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This study aims to investigate the effects of tamarind leaf extract (Tamarindus indica L.) on neuropharmacological activity in mice using calculated percentages of responses based on the parameters PSM, SSSP, DSSP, SL, RO, SM, PSL, and ANA. This research was conducted as a laboratory experiment using a completely randomized design (CRD) with three treatment concentrations: 1%, 2%, and 4%. Observations were performed to assess the percentage of activity produced by each sample concentration, followed by descriptive–quantitative analysis to determine the dose–response pattern. The results showed that tamarind leaf extract produced varying responses across concentrations. The SSSP, ANA, and RO effects demonstrated increased activity at the 2% concentration, whereas other parameters (PSM, PSL, SM, SL, and DSSP) showed decreased activity. Interestingly, the SSSP parameter exhibited a positive dose–response pattern with the highest activity of 55.84% at 2%. Overall, the effectiveness of tamarind leaf extract depends on the concentration level. The 2% concentration appears to be the optimal dose for several neuropharmacological effects, while the 4% concentration was most effective only for SSSP. These findings highlight the importance of multi-concentration testing to determine effective dosing of natural products for biological applications and the need for further investigation.

The puring plant (Codiaeum variegatum), which has been known as an ornamental plant, apparently has significant therapeutic potential, supported by the presence of secondary metabolites such as flavonoids, tannins, saponins and alkaloids. Various modern studies report the biological activity of croton leaves, including anti-cholesterol, anti-hyperglycemia, antibacterial effects, and their use in biosurfactant formulations. However, studies on how varying extract concentrations influence bioactivity parameters quantitatively are still limited, especially in the context of dose-response relationships involving many biological parameters in one series of research. Therefore, this research was conducted to evaluate eight bioactivity parameters, namely PSM, SSSP, DSSP, SL, RO, SM, PSL, and ANA at croton leaf extract concentrations of 1%, 2%, and 4%. This research uses a laboratory experimental approach by extracting croton leaves through a maceration method using ethanol, then formulated in three concentration levels. Each parameter was analyzed quantitatively to observe the pattern of changes in biological response to variations in the dose administered. The results showed that most of the PSM, SSSP, DSSP, SL, and PSL parameters showed an optimum response at a concentration of 2%, which indicates that there is a most effective dose point before a decrease in activity occurs at the highest concentration. In contrast, several parameters such as RO, SM, and ANA showed a more fluctuating response, even the ANA parameter gave the highest value at a concentration of 4%, indicating that certain activities require higher compound levels to be achieved. In general, the response pattern found was not linear, but instead formed an optimum curve typical of the bioactivity of plant extracts. These findings confirm that determining dosage is very important in the use of croton leaf extract, both for pharmacological purposes and for the formulation of natural ingredient-based products.

Indonesia is recognized as one of the world’s richest countries in terms of biodiversity, including a wide variety of traditional medicinal plants. One of the lesser-known yet promising local species is matoa (Pometia pinnata J.R. Forst & G. Forst), whose leaves have been traditionally used by local communities to treat various ailments, including diarrhea and seizures. This study aims to investigate the pharmacological effects of matoa leaf extract, with a particular focus on its anticonvulsant activity, while also reviewing its potential chemical constituents as a basis for herbal medicine development. The research was conducted using a combination of literature review and experimental testing on mice (Mus musculus) induced with strychnine to trigger seizures. Key observed parameters included seizure onset time and survival duration (dead time) following the administration of either synthetic drugs or matoa leaf extract. The results demonstrated that the matoa leaf extract exhibited significant anticonvulsant effects, as indicated by prolonged seizure onset and increased survival time in test animals. These pharmacological effects are presumed to be related to the presence of active compounds such as flavonoids, tannins, and alkaloids, which may contribute to the stabilization of the central nervous system. Compared to conventional synthetic anticonvulsants, matoa leaf extract may offer a safer, plant-based alternative with potentially fewer side effects. The findings of this study provide valuable scientific evidence supporting the potential of Pometia pinnata leaves in the development of Indonesian herbal phytopharmaceuticals. Furthermore, they highlight the importance of further research, including preclinical and clinical trials, to validate efficacy, determine optimal dosages, and ensure safety for human use.

Pain is an unpleasant sensation that can interfere with an individual's quality of life and requires effective management. Synthetic analgesics such as ketorolac, codeine, etoricoxib, and celecoxib are commonly used to relieve pain, but long-term use can cause adverse side effects, including gastrointestinal disturbances, dependence, and cardiovascular risks. Therefore, it is important to find safer, natural alternatives. One promising alternative is the extract of the dragon’s tail leaf (Rhaphidophora pinnata), a plant traditionally used in Indonesian medicine as a pain reliever. This study aims to evaluate and compare the analgesic effects of Rhaphidophora pinnata leaf extract with several synthetic drugs using the writhing test on male mice (Mus musculus). The mice were induced with pain by injecting a 0.5% acetic acid solution. The treatments included ketorolac, codeine, etoricoxib, celecoxib, and Rhaphidophora pinnata leaf extract. The results showed that all treatments, including both synthetic drugs and Rhaphidophora pinnata leaf extract, produced significant analgesic effects compared to the negative control (Na CMC 0.5%). Ketorolac showed the highest effectiveness, followed by codeine, etoricoxib, and celecoxib. Although the Rhaphidophora pinnata leaf extract showed potential as a natural analgesic, its effectiveness varied among individual test animals. Some mice showed a better response to the leaf extract, but overall, the analgesic effect was still lower than that of synthetic drugs. These findings support the use of medicinal plants as a safer alternative to synthetic analgesic drugs. Moreover, this study provides a foundation for further research aimed at isolating active compounds from Rhaphidophora pinnata leaf extract to develop more effective and safer pain-relieving medications.

Pharmacology is the study of drug interactions with living organisms. This study aims to evaluate the anti-inflammatory effects of several drug preparations, including Hemilon (methylprednisolone), Lupred (prednisolone), Kamathasone (dexamethasone), neem leaf infusion, and snakehead fish extract, on mice (Mus musculus) induced with inflammation using carrageenan solution via intraplantar injection. Edema volume was observed using a plethysmometer at 15, 30, 45, and 60 minutes post-induction. Data were analyzed using normality, homogeneity, ANOVA, and Tukey tests via SPSS. Results showed that Hemilon had the highest anti-inflammatory effect, followed by Kamathasone and Lupred, while neem leaf and fish extract showed weaker activity. Statistical tests revealed normal distribution (sig. 0.197 > 0.05), non-homogeneous data (sig. 0.022 < 0.05), and no significant difference between groups (ANOVA sig. 0.366 > 0.05). Despite this, descriptive results confirmed strong anti-inflammatory potential of synthetic drugs. This practicum provides valuable insights into drug mechanisms and emphasizes methodological accuracy in pharmacological testing.

This study explores the use of psychotropic substances, which affect the central nervous system and can alter mental functions, behavior, and individual perception. In the medical context, psychotropics have been widely utilized for treating mental disorders such as depression, anxiety, and schizophrenia. The research emphasizes the importance of understanding the mechanisms of psychotropics to optimize their therapeutic benefits while minimizing the risk of adverse effects. The study employed animal models, specifically rats, using the forced swimming test (FST) to assess the antidepressant and sedative effects of various substances, including Esilgan, Xanax, Zolmia, Syzygium malaccense leaf extract, and lelap (herbal), with NaCMC as a negative control. The results demonstrated varying sedative and antidepressant effects among the substances, with Xanax exhibiting the most significant antidepressant effect, while Syzygium malaccense leaf extract showed potential as a natural sedative agent. The study also underscores the importance of applying the 3R ethical principles (Replacement, Reduction, Refinement) in animal research and highlights the need for alternative methods to reduce reliance on animal testing. These findings are expected to serve as a foundation for developing safer, more effective, and ethical psychotropic therapies for mental disorders in the future.

Fever is a condition characterized by an increase in body temperature as a response to infection or inflammation, and it is one of the most common clinical symptoms in various diseases. The excessive use of synthetic antipyretics such as paracetamol can lead to side effects; therefore, safer and natural-based alternatives are needed. This study aims to determine the antipyretic activity of betel leaf and to compare its effectiveness with that of paracetamol.This research was conducted using an experimental laboratory method with a completely randomized design. The test subjects were male white rats (Rattus norvegicus) of the Wistar strain, induced with fever using a 20% yeast suspension. The rats were divided into five groups: negative control, positive control (paracetamol), and three treatment groups with low, medium, and high doses of the extract. Body temperature was measured every 30 minutes for 180 minutes after treatment. The data were statistically analyzed using one-way ANOVA followed by Tukey's post hoc test.The results showed that the [plant extract] exhibited significant antipyretic activity (p < 0.05) compared to the negative control, especially at medium and high doses. The effectiveness of the highest dose was comparable to that of paracetamol. This antipyretic effect is presumed to be associated with the presence of flavonoids and tannins that may inhibit the synthesis of prostaglandin E2 (PGE2), a key mediator in the fever response.In conclusion,betel leaf has potential as an effective natural antipyretic agent and may be developed as a safer herbal-based alternative for fever treatment.

Indonesia, considered the country with the second-highest level of biodiversity in the world after Brazil, possesses extraordinary biological wealth that remains underutilized. The field of traditional medicine utilizes natural resources to prevent and treat physical and mental disorders. Periwinkle, or Catharanthus roseus, is one of the most widely used plants due to its abundance of bioactive phytochemicals, such as alkaloids, flavonoids, saponins, and tannins. The ethanol extract from Periwinkle leaves has the potential to act as a natural antipyretic agent that can reduce body temperature during fever. The purpose of this study is to review and analyze previous research findings on the antipyretic effects of ethanol extract from Periwinkle leaves on mice (Mus musculus). According to a literature review from various sources, the ethanol extract of Periwinkle leaves demonstrates a comparable level of effectiveness to synthetic antipyretic drugs in lowering the body temperature of fever-induced mice. Furthermore, the content of active compounds and the antipyretic performance of this plant are influenced by its variety and environmental factors. Body temperature begins to decrease one hour after extract administration and reaches its peak reduction at the third hour. Therefore, Periwinkle may serve as an alternative natural antipyretic remedy. However, further research is necessary to standardize its usage and conduct clinical trials on humans.

Diabetes mellitus is one of the chronic diseases whose prevalence is increasing worldwide. This disease is characterized by high blood glucose levels due to impaired insulin production or function, the hormone that regulates blood sugar levels. This study aims to examine the effect of wild betel leaf extract (Piper aduncum) on the inhibition of glucose transport using the ileum of white rats (Rattus norvegicus) with the infusion method. This research is a pre-experimental study with a posttest-only control design using white rats and wild betel leaves. The study demonstrates that wild betel leaves (Piper aduncum L.) and red betel leaves (Piper crocatum) have significant potential in inhibiting glucose transport and reducing blood sugar levels. This is evident from the significant decrease in the rate of glucose transport after the administration of wild betel leaf extract, indicating an inhibitory effect on glucose transporters such as SGLT-1 and GLUT-2.