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Water plays a fundamental role in sustaining human life, particularly as a source of drinking water. Nonetheless, the consumption of water that does not comply with established health standards can result in illnesses such as diarrhea and vomiting. In the Patrang District, the use of refillable drinking water has gained popularity as an economical substitute for bottled water. Despite its convenience, there are growing concerns about the microbiological safety of refillable drinking water, especially regarding contamination by Coliform and Escherichia coli bacteria, which serve as key indicators of water hygiene and safety. The objective of this research was to determine the contamination levels of Coliform and Escherichia coli bacteria in refillable drinking water. The study utilized a quantitative research design with descriptive analysis and employed the Most Probable Number (MPN) method for testing. Data collection took place from May to June 2024, comprising five water samples labeled X, Y, Z, A, and B, obtained from refillable drinking water depots in Patrang District using a simple random sampling approach. The results indicated that out of the five samples tested, the sample labeled Z was positive for Coliform with an MPN value of 920 cfu/mL, while the sample labeled A was positive for both Coliform and Escherichia coli with an MPN value of 2,000 cfu/mL. From these findings, two samples (Z and A) were identified as contaminated, with the highest bacterial concentration found in sample A, which had an MPN value of 2,000 cfu/mL

. PT. XY is a company engaged in the printing sector, especially in the security products sector, which is required to process its wastewater so that it can meet the required wastewater quality standards, especially in the processing of domestic waste originating from human waste and other consumption waste such as washing water, rinsing and so on. A Sewage Treatment Plant is a domestic liquid waste processing installation to process residual domestic liquid waste so that it becomes clear and is no longer harmful to the environment. The problem that arises is one of the measurement parameters in the wastewater quality standard, namely total coliform (coliform bacteria), which has not met the requirements for several months. This study aims to process domestic waste in the sewage treatment plant process with the six-sigma method to achieve results according to the requirements of the liquid waste quality standard. It is safe to be discharged into water bodies. The results of this study from 6 parameters 5 parameters meet the wastewater quality standards, namely: pH, Total Suspended Solids / TSS, Biochemical Oxygen Demand5 / BOD5, Chemical Oxygen Demand / COD, Oil and fat, while the average level of Total Coliform in a year is 6,448 MPN / 100 ml, not yet meeting the wastewater quality standards of 3,000 MPN / 100 ml. The results of the six-sigma calculation found a result of 1.5 Sigma (σ) where the process is in the non-competitive category

Food is used as a basic necessity for human life. Food can increase the growth and development of the body and can repair damaged body tissue. Food and drinks that will be distributed and consumend by the public must comply with the quality standards  that have been set. One of the microorganisms most often found in food and drinks in Coliform bacteria. Coliforms are a group of bacteria that can be used as an indicator of the presence of pathogens that are detrimental or not beneficial for water, food, milk and dairy products. The aim of this research was to determine whether or not Coliform bacteria were present in white tofu sold at the Sedondong Batang market. The type of research used is descriptive research. This research was carried out on November 28, 2023. Sample examination was carried out at the Pekalongan Regency KESDA Laboratory. The samples used in this research were white tofu sold at the Sedondong Batang market, totaling 4 samples. In this research, the sampling technique was purposive sampling. The method used the MPN method, MPN is a method used to count Coliforms in water using in-tube fermentation testing. This method requires two tests, namely a predictor test and a reinforcement test.

Introduction: Water quality parameters are indicated by the presence of E. coli bacteria. These bacteria come from human feces, warm-blooded animals, and non-fecal coliforms. Decree of the Health Minister of the Republic of Indonesia Number 907/MENKES/VII/2002 concerning requirements and supervision of drinking water quality states that good drinking water has a zero total coliform and fecal coliform. Methods: This research uses a descriptive approach with a cross-sectional design. It aims to determine the quality of water sources in Sulkatan village. Ten (10) water sources were used as the samples using total sampling method. The collected samples were then taken to the laboratory to be tested with a series of MPN tests Results and Discussion: The results show that the amount of Escherichia coli bacteria in water samples in Sulkatan Village is 0MPN/100 ml. It is owing to the location of the water source that is far from industrial activities, household waste and residential areas. Conclusion: The quality of the tested well-water samples is good and within normal limits in accordance with the Decree of the Health Minister of the Republic of Indonesia Number 907/MENKES/VII/2002    

Domestic waste that is disposed of carelessly is one of the causes of soil pollution. This research was conducted in the village of Kuranji Nagari Lubuk Jantan, the measurement result are filled in based on meansuring the distance from the latrine to the dug well using a meter. Fecal Coliform variable, the MPN (Most Probable Number) test was used on dug well water with reference to SNI 06-4158-1996 at the Andalas University Water Laboratory. Of the 31 dug well facilities, there are 31 dug well facilities (100%) that have well water with Fecal Coliform that does not meet the requirements in accordance with the 2017. Research result that: 1. Based on the proportion of fecal coliform in Kuranji village, laboratory test result on 31 dug well samples do not meet the requirements for direct consumption in RI Minister of Health Regulation No. 416/Menkes/per/IX/1990 with a percentage of 100%. 2. The potential spread of fecal coliform contamination is due to inadequate contruction. 3. Of the 31 samples of dug wells and latrines that were observed, 23 dug wells had an unsatisfactory contamination distance of 11 meters with a contamination rate of 29 with a percentage of (93.55), 2 dug wells had a satisfactory distance to the latrine of 11 meters with a percentage of 6.50%.      

Ikan tongkol asap merupakan salah satu produk makanan olahan yang dijual di pasar induk Kabupaten Batang. Dalam proses menyajikan dagangan yang tidak higienis dapat menyebabkan ikan tongkol asap terkontaminasi bakteri. Bakteri Eschericia coli merupakan bakteri patogen yang dapat tumbuh akibat lingkungan yang tercemar.  Penelitian ini bertujuan untuk mengetahui adanya cemaran Eschericia coli pada ikan tongkol asap yang dijual di pasar induk Kabupaten Batang. Metode penelitian yang dilakukan adalah observasional deskriptif, dimana data diperoleh dari hasil analisa kuantiatif terhadap cemaran bakteri Eschericia coli dalam sampel berdasarkan Most Probable Number (MPN). Sampel penelitian berupa empat sampel ikan tongkol asap yang diperoleh dari keempat penjual berbeda. Dilakukan pengenceran pada 20 g/sampel ikan tongkol asap dengan aquadest steril dan dilakukan uji pendugaan menggunakan media Lactose Broth (LB), uji penegasan menggunakan media Brilliant Green bile Lactose Broth (BGLB). Hasil penelitian menunjukan sampel ITA 1, ITA 3, dan ITA 4 tidak memenuhi syarat kadar maksimum Eschericia coli dalam makanan yaitu < 3 /g sampel, sedangkan sampel ITA 2 memenuhi syarat kadar maksimum Eschericia coli dalam makanan yaitu < 3 /g sampel.

Drinking water produced by drinking water depots (DAM) is an alternative to meeting the drinking water needs of the community. Practicality and relatively cheaper prices when compared to bottled drinking water, become the main attraction for the community to consume drinking water produced by DAM. This study aims to determine the description of sanitation and bacteriological quality in refill drinking water depots in Kebun Cengkeh, Batu Merah Village, Sirimau Kecematan, Ambon City. The type of research used in this study is descriptive which aims to determine the content of E.coli bacteria and sanitation conditions at refill drinking water depots in Kebun Cengkeh, Batu Merah Village, Sirimau District, Ambon City. The population in this sample is the entire drinking water in all refill depots located in Kebun Cengkeh, Batu Merah Village, Sirimau District, Ambon City. The sample in this study was a portion of the refill drinking water depot. The results showed that 3 DAMIUs had a total score of <70 for sanitary inspection assessment and 3 DAMIUs contained E. coli >0 MPN/100 ml. 3 DAMIUs did not meet the sanitary inspection requirements and 3 DAMIUs did not meet the bacteriological requirements.

Ice cubes are a food product that is often consumed by the community. Ice cubes are usually mixed in drinks to add freshness. Ice cubes are made from drinking water frozen at 0°C, although low temperatures can inhibit the growth of microorganism cells, ice cubes still have the possibility of containing contaminant microorganisms. Ice cubes that can cause diarrhea are ice cubes that contain pathogenic bacteria, one of which is Escherichia coli. Therefore it is necessary to check the quality of ice cubes microbiologically. This study aims to detect the presence of Escherichia coli in ice cubes sold by food stalls in the Liliba Village, Kupang. This type of research uses a descriptive method, where the population in this study is ice cubes which are sold in 10 different stalls in Liliba Kupang Village. The method used is Most Probable Number (MPN) with a variance of 5-1-1 followed by Gram staining and IMVIC test. From the results of the study, it can be found that ice cubes are sold in food stalls in Liliba Kupang Village, from 10 samples of ice cubes there is 1 sample that contains 10% Escherichia coli bacteria.