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Limited land availability is a major constraint in developing school infrastructure in active urban educational facilities. This study evaluates the classroom development plan at SDN 39 Lubuklinggau from the perspective of a planner, focusing on land limitation, functional needs, safety, constructability, and long-term building performance. A descriptive-evaluative approach was applied through technical observation, document review, alternative assessment, weighted scoring, and risk analysis. The results indicate that horizontal expansion is constrained by limited open space and may reduce internal circulation, student activity areas, and emergency space. Three alternatives were compared: horizontal expansion, building rearrangement, and a two-storey classroom scheme. The two-storey alternative obtained the highest score of 84, compared with 61 for rearrangement and 56 for horizontal expansion. However, its feasibility depends on further soil investigation, structural design, staircase and evacuation safety, utility coordination, construction phasing, and strict site safety control. The study recommends that the planner prioritize detailed site measurement, geotechnical investigation, detailed engineering design, construction safety planning, and post-construction functional evaluation. A vertical classroom strategy can be considered a rational solution for land-constrained schools when supported by accountable technical planning and integrated project control.

Lead (Pb²⁺) contamination in wastewater is a major environmental concern due to its toxicity, persistence, and ability to accumulate in living organisms. Adsorption using biomass-based materials has been developed as an alternative treatment method because it is efficient, economical, and environmentally friendly. This study aimed to analyze the effect of bioadsorbent dosage and contact time on Pb²⁺ removal efficiency and evaluate Pb²⁺ mass distribution using mass balance analysis in a batch adsorption system. Ramie leaf (Boehmeria nivea) was utilized as a bioadsorbent due to its lignocellulosic content, which provides active sites for metal ion adsorption. The research was conducted experimentally through bioadsorbent preparation, chemical activation, and batch adsorption using artificial Pb²⁺ solutions with variations in bioadsorbent dosage and contact time. Pb²⁺ concentrations before and after adsorption were analyzed using Atomic Absorption Spectroscopy (AAS). The obtained data were used to determine removal efficiency and Pb²⁺ distribution between the liquid phase and bioadsorbent. The results showed that ramie leaf bioadsorbent effectively reduced Pb²⁺ concentration, with the optimum condition obtained at 4 g/L bioadsorbent dosage and 90 minutes contact time, achieving 98.46% removal efficiency. Mass balance analysis indicated that Pb²⁺ ions were successfully transferred from the solution phase onto the bioadsorbent surface. These findings demonstrate that ramie leaf bioadsorbent has potential as a sustainable alternative material for heavy metal removal in wastewater treatment.

Online transportation has become an essential component of modern urban mobility, significantly transforming the way people travel while creating new challenges related to service quality, safety, customer satisfaction, sustainability, and its impact on conventional public transportation. This study aims to examine the development of research on online transportation through a bibliometric analysis combined with a Systematic Literature Review (SLR). Data were collected from the Scopus database using the keyword "Online Transportation" and filtered using the PRISMA framework, resulting in 90 eligible articles for analysis. The bibliometric analysis was conducted using VOSviewer to identify publication trends, leading contributing countries, author affiliations, journal sources, and the most frequently occurring keywords. The findings reveal that research on online transportation remains relatively limited despite showing a gradual increase in recent years. Indonesia emerged as the country with the highest number of publications, followed by the United States, China, Japan, Malaysia, and the United Kingdom. Frequently occurring keywords indicate that online transportation research is commonly associated with traffic management, drivers, passenger safety, customer satisfaction, service quality, employment, and user loyalty. These findings suggest substantial opportunities for future studies, particularly in the areas of regulatory frameworks, sustainable transportation, traffic safety, digital technology integration, and the development of intelligent transportation systems to support more efficient, inclusive, and sustainable urban mobility.

This community service program (PkM) aims to enhance the digital literacy of teachers and students at Pondok Pesantren Al-Wathoniyah Pusat Putri Klender, East Jakarta, particularly in verifying digital news and content amid pervasive disinformation. Key problems identified include low digital verification competency among senior teachers, limited use of fact-checking tools, and the absence of systematic information management mechanisms within the pesantren. The activity was held on June 10, 2026, employing a blended learning approach combining interactive lectures, hands-on training, and continuous mentoring. Training modules covered: (1) digital literacy for teachers, (2) mitigation of religious information distortion, (3) critical media literacy, (4) videography skills, and (5) content marketing grounded in Islamic values. Thirty teachers and senior students participated. Key outputs include enhanced digital literacy competencies among participants, the Smart Teacher Guidelines module, a donated photography studio equipment set, and national media coverage (JPNN.com). Evaluation results demonstrate significant improvement in participants' understanding of digital information verification, positive content production capability, and motivation to use technology responsibly. This program contributes to SDGs No. 4 (Quality Education), SDGs No. 9 (Innovation), SDGs No. 10 (Reduced Inequalities), and SDGs No. 17 (Partnerships), as well as supporting IKU 3, IKU 5, and IKU 6 of Universitas Negeri Jakarta.

This study analyzes the effect of logistics digitalization systems and personnel competence on the distribution of personnel uniforms (kaporlap) at Kodiklatal. The study was motivated by the fact that the distribution system had not yet operated optimally despite the implementation of digital logistics, while delays in updating the Sistem Informasi Entitas Samapta (SIMBENSAM), limited documentation of device identities, and unequal digital skills among personnel still constrained distribution performance. A quantitative approach with explanatory design was applied. The population consisted of 3,099 personnel and the sample comprised 97 respondents selected using Slovin's formula. Data were collected through Likert-scale questionnaires and internal documentation, then analyzed using validity and reliability tests, classical assumption tests, descriptive analysis, and simple and multiple linear regression. The results show that the logistics digitalization system has a positive and significant effect on kaporlap distribution (t = 24.607; p < 0.05), personnel competence also has a positive and significant effect (t = 37.972; p < 0.05), and both variables simultaneously affect distribution performance (F = 788.285; p < 0.05) with an R square of 0.944. These findings confirm that digital systems and human competence must be strengthened in an integrated manner to support efficient, accurate, and timely distribution in a military education environment.

The KRI Nagapasa Class is a submarine of the Indonesian Navy that has the capability to deploy sea mines, launch anti-surface missiles, and release countermeasure torpedoes. However, to face the increasingly complex dynamics of the regional strategic environment, it is necessary to increase combat power through the installation of a Sub Launch Missile weapon system. Until now, the Indonesian Navy does not have a submarine equipped with this underwater missile, especially in the Chang Bogo class. This study aims to analyze and determine the most appropriate choice of Sub Launch Missile missiles to support submarine training and operations. The method used is the Analytic Hierarchy Process (AHP) with a quantitative approach. The results of the ranking of five respondents showed that the SM-39 Exocet missile was in first place with a value of 0.46424 followed by the CM-708UNB with a value of 0.41461 and the UGM-84 Harpoon with a value of 0.12115. The SM-39 Exocet missile was chosen as a priority because it has technical specifications that best meet the operational needs of the Indonesian Navy (TNI AL), as well as significantly contributing to enhancing the deterrence effect and combat capability of the Nagapasa-class submarines.

The Indonesian Archipelagic Sea Lane II (ALKI II) constitutes a strategic route stretching from the Sulawesi Sea to the Indian Ocean with trade values reaching USD 1.5 million daily and traversed by more than 36,773 vessels annually. The complexity of maritime security threats in this region has increasingly intensified following the relocation of the capital city to East Kalimantan, thereby demanding an effective decision support system to assist Indonesian Warship Commanders in making operational decisions that are rapid, accurate, and in accordance with applicable legal provisions. This research employs a qualitative approach through in-depth interviews with four maritime expert sources, with data analysis using NVivo 12 software. Analysis results identified maritime operational complexity and the urgent need for a comprehensive decision support system. These findings subsequently served as guidance in designing a Decision Support System (DSS) with a tab-based interface that integrates international legal frameworks such as UNCLOS 1982, Law No. 3/2025 on TNI, and related national regulations to generate structured action recommendations along with their legal foundations. Research findings demonstrate that the developed DSS successfully integrates operational, legal, and situational aspects to support decision-making that is rapid, accurate, and legally compliant. This system has proven to enhance the effectiveness of Indonesian Warship Commanders in addressing the complexity of maritime security (KAMLA) challenges in ALKI II through the provision of legally-based action recommendations from national and international frameworks accessible in realtime during operational situations.  

Esophageal atresia with tracheoesophageal fistula is a congenital anomaly requiring early diagnosis and prompt surgical correction. Gross type D esophageal atresia, characterized by both proximal and distal tracheoesophageal fistulas, is an exceedingly rare variant that poses significant diagnostic and operative challenges. We report a case of a full-term female neonate with a birth weight of 3,200 grams who presented with hypersalivation since the first day of life, accompanied by coughing and choking during feeding. Orogastric tube insertion failed, with the tube arrested at approximately 15 cm. The patient was referred with a diagnosis of neonatal pneumonia and suspected esophageal atresia with tracheoesophageal fistula. Physical examination revealed tachypnea, chest wall retraction, and respiratory distress requiring ventilatory support. Babygram demonstrated the orogastric tube tip arrested at the proximal esophageal pouch with distal gastrointestinal air. Contrast esophagography confirmed proximal esophageal atresia at the upper thoracic level with suspected proximal fistula. Associated findings included bilateral pneumonia, right upper lobe atelectasis, and vertebral anomalies suggestive of VACTERL association. Intraoperative findings confirmed Gross type D esophageal atresia with proximal and distal tracheoesophageal fistulas. Both fistulas were excised, followed by primary esophageal anastomosis and transanastomotic nasogastric tube placement. Gross type D esophageal atresia is a rare congenital anomaly that should be considered in neonates presenting with hypersalivation, failed orogastric tube insertion, and respiratory distress. Anatomical subtype confirmation relies on intraoperative findings. Early surgical repair can be performed successfully; however, associated anomalies necessitate careful long-term follow-up.

Background: Adolescence is often characterized as a period of puberty. Early menarche is associated with increased mortality and morbidity risks in later life among girls who experience menarche at an early age. Menarche is the first menstrual event in girls, marked by the shedding of the uterine lining and vaginal bleeding. The tendency to consume unhealthy foods, particularly fast food, as well as sugar-sweetened beverages (SSBs), may have adverse effects on health and potentially influence the timing of menarche. Objective: This study aimed to analyze the relationship between healthy food selection, sugar-sweetened beverage consumption, and the incidence of early menarche. Methods: This study employed a cross-sectional design. The sample consisted of 30 female students. Data were analyzed using the Spearman Rank correlation test. Results: The results showed that healthy food selection was significantly associated with the incidence of early menarche (p = 0.005) with a moderate correlation strength (ρ = 0.449). Similarly, sugar-sweetened beverage consumption was significantly associated with the incidence of menarche (p = 0.000) and demonstrated a very strong correlation (ρ = 0.841). Conclusion: There was a significant relationship between healthy food selection and sugar-sweetened beverage consumption with the incidence of menarche. Sugar-sweetened beverage consumption showed a stronger relationship with menarche incidence compared to healthy food selection.

Personal hygiene practices during menstruation is important to maintaining the reproductive health of adolescent girls and preventing reproductive organ infections. Poor hygiene causes post-menstrual problems in 5.2 million adolescents in Indonesia, such as reproductive tract infections, urinary tract infections, pathological vaginal discharge, fungal infections, genital area irritation, and unpleasant odor. Factors influencing personal hygiene practices during menstruation include knowledge, perceptions, attitudes, and infrastructure. This study was aimed determine the relationship between knowledge, perceptions, attitudes, and infrastructure and personal hygiene practices during menstruation among female students at the Daarussa'adah Qur'an Tahfidzil Islamic Boarding School in Pesawaran in 2026. This study was quantitative with a cross-sectional approach. The population was all female students at the Daarussa'adah Qur'an Tahfidzil Islamic Boarding School in Pesawaran, and a sample of 74 respondents was selected using total sampling. Data analysis used the chi-square test with a significance level of 95%. The results of the study showed a relationship between knowledge (p-value = <0.001; OR = 279.000), perception (p-value = <0.001; OR = 620.000), attitude (p-value = <0.001; OR = 165.333), and infrastructure (p-value = <0.001; OR = 0.002) and personal hygiene behavior during menstruation. Suggested for the Islamic boarding schools include routinely improving reproductive health education regarding personal hygiene during menstruation and providing adequate infrastructure to support the development of good knowledge, perceptions, and attitudes, so that female students can optimally implement personal hygiene behaviors during menstruation.

Diabetes Mellitus (DM) is a metabolic disease with an increasing prevalence and a risk of causing macrovascular complications such as stroke. This study aimed to describe the characteristics of Diabetes Mellitus patients with stroke complications based on CT-scan results at RSKD Dadi Makassar in 2024–2025. This was a descriptive observational study with a retrospective design using medical record data. Samples were taken using a total sampling technique, comprising 60 patients, and analyzed univariately using the Statistical Product and Service Solutions (SPSS) version 26. The results showed that the majority of respondents were in the late elderly group (>56 years) at 66.7%, female (51.7%), and from the Makassar ethnic group (63.3%). Most respondents experienced hyperglycemia in random blood glucose (66.7%) and had uncontrolled fasting blood glucose (68.3%) and HbA1c (76.7%) levels. CT-scan results were dominated by non-specific cerebral infarction (68.3%), with ischemic stroke as the most common type (93.3%), a length of stay of 5–10 days (53.3%), and right-sided hemiparesis as the most common clinical manifestation (45.0%). It was concluded that DM patients with stroke complications were generally elderly, female, had poor glycemic control, and were dominated by ischemic stroke with non-specific cerebral infarction. Abstrak. Diabetes Melitus (DM) merupakan penyakit metabolik dengan prevalensi yang terus meningkat dan berisiko menimbulkan komplikasi makrovaskular berupa stroke. Penelitian ini bertujuan untuk mengetahui gambaran karakteristik pasien Diabetes Melitus yang mengalami komplikasi stroke berdasarkan hasil CT-scan di RSKD Dadi Makassar tahun 2024–2025. Penelitian ini merupakan penelitian observasional deskriptif dengan desain retrospektif menggunakan data rekam medis. Sampel diambil dengan teknik total sampling sebanyak 60 pasien dan dianalisis secara univariat menggunakan Statistical Product and Service Solutions (SPSS) versi 26. Hasil penelitian menunjukkan mayoritas responden berusia lansia akhir (>56 tahun) sebanyak 66,7%, berjenis kelamin perempuan (51,7%), dan berasal dari Suku Makassar (63,3%). Sebagian besar responden mengalami hiperglikemia pada GDS (66,7%) serta memiliki kadar GDP (68,3%) dan HbA1c (76,7%) yang tidak terkontrol. Hasil CT-scan didominasi oleh infark serebri tidak spesifik (68,3%) dengan jenis stroke terbanyak berupa stroke iskemik (93,3%), lama rawat inap terbanyak 5–10 hari (53,3%), dan manifestasi klinis tersering berupa hemiparese dextra (45,0%). Disimpulkan bahwa pasien DM dengan komplikasi stroke umumnya berusia lanjut, berjenis kelamin perempuan, memiliki kontrol glikemik yang buruk, dan didominasi oleh stroke iskemik dengan gambaran infark serebri tidak spesifik.

Nursing care in hospitals is still below the established standard of 80%, thus reflecting suboptimal nurse performance. This condition is thought to be influenced by factors such as rewards, workload, length of service and supervision. This study aims to analyze the relationship between rewards, workload, length of service, and supervision with nurse performance in the Inpatient Unit of Ratu Zalecha Martapura Regional Hospital. This study used a quantitative method with an observational analytical design using a cross-sectional approach. The study sample consisted of 152 nurses selected using cluster proportional random sampling technique. Data were collected using a questionnaire and analyzed using the chi-square test and multiple logistic regression. The results showed a significant relationship between rewards and nurse performance (p-value=<0.001), workload and nurse performance (p<0.001), and supervision and nurse performance (p-value=<0.001). Length of service did not show a significant relationship with nurse performance (p-value=0,968). Multivariate analysis showed that supervision was the most dominant factor related to nurse performance. Thus, compensation, workload, and supervision are related to nurse performance, while length of service is not related to nurse performance in the Inpatient Unit of Ratu Zalecha Regional Hospital, Martapura. The hospital is expected to improve supervision, workload management, and training and recognition for nurses to improve the quality of nursing services.

Aromatherapy is the use of essential oils derived from natural materials to improve physical and psychological well-being. One of the most popular aromatherapy products is the aromatherapy candle, which provides relaxation while creating a comfortable atmosphere. This study aimed to develop aromatherapy candles based on agarwood (Aquilaria malaccensis) leaf extract, evaluate product quality, analyze public acceptance, and identify their potential as an entrepreneurial opportunity. This study employed an experimental method with a descriptive approach. The research stages included the extraction of agarwood leaves through a distillation process, the formulation of aromatherapy candles, product validation by three experts using sensory evaluation, and a public acceptance test involving 40 respondents using a Likert-scale questionnaire. The results showed that the third experiment produced the best extract characteristics, including a golden-yellow color, a stronger distinctive agarwood aroma, and an oily texture. The public acceptance test indicated that the average scores for color, texture, ease of use, and aroma were 3.16, 2.90, 3.42, and 3.02, respectively, which were categorized as good to very good. These findings demonstrate that agarwood leaf extract has the potential to be used as an alternative ingredient in the production of aromatherapy candles, in addition to receiving a high level of public acceptance, the product possesses economic value and can be developed as an innovative product and entrepreneurial opportunity in the beauty, spa, wellness, and creative industries.

A bustier plays a crucial role in supporting the body, enhancing its shape, and enhancing aesthetics, especially when worn with a kebaya or evening gown. This study aims to analyze the results of bustier production using three different types of boning, such as needle-inserted boning, sewn-in boning, and steel boning, to determine the most optimal type of boning. The method used is Research and Development (R&D), encompassing data collection, product design, validation, testing, and product revision. This study found that the bustier with inserted boning achieved fairly good results with an average score of 3.00, which equates to 75.00% and falls into the “acceptable” category, while the bustier with sewn-in boning demonstrated the most optimal results. Based on validation testing by two expert validators, this product received an average score of 3.64 out of a total of 4, with a 90.91% acceptability rate, which falls into the “highly acceptable” category. The bustier with steel boning received an average score of 2.82, or 70.45%, which also falls into the “acceptable” category. Steel boning is not yet widely available, so its availability should be confirmed before production begins. Installing both sewn boning and steel boning requires precision. Therefore, it is recommended that you first practice structural sewing techniques.

The purpose of this study was to determine the learning outcomes of second-semester students in solid hair trimming skills after receiving learning through compression methods using video-based media or compression methods without media, also to analyze how influential video media in compression methods are on student learning outcomes on trimming material. The following study is a type of quasi-experimental research. With the results showing that the average post-test score of experimental class students, who were given learning using the exposure method with the help of video media on solid hair trimming material, was 83.93. On the other hand, the control class, which was given demonstration learning without video media. The average post-test score was 79.30. The results of the Hypothesis test showed a calculated value greater than the t table (4.158 > 2.00) and a significance value of 0.0000 smaller than 0.05. Therefore, it can be concluded that explanations with the help of video media have an influence on learning outcomes in solid hair trimming in second-semester students. In addition, this method has an effect of increasing by 5.84% on learning outcomes compared to the demonstration method without media.Keywords: Demonstration Method, Video, Solid Hair Cutting

Embroidery is one of Indonesia’s traditional craft arts that has developed over time and continues to attract people from various social backgrounds. Embroidery techniques are commonly applied to fashion products as well as household items. The diversity of embroidery motifs and techniques provides aesthetic value and uniqueness to each product, making embroidery an important element in the development of the fashion industry. This opportunity can encourage students of the Fashion Design Program at AKS Ibu Kartini to develop their entrepreneurial potential through creativity in manual embroidery. This study employed a quantitative approach using multiple linear regression analysis as the data processing technique to determine the influence of internal factors on the entrepreneurial motivation of Fashion Design students at AKS Ibu Kartini. The research sample consisted of 65 respondents. The findings revealed that, simultaneously, the four independent variables had a significant effect on entrepreneurial motivation, with a coefficient of determination (R²) value of 0.910. This indicates that 91% of the variation in entrepreneurial motivation can be explained by technical knowledge (X1), technical skills (X2), creativity and innovation (X3), and entrepreneurial mindset (X4), while the remaining 9% is influenced by other factors outside this study. Partially, variables X1, X2, and X4 showed a positive and significant effect on entrepreneurial motivation, whereas X3 demonstrated a negative and significant effect. This finding indicates that creativity without market orientation may reduce students’ motivation to engage in entrepreneurship. The implications of this study emphasize the importance of improving students’ knowledge, skills, and entrepreneurial mindset. Furthermore, proper guidance is needed to ensure that students’ creativity and innovation are aligned with market demands in order to optimally enhance entrepreneurial motivation.

The expensive Siger accessories make beginner MUAs or beauty students spend money on renting Siger accessories during practice. Therefore, the author is looking for alternative methods to produce cheaper accessories with maximum results. The goal is to understand the production process, assess the feasibility level, and determine the level of public preference for Siger Wedding accessories from hot glue. A descriptive quantitative research method is used, including methods such as observation, literature review, experiments, documentation, and questionnaires. The production process includes several stages: making the frame, creating patterns, and coloring, which uses two experiments with aerosol paint and decorative paint. The product feasibility was assessed by 5 expert validators. Product A received a score of 119, while product B scored 115, indicating that product A is more viable. Additionally, results from a questionnaire completed by 30 respondents show that public preference for the accessories is very high.

Science and language literacy remain challenges in non-formal education, as learning often emphasizes memorization and underutilizes local contexts as learning resources. This community service program aimed to strengthen the competence of Community Learning Center (PKBM) tutors in developing science and language literacy modules based on the ethnoscience and local wisdom of Klaten. The program employed a Training of Trainers (ToT) approach involving training sessions, module development workshops, and mentoring. Participants consisted of 20 tutors from three PKBM in Klaten Regency. Competence was evaluated through pretest and posttest questionnaires covering literacy understanding, pedagogical competence, module development, and learning implementation. Results indicated a 24,61%. increase in tutor competence, with the average score rising from 63,31 to 87,92. The program effectively enhanced tutors’ capacity to design contextual learning grounded in ethnoscience and local wisdom.

Prasman (Peperomia pellucida) merupakan tanaman obat yang diketahui mengandung berbagai senyawa metabolit sekunder seperti alkaloid, flavonoid, saponin, tanin, dan terpenoid yang berpotensi memiliki aktivitas biologis. Penelitian ini bertujuan untuk mengetahui tingkat toksisitas ekstrak metanol batang prasman terhadap mortalitas larva udang (Artemia salina L.) menggunakan metode Brine Shrimp Lethality Test (BSLT). Penelitian dilakukan secara eksperimental dengan memberikan ekstrak metanol batang prasman pada berbagai konsentrasi, yaitu 10, 100, 500, 1000, 5000, dan 10000 ppm serta kontrol. Parameter yang diamati adalah persentase mortalitas larva setelah 24 jam perlakuan. Data dianalisis menggunakan metode interpolasi dan analisis probit untuk menentukan nilai LC₅₀. Hasil penelitian menunjukkan bahwa peningkatan konsentrasi ekstrak menyebabkan peningkatan mortalitas larva secara bertahap hingga mencapai 100% pada konsentrasi 10.000 ppm. Perhitungan LC₅₀ dengan metode interpolasi menghasilkan nilai 680,2 ppm pada pengujian pertama dan 529,66 ppm pada pengujian duplo. Analisis probit pada pengujian duplo menghasilkan nilai LC₅₀ sebesar 198,97 ppm. Nilai LC₅₀ yang berada di bawah 1000 ppm menunjukkan bahwa ekstrak metanol batang prasman bersifat toksik terhadap larva Artemia salina dengan kategori moderat atau racun sedang. Aktivitas toksik tersebut diduga berasal dari kandungan metabolit sekunder yang terdapat dalam batang prasman seperti Flavonoid, saponin, tanin dan minyak atsirinya. Dengan demikian, ekstrak metanol batang prasman berpotensi sebagai sumber senyawa bioaktif yang dapat dikembangkan lebih lanjut dalam bidang farmasi, khususnya sebagai kandidat antikanker, antimikroba, dan bioinsektisida alami.

Penelitian ini bertujuan untuk mengkarakterisasi tingkat toksisitas ekstrak etanol 90% daun trembesi (Samanea saman) terhadap larva Artemia salina melalui penentuan nilai LC₅₀ menggunakan metode Reed-Muench dan analisis probit. Penelitian ini merupakan penelitian eksperimental dengan rancangan post-test only control group design. Larva Artemia salina digunakan sebagai organisme uji dan dipaparkan pada berbagai konsentrasi ekstrak, yaitu 10 ppm, 100 ppm, 500 ppm, 1000 ppm, 5000 ppm, dan 10.000 ppm. Parameter yang diamati adalah jumlah kematian larva setelah 24 jam perlakuan. Hasil penelitian menunjukkan bahwa persentase kematian larva meningkat seiring dengan kenaikan konsentrasi ekstrak, yang mengindikasikan adanya hubungan dosis-respons. Perhitungan nilai LC₅₀ dengan metode 823 ppm. Berdasarkan klasifikasi toksisitas Brine Shrimp Lethality Test (BSLT), nilai LC₅₀ tersebut termasuk dalam kategori toksik moderat / sedang. Hal ini menunjukkan bahwa ekstrak etanol 90% daun trembesi memiliki aktivitas toksisitas sedang terhadap larva Artemia salina. Dengan demikian, ekstrak ini berpotensi sebagai sumber senyawa bioaktif yang dapat dikembangkan lebih lanjut namun diperlukan penelitian lanjutan untuk mengidentifikasi senyawa aktif serta mengevaluasi keamanan pada sistem biologis yang lebih kompleks.