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This study investigated the phytochemical screening and antibacterial activity against various microorganisms including E. coli, Staphylococcus aureus, Klebsiella pneumoniae, Enterococcus faecalis, and Candida albicans. Active compounds were isolated and identified from Aleppo Oak Gallnut, obtained from the Iraqi plant Quercus Infectoria L. Plant extracts were prepared using a continuous extraction apparatus, Soxhlet, with a successive solvent system based on polarity differences, including petroleum ether (60-80°C), ethanol (78°C), and hot aqueous extracts. Acid hydrolysis was performed on the raw ethanol and hot water extracts to obtain free phenolic compounds, including Gallic acid, Apigenin, Rutin, Kaempferol, Chlorogenic acid, and Caffeic acid, using high-performance liquid chromatographic (HPLC) techniques. The inhibitory activity of Aleppo Oak Gallnut extracts (Ethanol and Hot Aqueous) after acid hydrolysis was tested using four concentrations (25%, 50%, 75%, 100%) against various microorganisms. The Ethanol extract exhibited a significant inhibitory effect on Enterococcus faecalis. The Hot Aqueous extract showed a modest inhibitory effect on Enterococcus faecalis. The Ethanol extract demonstrated strong inhibition against Klebsiella pneumoniae. The Hot Aqueous extract at 100% and 75% showed high inhibition. The Ethanol extract exhibited significant inhibitory effects against Staphylococcus aureus at 100%. The Hot Aqueous extract at 75% and 100%  demonstrated weaker inhibition against Staphylococcus aureus. The Ethanol extract demonstrated weaker inhibition against E. coli, and the Hot Aqueous extract showed no effect at 50%  and 25%, and slight inhibition at 100%  and 75%. For Candida albicans, the Ethanol extract showed minimal inhibition at all concentrations and no effect at 25%. The Aqueous extract had a slight effect at 100%  and no inhibition at 75%, 50%, and 25%.

The use of antibiotics can cause drug resistance if their use is not appropriate, so alternative treatment from plants is needed, one of which is soursop leaves (Annona muricata L.). Chemical substances contained in soursop leaves can prevent development until they can kill bacteria, one of which is flavonoids. The purpose of this study was to determine whether soursop leaf methanol extract (Annona muricata L.) at concentrations of 25%, 50%, 75% can inhibit the growth of Staphylococcus aureus bacteria and can determine at what concentration soursop leaf extract (Annona muricata L.) can effectively inhibit Staphylococcus aureus. The simplisia extract was done by maceration strategy using methanol as a solvent. The antibacterial testing method uses the disc diffusion method and uses clindamycin as a positive control and distilled water as a negative control. The results showed that Annona muricata L. extract can provide antibacterial action at concentration variants of 25%, 50% and 75% which is characterized by the presence of transparent areas with an average diameter of 8.97mm, 11.36 mm and 13.3mm respectively with positive control with a diameter of 23.25 and negative control with a diameter of 0 mm. Soursop leaf methanol extract (Annona muricata L.) is able to suppress the growth of Staphylococcus aureus bacteria with the most effective concentration variant of 75%.    

Pumpkin (Cucurbita moschata) is an important vegetable because of its nutritional value and health benefits. This plant is a rich source of carotenoids, which are rich in water-soluble vitamins, phenolics, flavonoid polysaccharides, mineral salts, and vitamins, all of which are beneficial for health. Pumpkin fruit has a very thick and hard skin, so it can act as a barrier to the rate of respiration, the release of water through the evaporation process, and the entry of air that causes the oxidation process. The purpose of this study was to determine the antibacterial activity of pumpkin seed extract (curcubita moschata). ) against the inhibition of staphylococcus aureus bacteria by well diffusion method. This type of research is a true experimental laboratory using the well diffusion method. The ethanol extract of pumpkin seeds (curcubita moschata) which was tested using the well method was then divided into several concentrations including 10%, 15%, 20%, and 25%. On the NA media that has been made then smeared with Staphylococcus aureus bacteria which had been previously sterilized, then four wells were made in a petri dish after which pumpkin seed extract was added to each concentration. Chloramphenicol was used as a positive control as a positive control. It was then incubated at 37o for 24 hours, then the inhibition zone formed was then measured. The result of this research is pumpkin seed extract (curcubita moschata) has antibacterial activity in the presence of inhibition around the wells of each extract concentration. At a concentration of 10% extract with an inhibitory diameter of 19 mm, a concentration of 15% in an inhibitory diameter of 20 mm, a concentration of 20% in an inhibitory diameter of 21 mm and for a concentration of 15% with an inhibitory diameter of 24 mm, it can be concluded that the extract of pumpkin seeds (curcubita) moschata.) has antibacterial activity against Staphylococcus aureus.

Rumput laut atau alga (euceuma cottanii) telah dimanfaatkan penduduk pantai di Indonesia untuk bahan pangan dan obat-obatan. Negara kepulauan, Indonesia dapat memanfaatkan sumber daya perairan dengan maksimal untuk di jadi suatu produk farmasi. ,mengandung senyawa biokimia sebagai metabolik sekunder salah satunya sebagai aktivitas antibakti, Penilitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak etanol pada Eucheuma Cottonii dapat menghambat bakteri Staphylococcus Aureus. Metode yang di gunakan yaitu difusi sumuran dan metode in nova. Pada penelitian ini dilakukan uji skrining fitokimia dan uji aktivitas antibakteri ekstrak rumput laut hijau (euceuma cottanii) dengan menggunakan pelarut etanol 70%. Ekstraksi rumput laut hijau (euceuma cottanii) dilakukan dengan menggunakan metode maserasi untuk mendapatkan ekstrak. Hasil uji skrining fitokimia ekstrak etanol 70% rumput laut hijau (euceuma cottanii) memiliki kandungan metabolit sekunder seperti : flavonoid dan steroid. Ekstrak rumput laut hijau (euceuma cottanii) mempunyai kemampuan menghambat pertumbuhan bakteri staphylococus aureus dengan diameter zona hambat terbesar yaitu pada kosentrasi 30% sebesar 19 mm, dan diameter yang terkecil pada kosentrasi 10% sebesar 15 mm. Maka dapat di Simpulkan bahwa Hasil uji skrining fitokimia ekstrak etanol 70% rumput laut hijau (euceuma cottanii) memiliki kandungan metabolit sekunder seperti : flavonoid dan steroid. Ekstrak rumput laut hijau (euceuma cottanii) mempunyai kemampuan menghambat pertumbuhan bakteri staphylococus aureus dengan diameter zona hambat terbesar yaitu pada kosentrasi 30% sebesar 19 mm, dan diameter yang terkecil pada kosentrasi 10% sebesar 15 mm.

Tanaman obat merupakan tanaman yang sangat popular yang dapat dimanfaatkan sebagai bahan baku obat tradisional dan jamu, yang bila dikonsumsi akan meningkatkan kekebalan tubuh (immune system). Salah satu jenis tanaman yang berkhasiat obat adalah tanaman pepaya, mulai dari daun, buah hingga bijinya. Salah satu bagian dari pepaya yang ternyata juga memiliki khasiat sebagai bahan obat adalah kulit dari buah pepaya. Tujuan penelitian ini adalah untuk mengetahui kandungan senyawa metabolit sekunder yang ada di dalam kulit buah pepaya (carica papaya L.) Serta mengetahui aktivitas antibakteri ekstral etanol kulit buah pepaya (carica papaya L.) Terhadap bakteri Staphylococcus aureus. Ekstraksi dilakukan dengan menggunakan metode maserasi selama 3 hari dan remaserasi selama 1 hari dengan pelarut etanol 96%. Jenis penelitian ini adalah eksperimental laboratorium. Metode difusi agar sumuran digunakan untuk mengetahui aktivitas antibakteri ekstrak etanol kulit buah pepaya (carica papaya L.) Larutan dibuat dengan variasi konsentrasi yaitu 50% 100% dan 150% dalam b/v dengan kontrol positif kloramfenikol dan kontrol negatif aquadest. Pengamatan yang dilakukan untuk uji skrining fitokimia ekstrak etanol kulit buah pepaya asal Desa Negeri Lima mengandung senyawa tanin, alkaloid dan saponin. Hasil uji antibakteri ekstrak etanol kulit buah pepaya memiliki aktivitas antibakteri terhadap Staphylococcus aureus pada konsentrasi 50% sampai dengan 150% dengan rata-rata diameter zona hambat 16,00 mm sampai dengan 21,50 mm dengan kategori kuat hingga sangat kuat. Hal ini menunjukan bahwa ekstrak kulit buah pepaya (carica papaya L.) Mempunyai pengaruh terhadap pertumbuhan bakteri Staphylococcus aureus.