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Critical thinking ability is one of the important competencies that students must possess in biology learning. This research aims to develop Problem-Based Learning (PBL) Student Worksheets to train students' critical thinking skills in Plant Anatomy courses. This research uses the Research and Development method with a simplified 4-D model consisting of three stages: Define, Design, and Develop. The research subjects were second-semester Biology Education Study Program students at Universitas Muhammadiyah Bengkulu. Research instruments included media and material expert validation sheets, as well as student response questionnaires. Validation was conducted by one media expert and one material expert, then tested on 5 students (limited trial) and 22 students (extensive trial). Validation results showed that the student worksheets received a "very valid" category with 86% from media experts and 88% from material experts. Practicality tests showed "very practical" results with 89% in limited trials and 91% in extensive trials. The developed student worksheets contained contextual problem scenarios, systematic PBL stages, and critical discussion questions that encourage students to analyze, evaluate, and conclude based on empirical data. This research concludes that PBL-based student worksheets are feasible to use as learning media to train students' critical thinking skills in Plant Anatomy courses.

Biology education in high school has an important role in shaping students' science literacy and critical thinking skills. However, ecological material, particularly the topic of Ecosystem Components and their Interactions, is often considered difficult because it is abstract and not easy to observe directly. This research aims to improve student learning outcomes through the application of the Problem Based Learning (PBL) model, which emphasizes real problem-solving, collaborative discussion, and active involvement in the learning process. The research uses the design of Classroom Action Research (PTK) which was carried out in two cycles in class X of St. Rosa de Lima Catholic High School Tondano involving 10 students in the odd semester of the 2025/2026 school year. Data was collected through observation of teacher and student activities, learning outcome tests in the form of pretests and posttests, and learning documentation. The analysis was carried out descriptively, qualitatively, and quantitatively by calculating the average score, percentage of completion, and student involvement. The results of the study showed a significant increase. The percentage of teacher activity increased from 58.3% in the first cycle to 87.5% in the second cycle, while student activity increased from 45.83% to 83.33%. The average score of students also increased from 70% in cycle I to 90% in cycle II, with classical completeness reaching 90%. These findings confirm the effectiveness of PBL as a contextual Biology learning strategy, able to bridge ecological concepts with real life, and increase students' curiosity, cooperation, and critical thinking skills.

This classroom action research evaluates the effectiveness of Problem Based Learning in learning Biology on the topic of environmental change at Rosa de Lima Catholic High School Tondano, with the aim of improving the activities and learning outcomes of grade X students. The method used is two action cycles with stages of planning, implementation, observation, and reflection. The subjects are 10 students. Tools include Teaching Modules and Student Worksheets; Data was collected through observation sheets of teacher and student activities (scales 1–4) and 10-item learning outcome tests each cycle. The descriptive analysis calculates the grade average and classical completeness against the Minimum Completeness Criterion of 75. The results showed consistent improvement from cycle I to cycle II. Classical completeness increased from 66% (6 out of 9 students attended; average 76.67) to 90% (9 out of 10 students; average 81.00). The teacher's activity observation score increased from 3.75 to 3.91, while the student's activity from 3.50 to 3.60. The increase is fueled by the formulation of contextual problems, facilitation of guiding questions, individualized feedback, and more disciplined time management, which strengthens engagement, collaboration, and scientific reasoning. The findings indicate that Problem Based Learning is effective in improving Biology learning activities and outcomes on the topic of environmental change. This study adds to the empirical evidence in the context of high schools with low initial completeness and suggests further exploration of other Biology topics along with measurements of critical thinking skills and scientific attitudes for a more comprehensive picture of impact.

Literacy skills play an important  role in helping students clarify basic concepts, build relationships between concepts, reason and test each detail of each interrelated information and are able to derive each concept, thus students are accustomed to understanding each scientific process and activity carried out in Biology learning. The relationship between these two skills trains students to actively carry out in-depth scientific investigations.The purpose of this study was to identify the relationship between scientific literacy skills and scientific explanation skills in empowering scientific reasoning skills through the application of the Remap-NHT (Reading Concept Map-Numbered Head Together) learning model. This study included a quasi-experiment using a non-equivalent pretest-post-test control group design with a total sample of 85 eleventh grade students in the Natural Sciences department at SMAN 4 Malang in the 2023/2024 academic year. The population was distributed into 3 groups. Scientific literacy skills and scientific reasoning skills were assessed using an essay test, while scientific explanation skills were assessed using an assessment rubric. Data analysis was tested using multiple regression analysis. The results showed that (1) there was a significant relationship between scientific literacy skills and scientific reasoning skills; (2) there is a significant relationship between scientific explanation skills and scientific reasoning skills; and (3) there is a significant relationship between scientific literacy skills and scientific explanation skills that have an integral effect on scientific reasoning skills. The interrelationship between scientific literacy skills, scientific explanation skills and scientific reasoning skills can be observed in the regression equation Y = 0.317 * X1 + 0.995 * X2 + 30.113. Based on the findings, it is concluded that Remap-NHT is able to improve students' scientific literacy skills, scientific explanation skills, and scientific reasoning skills, the interrelationships of which influence each other.

The topics of morphology, anatomy, and physiology are integral to biology learning, yet they often present challenges for students due to their abstract and complex nature. Difficulties in understanding body structures and invisible biological processes lead to low student interest and engagement. This study aims to identify innovative learning approaches through an analysis of national scientific literature over the past decade. The results show that the integration of digital technology and student-centered learning methods can improve conceptual understanding and learning motivation. The most prominent innovations include the use of VR and AR-based media to clarify visualizations, the use of interactive videos and simulations to explain biological functions, and the application of contextual learning models such as Problem-Based Learning (PBL) and Project-Based Learning (PjBL). These approaches have proven effective in creating a more immersive learning experience that is relevant to students' needs.

Improving the quality of biology learning in secondary schools requires pedagogical innovations that connect academic content with real-life contexts and leverage digital technology. This study aims to analyze the effect of a contextual teaching approach assisted by interactive PowerPoint on students’ learning outcomes in the human respiratory system topic. A true experimental design with a pretest–posttest control group was employed at SMA Negeri 1 Langowan, involving two eleventh-grade classes selected through simple random sampling. The experimental class received contextual instruction integrated with interactive PowerPoint, while the control class was taught using conventional methods. Data were collected through validated achievement tests and analyzed using normality and homogeneity tests, followed by independent and paired t-tests. The results indicate a significant improvement in learning outcomes for students in the experimental class compared to the control group. The experimental group’s posttest scores were substantially higher, and the difference was statistically significant. Integrating a contextual approach with interactive visualization effectively enhanced students’ understanding of abstract biology concepts, promoted active participation, and strengthened learning motivation. These findings support constructivist theory and highlight the relevance of contextual teaching in science education aligned with the Merdeka Curriculum. The study implies the importance of implementing context-based strategies combined with simple digital media to improve biology learning effectiveness. Future research may explore its impact on non-cognitive skills such as critical thinking and scientific attitudes. Furthermore, the results of this study provide opportunities for teachers to be more creative in designing teaching materials that are not only informative but also interactive and relevant to students' lives. By utilizing media such as interactive PowerPoint, teachers can present complex biological content in a more engaging and understandable way.

The purpose of this study is to determine the pattern of the concept of integration between Science and Islam, to explore how this integration is implemented, and to identify the factors that influence its effectiveness in the context of learning for the Integrated Madrasah Science Competition (KSM) in Biology at MAN 1 Wonosobo. This study employs a qualitative research method, utilizing field research and descriptive research approaches. The findings indicate that the pattern of integration between Science and Islam in biology learning is interconnective, blending both perspectives seamlessly. Supervisory teachers, who predominantly have a pure scientific background in biology, play an active role in discussions and studies. The use of biology textbooks as learning resources, which integrate both scientific and Islamic content, is maximized, allowing for in-depth discussions between teachers and students. Key factors influencing the effectiveness of the Biology KSM include the curriculum and learning materials that emphasize the integration of Science and Islam. The competencies of interdisciplinary teachers who understand and master the integration of both sciences are crucial for the success of the Biology KSM. Additionally, support from all components of the madrasah is essential for creating a motivating and guiding framework that encourages the integration process. These findings suggest that the combination of academic expertise in both science and Islam, along with strong institutional support, contributes significantly to the effectiveness of the Integrated Madrasah Science Competition and enhances the learning experience for students. This study provides insights into how the integration of Science and Islam can be effectively implemented in the educational context, specifically within biology education.

Virgin Coconut Oil (VCO) is a processed coconut product with high economic and health value. VCO production is known to be relatively easy, inexpensive, and capable of producing high-quality oil rich in benefits, including as a traditional medicine and natural ingredient for beauty and health. This community service activity aims to provide students of the Biology Education Study Program at Buton Muslim University with an understanding and practical skills regarding the VCO production process as a form of utilization of local natural resources, especially coconuts. The methods used in this activity include lectures or delivery of theoretical material, interactive discussions on the content and benefits of VCO, and direct practice in making virgin coconut oil using the settling method. This method was chosen because it does not require complicated equipment, does not use excessive heating, and is able to maintain the natural quality of the resulting oil. During the implementation, students are invited to observe and follow the entire process, from selecting good coconut raw materials, the grating process, squeezing coconut milk, natural fermentation through settling, to the process of separating oil from sediment and filtering. The results of this activity demonstrated that students not only understood the concept and theory of VCO production but also produced 1,500 ml of virgin coconut oil packaged in five 300 ml bottles. The VCO was then sold to the public for approximately Rp 30,000 per bottle. Through this activity, students not only gained contextual learning experiences but also developed insights into sustainable, locally-based entrepreneurship.

This study aims to observe the phases of mitosis in the meristem cells of shallot roots (Allium cepa) through microscopic observation as part of the Cell Biology learning model. Mitosis is an important part of the cell cycle that plays a role in growth, tissue repair, and asexual reproduction in organisms. A deep understanding of the mitosis process is an important foundation in various branches of biology, including genetics, biotechnology, and histology. Shallot roots were chosen because they have actively dividing meristem tissue at the root tip,as well as a large and transparent cell structure that facilitates the staining and observation process. Preparation was carried out by soaking the onion roots in a solution of acetic acid and alcohol for fixation,then staining with acetocarmine acid so that the chromosomes are clearly visible. The process of crushing the root tip and pressing it on a glass object (squash method) was used to expand the observation area. A light microscope with a magnification of 400x and 1000x was used to identify the division phases. Observation results show the presence of all phases of mitosis, namely prophase (nucleolus and nuclear membrane begin to disappear,chromosomes condense), metaphase (chromosomes line up at the equator), anaphase (chromatids separate to opposite poles), and telophase (nuclear membrane and two daughter cell nuclei re-form). Visual documentation in the form of microscopic photographs and descriptive tables clarify the identification of these phases, thus facilitating student understanding.From a pedagogical perspective, this microscopic practicum-based learning has been proven to improve students' observational skills, laboratory skills, and conceptual understanding of cell division. Students not only memorize theories, but directly experience the scientific process through exploration and discussion of observation results. Evaluation of learning outcomes is carried out through post-practicum quizzes and student worksheets, showing an increase in understanding scores of more than 30%.

The research conducted in class X IPA SMA Negeri 1 Rengat aims to determine the level of student learning difficulties in biology subjects in class X IPA SMA Negeri 1 Rengat. This study used a cluster sampling sample with a population of 90 students of class X IPA. Data analysis in this study used percentage techniques. The results of the study showed that the level of learning difficulties of students at SMA Negeri 1 Rengat, physiological indicators were 56.4% in the sufficient category, psychological indicators were 65.26% in the sufficient category, family indicators were 66.01% in the sufficient category, school indicators were 59.46% in the sufficient category, community indicators were 62.5% in the sufficient category, the average overall percentage was 61.92% in the good enough category.

This study aims to determine how active students are in learning online biology subjects during the Covid-19 outbreak. This research uses a descriptive method and the type of research is survey. The population is students of class XI IPA of SMA Negeri 3 Panyabungan and the sample students of class XI IPA 2 and XI IPA 3. Meanwhile, the research subjects used biology teachers and students of SMA Negeri 3 Panyabungan. The instruments used in data collection are questionnaires and interviews. The results of the study showed that student activity during the online learning process at SMA Negeri 3 Panyabungan was less active. This was adjusted to the situation that occurred during the Covid-19 pandemic where learning was carried out by distance learning. During online learning, student learning activity could not be fully reached according to the indicators of student learning activity, namely: 1) Visual Activities, paying attention, reading during the learning process, 2) Oral Activities, interacting in the learning process, 3) Listening Activities, listening and actively discussing, 4) Writing Activities, collecting assignments and making summaries, 5) Drawing Activities, making summaries, 6) Motor Activities, conducting experiments, 7) Mental Activities, responding, 8) Emotional Activities, interest, joy during the learning process.

Biology education requires a learning strategy that can improve conceptual understanding while developing students' critical thinking skills. This study aims to analyze the influence of the Project-Based Learning (PjBL) learning model on the learning outcomes of class X students on biodiversity materials at SMA Negeri 1 Melonguane. The research uses a quasi-experimental method with a Pretest-Posttest Control Group Design, involving two classes, namely the X-A experimental class taught using the PjBL model with a mini ecosystem project, and the X-D control class. The control class is taught conventionally. The analysis results showed a significant increase in the posttest score of the experimental class with an average of 79.33 compared to the control class with an average of 73.39. These findings indicate that applying the PjBL model effectively creates active learning, increases student engagement, and develops problem-solving skills and creativity. The lower variability of scores in the experimental class showed that PjBL could accommodate differences in students' abilities. Project-based learning also arouses students' motivation and confidence, making Biology learning more contextual and meaningful. The implications of this study show that applying the PjBL model can be an alternative to Biology learning strategies to improve learning outcomes while supporting the development of 21st century skills. This research enriches the literature on Biology learning innovations. It opens opportunities for further studies related to the application of PjBL to other materials or long-term impact analysis on students' academic achievements.

Microscopic staining is an important technique in observing plant tissue to increase contrast and clarify cellular structures. One of the dyes commonly used in plant tissue studies is Safranin, a cationic base compound from the azo group that has a high affinity for cell components that produce negative impacts such as lignin and nucleic acids. This study aims to determine the effectiveness of Safranin as an alternative dye in microscopic observation of spinach leaf tissue (Amaranthus spp.). The practicum was carried out using the wet preparation method, using cross-sections of spinach leaves stained with Safranin solution, then observed under a light microscope. The results showed that Safranin was able to provide good red color contrast to cell walls, cell nuclei, and blood vessel tissue, so that structures such as epidermis, palisade tissue, sponges, and xylem and phloem could be clearly identified. However, obstacles arise in the regulation of concentration and duration of staining, where excess of both can cause excess and excessive structural accuracy. Therefore, adjustment of technical parameters is needed to obtain optimal results. These findings indicate that Safranin is effective as a microscopic dye and has the potential to be used in educational practicum activities, because it is economical, safe, and easy to apply. This study also applies the interdisciplinary application of chemistry and biology in science learning.

The low learning outcomes of students in Biology learning at SMA Negeri 1 Tutuyan is the background for the need for learning innovations that are able to increase active participation and conceptual understanding. This study aims to analyze the effectiveness of the Group Investigation learning model in improving student learning outcomes in Cell materials. The research used a pseudo-experiment method with a one group pretest-posttest design. The research sample amounted to 35 students from class XI Science who were selected purposefully. The instruments used were multiple-choice tests, pre-tests and post-tests of 25 questions for each test. The results showed an increase in the average score from 23.14 in the pre-test to 83.14 in the post-test. Inferential data analysis using the t-test showed a t-calculated value of 89.55, far above the t-table at a significance level of 5%. The category of student learning outcomes has also undergone significant changes, from a very low majority to a high and very high majority. The application of the Group Investigation model encourages students to be active in investigation, group discussions, and independent learning, which strengthens their collective and individual mastery of the material. These findings prove that the Group Investigation model is effective in improving learning outcomes, and can be implemented as a learning strategy in the Independent Learning Curriculum. This study contributes to the development of cooperative learning models in the context of Biology learning at the high school level, and can be replicated in other materials and levels to strengthen its effectiveness.

Conceptual understanding is an important aspect of learning biology because it reflects the extent to which students can internalize knowledge and relate it to real life. This study aims to analyse the level of understanding of the concept of biodiversity of students and identify supporting and inhibiting factors in the biology learning process. The research was conducted in class X of SMA Negeri 1 Pineleng with a descriptive qualitative approach. Data was collected through observation, written tests, questionnaires, teacher interviews, and documentation. Conceptual comprehension is measured using three main indicators: the ability to restate concepts, classify objects based on certain properties, and provide examples rather than definitions. The results showed that 87.5% of students had achieved a good level of understanding of classification indicators and giving examples, but only 50% were able to restate concepts completely. The supporting factors identified include learning motivation, parental mentoring, teacher learning strategies, and student absorption. On the other hand, barriers to understanding include lack of enthusiasm for learning, an unsupportive environment, and a lack of parental involvement. These findings confirm that the success of conceptual understanding is determined not only by cognitive aspects but also by comprehensive pedagogical and social support. This research makes an important contribution to the development of more contextual and participatory biology learning strategies, as well as opens opportunities for further research that explores the use of innovative media in strengthening conceptual understanding.

The low activity of students in biology learning is the main challenge in efforts to improve the quality of science learning in secondary schools. This study aims to analyze the effectiveness of the application of the Project Based Learning learning model in increasing student activity and learning outcomes in respiratory system materials at SMA Negeri 2 Langowan. This research is a Class Action Research that is carried out in two cycles, each of which includes stages of planning, action, observation, and reflection. The subjects of the study were 13 students in grade XI. The instruments used include student activity observation sheets and the final test of the cycle based on description questions. The results showed that in the first cycle, only 38.46% of students achieved learning completeness with an average score of 60. However, after improving the strategy in cycle II through active mentoring, learning motivation, and strengthening group work, completeness increased to 92.30% with an average score of 84.69. These findings indicate that the Project Based Learning learning model is able to build student activeness through contextual, collaborative, and hands-on experience-based learning. The increase in activeness is reflected not only in test results, but also in students' active participation in discussions, group presentations, and project completion. The implications of this study show that Project Based Learning is feasible to be implemented as an innovative learning strategy to improve the quality of student interaction and engagement in biology learning on an ongoing basis.

This study aims to examine the implementation of the independent learning curriculum in biology at SMA Negeri 1 Waigete. The objectives are: (1) to describe how the independent learning curriculum is applied in biology classes; (2) to identify the challenges teachers face during its implementation; and (3) to explore the strategies employed by teachers to overcome those challenges. Using a descriptive-qualitative approach, the research collects data through interviews, observations, and documentation. Interviews were conducted with the principal, vice principal of curriculum, and biology teachers. Observations were made during the learning process in the classroom, while documentation included teaching modules and supporting articles. Data analysis was carried out in three stages: data coding, presentation, and drawing conclusions. The findings indicate that the independent learning curriculum in biology for Class X (Phase E) was implemented effectively, with teachers preparing KOSP, learning tools, and providing materials such as readings and videos. Teaching also considered local potential and involved both formative and summative assessments. However, several problems were identified, including limited facilities and infrastructure, lack of internet access, insufficient training, and some students lacking personal devices. Moreover, teachers experienced difficulty in selecting appropriate learning strategies to engage students effectively. To address these issues, teachers have participated in curriculum-related training and prepared printed materials and pre-downloaded videos for students without digital access. These efforts show a proactive approach to ensuring all students can benefit from the independent learning curriculum despite existing limitations.

Improving students' Biology learning outcomes is a major challenge in the learning process in high school, especially when the material taught is abstract and complex, such as the human digestive system. This study aims to analyze the effectiveness of the Problem-Based learning model assisted by mind mapping in improving the learning outcomes and activities of grade XI students of SMA Negeri 2 Tondano. This research is a Classroom Action Research conducted in two cycles, each consisting of two meetings. Each cycle involves planning, action execution, observation, and reflection activities, with data collection through observation, testing, and documentation. The results of the study showed a significant increase in learning activities and student learning outcomes. The average learning activity increased from 54.6% in the first cycle to 81% in the second cycle, while the percentage of completeness of learning outcomes increased from 59% to 88%. This increase is influenced by the active involvement of students in group discussions, visualisation of concepts through mind mapping, and the role of teachers as facilitators in problem-based learning. These findings indicate that the integration of the Problem-Based model with mind mapping is effective in building critical thinking skills, improving concept understanding, and encouraging active student participation. The implications of this study show the importance of implementing innovative learning strategies to improve the quality of Biology learning in secondary schools.

Low motivation to learn and limited conceptual understanding are significant challenges in learning biology at the junior high school level, especially in abstract material on the classification of living things. This study aims to analyze the influence of the use of animated videos on student learning motivation and learning outcomes. A quantitative approach with a pre-experimental design of one group pre-test and post-test was used in this study. The research sample consisted of 25 students in grade VIII of SMP Negeri 2 East Likupang. Data was collected through observation sheets to assess learning motivation and pre-test and post-test tests to evaluate learning outcomes. The results of the observation showed that the average motivation of students reached 86.32% in the outstanding category. The average pre-test score of 59.57 increased to 81.09 on the post-test, indicating a significant improvement in concept comprehension. The t-test corroborates these findings with a significant value at a 95% confidence level. These results show that animated videos can present a visualization of complex concepts in an engaging and contextual way, thereby increasing students' focus, active participation, and absorption of the material. In addition, the use of this media also supports the development of students' collaborative skills and critical thinking. This research confirms that animated videos are a strategic alternative in effective science learning, especially in school environments with limitations of conventional visual media. It is suggested that the application of this media be expanded to various materials and levels of education.

  This study aims to analyze student activities and learning outcomes in the learning of Spermatophyta sub-material through practicum activities based on Exploring the Environment at SMP Negeri 4 Manado. The background of this study is based on the need for a contextual approach to biology learning and being able to increase student engagement actively. The method used was quantitative descriptive research with survey techniques, involving 20 grade VII students and 2 science teachers as respondents. The research instrument consisted of a closed questionnaire, an open interview, and an observation sheet. The questionnaire results showed that students responded very well to this learning method, with an average score of 83.4%. At the same time, teachers rated this approach as effective, with an average of 88%. Observations showed that students were active in practicum activities, enthusiastic in making observations, and involved in group discussions. The interview revealed that this environment-based learning strengthens students' understanding of biological concepts and increases scientific curiosity. The obstacles found include the accuracy of recording and the inequality of roles in group work. However, overall, exploring the Surroundings-based learning has been proven to positively impact student engagement and understanding, especially in connecting theory with real objects in the surrounding environment. The implications of this study emphasize the importance of applying contextual approaches in biology learning and suggest improving guidance strategies and recording of observations in the field for more optimal learning effectiveness.