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Eukaryotic microorganisms, particularly fungi and protists, play a vital role in ecosystems, as well as in microbiology studies and biology education. The complex structure of eukaryotic cells, such as a true nucleus and membrane-bound organelles, enables these two groups to perform diverse physiological functions and adapt to their environments. This study aims to synthesize the literature on the structure and function of eukaryotic cells in fungi and protists and their implications for biology learning. The method used is a literature review with a descriptive qualitative approach through analysis of relevant scientific sources. The results of this study indicate that understanding the structure of eukaryotic cells in fungi and protists is not only important in the context of microbiology but also contributes to strengthening understanding of the concept of cell structure and function and making biology learning more contextual and meaningful.

Public space revitalization is an important strategy in urban development aimed at improving the quality of urban environments while stimulating social interaction and local economic activities. This study aims to evaluate the implementation of the Motang Rua Field revitalization program in Manggarai Regency and assess the extent to which the program achieved its intended development objectives. The study employed a qualitative descriptive approach. Data were collected through field observations, in-depth interviews with key informants including local government officials, project contractors, small business actors, and community members, as well as documentation related to the revitalization program. Data were analyzed through data reduction, data display, and conclusion drawing, supported by triangulation techniques to ensure data validity. The findings indicate that the revitalization program demonstrates a high level of relevance to community needs and aligns with the regional development vision of Ruteng Kota Molas. The implementation process shows strong coherence through effective inter-institutional coordination and consistency between planning and execution. The revitalization has also proven effective in improving public facilities, enhancing community social activities, and stimulating local economic growth through increased micro and small business activities. Furthermore, the program was implemented efficiently in terms of budget management and project timeline. The revitalization also contributes to strengthening socio-cultural values through the preservation of the historical figure Motang Rua and shows strong sustainability potential through increased public utilization of the revitalized space.

Micro, Small, and Medium Enterprises (MSMEs) play a strategic role in promoting economic growth and improving community welfare, particularly at the regional level. In Nagekeo Regency, the ikat weaving craft sector represents an important local economic potential that also carries significant cultural value. However, weaving entrepreneurs still face several challenges, including limited business capital, restricted market access, and low utilization of digital technology for product marketing. This study aims to analyze the role of the Department of Cooperatives, Industry, and Trade of Nagekeo Regency in empowering MSME actors, particularly ikat weaving groups. The research employs a qualitative approach with a descriptive research design. The study involved 18 informants consisting of local government officials, MSME actors, and institutional partners selected through purposive sampling. Data collection techniques included observation, in-depth interviews, and documentation, while data analysis was conducted through data reduction, data presentation, and conclusion drawing. The findings indicate that the department performs facilitative, educative, and technical roles in empowering weaving groups through production training, digital marketing assistance, provision of business facilities, and technical guidance on product innovation. These programs contribute to improving weavers’ skills, product quality, and market opportunities, although their implementation still faces limitations related to budget constraints and uneven program coverage.

Risky sexual behavior among adolescents is an increasingly concerning public health issue, particularly due to the lack of comprehensive sexual education in school and family environments. This community service activity aimed to improve the knowledge, awareness, and preventive attitudes of students at SMA Negeri 2 Tomohon regarding the dangers of free sex and the importance of maintaining reproductive health. The method used was educational socialization through interactive lectures, demonstrations, and open discussions involving 60 students as participants. Evaluation was conducted using pre-test and post-test instruments to measure improvements in participants' knowledge. The results showed a significant increase in knowledge, with the average pre-test score of 52.3 increasing to 80.7 in the post-test (a 54.3% improvement). A total of 91.7% of participants reached the good knowledge category after the activity, compared to only 18.3% before the activity. Participants also demonstrated more positive and critical attitudes toward the risks of free association. This activity affirms that interactive education-based socialization on reproductive health is effective in improving adolescent understanding. It is recommended that schools integrate reproductive health content regularly in counseling guidance programs to strengthen the sustainability of this activity's impact.

This study aims to determine the extent of the role of the school environment in influencing the learning motivation of class X students in Muhammadiyah Maumere High School. The school environment is an external factor that plays an important role in supporting or inhibiting the learning process of students. The factors studied in this study include the physical condition of the school (such as classrooms, libraries, laboratories, and sports facilities), social aspects (support of teachers and peers), as well as psychological aspects (comfort, appreciation, and conducive learning atmosphere). The method used in this study is a qualitative descriptive method with data collection techniques through observation, interviews, and questionnaires to students and teachers. The result of the study shows that school facilities in Muhammadiyah Maumere High School still have many limitations, including inadequate classrooms, lack of learning technology tools such as projectors, small libraries with limited collection of books, and the unavailability of special space for arts and special sport facilities. However, despite these limitations, most students still show a high level of learning motivation. This is influenced by the support of teachers and peers, involvement in extracurricular activities, family support, and personal expectations of student achievement. This study concluded that the school environment indeed plays an important role in shaping student learning motivation, but social and psychological factors also have a big influence. Therefore, it is recommended that schools make improvements and develop physical facilities and strengthen positive social climate to support the student learning process optimally. 

This study aims to analyze the potential of eupatorin found in Cat’s Whiskers (Orthosiphon stamineus) as an anticancer agent using in silico methods. Eupatorin was selected due to its promising biological activity reported in previous literature. The research employed compound structure data registered in PubChem, analyzed through PASS Online for pharmacological activity prediction, ProTox-II for toxicity evaluation, and pkCSM for ADMET parameters. The results indicated that eupatorin has a high probability of inhibiting cancer cell proliferation with significant pharmacological activity values. Toxicity predictions showed a safe profile with an LD50 supporting its potential therapeutic use. ADMET analysis demonstrated that eupatorin possesses good bioavailability, adequate absorption and distribution, and minimal metabolic interactions, supporting its efficacy as an anticancer compound. These findings suggest that eupatorin could be an important candidate for herbalbased drug development, particularly for cancer therapy, and provide opportunities for further research through in vitro and in vivo experiments to validate anticancer activity comprehensively.

Management of hazardous and toxic waste (B3) in hospitals is an important aspect of maintaining environmental health and preventing pollution risks. RSUD Jenderal Ahmad Yani Metro has implemented hazardous waste management using the reduce, reuse, recycle (3R) concept; however, its implementation has never been comprehensively evaluated. This study aimed to evaluate the management of hazardous waste using the 3R concept at RSUD Jenderal Ahmad Yani Metro based on the Regulation of the Minister of Environment and Forestry No. P.56/Menlhk-Setjen/2015 and to identify the supporting factors. This research used a qualitative design with a case study approach. The research informants consisted of 23 participants selected using purposive sampling, including the hospital director, ward heads, IPCN, sanitation officers, 3R waste management officers, and sanitation operators. Data were collected through observation, in-depth interviews, and document review using interview guidelines and checklists. Data analysis was conducted using the Miles and Huberman model, including data reduction, data display, and conclusion drawing. The results showed that the transportation, storage, and processing stages of hazardous waste management were in accordance with the Regulation of the Minister of Environment and Forestry No. P.56/Menlhk-Setjen/2015. However, the sorting and containerization processes were not fully compliant because recyclable waste was still mixed with other hazardous waste. In addition, several supporting factors were identified, including the lack of training and certification among human resources, inadequate infrastructure, and the absence of technical guidelines and standard operating procedures for the 3R waste bank. Therefore, improving human resource capacity, infrastructure, and internal hospital regulations is necessary to optimize 3R-based hazardous waste management.

This study aims to analyze the spatial pattern of poverty in Gorontalo Province in 2024 using global and local spatial autocorrelation approaches. The data used are the percentages of the poor population in each regency/municipality, obtained from the BPS. The analyses include descriptive statistical analysis, the Moran's Index test for global spatial autocorrelation, and the Local Indicators of Spatial Association (LISA) for local autocorrelation. The results show that poverty in Gorontalo Province tends to be unevenly distributed and exhibits a significant spatial pattern. The Moran's Index indicates positive spatial autocorrelation, where areas with high poverty levels tend to be adjacent to other areas with similarly high poverty levels. The LISA results identify Bone Bolango Regency as a High-Low area, meaning it has a high poverty rate but is surrounded by areas with low poverty rates. These findings highlight the importance of spatial approaches in formulating more targeted poverty alleviation policies.

This study aims to examine the forms and functions of representative speech acts in educational-themed video podcasts published on the Kampus Merdeka YouTube channel. This research is grounded in the phenomenon of social media, particularly YouTube, as a means of interactive, participatory communication and learning. In this context, the language used is key to conveying information authentically and responsibly. This study uses a qualitative descriptive method, with data collected through listening and note-taking. The research data consists of utterances containing representative speech acts, such as explaining, stating, informing, mentioning, acknowledging, giving examples, and speculating. Analysis was carried out using the matching method to identify the meaning and function of the utterances based on their context. The results show that representative speech acts play an important role in conveying information and views objectively, as well as strengthening the credibility of the source in the educational context. Through representative speech acts, the message conveyed becomes clearer, more convincing, and more relevant to the topic of discussion. These findings are expected to contribute to the development of effective communication strategies in educational digital media and serve as a reference for educational content creators in the digital era

This study analyzes pragmatic phenomena in digital communication, particularly those related to speech acts and their impact on public perception in the controversial case of an ice cube seller accused without clear basis by the authorities. This study applies a qualitative approach with a pragmatic analysis method based on discourse analysis. Data sources were obtained from online media news, social media posts, and official statements circulating in the digital public space. The analysis techniques used include identifying types of speech acts, interpreting implied meanings based on context, and analyzing public responses. The results show that statements from the authorities contain implications that trigger negative views even though they are not supported by strong evidence. This results in perlocutionary effects in the form of social stigma, reputational damage, and psychological stress for the victim. Conversely, the victim's communication strategies, which include being polite, defensive, and informative, can build a positive image and attract public sympathy. Furthermore, social media plays a crucial role in accelerating the spread of meaning and strengthening the construction of opinions through mass support and criticism. This research makes a significant contribution to enriching the study of pragmatics in the context of digital communication and emphasizes the importance of language ethics, media literacy, and pragmatic awareness in creating responsible public communication.

The Fréchet distribution is one of the commonly used Extreme Value Distributions (EVDs) in statistical modeling and heavy-tailed data analysis, where it plays an important role in describing product lifetimes as well as climatic and financial phenomena. The estimation of its two parameters, namely the shape parameter and the scale parameter, is traditionally based on the Maximum Likelihood Estimation (MLE) method. However, maximizing the likelihood function for this distribution involves numerical difficulties, which necessitates the use of numerical optimization methods. In this study, we propose the use of the Aquila Optimizer (AO), a recent metaheuristic algorithm inspired by the hunting behavior of eagles, as an efficient numerical tool for maximizing the likelihood function of the Fréchet distribution. The objective function was formulated as the negative log-likelihood function (-LogL), and the Aquila Optimizer was employed to obtain the optimal estimates of the distribution parameters. Several simulation experiments with different sample sizes were conducted to compare the performance of the proposed method with a conventional approach represented by the Nelder–Mead method, using the Mean Squared Error (MSE) criterion. The simulation results demonstrated that the Aquila Optimizer outperformed the Nelder–Mead algorithm in many cases, although the superiority was slight. The results also showed that both algorithms were consistent, as their MSE values decreased with increasing sample size. In addition, a practical application was carried out using real data, and the results of the survival function estimation indicated a good fit.

Digital transformation has reshaped the landscape of MSMEs in Indonesia, particularly those managed by Generation Z. This study aims to analyze key success factors of Generation Z MSMEs in building sustainable digital business ecosystems. The research employs a systematic literature review methodology by analyzing various academic sources, industry reports, and related publications from 2019-2024. Findings reveal five primary success factors: adaptive digital technology adoption, high digital literacy, social media utilization as marketing strategy, collaboration within digital ecosystems, and business sustainability orientation. Generation Z MSMEs demonstrate advantages in leveraging e-commerce platforms, digital payments, and data analytics for decision-making. Research implications provide strategic recommendations for MSME practitioners, policymakers, and stakeholders in developing ecosystems that support sustainable digital business growth, while emphasizing the importance of digital infrastructure and business mentoring aligned with digital native generation characteristics.

This study discusses the four pillars of digital transformation that are currently changing the business landscape in Indonesia, namely the digital ecosystem, digital twin technology, open innovation framework, and agile transformation. This study aims to identify and explain the key concepts underlying digital business innovation and its impact on business development in the modern era. The method used is a literature study with descriptive analysis of various primary and secondary sources related to digital innovation. The findings show that the application of these four pillars drives operational efficiency, increased cross-sector collaboration, accelerated product and service innovation, and increased business competitiveness. The implications of this study emphasize the importance of adopting a holistic digital strategy for companies to survive and thrive in an increasingly competitive and dynamic market. By understanding and implementing these pillars of digital transformation, Indonesian businesses can leverage the latest technologies to create innovative and sustainable business models.

This research examines the influence of the Webtoon platform on the creativits and literary marketing of Gen – Z in Indonesia, with a focus on the increasingly common trasition of digital content to physical books. The research background is grounded in the rapid growth of Webtoon as a platform for illustrated stories accessed by millions of young users via mobile devices, enabling affordable creative expression, but often encountering adaptation challenges to print formats due to differences in distribution and monetization. The primary objective is to analyze how Webtoon shapes the creative processes of young writers and their literary marketing strategies. The research method is qualitative, involving in-depth analysis of 10 popular Webtoons on the Webtoon platform that have been adapted into physical books, with emphasis on narrative elements, visuals, community interactions, and marketing patterns. Findings reveal that Webtoon fosters creativity through collaboration features and instant feedback, resulting in more dynamic original stories, while the transition to physical books enhances market visibility by 20-30% via digital promotion, although hindered by copyright complexities and production costs. The implications of this research underscore the importance of collaboration between traditional publishers and Webtoon to establish a hybrid literary ecosystem that sustainably supports Generation Z talent.

The Regional Public Library of Sidoarjo Regency plays an important role as a public facility in supporting the development of community literacy. Along with population growth and the advancement of the education sector, the library is required to provide spaces that are comfortable, safe, and inclusive for all users. However, existing conditions indicate various architectural problems that potentially hinder the optimal function of the library. This study aims to identify architectural issues at the Regional Public Library of Sidoarjo Regency, including building conditions, spatial layout, user comfort, and the availability of thematic literacy facilities and inclusive spaces. The research method employed observation and literature review. Field observation was conducted to document the actual conditions of the building and library facilities, while the literature review focused on library standards, building regulations, and relevant architectural theories. The results show that although the library building is structurally adequate, the quality of space has not fully met the required standards of comfort, safety, and functionality. The spatial arrangement remains conventional and lacks flexibility, limiting its ability to support diverse literacy activities. In addition, thematic literacy facilities and the application of inclusive design principles are still limited. This study concludes that architectural development efforts are needed, focusing on improving spatial quality, implementing adaptive spatial planning, and providing more diverse and inclusive literacy facilities. The findings are expected to serve as a reference for future development planning of the Regional Public Library of Sidoarjo Regency in order to enhance its role as an educational public space and a community literacy center.

The development of digital technology has encouraged businesses to utilize social media as an effective marketing tool, particularly in the fashion industry targeting Generation Z. In addition to digital promotional strategies, product quality is also an important factor influencing consumers’ purchase decisions. This study aims to analyze the effect of social media marketing and product quality on purchase decisions among consumers of Jims Honey Kaliwungu Branch. This research employed an explanatory type with a quantitative approach. The sample consisted of 100 respondents selected using purposive sampling. Data were collected through questionnaires and analyzed using validity testing, reliability testing, multiple linear regression, t-test, F-test, and the coefficient of determination with the assistance of SPSS. The results show that social media marketing has a positive and significant effect on purchase decisions, with a t-value of 2.293 greater than the t-table value of 1.984 and a significance value below 0.05. Product quality also has a positive and significant effect on purchase decisions, with a t-value of 6.631 greater than the t-table value of 1.984 and a significance value below 0.05. Simultaneously, social media marketing and product quality have a significant effect on purchase decisions, with an F-value of 25.994 greater than the F-table value of 3.09 and a significance value below 0.05. The coefficient of determination of 0.349 indicates that both independent variables contribute 34.9% to purchase decisions. These findings confirm that product quality has a more dominant influence than social media marketing in encouraging purchase decisions among consumers of Jims Honey Kaliwungu Branch.

Self-confidence is an important psychological aspect that affects students' academic, social, and emotional success. Students with low self-confidence often struggle with interactions, expressing opinions, and optimizing their academic potential, which can negatively impact their personal development in school. Therefore, this issue requires attention through appropriate guidance and counseling services. This study aims to review various guidance and counseling strategies to address low self-confidence in students based on a literature review of the last six years. The method used is a library study with a traditional literature review approach, analyzing 15 national and international journal articles. The results indicate that low self-confidence is influenced by internal factors (such as negative self-concept and low self-efficacy) and external factors (such as bullying experiences and lack of social support). The impacts include decreased academic performance, social barriers, and negative emotions such as anxiety. Various guidance strategies, such as individual counseling, group counseling with sociodrama, and empathetic teacher communication, have proven effective in enhancing students' self-confidence. These findings suggest that low self-confidence is not a permanent condition but can be addressed through planned and ongoing guidance interventions. .

Based on data from FAO (Food and Agriculture Organization of The United Nations), it has been predicted that the aquaculture subsector will become one of the most relied-upon resources in the future, especially in meeting food needs, fish-based nutritional needs, and empowering the global community's economy (FAO, 2016). Catfish is a type of freshwater fish that can grow and develop well because it can be cultivated in limited land areas (Marsela, 2018). The frequency of feeding is one of the important factors that need to be considered in catfish farming. To determine the effect of different feeding frequencies on the absolute weight of dumbo catfish, a study was conducted using an experimental design called the Completely Randomized Design (CRD), with 3 treatments and a total of 8 replications. The test treatments were as follows: Treatment 1 (feeding twice a day), Treatment 2 (feeding three times a day), and Treatment 3 (feeding four times a day). The results of the ANOVA test showed a significant value of 0.000 (P < 0.05), indicating a significant difference in each of the different feeding frequencies. The analysis of the Least Significant Difference (LSD) test at a 5% significance level also showed that the relationship between each treatment had a significant value of 0.000 (P < 0.05). The average absolute weight of the fish for Treatment 1 was 12.99 grams, for Treatment 2 was 15.03 grams, and for Treatment 3 was 17.03 grams. The highest growth in the absolute weight of dumbo catfish fry occurred in Treatment 3 (feeding four times a day).

Public service is a key indicator in evaluating local government performance; however, various service issues continue to generate increasing public complaints. This study aims to analyze trends in public complaints, identify the most frequently reported institutions, and examine the types of maladministration and the status of complaint resolution through the Ombudsman of the Republic of Indonesia in South Sulawesi Province during 2023–2025. This study offers novelty by providing an integrated analysis of complaint trends, distribution of reported institutions, types of maladministration, and resolution outcomes based on Ombudsman data at the regional level. This research employs a qualitative approach using document analysis, with public complaint reports as the unit of analysis. Data were analyzed through data reduction, data display, and conclusion drawing. The results indicate that local governments are the most frequently reported institutions (55.1%), with dominant maladministration types including procedural deviations and prolonged delays. Several complaints were proven to involve maladministration and were resolved through the Ombudsman’s supervisory mechanism. These findings highlight that public complaints serve as an important instrument in enhancing accountability and transparency in public service delivery. Therefore, local governments need to strengthen service standards, improve the capacity of public officials, and develop more effective complaint management systems.

Bureaucratic reform in Indonesia has encouraged the strengthening of civil service management oriented toward performance through the policy of appointing Government Employees with Work Agreements (PPPK) in order to improve the effectiveness of public organizations. This study aims to analyze the performance of PPPK in supporting the implementation of tasks at the Environmental Agency of Jambi City by using Mangkunegara’s performance theory as the analytical framework. This research employs a qualitative approach with a descriptive method. Data were collected through in-depth interviews and documentation, and then analyzed using data reduction, data display, and conclusion drawing techniques. The results show that the performance of PPPK is generally in the good category and contributes positively to organizational effectiveness. This is reflected in the aspects of work quality, work quantity, and responsibility as explained in Mangkunegara’s performance theory. The alignment between job placement and competence, increased work motivation due to employment status certainty, and organizational support through training and coaching are the main factors supporting performance optimization. However, there are still constraints, particularly the suboptimal understanding of main duties and functions, thus requiring strengthened organizational communication and continuous capacity development. This study confirms that competency-based management and systematic development of PPPK play an important role in improving the effectiveness of task implementation in the public sector.