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This study aims to comparatively analyze the effects of two types of inhalation anesthetics, ether and chloroform, on Wistar rats, focusing on three main parameters: induction time, duration of anesthesia, and side effects. Selecting the appropriate anesthetic method is crucial in animal studies to ensure animal welfare and the validity of the results. Twenty male Wistar rats, with an average body weight of 200–250 grams and 10–12 weeks of age, were randomly divided into two treatment groups of 10 rats each. The first group was anesthetized using ether, while the second group was anesthetized using chloroform, with doses adjusted to achieve adequate levels of anesthesia. Measurements were made of the induction time, which is the interval from the start of anesthetic exposure until the loss of the pedal reflex; the duration of anesthesia, which is the length of time until the rat regains consciousness; and clinical observations of any side effects that appear, both during and after anesthesia. The results showed that the ether group had a relatively longer induction time than the chloroform group. However, ether provided a longer and more stable duration of anesthesia, with a better recovery rate. In contrast, chloroform produced a shorter induction time but was accompanied by a shorter duration of anesthesia and a higher risk of side effects, particularly liver dysfunction and respiratory depression. These findings reinforce previous reports that chloroform has significant potential toxicity, and its use in animal research should be limited. Based on these results, ether is recommended as a relatively safe inhalation anesthetic option for laboratory procedures in Wistar rats, although its use still requires strict supervision and appropriate handling procedures to ensure the safety of both researchers and experimental animals.

Pain is an unpleasant sensation that can interfere with an individual's quality of life and requires effective management. Synthetic analgesics such as ketorolac, codeine, etoricoxib, and celecoxib are commonly used to relieve pain, but long-term use can cause adverse side effects, including gastrointestinal disturbances, dependence, and cardiovascular risks. Therefore, it is important to find safer, natural alternatives. One promising alternative is the extract of the dragon’s tail leaf (Rhaphidophora pinnata), a plant traditionally used in Indonesian medicine as a pain reliever. This study aims to evaluate and compare the analgesic effects of Rhaphidophora pinnata leaf extract with several synthetic drugs using the writhing test on male mice (Mus musculus). The mice were induced with pain by injecting a 0.5% acetic acid solution. The treatments included ketorolac, codeine, etoricoxib, celecoxib, and Rhaphidophora pinnata leaf extract. The results showed that all treatments, including both synthetic drugs and Rhaphidophora pinnata leaf extract, produced significant analgesic effects compared to the negative control (Na CMC 0.5%). Ketorolac showed the highest effectiveness, followed by codeine, etoricoxib, and celecoxib. Although the Rhaphidophora pinnata leaf extract showed potential as a natural analgesic, its effectiveness varied among individual test animals. Some mice showed a better response to the leaf extract, but overall, the analgesic effect was still lower than that of synthetic drugs. These findings support the use of medicinal plants as a safer alternative to synthetic analgesic drugs. Moreover, this study provides a foundation for further research aimed at isolating active compounds from Rhaphidophora pinnata leaf extract to develop more effective and safer pain-relieving medications.

This study explores the use of psychotropic substances, which affect the central nervous system and can alter mental functions, behavior, and individual perception. In the medical context, psychotropics have been widely utilized for treating mental disorders such as depression, anxiety, and schizophrenia. The research emphasizes the importance of understanding the mechanisms of psychotropics to optimize their therapeutic benefits while minimizing the risk of adverse effects. The study employed animal models, specifically rats, using the forced swimming test (FST) to assess the antidepressant and sedative effects of various substances, including Esilgan, Xanax, Zolmia, Syzygium malaccense leaf extract, and lelap (herbal), with NaCMC as a negative control. The results demonstrated varying sedative and antidepressant effects among the substances, with Xanax exhibiting the most significant antidepressant effect, while Syzygium malaccense leaf extract showed potential as a natural sedative agent. The study also underscores the importance of applying the 3R ethical principles (Replacement, Reduction, Refinement) in animal research and highlights the need for alternative methods to reduce reliance on animal testing. These findings are expected to serve as a foundation for developing safer, more effective, and ethical psychotropic therapies for mental disorders in the future.