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This study aims to comparatively analyze the effects of two types of inhalation anesthetics, ether and chloroform, on Wistar rats, focusing on three main parameters: induction time, duration of anesthesia, and side effects. Selecting the appropriate anesthetic method is crucial in animal studies to ensure animal welfare and the validity of the results. Twenty male Wistar rats, with an average body weight of 200–250 grams and 10–12 weeks of age, were randomly divided into two treatment groups of 10 rats each. The first group was anesthetized using ether, while the second group was anesthetized using chloroform, with doses adjusted to achieve adequate levels of anesthesia. Measurements were made of the induction time, which is the interval from the start of anesthetic exposure until the loss of the pedal reflex; the duration of anesthesia, which is the length of time until the rat regains consciousness; and clinical observations of any side effects that appear, both during and after anesthesia. The results showed that the ether group had a relatively longer induction time than the chloroform group. However, ether provided a longer and more stable duration of anesthesia, with a better recovery rate. In contrast, chloroform produced a shorter induction time but was accompanied by a shorter duration of anesthesia and a higher risk of side effects, particularly liver dysfunction and respiratory depression. These findings reinforce previous reports that chloroform has significant potential toxicity, and its use in animal research should be limited. Based on these results, ether is recommended as a relatively safe inhalation anesthetic option for laboratory procedures in Wistar rats, although its use still requires strict supervision and appropriate handling procedures to ensure the safety of both researchers and experimental animals.

This research explores the engineering and performance evaluation of 17-4 PH stainless steel as a potential material for turbine blades in geothermal power plants (PLTP). To promote renewable energy innovation in industrial engineering, this study focuses on improving material reliability through microstructural optimization and mechanical property control. The material was produced using the investment casting method at PT SPVMB and then subjected to four heat treatment variations: H900, H1025, AVG (average), and as-cast conditions, with reference to ASTM A747 standards. Mechanical and corrosion characterization were performed through hardness and tensile tests, electrochemical corrosion analysis using geothermal water from the Dieng PLTP, and microstructural observation using an optical microscope. The results showed that the H900 condition had the highest hardness and yield strength (48.46 HRC and 939.25 MPa), but its corrosion rate was relatively high. In contrast, the H1025 heat treatment provides balanced mechanical strength (43.88 HRC and 860.91 MPa) with the lowest corrosion rate (0.027 mm/year), supported by a uniform tempered martensite structure. These findings indicate that heat treatment optimization significantly improves the suitability of 17-4 PH stainless steel for sustainable geothermal applications. The H1025 condition meets all the requirements for geothermal turbine blades, including hardness, strength, and corrosion resistance, potentially extending component life and reducing maintenance costs. Furthermore, the results of this study strengthen the agenda for developing durable, environmentally friendly materials to support renewable energy systems. This study also provides practical insights for industry in selecting the optimal heat treatment that combines mechanical performance and corrosion resistance in extreme geothermal environments.

Cough and flu are common health problems experienced by the public, with high prevalence across various age groups. The widespread practice of self-medication in the community highlighted the need for proper education regarding the rational and appropriate selection of medications. This educational activity was conducted at two strategic locations: the Car Free Day (CFD) in Solo and Taman Jaya Wijaya in Mojosongo. The method involved direct socialization, distribution of educational leaflets, and interactive question-and-answer sessions. The education focused on the differences between dry and productive coughs, appropriate medication choices, flu medicine content, and the use of natural remedies such as herbal treatments. A total of 41 participants from diverse backgrounds joined the activity enthusiastically. The results showed an increase in public understanding of cough classifications, medicine components, and the importance of reading drug labels before purchase. The sessions also emphasized the need to match medications with symptoms and advised consulting healthcare professionals if symptoms did not improve within three days. Positive responses from participants indicated that direct education effectively raised awareness and encouraged more rational and safe self-medication practices.

The presumption of innocence is a fundamental principle in the criminal justice system that serves to protect the rights of the accused from the risk of unfair punishment. This principle states that a person is presumed innocent until proven legally and convincingly guilty of committing a crime before a court. This research uses an empirical juridical method, namely a legal approach that examines how positive law, especially unwritten law, is applied in society. In this context, the research highlights the implementation of the presumption of innocence in criminal justice practices in Indonesia. The application of the presumption of innocence is very important for the judicial process to run fairly, directed, and achieve the main objectives of criminal justice, namely upholding justice, legal certainty, and legal order. The relationship between this principle and human rights is very close, because with this principle, suspects and defendants are guaranteed to obtain legal protection during the legal process. Rights such as not being treated as guilty before a court decision, the right to defense, and the right to humane treatment are part of this principle. Enforcing the presumption of innocence is not only the responsibility of law enforcement officers such as the police, prosecutors, and judges, but also all elements of society. Therefore, it is crucial for every citizen to understand and respect this principle in their social lives, especially in responding to ongoing legal cases. As a concrete implementation, law enforcement must implement policies that protect the public and maintain a sense of security, for example by increasing surveillance in crime-prone areas. This way, the law can be enforced fairly, and public trust in the criminal justice system will increase. The presumption of innocence is a crucial foundation for the creation of humane and fair trials in Indonesia.

Hashimoto's thyroiditis (HT) is an autoimmune disorder that leads to thyroid dysfunction and chronic inflammation, often resulting in hypothyroidism. The exact mechanisms driving HT are not fully understood, but recent research has highlighted the potential role of various molecular markers in the disease’s pathogenesis. This study investigated the roles of miRNA-375, miRNA-451, and interleukin-6 (IL-6) in HT. Serum samples were collected from 100 HT patients and 100 healthy controls, and their expression levels were analyzed using quantitative polymerase chain reaction (qPCR) and enzyme-linked immunosorbent assay (ELISA). The results revealed significant alterations in the levels of these molecules. miRNA-375 was significantly elevated in HT patients (5.85 ± 1.30 vs. 1.0, p < 0.001), while miRNA-451 was markedly reduced (1.43 ± 0.22 vs. 10.91 ± 2.44, p < 0.001). Additionally, IL-6 levels were found to be significantly higher in HT patients (115.79 ± 12.62 vs. 21.34 ± 4.03 pg/ml, p < 0.001). These findings suggest that these molecules play critical roles in the immunopathogenesis of HT. Receiver operating characteristic (ROC) analysis further confirmed that miRNA-375, miRNA-451, and IL-6 are excellent diagnostic markers, with area under the curve (AUC) values ranging from 0.956 to 1.000. miRNA-375 showed 96% sensitivity and specificity at a cutoff >1.66-fold, while miRNA-451 demonstrated 100% sensitivity and specificity at a cutoff <3.48-fold. IL-6 also exhibited perfect diagnostic accuracy with 100% sensitivity and specificity at a cutoff >53.73 pg/ml. No significant variations were observed across demographic or treatment subgroups, supporting the stability of these biomarkers in clinical applications. These findings suggest that miRNA-375, miRNA-451, and IL-6 are dysregulated in HT and could serve as reliable biomarkers for diagnosis and potential therapeutic targets, offering new strategies for the management of Hashimoto’s thyroiditis.

This study was conducted to analyze the effect of rainwater on the survival of koi fry. The background of this study is based on the increasing demand for koi fish which encourages breeders to improve the quality and quantity of fry production. However, the success of seeding is influenced by several factors, especially air quality such as temperature and pH. Rainwater is known to have less stable quality, especially due to its low pH value, so it is necessary to study its impact on koi fry. This study was conducted on November 25–28, 2024 in the Bossbaster experimental pond, Legok Nyenang Village, Kadudampit District, Sukabumi Regency. The media used were 15 plastic gallons, each containing 5 liters of air and 40 newly hatched koi fry. The experimental design used a Completely Randomized Design (CRD) with 5 treatment levels and 3 replications. Treatments included A (100% rainwater), B (75% rainwater + 25% well water), C (50% rainwater + 50% well water), D (25% rainwater + 75% well water), and E (100% well water). The results of the ANOVA test showed that there was no significant difference (P>0.05) in the survival value of koi fish fry in the five treatments. Thus, it can be concluded that the use of rainwater does not have a significant effect on the survival of koi fish fry. In addition, the results of water quality observations showed that all parameters were still within the standard quality range, except for the temperature which was slightly below the standard.

Panoramic radiography is an imaging technique that plays a crucial role in dental practice, particularly in supporting the diagnosis and treatment planning process. Image quality that does not meet diagnostic standards can lead to the need for repeat imaging. The high rate of repeat panoramic imaging at the Radiology Unit of Dr. Suhardi Hardjolukito Air Force Base Hospital (RSPAU) is an indicator of the need for further study of the causal factors. The purpose of this study was to identify the causes of repeat panoramic radiography examinations, calculate the percentage contribution of each factor, assess radiographers' understanding of optimal image quality criteria, and explore appropriate strategies to reduce the frequency of repeat examinations. This study used a mixed methods approach with quantitative and qualitative descriptive approaches. Data were collected through observation, interviews, questionnaires, and documentation during August–October 2024 at the Radiology Unit of Dr. Suhardi Hardjolukito Air Force Base Hospital. The sample included 15 radiographers for the questionnaire and 3 radiographers for the interviews. An analysis of 916 panoramic radiography examinations revealed 57 repeat cases, with a rate of 6.2%. The primary factors causing repeats were machine error (57.8%), followed by positioning errors (24.5%), artifacts (10.5%), human error (5.3%), and patient movement (1.8%). Radiographers' understanding of the criteria for good panoramic images was high, with 66.7% answering all questions correctly. The primary effort implemented to reduce repeat rates is the implementation of routine quality control of equipment. The high repeat rate, exceeding national tolerance limits, indicates the need for technical and procedural improvements. Routine quality control and material refresher courses are needed to reduce repeat rates and improve patient safety.  

This study aimed to evaluate the effect of post-larvae (PL) 12 density of whiteleg shrimp (Litopenaeus vannamei) on survival during transportation using a closed wet system. The study was conducted experimentally using four different density treatments: 500, 750, 1000, and 1250 shrimp per liter of water. The purpose of these density variations was to identify the optimal density level that still ensured a high survival rate during transportation. The results showed that shrimp seed density significantly influenced survival. A density of 500 shrimp/liter resulted in the highest survival rate of 99.6%, while a density of 1250 shrimp/liter showed the lowest survival rate of 99.30%. Although the differences in numbers appear small, the ANOVA statistical test showed that the differences between the treatments were significant. These results were reinforced by a further LSD (Least Significant Difference) test, which confirmed significant differences between treatment groups. In addition, water quality parameters such as dissolved oxygen, temperature, and pH were also observed during transportation. Measurement results showed that dissolved oxygen and temperature changed significantly with increasing seed density. However, pH did not show any significant differences between treatments. Overall, the results of this study indicate that shrimp seed density and water quality parameters are important factors to consider in the shrimp fry transportation system. Appropriate density management can minimize stress and mortality, and ensure seed survival during the distribution process.  

Arginine deiminase (ADI) is a promising enzyme with significant therapeutic potential, particularly for its anticancer effects through the depletion of arginine in cancer cells that are auxotrophs. In this study, we aimed to optimize the production of ADI using clinical Escherichia coli isolates and to evaluate its antioxidant activity. A total of 25 E. coli isolates were obtained from 45 hospital samples collected in Wasit Province, Iraq. Optimization of ADI production was performed by systematically testing various factors including culture media, pH, carbon and nitrogen sources, incubation temperature, and time. The antioxidant activity was assessed using the DPPH radical scavenging assay. The highest ADI production was achieved using a modified M9 medium supplemented with 1% w/v sucrose as the carbon source and 5% w/v yeast extract as the nitrogen source. The optimal enzyme activity of 1.6 U/mg protein was observed at pH 7.0, 37°C, and after 24 hours of incubation. The crude ADI extract exhibited high antioxidant activity, with 79.28 ± 1.06% DPPH scavenging at 200 µg/mL, comparable to ascorbic acid, which showed 86.11 ± 1.45% DPPH scavenging. The study successfully optimized the conditions for enhanced ADI production based on clinical E. coli isolates, demonstrating its potential as both an anticancer enzyme and an antioxidant. The dual therapeutic potential of ADI warrants further research, including clinical trials, to explore its application in cancer therapy and as an antioxidant in medical treatments, offering promising avenues for future drug development and improved therapeutic strategies, particularly for targeting cancer and oxidative stress-related diseases.

Pain is an unpleasant sensation that can interfere with an individual's quality of life and requires effective management. Synthetic analgesics such as ketorolac, codeine, etoricoxib, and celecoxib are commonly used to relieve pain, but long-term use can cause adverse side effects, including gastrointestinal disturbances, dependence, and cardiovascular risks. Therefore, it is important to find safer, natural alternatives. One promising alternative is the extract of the dragon’s tail leaf (Rhaphidophora pinnata), a plant traditionally used in Indonesian medicine as a pain reliever. This study aims to evaluate and compare the analgesic effects of Rhaphidophora pinnata leaf extract with several synthetic drugs using the writhing test on male mice (Mus musculus). The mice were induced with pain by injecting a 0.5% acetic acid solution. The treatments included ketorolac, codeine, etoricoxib, celecoxib, and Rhaphidophora pinnata leaf extract. The results showed that all treatments, including both synthetic drugs and Rhaphidophora pinnata leaf extract, produced significant analgesic effects compared to the negative control (Na CMC 0.5%). Ketorolac showed the highest effectiveness, followed by codeine, etoricoxib, and celecoxib. Although the Rhaphidophora pinnata leaf extract showed potential as a natural analgesic, its effectiveness varied among individual test animals. Some mice showed a better response to the leaf extract, but overall, the analgesic effect was still lower than that of synthetic drugs. These findings support the use of medicinal plants as a safer alternative to synthetic analgesic drugs. Moreover, this study provides a foundation for further research aimed at isolating active compounds from Rhaphidophora pinnata leaf extract to develop more effective and safer pain-relieving medications.

Primary dysmenorrhea is menstrual pain without any organic abnormalities, commonly experienced by adolescent girls, which affects daily activities and quality of life. Non-pharmacological treatments such as warm compresses and lavender aromatherapy are considered effective and safe alternatives. This study aimed to analyze the difference in effectiveness between the application of warm compresses and lavender aromatherapy in reducing dysmenorrhea pain among female students at SMA Ummu Rahmah Patumbak. The study used a quasi-experimental design with a pretest-posttest two-group comparison approach. A total of 20 respondents were divided into two treatment groups. Pain levels were measured using the Numeric Rating Scale, and data were analyzed using the Wilcoxon and Mann-Whitney tests. The results showed a significant reduction in pain in both groups (p<0.05), with the average pain score in the warm compress group decreasing from 5.50 to 0.55, and in the lavender aromatherapy group from 5.30 to 0.65. In conclusion, both interventions effectively reduced dysmenorrhea pain, with warm compresses showing greater effectiveness.

This study aims to determine the effect of adding EM4 probiotics in various doses to commercial feed on the growth of jatimbulan tilapia (Oreochromis niloticus L). Probiotics are known to play a role in increasing digestive efficiency, nutrient absorption, and maintaining the health of the fish's digestive tract, so they are expected to support optimal growth. The research method used was a Completely Randomized Design (CRD) with four treatments, namely: P0 (control/without probiotics), P1 (5 ml EM4/kg feed), P2 (7 ml EM4/kg feed), and P3 (11 ml EM4/kg feed), each with four replications. The parameters observed were the growth in length and weight of jatimbulan tilapia during the maintenance period. The results showed that the administration of EM4 probiotics at certain doses was able to provide a significant effect on the growth of tilapia compared to the control treatment. Treatment P2 (7 ml/kg feed) provided the best growth results compared to other doses, both in terms of increasing length and body weight. Meanwhile, administering probiotic doses that are too low (P1) or too high (P3) tends to be less effective in supporting optimal growth. This is thought to be due to the disruption of the microbial balance in the digestive tract when the probiotic dosage is incorrect. This research provides useful information for Jatimbulan tilapia cultivation practices, particularly regarding the use of probiotics to increase growth efficiency. Using the correct probiotic dosage can be a strategy to increase the productivity and sustainability of freshwater fish farming.  

Rainwater is often considered to have a negative impact on the survival of fish fry, mainly due to its slightly acidic pH. However, this study aims to investigate the effect of rainwater on the survival rate of comet fish fry (Carassius auratus). The research was conducted at the Bossbaster experimental pond, Legok Nyenang Village, Kadudampit District, Sukabumi Regency, over a period of three days. The fish fry used were one day old after hatching, with a size of approximately 5 mm. The study utilized 15 containers with a water volume of 10 liters per gallon and 15 aeration devices to maintain water circulation. Five treatments were tested: A (100% rainwater), B (75% rainwater), C (50% rainwater), D (25% rainwater), and E (0% rainwater). The research design followed a Randomized Complete Block Design (RCBD) with three replications for each treatment. The data were analyzed using the Median Test and Regression Test to maximize the obtained results. The results showed that rainwater did not significantly affect the survival rate of comet fish fry. The survival rate of the fry in all treatments ranged from 98.33% to 99.17%. This indicates that although rainwater has a lower pH, it does not significantly affect the survival of comet fish fry. Based on these findings, it can be concluded that the rainwater taken from the research site did not negatively impact the survival rate of comet fish fry. The quality of the rainwater used in this study is safe and can be used as a medium for raising fish fry without causing a decrease in their survival rate. This study provides valuable information on the use of rainwater in fish farming, especially for comet fish fry.

The maternal mortality rate remains a significant global health challenge, particularly in developing countries. Timely detection of obstetric emergencies such as preeclampsia, sepsis, and postpartum hemorrhage is crucial to reducing delays in treatment. This literature review aims to analyze the implementation and effectiveness of the Modified Early Obstetric Warning Score (MEOWS) in primary healthcare settings. The review included 22 articles published between 2016 and 2023, identified through databases such as Scopus, PubMed, and ScienceDirect. Findings indicate that MEOWS is effective in detecting early signs of maternal deterioration, improving clinical decision-making, and preventing severe complications. However, challenges remain in adopting MEOWS in primary care due to limited training, lack of standardized protocols, and inadequate resources. The results highlight the need for capacity building, policy support, and contextual adaptation of MEOWS to optimize its use in community-based maternal care. This review suggests that the integration of MEOWS can strengthen obstetric triage systems and contribute to lowering maternal mortality rates in low-resource settings.

Thalassemia is a genetic disorder characterized by impaired hemoglobin synthesis. This disease is caused by mutations in the globin gene, leading to disrupted production of globin chains. As a result, the red blood cells produced are dysfunctional and have a shorter lifespan, causing anemia. This condition requires proper medical management, including blood transfusions and other treatments. One way to detect and monitor the progression of thalassemia is by using biochemical markers that can identify changes in the patient’s body. Therefore, the aim of this systematic literature review is to identify biochemical markers that can be used for the diagnosis and monitoring of thalassemia. The literature used in this study includes articles on human thalassemia research published in the last 10 years. Literature searches were conducted in several academic databases using relevant keywords such as “biochemical markers for thalassemia,” “diagnosis of thalassemia,” and “thalassemia monitoring.” Based on the search results, several biochemical markers related to thalassemia were identified, including hepcidin, ferritin, and lipid profile. Ferritin plays a role in monitoring iron levels, which are often elevated in thalassemia patients, while hepcidin regulates iron homeostasis in the body. Additionally, other components involved in thalassemia diagnosis and monitoring include Mean Corpuscular Volume (MCV), Mean Corpuscular Hemoglobin (MCH), Mean Corpuscular Hemoglobin Concentration (MCHC), and hemoglobin levels. The findings of this systematic literature review are expected to provide a comprehensive overview of biochemical markers that can be used in the diagnosis and monitoring of thalassemia. By identifying relevant markers, it is hoped that more accurate and effective diagnostic methods will be developed in the future, leading to better monitoring of thalassemia patients.

The differences in the age limits of children as stipulated in several Indonesian laws and regulations—such as the Juvenile Criminal Justice System Law (UU Sistem Peradilan Pidana Anak/SPPA), the Child Protection Law (UU Perlindungan Anak), and the Human Rights Law (UU HAM)—have created significant legal uncertainty. These inconsistencies pose serious implications for the protection of children's rights, particularly for those who are entangled in legal conflicts. One of the most critical impacts is the potential for injustice in the application of restorative justice and diversion programs, which should be guided by the principle of the best interests of the child. Discrepancies in age definitions may also result in unequal treatment in legal proceedings and hinder access to appropriate legal remedies and rehabilitation services. For instance, a child considered underage by one law may be treated as an adult under another, leading to inconsistent judicial decisions, discrimination, and psychological harm. Furthermore, this lack of regulatory harmony undermines the credibility and consistency of the justice system in handling cases involving children. The principle of legal certainty, which is fundamental in any just legal system, cannot be upheld if such foundational definitions remain fragmented. Therefore, there is an urgent need for harmonization and alignment of all regulations related to the legal age definition of a child within the Indonesian national legal framework. Establishing a uniform age standard is essential to ensure fair treatment, protect children's rights comprehensively, prevent discriminatory practices, and improve the effectiveness of restorative justice, rehabilitation, and child protection mechanisms in Indonesia.

The low scientific literacy of elementary school students, influenced by the use of conventional learning methods and lack of interactivity, encourages the need for new innovations based on digital technology. This study aims to test the optimization of the use of digital technology as a strategy to improve scientific literacy through a quasi-experimental method with a pretest-posttest control group design. The research subjects consisted of 60 fifth-grade students from one elementary school, namely SDN Pondok Labu 03, taken from 2 classes, each consisting of 30 students (30 students in the experimental group and 30 students in the control group). The experimental group was given a digital technology-based science learning treatment, namely a virtual laboratory (Labster), and an educational application (Quizizz), for 8 weeks, while the control group used conventional methods. The research instruments included a scientific literacy test (HOTS questions) and student activity observation sheets. Data were analyzed quantitatively using t-test and ANOVA to compare the increase in scientific literacy scores between the two groups. The results showed that the experimental group experienced a significant increase in scientific literacy scores (N-Gain 0.72) compared to the control group (N-Gain 0.32). The research conclusion proves that the systematic integration of digital technology is effective in improving the scientific literacy of elementary school students at Pondok Labu 03.

A 23-month-old female patient presented with red, itchy bumps, accompanied by intense itching, which caused the skin to peel from scratching. These symptoms began less than a month before the visit and worsened one week before seeking treatment. The fluid-filled bumps burst due to scratching, causing sores and peeling skin. The itching was constant and tended to worsen at night. The use of moisturizer by the patient's mother provided little relief. The onset of these symptoms coincided with a visit from the patient's mother's younger brother, a student at the Islamic boarding school (Santri), who was staying at the patient's home during the holidays. Less than a month later, similar symptoms began to be felt by the patient, her siblings, and both parents. The diagnosis was based on primary data obtained through a history taking with the patient's mother, a direct physical examination during a home visit, and completion of the family file and the patient's medical records. The patient, suspected of having scabies, was treated with 5% permethrin cream, applied as directed, and cetirizine to reduce the itching. Case assessments are conducted holistically, assessing the initial condition, process, and final outcome of each visit both quantitatively and qualitatively. Interventions include not only treatment but also comprehensive family education. This education includes an explanation of the etiology and transmission of scabies, the importance of maintaining personal and environmental hygiene, and potential complications such as secondary skin infections due to scratching. The goal of this education is to improve patient and family compliance with therapy, prevent reinfection, and break the chain of disease transmission in the home environment. This approach is expected to not only reassure the patient but also raise family awareness of the importance of clean and healthy living practices as a long-term preventative measure.

This study aims to extract and purify secondary metabolite compounds from Coffea arabica leaves using various separation techniques, such as maceration, column chromatography, thin layer chromatography (KLT), and preparatory KLT, and followed by flavonoid content analysis using UV-Vis spectrophotometry. The process begins with drying the arabica coffee leaves, then extraction is carried out using 96% ethanol for three days. After that, the solvent is evaporated with a rotary evaporator at 60°C to obtain a viscous extract. The extracts were further separated using column chromatography with a mixed solvent of n-hexane: ethyl acetate (3:1), resulting in 12 fractions that showed color differences, which was an indication of the diversity of the compounds in the extract. Advanced identification was carried out with KLT using methanol solvents: chloroform (9:1) at UV wavelengths of 254 nm and 366 nm, indicating the presence of compounds with medium to high polarity. Furthermore, the isolated compounds were further separated using the preparative KLT (KLTP) method. To determine the flavonoid content, UV-Vis spectrophotometry was performed at a wavelength of 431 nm, using quercetin as standard. The results of the analysis showed that Arabica coffee leaf extract had a yield of 7.35% and a total flavonoid content of 19.0605 mgQE/g. These findings show that arabica coffee leaves are a source of flavonoid compounds that have the potential for the development of herbal or phytopharmaceutical products. The flavonoid content found may provide health benefits, particularly in increasing antioxidant activity, which can be used in the treatment of oxidative stress-related diseases. The results of this study also open up opportunities for the development of plant-based products, especially Arabica coffee, in the pharmaceutical and cosmetic industries as an efficacious natural active ingredient.

This study aims to examine the mediating role of Organizational Citizenship Behavior (OCB) in the relationship between Perceived Organizational Support (POS), Perceived Organizational Commitment (POC), and employee performance in a state-owned enterprise in Indonesia. In the context of growing public expectations for improved service quality, organizations are challenged to enhance employee performance not only in formal roles but also in discretionary behaviors. Based on Social Exchange Theory (SET), this research proposes that POS and POC can affect performance both directly and indirectly through OCB. Data were collected through a census of 138 permanent employees at PT Jasa Raharja, East Java Branch. The analysis employed Partial Least Squares Structural Equation Modeling (PLS-SEM) to test the hypothesized relationships. The findings show that POS has a significant positive influence on both OCB and employee performance. On the other hand, POC significantly influences OCB but does not have a direct impact on performance. The results also reveal that OCB plays a partial mediating role in the relationship between POS and performance, while it acts as a full mediator in the relationship between POC and performance. These findings emphasize the importance of fostering OCB within the organization to optimize the impact of support and commitment on employee performance. Encouraging voluntary, extra-role behaviors can bridge the gap between how employees perceive organizational treatment and how they perform. The study suggests practical implications for human resource management in public sector institutions. Policies should focus on building a supportive and engaging work climate that enhances affective commitment and acknowledges employee contributions beyond formal duties. Doing so will not only improve individual performance but also contribute to broader organizational effectiveness in a competitive and service-oriented environment.