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Consumption of ethylene glycol–containing drugs can cause liver damage. This study aimed to evaluate the hepatoprotective potential of Moringa leaf extract (Moringa oleifera L.) on AST and ALT levels, liver morphology, hepatosomatic index, and liver histology in ethylene glycol–induced white rats (Rattus norvegicus L.). A Completely Randomized Design (CRD) was used with 20 male rats divided into five groups: normal control, ethylene glycol control, and three treatment groups (150, 300, and 450 mg/kg BW). Ethylene glycol was administered for 30 days, while the extract was given for 20 days. Blood samples were collected on day 31. Data were analyzed using One Way ANOVA and Duncan’s test. The results showed significant hepatoprotective effects (P = 0.000). AST and ALT levels in the treatment groups differed significantly from the normal control. Liver morphological changes were observed in both control and treatment groups. The hepatosomatic index, number of normal hepatocytes, and central vein diameter also showed significant differences. In conclusion, Moringa leaf extract demonstrates hepatoprotective potential by reducing AST and ALT levels, improving liver morphology, increasing normal hepatocytes, and decreasing central vein diameter, with the optimal dose at 450 mg/kg BW

Tomato (Solanum lycopersicum) extract is known to contain potent antioxidant compounds such as lycopene, vitamin C, and flavonoids that can protect reproductive cells from oxidative stress. This study aims to analyze the effectiveness of tomato extract in improving the quality of spermatozoa in white rats (Rattus norvegicus) exposed to cigarette smoke, which is a major source of free radicals. The research applied a literature review approach by identifying, selecting, and analyzing related studies published between 2013 and 2023 from the Google Scholar and Academia.edu databases. Ten eligible studies were included for synthesis. The results show that the administration of tomato extract at doses ranging from 20 to 60 mg/kgBW/day significantly increased sperm motility, morphology, and viability in rats exposed to cigarette smoke. The improvement was associated with the antioxidant effect of lycopene, which protects Leydig cells and maintains testosterone production. These findings suggest that tomato extract has significant potential as a natural antioxidant therapy to prevent oxidative damage and reproductive disorders caused by cigarette smoke exposure.

Elevated blood cholesterol levels are known to be a major risk factor for cardiovascular disease. Hypercholesterolemia can trigger atherosclerosis, which in turn increases the risk of coronary heart disease and stroke. One approach to managing cholesterol levels is through the use of natural ingredients with antihyperlipidemic activity. White turmeric (Curcuma zedoaria Rosc) is an herbal plant long used in traditional medicine and contains bioactive compounds such as curcuminoids and essential oils. These compounds are reported to have antioxidant and anti-inflammatory activities, as well as potential as agents for lowering blood lipid levels. Herbal-based therapies are increasingly considered as safe alternatives to synthetic drugs in managing hyperlipidemia. This study aims to evaluate the effectiveness of a microencapsulated preparation of white turmeric ethanol extract in reducing total cholesterol levels in male rats (Rattus norvegicus) induced by a high-fat diet. The study was conducted experimentally using 25 rats divided into six treatment groups. The groups consisted of normal controls, negative controls, positive controls using simvastatin, and three treatment groups given white turmeric extract microcapsules at concentrations of 0.25%, 0.50%, and 0.75%. The microencapsulation technique was applied to improve the stability and bioavailability of the turmeric extract. Cholesterol levels were measured before and after treatment. The results showed that the treatment group with the 0.25% concentration produced the most significant cholesterol reduction, from 110 mg/dL to 59 mg/dL. These findings indicate that even at a relatively low concentration, microencapsulated turmeric extract can significantly reduce cholesterol levels in vivo. Therefore, it can be concluded that white turmeric extract in microcapsule form is effective in lowering total cholesterol levels, especially at the 0.25% dose. This suggests that Curcuma zedoaria microcapsules hold promise as a natural therapeutic option for managing hypercholesterolemia and reducing cardiovascular risk.  

Dyslipidemia is a health problem characterized by impaired fat metabolism in the body, characterized by changes in blood lipid levels, either increasing or decreasing. This condition is generally seen through increased levels of total cholesterol, low-density lipoprotein (LDL), triglycerides, and decreased levels of high-density lipoprotein (HDL). This imbalance in the lipid profile, if persisted over the long term, has the potential to accelerate the formation of atherosclerotic plaque on artery walls, which can lead to cardiovascular disease. This study aims to determine the effectiveness of papaya leaf (Carica papaya L.) ethanol extract microcapsules in reducing cholesterol levels in white rats induced by a high-fat diet. The high-fat diet is formulated from a mixture of beef fat, eggs, and lard to increase cholesterol levels in a controlled manner. After the induction phase, the rats were divided into six treatment groups: a negative control group, a positive control group (simvastatin 0.36 mg), and three treatment groups with doses of papaya leaf ethanol extract microcapsules of 0.25%, 0.50%, and 0.75%. The treatment was given orally for 14 consecutive days. Total cholesterol levels were measured before and after treatment to evaluate the effectiveness of the intervention. The results showed that administration of papaya leaf extract microcapsules significantly reduced cholesterol levels. The 0.50% dose provided the most optimal effect, with a reduction reaching 77 mg/dL, greater than the simvastatin group. However, increasing the dose to 0.75% did not show a significant additional reduction effect, indicating an optimal dose-response point. Therefore, the 0.50% concentration can be considered the most effective dose and has the potential to be developed as an alternative natural cholesterol-lowering therapy. These findings also strengthen the potential use of papaya leaves as a bioactive source to support efforts to prevent and control dyslipidemia.  

Dyslipidemia is a lipid metabolism disorder characterized by elevated levels of total cholesterol, triglycerides, and low-density lipoprotein (LDL) accompanied by decreased levels of high-density lipoprotein (HDL). This condition is a major risk factor for atherosclerosis, coronary heart disease, and stroke. One plant with potential as an antidyslipidemic agent is bay leaves (Syzygium polyanthum). These leaves are known to contain active compounds such as flavonoids, saponins, tannins, alkaloids, and polyphenols that have antioxidant and hypolipidemic activities. The potential of natural compounds from medicinal plants to reduce blood cholesterol levels has gained significant attention in recent years. This study aims to evaluate the effectiveness of microencapsulated ethanol extract of bay leaves in reducing total cholesterol levels in white rats (Rattus norvegicus) induced by a high-cholesterol diet. The experimental study design employed was a laboratory-based true experimental method with a post-test control group design. A total of 25 male rats were used and divided into six groups: normal control, negative control (high cholesterol diet without therapy), positive control (simvastatin), and three groups treated with bay leaf extract microencapsulates at doses of 0.25%, 0.5%, and 0.75%. The treatment was given for 14 days, then total cholesterol levels were measured using an enzymatic method. The results showed that the group with a dose of 0.75% experienced the highest decrease in total cholesterol levels, namely -70 mg/dL, greater than the simvastatin group (-49 mg/dL). Statistical analysis using One Way ANOVA showed a significant difference compared to the negative control (p < 0.001). These findings indicate that the 0.75% dose of microencapsulated Syzygium polyanthum extract demonstrated an effect equal to or greater than simvastatin. Thus, it can be concluded that bay leaf ethanol extract microencapsulates are effective in reducing total cholesterol levels in hypercholesterolemic rats. This suggests that Syzygium polyanthum microencapsulation has the potential to be developed as an alternative phytotherapy for cholesterol reduction.

This study aims to comparatively analyze the effects of two types of inhalation anesthetics, ether and chloroform, on Wistar rats, focusing on three main parameters: induction time, duration of anesthesia, and side effects. Selecting the appropriate anesthetic method is crucial in animal studies to ensure animal welfare and the validity of the results. Twenty male Wistar rats, with an average body weight of 200–250 grams and 10–12 weeks of age, were randomly divided into two treatment groups of 10 rats each. The first group was anesthetized using ether, while the second group was anesthetized using chloroform, with doses adjusted to achieve adequate levels of anesthesia. Measurements were made of the induction time, which is the interval from the start of anesthetic exposure until the loss of the pedal reflex; the duration of anesthesia, which is the length of time until the rat regains consciousness; and clinical observations of any side effects that appear, both during and after anesthesia. The results showed that the ether group had a relatively longer induction time than the chloroform group. However, ether provided a longer and more stable duration of anesthesia, with a better recovery rate. In contrast, chloroform produced a shorter induction time but was accompanied by a shorter duration of anesthesia and a higher risk of side effects, particularly liver dysfunction and respiratory depression. These findings reinforce previous reports that chloroform has significant potential toxicity, and its use in animal research should be limited. Based on these results, ether is recommended as a relatively safe inhalation anesthetic option for laboratory procedures in Wistar rats, although its use still requires strict supervision and appropriate handling procedures to ensure the safety of both researchers and experimental animals.

This study aimed to compare the inhalation anesthetic profiles of ether and chloroform in white rats (Rattus norvegicus) using a modern pharmacological approach. Five healthy male rats (8–10 weeks old) were randomly divided into three groups: ether, chloroform, and control (ketoprofen). The main parameters observed were the onset time of loss of the righting reflex and the duration until its recovery. In theory, ether works by enhancing GABAergic transmission and inhibiting NMDA channels, thus having a slow but stable induction effect (IKAPI, 2009; Arqom, 2023). In contrast, chloroform works by stabilizing the neuronal membrane through activation of the K₂P TREK-1 channel and inhibition of Na⁺/Ca²⁺ currents, resulting in rapid induction with a short duration (Pavel et al., 2020). The experimental results support this theory: chloroform showed an average onset of 167.83 seconds and an anesthesia duration of 84.67 seconds, while ether had a slower onset (307.17 seconds) but a longer duration (169.33 seconds). The difference between the two was statistically significant (ANOVA, p<0.05). The coefficient of variation for chloroform was nearly four times higher than that of ether, indicating that ether provides a more consistent anesthetic effect across individuals. These findings are consistent with previous studies, such as Fathiyah & Anretha's (2023) report on the variability of chloroform effects and the results of in vivo amethyst anesthesia studies (Aprira, 2022; Genta et al., 2021). Overall, ether is more suitable for medium-term procedures requiring stable anesthesia, while chloroform is suitable for short interventions requiring rapid induction. This study emphasizes the importance of controlled inhalation environments, adequate sample sizes, and chamber standardization to enhance the external validity of the results.

Community health services are the most important part of supporting community health. Puskesmas is a health institution that is very close to the community. Therefore, it is important to pay attention to supporting facilities and infrastructure in the medical records work unit such as work equipment, work procedures, work processes or systems, work environment, employee physiology and psychology so that medical records officers more productive at work. Based on observations made at the West Baktiya Community Health Center, it was found that the area of the medical records unit was 5 x 5 m2 with a ceiling height of 3 m, using a white ceramic floor, there were 6 windows, 1 door, 2 staff work desks and 3 cupboards for Save correspondence and other important documents. Lighting in the medical records unit room is 259 lux, noise is 53.3-62.0 dB, temperature in the medical records unit room is 33.1oC and humidity is 70%. In the medical records unit room there are no chemical substances in solid, liquid or gas form, and there are also no vectors or disease-carrying animals such as flies, mosquitoes, cockroaches and rats. The aim of this activity is to provide outreach regarding ergonomics in the medical records work unit at the West Baktiya Community Health Center. The implementation method consists of planning, implementation actions, evaluation and activity report preparation stages. The result of this service is that it can increase the knowledge of medical records officers and other healt

Inflammation is a response to tissue damage due to various adverse stimuli, both chemical and mechanical stimuli and infections. One of the chemical compounds found in henna leaves is flavonoids that are efficacious as anti-inflammatory. The aim of the study was to determine the anti-inflammatory effectiveness of water henna leaf extract reviewed from a decrease in udem volume in the soles of the feet of male white rats induced by carrageenan 1%. The research method used was experimental on simplicia and ethanol extract of henna leaf (EEDPA) as well as testing the anti-inflammatory effectiveness of EEDPA. Testing of the anti-inflammatory effect was carried out on 30 male white mice induced with 1% carrageenan subplantarly. The test animal group was divided into five groups consisting of a positive group given Na-diclofenac, a negative control group given CMC, and a test group of water henna leaf ethanol extract at doses of 200 mg/kgBB, 400 mg/kgBB and 800 mg/kgBB. The parameters observed in this study were the volume of the leg udem of the test animal which was measured with a Plethysmometer every 6 hours. The data obtained was then calculated as the percent of inflammation and the percent of inflammation inhibition. Analyzed with One Way ANOVA with a 95% confidence rate. The results of the effectiveness test showed that EEDPA had an anti-inflammatory effect in carrageenan-induced male white rats. Optimal effectiveness indicated by the dose showed that EEDPA at a dose of 800 mg/kgBB

Fever is a condition characterized by an increase in body temperature as a response to infection or inflammation, and it is one of the most common clinical symptoms in various diseases. The excessive use of synthetic antipyretics such as paracetamol can lead to side effects; therefore, safer and natural-based alternatives are needed. This study aims to determine the antipyretic activity of betel leaf and to compare its effectiveness with that of paracetamol.This research was conducted using an experimental laboratory method with a completely randomized design. The test subjects were male white rats (Rattus norvegicus) of the Wistar strain, induced with fever using a 20% yeast suspension. The rats were divided into five groups: negative control, positive control (paracetamol), and three treatment groups with low, medium, and high doses of the extract. Body temperature was measured every 30 minutes for 180 minutes after treatment. The data were statistically analyzed using one-way ANOVA followed by Tukey's post hoc test.The results showed that the [plant extract] exhibited significant antipyretic activity (p < 0.05) compared to the negative control, especially at medium and high doses. The effectiveness of the highest dose was comparable to that of paracetamol. This antipyretic effect is presumed to be associated with the presence of flavonoids and tannins that may inhibit the synthesis of prostaglandin E2 (PGE2), a key mediator in the fever response.In conclusion,betel leaf has potential as an effective natural antipyretic agent and may be developed as a safer herbal-based alternative for fever treatment.

Diabetes mellitus is one of the chronic diseases whose prevalence is increasing worldwide. This disease is characterized by high blood glucose levels due to impaired insulin production or function, the hormone that regulates blood sugar levels. This study aims to examine the effect of wild betel leaf extract (Piper aduncum) on the inhibition of glucose transport using the ileum of white rats (Rattus norvegicus) with the infusion method. This research is a pre-experimental study with a posttest-only control design using white rats and wild betel leaves. The study demonstrates that wild betel leaves (Piper aduncum L.) and red betel leaves (Piper crocatum) have significant potential in inhibiting glucose transport and reducing blood sugar levels. This is evident from the significant decrease in the rate of glucose transport after the administration of wild betel leaf extract, indicating an inhibitory effect on glucose transporters such as SGLT-1 and GLUT-2.

This study explored the analgesic effectiveness of ethanol extract of red betel leaf (Piper crocatum) in white rats (Mus musculus L.) induced with acetic acid. A total of 15 rats were divided into five groups with different treatments, and the number of twitching movements as a pain response was measured for one hour. The extract was prepared using 96% ethanol solvent, and the analgesic effect was analyzed using the writhing test method. The results showed that the extract contained flavonoids, alkaloids, tannins, and saponins, which might contribute to its analgesic properties. It was found that the higher the dose of extract administered, the greater the inhibition of pain that occurred, with a dose of 800 mg/kgBB showing optimal analgesic effectiveness, almost equivalent to paracetamol as a positive control. Statistical analysis showed significant differences between treatment groups, with doses of 200 mg/kgBB and 400 mg/kgBB showing lower effectiveness. This study recommends further exploration of the therapeutic benefits of red betel leaf extract and formulation development for wider medical applications.  

This search intended to speculate the role of lavender ethanolic extract by reducing the toxicity of manganese in male rats. 32 white male Norwegian rats were divided into 4 equal groups. Group 1: as a control group left without treatments (only 1 ml of distilled water/ animal/ day). Group 2: were dosed manganese chloride at a concentration of 100 mg/ kg b.w. / day. Animals in Group 3 and Group 4 were dosed Manganese chloride at a concentration of 100 mg/kg b.w. then lavender ethanolic extract at a concentration of 200 and 400 mg/kg respectively, all by oral gavages and treated daily for six weeks. Then the next criteria were investigated: Neurotransmitters (dopamine and Ach (acetylcholine) in the mid brain) serotonin in serum. Oxidation indicators (glutathione and malondialdehyde in the brain and superoxide dismutase and catalase in serum). The results exhibited in both groups 3 and 4 there were a significant decrease in the concentrations of Ach accompanied by a significant increase in levels of dopamine and serotonin, also return levels of MDA (Malondialdehyde) to normal, as well as a significant increase in GSH (Glutathione) concentrations in rats mid brain. Moreover, the significant rising of SOD (Superoxide dismutase) and CAT (Catalase) levels in serum of rats in these groups indicated a noticeable improvement was achieved by lavender ethanolic extraction as compared to group 2. Conclusion, the antioxidant and antitoxic activity of lavender ethanolic extract promises in grate achievement in various health fields, including medicine, food industries and cosmetics.

Liver damage induced by hepatotoxic compounds such as N-acetyl-p-aminophenol (N-APAP) remains a significant global health concern. Hepatotoxicity caused by N-APAP, particularly in cases of overdose or prolonged use, can lead to a reduction in glutathione levels in the liver, resulting in oxidative stress and cellular damage. This condition contributes to an increased incidence of liver diseases, including toxic hepatitis and acute liver failure, which can lead to serious complications and even death. Although various pharmacological therapies, such as N-acetylcysteine (NAC), are available to address N-APAP-induced liver damage, limitations such as side effects, high costs, and variable efficacy have driven the search for safer and more affordable therapeutic alternatives. Therefore, research on natural hepatoprotective agents, such as extracts from the rhizomes of fingerroot (Boesenbergia rotunda), has become increasingly relevant in addressing this issue. The aim of this study was to evaluate the hepatoprotective potential of ethanol extract from fingerroot rhizomes (Boesenbergia rotunda) in protecting the liver from N-acetyl-p-aminophenol (N-APAP)-induced damage. Specifically, this research focuses on measuring glutathione levels as a key indicator of the liver's protective mechanism against oxidative stress. In this study, 30 male White rats were randomly divided into six groups, each consisting of five rats. All rats were orally administered the extract at predetermined doses for 10 consecutive days, except for the negative and positive control groups. On the 10th day, all groups (except group 1) were treated with paracetamol (800 mg/kg body weight) to induce liver damage. Twenty-four hours after induction, the rats were sacrificed and liver samples were collected. Glutathione levels were measured using the ELISA immunosorbent assay. Statistical analysis showed that administering the ethanol extract of Fingerroot rhizomes (EERTK) for 10 days prior to N-APAP induction significantly increased the average antioxidant enzyme GSH-Px levels in the liver homogenate of rats in the EERTK 250 mg/kg BW (43.36 ± 1.34 U/mg protein), EERTK 500 mg/kg BW (55.70 ± 2.15 U/mg protein), and 750 mg/kg BW (53.14 ± 2.57 U/mg protein) group compared to the negative control group (N-APAP 800 mg/kg BW), which had the lowest average antioxidant enzyme GSH-Px level (33.86 ± 3.52 U/mg protein).

Takokak fruit contains bioactive compounds that can be used as antidiabetics. This study aims to identify bioactive compounds of ethanol extract of takokak fruit and antidiabetic test of n-Hexane fraction. The method used to obtain the extract is maceration using ethanol . Antidiabetic testing is carried out through in vivo testing with the Oral Glucose Tolerance Test (OGTT) method on white rats. Secondary metabolites are compounds contained in taakokak fruit that are identified as flavonoids, saponins, tannins, steroids and terpenoids . The results of the antidiabetic test showed that the n-Hexane fraction with glibencamide as a comparator has the ability as an antidiabetic in terms of the amount of difference in blood glucose levels in the test animal group from T 30 to T 120 . The average decrease in blood glucose levels in the negative control group (Na-CMC) was 108.67 mg/dL, the positive control group ( glibencamide ) 174.67 mg/dL, the 5% concentration fraction group 35.33 mg/dL, the 10% concentration fraction group 30.67 mg/dL, the 20% concentration fraction group 47.67 mg/dL.

Arumanis mango rind is an organic waste that contains secondary metabolites such as flavonoids. Many flavonoids are found in the rind of the arumanis mango, which acts as a very strong antioxidant and has the potential to heal wounds, including infected wounds. This study aims to determine the effect of arumanis mango (Mangifera indica L.) rind extract ointment on healing infected wounds caused by Pseudomonas aeruginosa bacteria, determine the effective concentration for healing infected wounds, and the histopathological decripstions. This research was an experimental study using male white rats which were divided into 5 groups, namely group I (control), group II (Gentamicin), group III (5% concentration), group IV (10% concentration) and group V (15% concentration). The parameters observed were the percentage of healing of infected wounds, epithelialization time, and histopathology. Then the data were analyzed using the ANOVA test followed by Duncan's further test.The results of the study were based on the group order above, from the parameters the average percentage of infected wound healing on 3rd day  was 29.73%; 48.84%; 45.95%; 42.81%; 42.33%, on 7th day it was 70.96%; 85.79%; 83.25%; 76.95%; 73.87%, and on the 14th day it was 89.89%; 93.32%; 93.87%; 92.67%; 91.48%. Epithecization time is 9; 6; 7; 8; 9 (day), for histopathology of fibroblast cells with a score of 1; 3; 3; 2; 2; for collagen fibers 1; 3; 3; 3; 3; for re-epithelialization 2; 3; 2; 2; 2; for inflammatory cells 0; 3; 2; 2; 2. The conclusion of the research is that arumanis mango peel extract ointment (Mangifera indica L.) has an influence on the healing process of wounds infected by Pseudomonas aeruginosa bacteria in male white mice, the most effective concentration and the best histopathological descriptions at a concentration of 5%.  

Takokak fruit is one of the plants that can be used to lower blood sugar levels. This study aims to identify the bioactive compounds of ethanol extract of takokak fruit and test the antidiabetic ethyl acetate fraction. Antidiabetic testing was carried out by in vivo testing using the Oral Glucose Tolerance Test (OGTT) method to see the decrease in blood glucose levels using white rats as test animals. The test results showed that takokak fruit contains flavonoids, steroids, and tannins. Fractionation is the process of extracting compounds from the extract using two types of solvents that do not mix with each other. The results of the antidiabetic test showed that the ethyl acetate fraction with glibenclamide as a comparator had the ability as an antidiabetic measured by the amount of difference in the decrease in blood glucose levels in the test animal group T₃₀ to T₁₂₀. From the results obtained, the average decrease in blood glucose levels in the negative control group (Na CMC) was 5.8 mg/dL, the positive control group (Glibenclamide) 21.03 mg/dL, and the fraction group 9.86 mg/dL. Takokak fruit has antidiabetic activity. Compounds that have antidiabetic activity are flavonoids, tannins, and steroids.

Examination of serum urea and creatinine levels is an important indicator in assessing the physiological function of the kidneys. Consumption of durian, which is rich in potassium, may increase the risk of hyperkalemia, especially in patients with chronic kidney disorders. One of the popular durian species in Indonesia is Durio zibethinus, which is native to the forests of Sumatra, Malaysia and Kalimantan. Its complex nutritional content may affect kidney function if consumed in excess. This study aims to evaluate the effect of durian (Durio zibethinus) pulp administration on changes in kidney function, especially urea and creatinine levels, in male Wistar white rats (Rattus norvegicus L.). This study used a true experimental design with a posttest-only design with control group design. A total of 24 rats were divided into four groups: negative control, positive control, and two treatment groups with durian fruit pulp in graded doses for 28 days. Measurement of ureum and creatinine levels was performed, and data analysis used One-Way ANOVA test with Bonferroni and Kruskal-Wallis post hoc. Durian administration in graded doses in the treatment group showed a significant effect on increasing ureum and creatinine levels compared to the negative control group (p < 0.05). However, there were no significant differences between treatment groups (P1, P2, and P3) (p> 0.05). Administration of durian fruit pulp at graded doses has a significant effect on increasing ureum and creatinine levels, indicating changes in kidney function in Wistar rats. This finding underscores the importance of regulating durian consumption, especially for individuals at risk of renal impairment.

This research tested the effect of cadmium chloride and lead acetate on kidney function and their impact on kidney tissues. The study included (30) white rats that were randomly divided into (6) groups:- The control group takes plain water orally for 30 days, the group that was given CdCl2 (5 mg/kg) of body weight, the group that was given CdCl2 10 mg/kg of body weight, the group that was given lead acetate 50 mg/kg of body weight, the group that was given lead acetate 100 mg/kg of body and the group that was given cadmium chloride 5mg/kg with lead acetate 50 mg/kg body weight with drinking water for a month .The results shown in Table (1) indicated a significant increase (P < 0.05) in the levels of urea, creatinine, and uric acid in the serum of groups treated with CdCl2 at doses of 5 and 100 mg/kg, and the groups treated with lead acetate at doses of 50 and 100 mg/kg, as well as the group that received CdCl2 at a dose of 5 mg/kg along with lead acetate at a dose of 50 mg/kg, comparison with the healthy group .As for histological sections, the results showed the following section of the renal of the group treated with cadmium chloride 5 mg/kg shows atrophic renal glomerulus, necrosis of cells lining Bowman’s capsule , necrosis of cells lining the convoluted tubule  and a large increase in the space between the glomerulus and the capsule. As for the group treated with high-dose cadmium chloride showing necrosis of cells lining the glomerulus  and degeneration of cells lining the renal tubules Fragmentation of the glomerulus. As for the group treated with lead acetate at a dose of 50 mg/kg showing renal glomerulonephrosis, proximal and distal convoluted tubule necrosis, and periglomerular space in an almost normal manner. A section of the kidney of the group treated with with high-dose lead acetate, showing atrophy of some renal glomeruli and congestion of blood vessels and showing blood congestion within the kidney tissue, and decomposition of the cells lining the urinary tubules , Cross-section of the kidney of the group treated with cadmium chloride 5 mg/kg and lead acetate 50 mg/kg body weight Shows destruction of renal glomeruli  and swelling of renal tubule cells and necrosis of the kidney tissue with the appearance of hemorrhage Comparison with the healthy group

Clove leaves have the property of overcoming hair growth problems, where the eugenol compound contained in clove leaves is efficacious as a hair growth agent. This research was conducted to formulate a clove leaf extract shampoo preparation. The research method used was the experimental method. Tests were carried out on experimental animals, namely male white mice in various groups. The first group (without treatment), the second group (negative control), the third group (5% clove leaf extract shampoo), the fourth group (10% clove leaf ethanol extract shampoo), the fifth group (15% clove leaf ethanol extract shampoo) and groups sixth (positive control). The shampoo preparations were tested and evaluated, the results of the preparation evaluation test showed that the four shampoo preparations were homogeneous and had a pH ranging from 5.1-5.7, with a viscosity of 1700-3000 cps and a foam height of 9-11 cm. This shampoo preparation also does not irritate the skin. The results of the data analysis obtained showed that all data were normally distributed (p> 0.005) and homogeneous (p> 0.005). The results of the one way ANOVA test show that F1 (5% formula), F2 (10% formula) and F3 (15% formula) have significant differences. This research shows that clove leaf ethanol extract shampoo with a concentration of 15% is the most effective formulation in hair growth in male white rats. However, the ethanol extract of clove leaves shampoo is less effective than minoxidil 5% for hair growth.