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Sri Rahayu; Mirza Anindya Pangestika

Jurnal Pengabdian dan Pembangunan Lokal 2026 Lembaga Pengembangan Kinerja Dosen

Early Childhood Education (PAUD) or Kindergarten (TK) is an important level of education in building the foundations for children's overall development, covering aspects of religious and moral values, physical-motor skills, cognitive skills, language, social-emotional skills, and the arts. The development of PAUD/TK currently shows a positive trend, as seen in the increasing public awareness, number of institutions, and participation of children aged 4–6 years. However, challenges still arise, such as limited facilities and infrastructure, variations in educator competence, differences in access to services between regions, and the need for stimulation at home. Competition among early childhood education institutions is quite fierce, driving innovation in learning quality, teacher competence, facilities, and flagship programs. In this context, marketing management plays a strategic role in building the institution's image, conveying information about educational services, identifying community needs, and increasing the institution's competitiveness in an ethical and sustainable manner. TK Muslimat NU Pakembaran, as a religious-based PAUD institution, plays an important role in developing children's development and instilling Islamic values. Although the marketing strategy is still simple and predominantly based on a familial approach and word-of-mouth promotion, these efforts are effective in building community trust and loyalty. In the future, strengthening a more systematic marketing management can increase competitiveness, expand the reach of information, and support the sustainability of the institution in providing quality, religious, and character-building early childhood education services.

Velika Occalanie; Peter Peter; Henky Lisan Suwarno

International Journal of Economics, Commerce, and Management 2026 Asosiasi Riset Ekonomi dan Akuntansi Indonesia

Food and beverage companies must maintain a robust capital structure to compete effectively amidst the intense pressures of globalization and achieve their strategic objectives. This study aims to examine the impact of profitability, asset structure, company size, and solvency on the capital structure of food and beverage firms listed on the Indonesia Stock Exchange (IDX) and included in the LQ45 index. This study uses an explanatory method with purposive sampling technique, where samples are determined based on companies that have completed financial reports during the research period and are indexed in LQ45. Data analysis was performed using t-tests and F-tests. The results show that profitability (ROA), asset structure, and company size (Ln Total Assets) do not have a significant partial effect on capital structure (DER), meaning that these three factors do not directly influence companies' decisions on the use of debt for financing. However, solvency (DAR) was found to have a significant effect on capital structure, indicating that a company's ability to meet its long-term obligations plays an important role in determining the level of debt used for operational financing. Simultaneously, the four independent variables had a significant effect on capital structure, meaning that all variables together contributed to influencing food and beverage companies' decisions in determining their financing strategies.

Tiarnita Maria Sarjani Br Siregar; Tetty Aprilya Rezeki Simatupang; Rani Natalia Purba; Niko Alriadi Sinaga

International Journal of Education and Literature 2026 Lembaga Pengembangan Kinerja Dosen

English learning at the elementary school level plays an important role in developing students’ early language competence, particularly in listening skills as the foundation for other language abilities. However, many learning processes still rely on conventional teaching methods that do not fully accommodate the developmental characteristics of young learners, resulting in limited engagement and listening comprehension. Therefore, this study aims to analyze the role of storytelling in improving English listening skills among elementary school students and to identify effective implementation strategies in classroom learning. This research employs a library research method by reviewing and analyzing various relevant literature sources, including books, scientific journals, and previous research findings related to storytelling in English language learning. The analysis shows that storytelling contributes significantly to improving students’ listening comprehension, vocabulary acquisition, attention, and learning motivation. Through structured narratives, expressive delivery, and the support of visual media, storytelling creates a contextual and meaningful learning environment that helps students understand spoken English more effectively. The findings also indicate that the effectiveness of storytelling is closely related to the developmental characteristics of elementary school students who learn better through concrete, imaginative, and engaging experiences. Furthermore, the implementation of storytelling through systematic stages pre-listening, while-listening, and post-listening can enhance students participation and comprehension during the learning process. In conclusion, storytelling can be considered an effective instructional strategy for improving English listening skills among elementary school students while also supporting vocabulary development and creating an engaging learning atmosphere.

Afrizal Bagaskara; Ni Made Laksmi Devi Jayanti; I Komang Aditya Negara

Jurnal Ilmu Kesehatan dan Gizi 2026 Pusat Riset dan Inovasi Nasional

Regular physical activity is known to play an important role in maintaining overall health, including improving immune system function and mental health. Lack of physical activity remains a public health issue, making it important to understand the impact of physical activity on the immune system and mental health. This study aimed to review the effect of regular physical activity on the immune system and mental health using a literature review method. The literature sources were obtained from publications within the last five years using databases such as Google Scholar, PubMed, and ScienceDirect. The articles were then screened and selected based on inclusion and exclusion criteria, resulting in nine relevant articles for analysis. The selection results indicate that regular physical activity can increase the mobilization and activity of immune cells such as lymphocytes, neutrophils, and natural killer cells, and also modify the adaptive immune system through changes in T cells and B cells. In addition, physical activity also improves mental health through neurobiological mechanisms such as increased endorphin, dopamine, and serotonin levels, which can reduce stress, anxiety, and depression, as well as improve sleep quality and psychological well-being. Therefore, regular physical activity has a positive effect on the immune system and mental health and can be used as a promotive and preventive strategy to maintain overall health.

Savira, Anggey; Indri Rahmadini; Trisya Oktaviani Indah

Jurnal Ekonomi dan Keuangan 2026 Asosiasi Riset Ekonomi dan Akuntansi Indonesia

This study aims to analyze the factors that influence economic growth. Economic growth is an important indicator used to measure the success of a country's development because it reflects the increasing capacity of an economy to produce goods and services. This research uses a descriptive approach by examining several factors affecting economic growth, including investment, labor, unemployment, and international trade. The results show that investment plays a significant role in increasing production capacity and encouraging the development of industry and infrastructure. Labor also contributes to economic productivity through the production of goods and services. On the other hand, a high unemployment rate can hinder economic growth because part of the labor force is not absorbed into productive activities. In addition, international trade through export and import activities can support economic growth by expanding markets for domestic products and fulfilling the need for raw materials and technology in the production process. Therefore, economic development policies need to consider these factors to achieve sustainable economic growth and improve public welfare

Ketut Doni Trio Saputra; Didik Budiaynto; Muhajir Muhajir

Fauna: Jurnal Kajian Ilmu Hewani 2026 Asosiasi Riset Ilmu Tanaman Dan Hewani Indonesia

Vannamei shrimp (Litopenaeus vannamei) is one of the most important aquaculture commodities with high economic value and strong market demand. The transportation of broodstock shrimp is a critical stage that may affect survival due to decreased oxygen levels and water quality deterioration. High stocking density during transportation can accelerate oxygen depletion and increase metabolic waste, leading to higher mortality. This study aimed to determine the effect of different packing densities on the survival rate of broodstock shrimp during 12 hours of transportation. The experiment used four treatments with densities of 6, 8, 10, and 12 shrimp per bag, each with six replications. Observed parameters included temperature, dissolved oxygen (DO), and survival rate. The results showed that lower stocking density produced higher survival rates, with the best result obtained at 6 shrimp per bag. Water quality remained within acceptable limits, although oxygen levels decreased with increasing density. Therefore, proper stocking density is essential to maintain shrimp survival during transportation.  

Aprilinda M. Harahap; Syahrina Suhilah Siregar; Lolo Ate Karina Berutu; Lia Agita Sari; Andre Selamat Sinaga +2 more

Jurnal Ilmu Pendidikan, Politik dan Sosial Indonesia 2026 Asosiasi Peneliti dan Pengajar Ilmu Hukum Indonesia

This study aims to analyze the political strategies implemented by the Partai Keadilan Sejahtera faction in advocating community aspirations within the North Sumatra Provincial Regional House of Representatives. The research employs a qualitative approach using a descriptive method. Data were collected through interviews, observation, and documentation. Interviews were conducted with Hannah May Lisa as a PKS staff member and Riduansyah as a faction expert staff at the DPRD Secretariat of North Sumatra. The results indicate that the PKS faction’s political strategy in advocating public aspirations involves several stages: gathering community aspirations through recess activities and direct communication with constituents, internal faction discussions to determine priority issues, and political communication and collaboration with other factions and the regional government. In addition, the role of faction expert staff is essential in providing policy analysis and supporting data for the decision-making process. Therefore, faction political strategies play an important role in ensuring that public aspirations are accommodated in regional public policies.

Nia Plania Sitompul; Hari Ghanesia Istiani

DIAGNOSA: Jurnal Ilmu Kesehatan dan Keperawatan 2026 International Forum of Researchers and Lecturers

Fine motor skills in preschool children begin to develop, where they can use their fingers to write, draw, and so on. The developmental stages of each child are the same, namely the maturation of motor organs. At this age, it is seen as a very important phase because an individual experiences very rapid development. The purpose of this study was to determine the effect of a combination of finger painting and origami activities on the fine motor development of children aged 4-6 years at Cikal Depok Kindergarten in 2025. The research method used in this study was a Quasy experiment design with one group pre-test-posttest. Data analysis was obtained using a non-parametric test of marginal homogeneity with a p-value of 0.007 <0.05, meaning there is an effect of the combination of finger painting and origami activities on the fine motor development of children aged 4-6 years at Cikal Depok Kindergarten in 2025. The conclusion is that finger painting and origami can provide a stimulus for the motor development of children aged 4-6 years. Suggestion: Children's developmental stages can be stimulated with various other teaching aids, finger painting and origami are effective teaching aids.

Elsa Meylani; Udi Utomo

Jurnal Riset Rumpun Seni, Desain dan Media 2026 Pusat Riset dan Inovasi Nasional

This study aims to explain how to instill character values ​​through extracurricular drumband activities at SD N 2 Musuk, located in Boyolali Regency. The rationale behind this research is because extracurricular activities are important in shaping students' character in accordance with the institution's vision and mission. The method applied in this study is descriptive qualitative. As research subjects, there are 45 students from grades 4, 5, and 6, and involving the principal, teachers, and coaches as sources of information. Data collection was conducted through observation, interviews, questionnaires, and documentation, which were then analyzed descriptively through data reduction, information presentation, and drawing conclusions. The research findings indicate that character instillation is carried out through a well-planned program and regular, scheduled practice. The character values ​​that were successfully instilled include discipline, responsibility, hard work, independence, collaboration, as well as religious attitudes and tolerance. The results of this activity are increased self-confidence and social skills of students, as well as the achievement of non-academic achievements that are a source of pride for the school.

Penina Fakdawer; Indra Kertati; Charis Charistiani

International Journal of Communication, Tourism, and Social Economic Trends 2026 Asosiasi Penelitian dan Pengajar Ilmu Sosial Indonesia

UMKM Digital transformation in local governance is an important instrument in realizing the principles of good governance, especially in regions with complex geographical challenges. This study aims to analyze the effectiveness of e-government implementation at the district level in encouraging community participation in the local development planning process, using a case study of the Fof District Office, Tambrauw Regency. The methods used were descriptive qualitative through field observations, in-depth interviews with district officials and community leaders, and analysis of development documents. The data was analyzed through the stages of reduction, presentation, and drawing conclusions. The results of the study show that the implementation of e-government in Fef District is in a transition phase. Bureaucratic digitization improves administrative efficiency and transparency of public information. However, its effectiveness in expanding community participation is still low. The main obstacles include limited telecommunications infrastructure in several villages and low community digital literacy. As a result, participation in Musrenbang remains dominated by conventional mechanisms, while digital platforms serve primarily as a means of one-way reporting. This study emphasizes that the success of digital transformation depends on the readiness of infrastructure and community capacity, so a hybrid approach that integrates technology with local wisdom is needed to strengthen participation in regional development.

Aliya Rahmadani; Tanti Restiasih Skober

International Journal of Education and Literature 2026 Lembaga Pengembangan Kinerja Dosen

This research is entitled The Influence of Japanese Pop Culture on Children’s Toys Trends in Indonesia, 1980-2010. The study employs the historical method, which consists of four stages: heuristics, source criticism, interpretation, and historiography.John Fiske’s (2011) theory of popular culture and  Joseph Nye’s concept of soft power is used to analyze how Japanese popular culture, particularly through entertainment media and toy products, was disseminated persuasively and received by Indonesian children. Since the early 1990s, mass media such as television and children’s magazines have played an important role in introducing Japanese anime and characters, which subsequently encouraged the emergence of toy trends based on Japanese popular culture. The object of this research focuses on children’s toys influenced by Japanese popular culture that circulated in Indonesia between 1980 and 2010, including Tamiya, Beyblade, Tamagotchi, Crush Gear, B-Daman, and yo-yo. These toys not only developed as commercial products, but also contributed to the formation of children’s play patterns, communities, and social identities. However, during the period from 2008 to 2010, these toy trends began to decline due to the advancement of digital technology, changes in children’s consumption patterns, broadcast censorship policies implemented by Indonesian Broadcasting Commission (KPI), and increasing competition from popular culture of other countries such as Korea, the United States, and China. This condition reflects a shift in children’s play culture from physical toys to more modern forms of digital entertainment.

Septia Wahyuni; Delfia Herwanis; Al Musanna; Suminah Suminah; Rahmanita Zakaria +1 more

International Journal of Education and Literature 2026 Lembaga Pengembangan Kinerja Dosen

Curriculum reform in Indonesia has repeatedly emphasized flexibility, deep learning, and student-centered instruction. Nevertheless, many reforms have not been translated effectively into classroom practice. Existing studies tend to focus on teacher competence and policy readiness, while the deeper dimension of teacher beliefs remains underexplored. This study aims to unpack how teacher beliefs shape responses to curriculum reform and to identify the belief-related factors that influence the success or failure of curriculum innovation. A qualitative descriptive design was employed using pre-Delphi reflective questionnaires and Focus Group Discussion data involving senior secondary school teachers and educational stakeholders. Data were analyzed thematically to identify recurring patterns of belief, challenge, and adaptation. The findings reveal that teacher beliefs constitute the most decisive factor in curriculum implementation. Three major themes emerged: (1) a persistent gap between curriculum policy and classroom practice, (2) the difficulty of transforming teachers’ mindsets from fixed to growth-oriented perspectives, and (3) the construction of an ideal teacher identity characterized by reflection, adaptability, collaboration, and moral commitment. More importantly, teachers viewed curriculum change not merely as a technical demand, but as a moral and spiritual responsibility. Curriculum reform will remain ineffective unless it addresses teachers’ internal beliefs. Sustainable transformation requires professional development that integrates cognitive, emotional, and spiritual dimensions of teaching.      

Sri Sudharmi; Nila Widya Keswara

International Journal of Medicine and Health 2026 Lembaga Pengembangan Kinerja Dosen

The postpartum period is an important time for the mother's physiological recovery after childbirth, during which various bodily changes are still ongoing and health risks, including changes in blood pressure, may occur. One factor that is thought to affect blood pressure during the postpartum period is dietary patterns, particularly high sodium intake. Salted fish is a food commonly consumed by the community and is known to have a high salt content. This study aims to analyze the effect of salted fish consumption on blood pressure in postpartum mothers from day 1 to day 28. This study used a quantitative approach with an analytical observational design through a cross-sectional method. The study sample consisted of 20 postpartum mothers on days 1–28 who were selected using purposive sampling. The independent variable was salted fish consumption, while the dependent variable was the systolic blood pressure of postpartum mothers. Data were collected through interviews using a structured questionnaire and blood pressure measurements with a standard tensiometer. Data analysis was performed using univariate and bivariate methods. The Shapiro–Wilk normality test showed that the data were not normally distributed, so the analysis was continued with the Wilcoxon test. The results of the study show a significant difference between systolic blood pressure before and after exposure to salted fish consumption, indicating that consumption of foods high in salt affects changes in blood pressure in postpartum women. This study concludes that salted fish consumption has the potential to affect the blood pressure of postpartum women. Therefore, nutrition education related to limiting the intake of foods high in salt during the postpartum period needs to be improved as part of efforts to prevent postpartum hypertension.

Jholant Bringg Luck Amelia; Wilson Bangun; Sofiyan Sofiyan

Proceeding of the International Conference on Management, Entrepreneurship, and Business 2026 Asosiasi Riset Ilmu Manajemen Kewirausahaan dan Bisnis Indonesia

Digital revolution and the disruption of the global labor market have made public organizations, especially vocational training institutions, even more complicated. This study seeks to examine the impact of dynamic skills on institutional resilience, with innovative leadership serving as a mediating variable. The study employed a quantitative methodology, utilizing data collected from 180 employees at the Medan Vocational and Productivity Training Center (BBPVP). We used Structural Equation Modeling based on Partial Least Squares (SEM-PLS) to look at the data. The findings indicate that dynamic capabilities exert a positive and significant influence on institutional resilience, underscoring the necessity for an organization to identify, capitalize on opportunities, and adapt to change. Dynamic qualities also have a big effect on inventive leadership, which is very important for making organizational change happen. Innovative leadership demonstrably influences institutional resilience and serves as a partial mediator between dynamic capabilities and institutional resilience. This research offers a theoretical advancement by synthesizing institutional theory, dynamic capacities, and innovative leadership to elucidate public organizational resilience. In practical terms, the findings of this study have significant significance for enhancing the adaptive capacity of vocational training institutions in the context of digital disruption.

Andreas Afriyanto Moa; Fitriah Fitriah; Mariana Sada

Jurnal Riset Rumpun Matematika dan Ilmu Pengetahuan Alam 2026 Pusat riset dan Inovasi Nasional

This study aims to determine the extent of the role of the school environment in influencing the learning motivation of class X students in Muhammadiyah Maumere High School. The school environment is an external factor that plays an important role in supporting or inhibiting the learning process of students. The factors studied in this study include the physical condition of the school (such as classrooms, libraries, laboratories, and sports facilities), social aspects (support of teachers and peers), as well as psychological aspects (comfort, appreciation, and conducive learning atmosphere). The method used in this study is a qualitative descriptive method with data collection techniques through observation, interviews, and questionnaires to students and teachers. The result of the study shows that school facilities in Muhammadiyah Maumere High School still have many limitations, including inadequate classrooms, lack of learning technology tools such as projectors, small libraries with limited collection of books, and the unavailability of special space for arts and special sport facilities. However, despite these limitations, most students still show a high level of learning motivation. This is influenced by the support of teachers and peers, involvement in extracurricular activities, family support, and personal expectations of student achievement. This study concluded that the school environment indeed plays an important role in shaping student learning motivation, but social and psychological factors also have a big influence. Therefore, it is recommended that schools make improvements and develop physical facilities and strengthen positive social climate to support the student learning process optimally. 

Syadzna Malika Maimun; Miswati Furqani; Hafizatun Suardi; Nabila Aini; Shahira Yasmin +1 more

Jurnal Riset Rumpun Matematika dan Ilmu Pengetahuan Alam 2026 Pusat riset dan Inovasi Nasional

This study aims to analyze the potential of eupatorin found in Cat’s Whiskers (Orthosiphon stamineus) as an anticancer agent using in silico methods. Eupatorin was selected due to its promising biological activity reported in previous literature. The research employed compound structure data registered in PubChem, analyzed through PASS Online for pharmacological activity prediction, ProTox-II for toxicity evaluation, and pkCSM for ADMET parameters. The results indicated that eupatorin has a high probability of inhibiting cancer cell proliferation with significant pharmacological activity values. Toxicity predictions showed a safe profile with an LD50 supporting its potential therapeutic use. ADMET analysis demonstrated that eupatorin possesses good bioavailability, adequate absorption and distribution, and minimal metabolic interactions, supporting its efficacy as an anticancer compound. These findings suggest that eupatorin could be an important candidate for herbalbased drug development, particularly for cancer therapy, and provide opportunities for further research through in vitro and in vivo experiments to validate anticancer activity comprehensively.

Najwa Hasyifa; Elifia Dwi Utami; Rizky Afifah Alfi; Widia Azhari Saputri; Nabila Az Zahra +3 more

Jurnal Riset Rumpun Matematika dan Ilmu Pengetahuan Alam 2026 Pusat riset dan Inovasi Nasional

Eukaryotic microorganisms, particularly fungi and protists, play a vital role in ecosystems, as well as in microbiology studies and biology education. The complex structure of eukaryotic cells, such as a true nucleus and membrane-bound organelles, enables these two groups to perform diverse physiological functions and adapt to their environments. This study aims to synthesize the literature on the structure and function of eukaryotic cells in fungi and protists and their implications for biology learning. The method used is a literature review with a descriptive qualitative approach through analysis of relevant scientific sources. The results of this study indicate that understanding the structure of eukaryotic cells in fungi and protists is not only important in the context of microbiology but also contributes to strengthening understanding of the concept of cell structure and function and making biology learning more contextual and meaningful.

Putri Astri Rahmadani; Ibrahim Tohar; Rahman Hakim

Konstruksi: Publikasi Ilmu Teknik, Perencanaan Tata Ruang dan Teknik Sipil 2026 Asosiasi Riset Ilmu Teknik Indonesia

The Regional Public Library of Sidoarjo Regency plays an important role as a public facility in supporting the development of community literacy. Along with population growth and the advancement of the education sector, the library is required to provide spaces that are comfortable, safe, and inclusive for all users. However, existing conditions indicate various architectural problems that potentially hinder the optimal function of the library. This study aims to identify architectural issues at the Regional Public Library of Sidoarjo Regency, including building conditions, spatial layout, user comfort, and the availability of thematic literacy facilities and inclusive spaces. The research method employed observation and literature review. Field observation was conducted to document the actual conditions of the building and library facilities, while the literature review focused on library standards, building regulations, and relevant architectural theories. The results show that although the library building is structurally adequate, the quality of space has not fully met the required standards of comfort, safety, and functionality. The spatial arrangement remains conventional and lacks flexibility, limiting its ability to support diverse literacy activities. In addition, thematic literacy facilities and the application of inclusive design principles are still limited. This study concludes that architectural development efforts are needed, focusing on improving spatial quality, implementing adaptive spatial planning, and providing more diverse and inclusive literacy facilities. The findings are expected to serve as a reference for future development planning of the Regional Public Library of Sidoarjo Regency in order to enhance its role as an educational public space and a community literacy center.

Basheer Jameel

International Journal of Applied Mathematics and Computing 2026 Asosiasi Riset Ilmu Matematika dan Sains Indonesia

The Fréchet distribution is one of the commonly used Extreme Value Distributions (EVDs) in statistical modeling and heavy-tailed data analysis, where it plays an important role in describing product lifetimes as well as climatic and financial phenomena. The estimation of its two parameters, namely the shape parameter and the scale parameter, is traditionally based on the Maximum Likelihood Estimation (MLE) method. However, maximizing the likelihood function for this distribution involves numerical difficulties, which necessitates the use of numerical optimization methods. In this study, we propose the use of the Aquila Optimizer (AO), a recent metaheuristic algorithm inspired by the hunting behavior of eagles, as an efficient numerical tool for maximizing the likelihood function of the Fréchet distribution. The objective function was formulated as the negative log-likelihood function (-LogL), and the Aquila Optimizer was employed to obtain the optimal estimates of the distribution parameters. Several simulation experiments with different sample sizes were conducted to compare the performance of the proposed method with a conventional approach represented by the Nelder–Mead method, using the Mean Squared Error (MSE) criterion. The simulation results demonstrated that the Aquila Optimizer outperformed the Nelder–Mead algorithm in many cases, although the superiority was slight. The results also showed that both algorithms were consistent, as their MSE values decreased with increasing sample size. In addition, a practical application was carried out using real data, and the results of the survival function estimation indicated a good fit.

Dian Lestari; Nurul Hayati; Muhajir Muhajir

Fauna: Jurnal Kajian Ilmu Hewani 2026 Asosiasi Riset Ilmu Tanaman Dan Hewani Indonesia

Based on data from FAO (Food and Agriculture Organization of The United Nations), it has been predicted that the aquaculture subsector will become one of the most relied-upon resources in the future, especially in meeting food needs, fish-based nutritional needs, and empowering the global community's economy (FAO, 2016). Catfish is a type of freshwater fish that can grow and develop well because it can be cultivated in limited land areas (Marsela, 2018). The frequency of feeding is one of the important factors that need to be considered in catfish farming. To determine the effect of different feeding frequencies on the absolute weight of dumbo catfish, a study was conducted using an experimental design called the Completely Randomized Design (CRD), with 3 treatments and a total of 8 replications. The test treatments were as follows: Treatment 1 (feeding twice a day), Treatment 2 (feeding three times a day), and Treatment 3 (feeding four times a day). The results of the ANOVA test showed a significant value of 0.000 (P < 0.05), indicating a significant difference in each of the different feeding frequencies. The analysis of the Least Significant Difference (LSD) test at a 5% significance level also showed that the relationship between each treatment had a significant value of 0.000 (P < 0.05). The average absolute weight of the fish for Treatment 1 was 12.99 grams, for Treatment 2 was 15.03 grams, and for Treatment 3 was 17.03 grams. The highest growth in the absolute weight of dumbo catfish fry occurred in Treatment 3 (feeding four times a day).