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The high mortality in crayfish shipping transportation activities is due to the high level of stress during transportation. The handling that needs to be considered is when harvesting and transporting the fry. Poor seed transportation can result in stress to high mortality. Transportation of live crayfish can be done in two ways, namely with a wet system using water and a dry system without using water media. Research to maintain mortality begins with packing procedures starting from placing ice cubes in plastic that has been wrapped in newspaper in each 500ml seteroafoam and then inserting 22 crayfish in each box and then allowed to stand with a predetermined deadline. The first treatment survival rate reached 97% of 66 mortalities of 2 tails, in the second treatment 94% of 66 mortalities of 4 tails, the third treatment 80%, of 66 mortalities of 9 tails. Based on the results of the study, the addition of ice cubes to the packing media for crayfish with living conditions resulted in an average survival rate of 90.3% with the shortest deadline of 30 hours and the longest of 40 hours, thus it can be said that the packing media with the addition of ice cubes has an impact on the survival rate of crayfish.

Ice cubes are a food product that is often consumed by the community. Ice cubes are usually mixed in drinks to add freshness. Ice cubes are made from drinking water frozen at 0°C, although low temperatures can inhibit the growth of microorganism cells, ice cubes still have the possibility of containing contaminant microorganisms. Ice cubes that can cause diarrhea are ice cubes that contain pathogenic bacteria, one of which is Escherichia coli. Therefore it is necessary to check the quality of ice cubes microbiologically. This study aims to detect the presence of Escherichia coli in ice cubes sold by food stalls in the Liliba Village, Kupang. This type of research uses a descriptive method, where the population in this study is ice cubes which are sold in 10 different stalls in Liliba Kupang Village. The method used is Most Probable Number (MPN) with a variance of 5-1-1 followed by Gram staining and IMVIC test. From the results of the study, it can be found that ice cubes are sold in food stalls in Liliba Kupang Village, from 10 samples of ice cubes there is 1 sample that contains 10% Escherichia coli bacteria.