustered like grapes, in accordance with the morphological characteristics of S. aureus. This identification can be used as an initial step in evaluating the microbiological quality of fermented products.

Crop productivity is one of the key components in supporting the sustainability of the agricultural sector and global food security. However, challenges such as soil degradation, dependence on chemical fertilizers, and climate change necessitate more innovative and environmentally friendly approaches. Soil microorganisms play a significant role in improving crop productivity through biological mechanisms such as nitrogen fixation, organic matter decomposition, mycorrhizal symbiosis, and the production of bioactive compounds that protect plants from pathogens and abiotic stress. Microbiology-based biotechnology approaches, such as the use of biofertilizers and biopesticides, offer environmentally friendly solutions to address modern agricultural problems. This research aims to review the role of microorganisms in improving crop productivity, focusing on the application of microbiology-based biotechnology. The research method used is a literature study or meta-analysis of relevant literature and research in the last 10 years. The results of the discussion show that microorganisms such as Rhizobium, Azospirillum, Trichoderma, and mycorrhiza have a major contribution in improving soil fertility, crop yields, and plant resistance to environmental stress.

Agricultural microbiology plays an important role in supporting the sustainability of the agricultural sector, contributing to global food security. Microorganisms, such as bacteria, fungi and algae, have various roles in increasing soil fertility, controlling plant pathogens and supporting biodiversity. This research aims to explore the role of microbiology in supporting environmentally friendly and sustainable agriculture. Through observations of various applications of microorganisms in agriculture, it was found that microorganisms can function as biopesticides, biofertilizers and organic waste processors. The research results show that microorganisms are able to optimize agricultural yields, improve soil health, and reduce dependence on synthetic chemicals.

Agricultural waste treatment is an organism that offers innovative solutions to reduce the negative environmental impact of waste while increasing agricultural productivity. By using microorganisms such as bacteria, fungi, and actinomycetes, wastes such as straw, manure, and coffee grounds can be effectively treated. This biological process accelerates the decomposition of organic matter into more benign substances and nutrients, such as nitrogen, phosphorus and potassium, which are essential for plant growth. In addition to improving soil fertility, the use of organic fertilizers also reduces dependence on chemical fertilizers, greenhouse gas emissions, and sustainable agriculture. The aim of this study was to investigate the role of microbiology in agricultural waste transformation by examining its mechanisms, potentials, and challenges. The results of this study show that microorganism-based technologies contribute to more environmentally friendly practices and reinforce fundamental economic principles. Processing agricultural waste into organic fertilizer is a strategic step in realizing sector sustainability.

Soil pollution is a global problem with anthropological and natural sources. Pesticides are chemical compounds used to eradicate pests. Pesticides are chemical or biological agents that can weaken, paralyze, and kill pests. According to a report by FAO (2021), global pesticide use reaches more than 4 million tons per year, with Asia accounting for nearly 50% of total consumption. In Indonesia, data from the Ministry of Agriculture (2022) shows that pesticide use increased by 15% in the last decade. Unfortunately, the intensive and uncontrolled use of pesticides has triggered the accumulation of harmful residues in the soil, creating a serious threat to soil, environmental, and human health. The purpose of this study was to determine how the utilization of microbiology in the bioremediation of agricultural soil polluted with pesticides. The method in this journal article uses a library study (library research), namely a method with data collection by understanding and studying theories from various literatures related to the research. The results show that soil bioremediation to reduce pesticides in agricultural soils can be done by utilizing specific or local microorganisms (bacteria and fungi), or enzymatic degradation. Although in laboratory scale, many findings on soil bioremediation are available in the literature, only few data on real scale activities can be found.    

This bacterial morphology observation training was conducted for grade X students at SMA Negeri 1 Parigi Tengah. The main problem faced was the lack of knowledge and skills of students in the field of microbiology, especially in identifying bacterial morphology. The purpose of this activity was to improve students' understanding of basic biological concepts and practical skills in bacterial observation. The methods used included delivering material on the principles and techniques of bacterial morphological characterization, as well as direct practice using microscopes and Gram staining techniques. The results showed that students successfully identified Staphylococcus aureus bacteria in the form of cocci with Gram positive and Bacillus sp. in the form of bacilli with Gram negative. This activity has proven effective in improving students' understanding and skills in microbiology, as well as encouraging their interest in studying biology further

This research and development aims to develop a valid and effective Microbiology module based guide inquiry on research on Isolation and Identification of Endophytic Bacteria from Ciplukan. The microbiology module was developed in reference to the ADDIE research and development model. The module drafting phase includes:1) the analyzing step through interviews with lecturers and the questionnaire of needs analysis by student respondents; 2) The step of designing learning modules to improve cognitive knowledge and process skills; 3) The step of developing by using module validation; 4) The step of applying which consists of module implementation in learning; 5) The evaluation step which consists of evaluating the validity of modules, cognitive learning outcomes and student science process skills. The results of the study show that the microbiology module developed accordance with the standards of validity, feasibility and practicality.