Publication Search

Bulian coconut chips are a typical Jambi food made from dried and fried old coconuts. The traditional production method and inadequate hygiene standards make this product susceptible to microbial contamination, especially harmful bacteria. This study aims to identify the types of bacteria that may be present in Bulian coconut chips using culture and Gram staining methods as the initial step for morphological identification. Samples were taken from three local producers in Bulian District, Batanghari Regency, Jambi. Testing was carried out by serial dilutions up to 10⁻⁴, then planted on Nutrient Agar (NA) media and incubated for 24 hours at 37°C. The colonies that grew were then observed morphologically and Gram staining was performed using a modified method without alcohol. The results showed the presence of various colonies with cream, white, and pale yellow colors. Based on the results of Gram staining, bacilli and cocci were found, which were included in the Gram-positive and Gram-negative categories. The types of bacteria suspected to appear include Bacillus sp., Staphylococcus sp., and possibly Escherichia coli. These findings indicate a risk of microbial contamination that could endanger consumer health, especially if the production process is not improved. Strict hygiene and sanitation principles are strongly recommended in the processing of this traditional food to ensure the product remains safe for consumption. The importance of implementing hygienic standards in the production of Bulian coconut chips is further emphasized by these findings. In addition to improving food safety, implementing proper sanitation procedures can also extend the product's shelf life and increase consumer confidence in local traditional foods. Efforts such as training local producers, using clean equipment, and hygienic packaging can help minimize the risk of bacterial contamination.

CBT Room 2 at the Faculty of Medicine, Universitas Muhammadiyah Purwokerto, is used for computer-based lectures and practicums, which may lead to exposure to pathogenic microorganisms from device surfaces, such as computer mice. This study aims to identify pathogenic bacteria and fungi present on mouse surfaces and to evaluate the influence of room temperature and humidity on microbial growth. A total of 60 mouse samples were tested using Nutrient Agar (NA) for bacteria and Sabouraud Dextrose Agar (SDA) for fungi. The recorded room temperature was 25.6°C with a humidity level of 74%. The identification results revealed the presence of Bacillus sp., Escherichia coli, Staphylococcus sp., and Streptococcus sp. on NA media, with Gram-positive bacteria dominating (78.33%). Meanwhile, Aspergillus sp. (40.00%) and Penicillium sp. (38.33%) were found on SDA media. These findings indicate that, although the room's temperature and humidity are not at optimal levels, they still support the growth of mesophilic microbes. The implications suggest the need for routine sterilization efforts to prevent cross-infection risks in the learning environment.  

Daging merupakan salah satu produk makanan yang bernilai gizi tinggi. Umumnya daging segar juga mudah rusak seiring dengan berjalannya waktu berjualan para perdagang. Tujuannya untuk mengetahui jenis mikroorganisme yang tumbuh pada daging dan juga laju dari pertumbuhan mikroorganisme tersebut pada daging. Metoda Analisa yang digunakan adalah jenis Identifikasi Mikroorganisme dan juga laju pertumbuhan mikrobia pathogen. Serta Analisa statistika menggunakan geometric mean. Berdasarkan hasil uji identifikasi mikrobia pathogen maka di dapatkan 2 jenis bakteri yaitu Bacillus. Sp dan Staphylococcus sp. Dimana morfologi bacillus dalam media selektif. Hal ini terlihat dari bentuk koloni yang besar-besar, koloninya menyebar atau dalam bentuk rantai, serta terbentuknya hemolisis pada beberapa spesies bacillus dalam media Blood Agar Plate. Sedangkan Staphylococcus sp di tandai dengan terbentuknya warna reddish-purple pada media tersebut yang menunjukkan karakteristik dari warna staphylococcus. Jadi dapat disimpulkan bahwa Bacillus sp dan Staphylococcus sp umumnya hidup pada daging segar yang berada di suhu ruang. Sebaiknya daging segar disimpan pada suhu freezer agar tidak mudah rusak oleh bakteri patogen.