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This study aims to investigate the effects of tamarind leaf extract (Tamarindus indica L.) on neuropharmacological activity in mice using calculated percentages of responses based on the parameters PSM, SSSP, DSSP, SL, RO, SM, PSL, and ANA. This research was conducted as a laboratory experiment using a completely randomized design (CRD) with three treatment concentrations: 1%, 2%, and 4%. Observations were performed to assess the percentage of activity produced by each sample concentration, followed by descriptive–quantitative analysis to determine the dose–response pattern. The results showed that tamarind leaf extract produced varying responses across concentrations. The SSSP, ANA, and RO effects demonstrated increased activity at the 2% concentration, whereas other parameters (PSM, PSL, SM, SL, and DSSP) showed decreased activity. Interestingly, the SSSP parameter exhibited a positive dose–response pattern with the highest activity of 55.84% at 2%. Overall, the effectiveness of tamarind leaf extract depends on the concentration level. The 2% concentration appears to be the optimal dose for several neuropharmacological effects, while the 4% concentration was most effective only for SSSP. These findings highlight the importance of multi-concentration testing to determine effective dosing of natural products for biological applications and the need for further investigation.

Persistent elevation of blood glucose levels defines diabetes mellitus, a chronic metabolic disorder that occurs when insulin production is insufficient, diminished sensitivity of insulin receptors, or a combination of both factors. The medicinal properties of red ginger (Zingiber officinale var. rubrum) are attributed to its rich content of bioactive compounds, including gingerol and shogaol, which are considered key contributors to its various therapeutic effects. The effectiveness of red ginger extract in lowering blood glucose levels in experimental mice was evaluated in this study. A true experimental design with a quantitative approach was applied, employing a pretest–posttest control group format. The study involved twelve samples, which were randomly allocated into four separate groups. The treatment phase was conducted over a period of seven days. Findings revealed mean values of 10.4 prior to the intervention and 15.2 afterward, indicating a notable increase. A significant difference between pre-treatment and post-treatment measurements in group K1 was revealed by the statistical analysis, which yielded a P-value of 0.001 (P < 0.05). It was observed that red ginger extract, given at 100 mg per kilogram of body weight, effectively reduced blood glucose levels in the mice studied..

Mimosine is an aromatic amino acid found in Mimosa pudica L. (sensitive plant) and is known to possess toxic properties that can damage various organs, including the kidneys. This study aimed to investigate the effect of ethanolic root extract of Mimosa pudica on renal damage in mice (Mus musculus). Twelve mice were divided into four groups: a control group and three treatment groups that received ethanolic root extract of M. pudica at doses of 10 mg/mouse/14 days, 100 mg/mouse/14 days, and 1000 mg/mouse/14 days. Following treatment, the mice were dissected to measure serum creatinine levels as an indicator of renal function, and histopathological observations of kidney tissues were performed using Hematoxylin-Eosin staining. The results revealed that administration of the ethanolic root extract of M. pudica significantly increased serum creatinine levels (P < 0.05), indicating impaired renal filtration function. Histologically, several types of tissue damage were observed, including glomerular atrophy, hydropic degeneration, and cellular necrosis, with hydropic degeneration being the most predominant and glomerular atrophy the least frequent. These findings suggest that mimosine, an active compound in M. pudica roots, exhibits a dose-dependent nephrotoxic effect, where increasing extract concentrations exacerbate renal tissue damage. Therefore, caution is advised in the medicinal use of M. pudica, as despite its pharmacological potential, high-dose or prolonged exposure may pose a serious risk of renal dysfunction.         

The root of Mimosa pudica L. contains mimosine, a toxic alkaloid, as well as tannins that exhibit toxic properties, potentially disrupting pancreatic function. Exposure to sufficiently high concentrations of toxic compounds can impair cellular metabolism, leading to irreversible damage. One of the cellular changes induced by cytotoxic compounds is the reduction in the size of pancreatic Langerhans islets. This study aimed to investigate the effects of ethanol extract of Mimosa pudica roots on the diameter of Langerhans islets and β-cell damage in the pancreas of mice. Twelve mice were randomly divided into four groups, each consisting of three animals. Group I served as a control and did not receive the extract, whereas Groups II, III, and IV were administered root extract at doses of 10 mg/g BW, 100 mg/g BW, and 1000 mg/g BW, respectively, for 14 consecutive days. The results demonstrated that administration of ethanol extract of Mimosa pudica roots reduced the diameter of pancreatic Langerhans islets in a dose-dependent manner, with higher doses causing more severe β-cell damage. These findings highlight the need for caution in the use of Mimosa pudica roots as traditional medicine or dietary supplements.

Tonic is a preparation thats can stqrengsthen the body and improve stamina. Curcuma zedoaria and Curcuma aeruginosa are known to have phytochemical compunds such as flavonoids, alkaloids, saponins, tannin, triterpenoids, curcuminoid and polyphenols that can function as stimulant and tonics. This studiy aimed to investigate tonic effects of ethanolic extracts of Curcuma zedoaria, ethanolic extract of Curcuma aeruginosa, and their combinations on male white mice (Mus musculus). Curcuma zedoaria rhizome and Curcuma aeruginosa rhizome were extracted using the maceration method for 3 days using 70% ethanol solvent which was then thickened in a waterbath. A total of 35 male mice were divided into 7 groups with different treatments, including negative control (aquadest), positive control (caffeine 100 mg/kgBB), ethanolic exctract of Curcuma zedoaria (600 mg/kgBB), ethanolic extract of Curcuma aeruginosa (600 mg/kgBB), and combination ethanolic extract of Curcuma zedoaria and Curcuma aeruginosa at dosage of 150:450 mg/kgBB, 300:300 mg/kgBB, and 450:150 mg/kgBB. Tonic effect were evaluated using natatory exhaustion and rotarod tests. The natatory exhaustion test is carried out by observing how long the mice swim, while the rotarod test is carried out by observing how long the mice stay on the rotarod apparatus. The data analyzed using ANOVA and Tukey’s test. The results of One Way Anova showed a there were significansts differensce in each groups (p<0,05) with combination of Curcuma zedoaria : Curcuma aeruginosa (150:450 mg/kgBB) yielding the best tonic effect. From the result of Tukey’s followsup test, it showed that the highers the dosae of Curcuma aeruginosa rhizome extracts the better the tonics effects giiven. This suggest that Curcuma aeruginosa has a dominant role in enhancing phsycal endurance in male white mice.

Memory is a vital biological system in the body that plays a role in storing, processing, and recalling information. Over time, due to aging or external factors, memory function may decline. One possible effort to maintain and improve cognitive performance is through the use of bioactive compounds with antioxidant properties. Avocado (Persea americana Mill.) leaves contain flavonoids, which act as antioxidants and possess neuroprotective effects that may enhance cognitive function, including memory. This study aimed to determine the effect of avocado leaf extract on the memory of male mice and to identify the most effective dose in improving cognitive performance. The extract was obtained using the maceration method with 70% ethanol as the solvent. Memory activity was tested on male mice, which were divided into five groups: negative control, positive control, and three treatment groups given avocado leaf extract at doses of 50, 100, and 200 mg/kgBW. The Y-Maze method was employed to assess memory through two parameters: latency time and error numbers. The results demonstrated that avocado leaf extract significantly improved memory in the test animals. Mice induced with 10% ethanol showed an increase in latency time and error numbers, while the groups treated with avocado leaf extract experienced reductions in both parameters. The most effective dose was 200 mg/kgBW, which produced a latency time difference of 19.5 seconds and an error number difference of 3.57. Therefore, avocado leaf extract shows potential as a natural agent to support and enhance cognitive function.

Dyslipidemia is a lipid metabolism disorder characterized by an increase or decrease in blood plasma fat levels. This condition includes high levels of total cholesterol, low-density lipoprotein (LDL), and triglycerides, as well as decreased levels of high-density lipoprotein (HDL). In general, dyslipidemia is divided into two types: primary, which is influenced by genetic factors, and secondary, which is caused by other diseases or an unhealthy lifestyle. Dyslipidemia is one of the major risk factors for cardiovascular disease, making its prevention and treatment very important. In this study, male white mice were used and induced with a high-fat diet to trigger dyslipidemia. The test animals were then divided into six groups: a negative control, a positive control (given simvastatin at a dose of 0.36 mL/day), and three treatment groups with microcapsules of Chinese betel leaf herb ethanol extract (EEHSC) at concentrations of 0.25%, 0.5%, and 0.75%. The treatment was administered orally for 14 days. Cholesterol levels were measured before and after treatment using a digital cholesterol tester and laboratory analysis to ensure more accurate results. The results showed a decrease in cholesterol levels with varying percentages in each group. The negative control decreased by 24.8%, the positive control by 36.1%, the 0.25% EEHSC by 24.1%, the 0.5% EEHSC by 34.7%, and the 0.75% EEHSC by 45.5%. This indicates that the 0.5% EEHSC treatment produced cholesterol reductions almost comparable to simvastatin. Interestingly, the highest effect was observed in the 0.75% group, indicating a dose-dependent response. In contrast, the 0.25% concentration only produced a less than optimal effect. Thus, microcapsules of ethanol extract of the Chinese betel leaf herb have potential as an alternative agent for lowering cholesterol levels, particularly in dyslipidemia. These findings suggest that natural-based therapies could complement conventional treatments in managing lipid disorders.

Epilepsy is a chronic neurological disorder characterized by recurrent seizures due to abnormal electrical activity in the brain. This condition can significantly impact the quality of life of sufferers, making the development of effective and safe anticonvulsant therapies a priority in pharmacological research. This study aimed to evaluate the effectiveness of various anticonvulsant drugs, including herbal candidates, in a mouse model (Mus musculus) using acute and subchronic test protocols. Seizures were induced by administering strychnine, a glycine receptor antagonist that triggers excessive neuronal excitability. Parameters observed included seizure onset (the time from strychnine administration to the onset of the first seizure) and deadtime (the time of survival after a seizure). The study involved six treatment groups: a negative control (Na-CMC), four conventional drug groups (phenytoin, diazepam, pregabalin, and gabapentin), and one herbal candidate group made from Polyalthia longifolia (glodokan tiang). The results showed that most treatment groups were able to delay seizure onset and significantly prolong deadtime compared to the negative control. Statistical analysis using ANOVA yielded a p-value <0.05, indicating a significant difference between groups. The herbal candidate Polyalthia longifolia showed promising anticonvulsant potential, although it did not fully match the effectiveness of conventional drugs such as phenytoin or diazepam. The negative control (Na-CMC) showed the fastest onset and shortest survival time, reflecting the absence of a protective effect against seizures. This study emphasizes the importance of further testing using chronic protocols and evaluating additional parameters such as plasma drug concentrations and neurological side effect profiles. The obtained data are expected to form the basis for the development of anticonvulsant therapies based on a combination of modern drugs and natural ingredients, with the hope of producing safer, more effective treatment alternatives, and potentially reducing dependence on long-term synthetic drugs.

Indonesia is recognized as one of the world’s richest countries in terms of biodiversity, including a wide variety of traditional medicinal plants. One of the lesser-known yet promising local species is matoa (Pometia pinnata J.R. Forst & G. Forst), whose leaves have been traditionally used by local communities to treat various ailments, including diarrhea and seizures. This study aims to investigate the pharmacological effects of matoa leaf extract, with a particular focus on its anticonvulsant activity, while also reviewing its potential chemical constituents as a basis for herbal medicine development. The research was conducted using a combination of literature review and experimental testing on mice (Mus musculus) induced with strychnine to trigger seizures. Key observed parameters included seizure onset time and survival duration (dead time) following the administration of either synthetic drugs or matoa leaf extract. The results demonstrated that the matoa leaf extract exhibited significant anticonvulsant effects, as indicated by prolonged seizure onset and increased survival time in test animals. These pharmacological effects are presumed to be related to the presence of active compounds such as flavonoids, tannins, and alkaloids, which may contribute to the stabilization of the central nervous system. Compared to conventional synthetic anticonvulsants, matoa leaf extract may offer a safer, plant-based alternative with potentially fewer side effects. The findings of this study provide valuable scientific evidence supporting the potential of Pometia pinnata leaves in the development of Indonesian herbal phytopharmaceuticals. Furthermore, they highlight the importance of further research, including preclinical and clinical trials, to validate efficacy, determine optimal dosages, and ensure safety for human use.

Excessive fat consumption can lead to increased cholesterol levels in the body, which in turn can trigger various serious health problems, including heart disease, blood vessel blockage, and even stroke. Blood cholesterol consists of two main types: LDL (Low-Density Lipoprotein) cholesterol, often referred to as bad cholesterol, and HDL (High-Density Lipoprotein) cholesterol, which plays a role in transporting cholesterol from the blood vessels to the liver for excretion. Elevated LDL cholesterol levels can cause plaque buildup on blood vessel walls, which narrows blood flow and increases the risk of various cardiovascular diseases. One method considered effective for lowering LDL cholesterol levels in the blood is by consuming phytosterols, compounds found naturally in various types of plants. Phytosterols have a structure similar to cholesterol and can compete with cholesterol for absorption in the intestine. This results in a decrease in the amount of cholesterol entering the body, thereby reducing total cholesterol levels and especially LDL cholesterol in the blood. Research shows that giving food or supplements containing about 3 grams of phytosterols per day to human subjects can reduce LDL cholesterol levels by up to 15%. Furthermore, phytosterols directly isolated from plants, such as green beans, have also been shown to significantly lower LDL cholesterol levels. Studies in mice fed phytosterols isolated from green beans showed a reduction in blood LDL cholesterol levels of more than 30%. This demonstrates the potential of phytosterols as a natural ingredient that can be used to support cholesterol management in the body. Although phytosterols have significant potential in lowering cholesterol levels, it is important to remember that consuming supplements or foods containing phytosterols should be done under the supervision and recommendation of a medical professional, especially for individuals with certain medical conditions or who are taking medications.  

Pain is an unpleasant sensation that can interfere with an individual's quality of life and requires effective management. Synthetic analgesics such as ketorolac, codeine, etoricoxib, and celecoxib are commonly used to relieve pain, but long-term use can cause adverse side effects, including gastrointestinal disturbances, dependence, and cardiovascular risks. Therefore, it is important to find safer, natural alternatives. One promising alternative is the extract of the dragon’s tail leaf (Rhaphidophora pinnata), a plant traditionally used in Indonesian medicine as a pain reliever. This study aims to evaluate and compare the analgesic effects of Rhaphidophora pinnata leaf extract with several synthetic drugs using the writhing test on male mice (Mus musculus). The mice were induced with pain by injecting a 0.5% acetic acid solution. The treatments included ketorolac, codeine, etoricoxib, celecoxib, and Rhaphidophora pinnata leaf extract. The results showed that all treatments, including both synthetic drugs and Rhaphidophora pinnata leaf extract, produced significant analgesic effects compared to the negative control (Na CMC 0.5%). Ketorolac showed the highest effectiveness, followed by codeine, etoricoxib, and celecoxib. Although the Rhaphidophora pinnata leaf extract showed potential as a natural analgesic, its effectiveness varied among individual test animals. Some mice showed a better response to the leaf extract, but overall, the analgesic effect was still lower than that of synthetic drugs. These findings support the use of medicinal plants as a safer alternative to synthetic analgesic drugs. Moreover, this study provides a foundation for further research aimed at isolating active compounds from Rhaphidophora pinnata leaf extract to develop more effective and safer pain-relieving medications.

Pharmacology is the study of drug interactions with living organisms. This study aims to evaluate the anti-inflammatory effects of several drug preparations, including Hemilon (methylprednisolone), Lupred (prednisolone), Kamathasone (dexamethasone), neem leaf infusion, and snakehead fish extract, on mice (Mus musculus) induced with inflammation using carrageenan solution via intraplantar injection. Edema volume was observed using a plethysmometer at 15, 30, 45, and 60 minutes post-induction. Data were analyzed using normality, homogeneity, ANOVA, and Tukey tests via SPSS. Results showed that Hemilon had the highest anti-inflammatory effect, followed by Kamathasone and Lupred, while neem leaf and fish extract showed weaker activity. Statistical tests revealed normal distribution (sig. 0.197 > 0.05), non-homogeneous data (sig. 0.022 < 0.05), and no significant difference between groups (ANOVA sig. 0.366 > 0.05). Despite this, descriptive results confirmed strong anti-inflammatory potential of synthetic drugs. This practicum provides valuable insights into drug mechanisms and emphasizes methodological accuracy in pharmacological testing.

CBT Room 2 at the Faculty of Medicine, Universitas Muhammadiyah Purwokerto, is used for computer-based lectures and practicums, which may lead to exposure to pathogenic microorganisms from device surfaces, such as computer mice. This study aims to identify pathogenic bacteria and fungi present on mouse surfaces and to evaluate the influence of room temperature and humidity on microbial growth. A total of 60 mouse samples were tested using Nutrient Agar (NA) for bacteria and Sabouraud Dextrose Agar (SDA) for fungi. The recorded room temperature was 25.6°C with a humidity level of 74%. The identification results revealed the presence of Bacillus sp., Escherichia coli, Staphylococcus sp., and Streptococcus sp. on NA media, with Gram-positive bacteria dominating (78.33%). Meanwhile, Aspergillus sp. (40.00%) and Penicillium sp. (38.33%) were found on SDA media. These findings indicate that, although the room's temperature and humidity are not at optimal levels, they still support the growth of mesophilic microbes. The implications suggest the need for routine sterilization efforts to prevent cross-infection risks in the learning environment.  

The development of the Mice Industry in Indonesia is growing, this can be seen in 2023 as many as 97.86% of event organizers have returned to being held. The purpose of this study is to measure and analyze the PRO Audio Visual, LED, Lighting & Music Indonesia 2024 Event, the image of PT. Kristamedia Pratama at the PRO Audio Visual, LED, Lighting & Music Indonesia 2024 event, the influence of the PRO Audio Visual, LED, Lighting & Music Indonesia 2024 event on the image of PT Kristamedia Pratama. The theory used for the event is a means for the company as a public relations to introduce and communicate the brand to potential consumers involved in the event and the company's image is a form of intangible asset in a company that has a uniqueness that is easy to identify but difficult to imitate. The research approach uses quantitative. The research method uses a survey. The data collection technique uses simple random sampling with a research sample of 100 respondents. The data collection method uses a questionnaire. The data analysis methods used are validity testing, reliability testing, frequency analysis, correlation analysis, simple linear regression analysis. The results of the study show that events have an overall average value of 4.03, while corporate image has an overall average value of 4.09. Events have a positive and significant effect on corporate image. The r-square value in the coefficient of determination test shows that events have a 45.4% effect on corporate image.

Wounds are damage to body tissues caused by various factors, both external and internal, which can disrupt the normal function of these tissues. Many studies have focused on using natural ingredients as adjunctive therapy to accelerate wound healing. One plant that shows therapeutic potential is Garcinia mangostana L. This study aims to assess the therapeutic potential of Garcinia mangostana L. stem bark and fruit peel extracts in accelerating the healing of mice (Mus musculus) incision wounds. The study used a completely randomized design (CRD) with six treatments and three replicates, namely negative control mice (K-), positive control (K+), groups of mice given fruit peel and stem bark extracts of G. mangostana concentrations of 25, 50, 75 and 100%. All mice were cut with a wound diameter of 1 cm. The wounds were then left for 13 days with an interval of 3 days for measurement. The results showed that the administration of G. mangostana fruit peel and stem bark extracts had an effect in accelerating the healing of mice incision wounds. From these results, Garcinia mangostana L. has the potential as an additional therapy in accelerating wound healing in mice, which can be used as a basis for further research related to its application in humans.

Inflammation is a response to tissue damage due to various adverse stimuli, both chemical and mechanical stimuli and infections. One of the chemical compounds found in henna leaves is flavonoids that are efficacious as anti-inflammatory. The aim of the study was to determine the anti-inflammatory effectiveness of water henna leaf extract reviewed from a decrease in udem volume in the soles of the feet of male white rats induced by carrageenan 1%. The research method used was experimental on simplicia and ethanol extract of henna leaf (EEDPA) as well as testing the anti-inflammatory effectiveness of EEDPA. Testing of the anti-inflammatory effect was carried out on 30 male white mice induced with 1% carrageenan subplantarly. The test animal group was divided into five groups consisting of a positive group given Na-diclofenac, a negative control group given CMC, and a test group of water henna leaf ethanol extract at doses of 200 mg/kgBB, 400 mg/kgBB and 800 mg/kgBB. The parameters observed in this study were the volume of the leg udem of the test animal which was measured with a Plethysmometer every 6 hours. The data obtained was then calculated as the percent of inflammation and the percent of inflammation inhibition. Analyzed with One Way ANOVA with a 95% confidence rate. The results of the effectiveness test showed that EEDPA had an anti-inflammatory effect in carrageenan-induced male white rats. Optimal effectiveness indicated by the dose showed that EEDPA at a dose of 800 mg/kgBB

Diabetes mellitus is a chronic metabolic disorder characterized by hyperglycemia due to insulin deficiency or insulin resistance. Currently available pharmacological treatments often cause unwanted side effects, so alternative therapies that are safer and more effective are needed. This study aims to analyze the effectiveness of telang flower extract in reducing blood glucose levels and preventing complications. The potential of Clitoria ternatea as an antidiabetic agent was explored through a systematic literature review, using scientific databases such as Google Scholar, ScienceDirect, and Mendeley with the keywords “Clitoria ternatea AND diabetes AND blood glucose.” Inclusion criteria applied included experimental studies conducted on diabetes-induced mice samples, interventions with telang flower extract administration, and publications within the last 5 years (2021-2025). From a total of 1,858 articles identified, by applying the PRISMA-ScR method, 8 articles were obtained that met the inclusion criteria. The analysis showed that telang flower extract was effective in reducing blood glucose levels. This antidiabetic effect is thought to be due to the presence of flavonoids and other bioactive compounds in telang flowers that act as α-amylase enzyme inhibitors, antioxidants, and anti-inflammatories. These findings indicate the potential of Clitoria ternatea as a promising therapeutic alternative in the management of diabetes mellitus.

Indonesia, considered the country with the second-highest level of biodiversity in the world after Brazil, possesses extraordinary biological wealth that remains underutilized. The field of traditional medicine utilizes natural resources to prevent and treat physical and mental disorders. Periwinkle, or Catharanthus roseus, is one of the most widely used plants due to its abundance of bioactive phytochemicals, such as alkaloids, flavonoids, saponins, and tannins. The ethanol extract from Periwinkle leaves has the potential to act as a natural antipyretic agent that can reduce body temperature during fever. The purpose of this study is to review and analyze previous research findings on the antipyretic effects of ethanol extract from Periwinkle leaves on mice (Mus musculus). According to a literature review from various sources, the ethanol extract of Periwinkle leaves demonstrates a comparable level of effectiveness to synthetic antipyretic drugs in lowering the body temperature of fever-induced mice. Furthermore, the content of active compounds and the antipyretic performance of this plant are influenced by its variety and environmental factors. Body temperature begins to decrease one hour after extract administration and reaches its peak reduction at the third hour. Therefore, Periwinkle may serve as an alternative natural antipyretic remedy. However, further research is necessary to standardize its usage and conduct clinical trials on humans.

This study aims to analyze the forms and structure of social stratification portrayed in the animated film Zootopia, produced by Walt Disney Pictures. The film was chosen because it represents societal structures with various social layers through animal characters that reflect human social realities. The type of research used is descriptive qualitative research, with data collection techniques involving observation and note-taking of scenes, dialogues, and narratives that illustrate social class divisions in the film. The study was conducted objectively without comparing it to the real-life society where the film was produced. The theoretical framework used in this study is based on Sorokin's theory, which divides society into three social classes: upper class, middle class, and lower class. Additionally, Soekanto's theory is applied, which classifies social stratification into closed, open, and mixed classes.The results of the study show that Zootopia presents a clear picture of social stratification. The upper class is represented by predators, such as lions and wolves, who hold power and have influential roles in the government. The middle class is symbolized by wealthy mice involved in mafia activities. The lower class consists of herbivores, who are perceived as weak, often discriminated against, and have limited access to power and resources. The film also illustrates an open class system, where certain characters from the lower class—such as Judy Hopps—are able to transcend social boundaries and achieve higher status through effort and merit.

Pain is the body's response to tissue damage that is often addressed using analgesic compounds. Purslane plants (Portulaca oleracea L) are known to contain flavonoids, saponins, tannins, and alkaloids that have potential as analgesics. This study aims to test the analgesic effectiveness of purslane ethanol extract by writhing test method using white mice (Mus musculus) induced by 1% acetic acid. A total of 15 mice were divided into 5 treatment groups, namely negative control, positive control (paracetamol), and three groups with doses of purslane extract 200 mg/kgBB, 400 mg/kgBB, and 800 mg/kgBB. The number of writhes was observed every 15 minutes for 1 hour, and the percentage of protective power was calculated. The results showed that the dose of 800 mg/kgBB gave the highest analgesic effect with a percent protection power of 65.28%, followed by doses of 400 mg/kgBB (52.4%) and 200 mg/kgBB (30.6%). The highest analgesic effect was achieved at a dose of 800 mg/kgBB, almost equivalent to the positive control. This shows the significant potential of purslane ethanol extract as an analgesic agent.