Publication Search

This study aims to determine the effect of Lactobacillus casei and Ambon banana flour on the viability of lactic acid bacteria and inhibition of pathogens in yogurt. The studies used the Randomized Block Design (RBD) 2 factor, starter type and ambon banana flour concentration. The starters type consisting of Streptococcus thermophillus and Lactobacillus bulgaricus; S. thermophilus, L. bulgaricus and L. casei.  The banana flour concentration consisting of 0; 2.5; 5.0; 7.5 and 10%. Analyzed used one-way ANOVA and continued with Duncan's Multiple Range Test. Variables observed including the viability of lactic acid bacteria, inhibition of pathogen (Escherichia coli and Staphylococcus aureus), and yogurt preference. The results showed that the addition of L. casei to yogurt cultures decreased the viability of lactic acid bacteria and inhibition of E. coli but increased inhibition of S. aureus. Increasing the concentration of banana flour causes increased viability of lactic acid bacteria, inhibiting against E. coli and S. aureus. The highest lactic acid bacteria viability has resulted in 10% banana flour concentration. Yogurt without the addition of banana flour cannot inhibit E. coli and S. aureus. Increasing the concentration of banana flour added to yogurt causes a decrease in preference.

Batang gaharu (Aquilaria malaccensis L.) merupakan salah satu tanaman yang dapat dimanfaatkan sebagai antibakteri yang diperlukan tubuh untuk melawan bakteri patogen. Potensi antibakteri yang dimiliki batang gaharu disebabkan adanya kandungan senyawa kimia didalamnya.  Penelitian ini bertujuan untuk mengidentifikasi kandungan senyawa kimia batang gaharu (Aquilaria malaccensis L.) serta mengetahui zona hambat dari antibakteri ekstrak etanol batang gaharu (Aquilaria malaccensis L.) yang dilakukan dengan menggunakan metode difusi sumuran. Pada penelitian ini dilakukan uji skrining fitokimia menggunakan pelarut etanol 96%. Ekstraksi batang gaharu dilakukan dengan menggunakan metode maserasi. Hasil skrining fitokimia ekstrak etanol batang gaharu (Aquilaria malaccensis L.) menunjukan adanya senyawa flavonoid, alkaloid, dan terpenoid. Hasil uji efektifitas antibakteri ekstrak etanol batang gaharu  (Aquilaria malaccensis L.) terhadap pertumbuhan bakteri Staphylococcus aureus diperoleh konsentrasi 15% dengan diameter zona hambat 11 mm, konsentrasi 30% dengan diameter 13 mm, dan konsentrasi 60% dengan diameter 14 mm.

Sea urchin (Diadema setosum) is an aquatic biota that has high nutritional value. Bioactive compounds produced by sea urchins have the potential to be used as natural antibacterial compounds. This study aims to identify chemical compounds and determine the antibacterial activity of ethanol extract of sea urchins (Diadema setosum) against the growth of Staphylococcus aureus bacteria. The research method used is the agar diffusion method. The results of phytochemical screening of sea urchin extract (Diadema setosum) showed the presence of alkaloids and saponins. By using variations in the concentration of ethanol extract of sea urchin (Diadema setosum) at a concentration of 80% it had an inhibitory power of 18 mm, a concentration of 90% had an inhibitory power of 23 mm, a concentration of 100% had an inhibitory power of 28 mm and for a concentration of 110% had an inhibitory power of 35 mm. The positive control has a resistance of 30 mm and the negative control is 0 mm. The results showed that the ethanol extract of sea urchins (Diadema setosum) had a strong bacterial inhibitory activity against Staphylococcus aureus bacteria.

Labu kuning (Cucurbita moschata) merupakan sayuran penting karena nilai nutrisinya dan manfaat kesehatannya. Tanaman ini adalah sumber karotenoid yang kaya akan vitamin larut air, fenolat, flavonoid polisakarida, garam mineral, dan vitamin yang semuanya bermanfaat bagi kesehatan. Buah labu kuning mempunyai kulit yang sangat tebal dan keras, sehingga dapat bertindak sebagai penghalang laju respirasi, keluarnya air melalui proses penguapan, maupun masuknya udara penyebab proses oksidasi.. Tujuan dari pada penelitian ini adalah untuk mengetahui aktivitas antibakteri ekstrak biji labu kuning (curcubita moschata) terhadap daya hambat bakteri staphylococcus aureus dengan metode Difusi sumuran. Jenis penelitian ini adalah True Eksperimental laboratorium dengan menggunakan metode  difusi sumuran. Sebagai control positif yang di ganakan adalah kloramfenikol. Selanjutnya diinkubasi pada temperatur 37o selama 24 jam yang kemudian zona hambat yang terbentuk selanjutnya diukur. Hasil dari penelitian ini adalah ekstrak biji labu kuning (curcubita moschata) memiliki aktivitas antibakteri dengan adanya daya hambat disekitar sumuran dari masing-masing konsentrasi ekstrak. Pada konsentrasi ekstrak 10% diameter daya hambat 19 mm, konsentrasi 15% diameter daya hambat 20 mm, konsentrasi  20% diameter daya hambat 21 mm dan untuk konsentrasi 15% dengan diameter daya hambat 24 mm, sehingga dapat disimpulkan bahwa ekstrak biji labu kuning (curcubita moschata.) memiliki aktivitas antibakteri terhadap bakteri staphylococcus aureus.

Nutmeg is known as a spice plant that has economic value and multipurpose because every part of the plant can be used in various industries. Compounds contained in nutmeg seeds include essential oils, a-pinene, b-pinene, limonene, myristicin, safrole, and methyl eugenol. Nutmeg plant nutritious as antidiarrheal, antidiabetic, antimicrobial. The purpose of this study was to make a formulation of rubbing oil and to determine the bacteriostatic activity of nutmeg seed oil on staphylococcus aureus bacteria. This study used three formulations, namely the provision of distilled water as a negative control, chloramphenicol as a positive control. The method used in this research is the well method. Bacteria that were incubated for 24 hours in the fastest concentration inhibition research were at 50% concentration because of bacterial growth which showed that the higher the concentration, the higher the inhibitory power and at 20% concentration the results were resistant, because the amount of solvent used was higher. low and can not inhibit the growth of microorganisms.