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Ciplukan leaves, kamilen flowers, and stevia leaves are widely used herbal plants known for their pharmacological activities, which are mainly attributed to the presence of secondary metabolite compounds. These compounds play an important role in providing therapeutic effects such as antioxidant, anti-inflammatory, and antimicrobial properties. One approach to enhancing the effectiveness of these plants is by combining them into a single preparation. This study aims to identify the content of secondary metabolite compounds in a combined infusion (infusa) of ciplukan leaves, kamilen flowers, and stevia leaves. The research method used is a laboratory experimental approach, which includes plant determination, preparation of infusion, and phytochemical screening. The infusion process is carried out to extract active compounds using water as a solvent under controlled conditions. The phytochemical screening is conducted to qualitatively detect the presence of various bioactive compounds. The results of the screening on three formulations (F1, F2, and F3) show that all infusa preparations contain several important secondary metabolites, including alkaloids, flavonoids, saponins, tannins, and terpenoids. These findings indicate that the combination of the three herbal plants has the potential to provide enhanced pharmacological effects due to the diversity of active compounds present in the formulation.

Secondary metabolites are compounds produced by plants, microorganisms and animals through the process of biosynthesis or derivatives from primary metabolites. The maceration method is a simple way to separate bioactive compounds from plant material. Guava leaves contain secondary metabolite compounds which have medicinal effects such as antibacterial, antioxidant and anti-inflammatory. This simplicia leaf is soaked in 96% ethanol (using the maceration method) for 24 hours and concentrated using a rotary evaporator. Alkaloid, flavonoid and saponin tests show that guava leaves contain these three compounds. These compounds have potential for development in the pharmaceutical field.

As a tropical country, Indonesia has many types of biodiversity, including plants that are spread throughout Indonesia, approximately as many as 40,000 species with 7,500 species of which can be used as medicinal plants which are applied for medical needs and biological and chemical research. One of the topics discussed in this journal is the gotu kola leaf, known as the Latin name (centella asiatica). This plant contains tannins, flavonoids, vitamin A and vitamin C with various properties including accelerating the wound healing process, other benefits such as improving brain and nerve function. It should be noted that this efficacy is due to the presence of secondary metabolites in the gotu kola leaf and to find out, alkaloid, flavonoid, and saponin tests were carried out using dried gotu kola leaves and then divided into two bottles of 200 ml of methanol and N-hexane solution and then macerated for 3 days. . After the maceration process is complete, a thick extract of gotu kola leaves is produced and then continued with testing for alkaloids, flavonoids, and saponins which will later get results that aim to be the first step to determine secondary metabolite compounds and be useful as information on the utilization of the efficacy of gotu kola leaves as well as research in biology and chemistry.

The study aimed to investigate the alkaloid content in cinnamon (Cinnamomum burmannii) powder using the Soxhlet extraction method. Alkaloids are organic chemical compounds widely found in nature as secondary metabolites in both plants and animals. They possess a cyclic structure containing one or more basic nitrogen atoms, conferring unique basic properties. The research was conducted to provide a deeper understanding of the potential utilization of plant-derived alkaloids, particularly from cinnamon, in drug development. The study sought to determine the color of the precipitate formed in the extraction solution after the addition of Mayer's reagent and to investigate the effect of the number of Soxhlet extraction cycles on the color of the ethanol used in the research with cinnamon samples. The findings from this study contribute to the exploration of the alkaloid content in cinnamon and its potential applications in pharmaceutical development.

The part of the basil plant is the leaves which have various types of benefits if studied further. With the introduction of secondary metabolites, it can be said that basil leaves contain eugenol compounds. The eugenol compound can be used as a reducing agent in the synthesis of TiO2 nanoparticles. The precursor used in the synthesis of TiO2 is titanium isopropoxide which has an oxidation state of +4. By utilizing the green synthesis method, TiO2 was reacted with basil leaf extract and then characterized using XRD and FTIR. The resulting particle size is 10.86 nm. And FTIR shows a wave number of 874.59 which represents the Ti-O functional group. Photocatalytic activity can take advantage of the synthesis of TiO2 nanoparticles by placing the nanoparticles in a methylene blue solution which has a concentration of 20 ppm and varies based on contact time and adsorbent mass. Photocatalytic activity testing obtained a degradation percentage of 79%-84% which varied based on adsorbant mass and contact time. Varying the adsorbant mass and time results in a degradation percentage that increases with each additional mass but will not increase again if it has reached the optimum phase and can reduce the degradation percentage.

Transparent soap is a variation of solid bar soap that not only functions to clean dirt but also has a scrubbing function. Transparent solid soap is one of the soap innovations that has become an attractive soap. Saffron also has antibacterial properties due to the presence of flavonoids, tannins and saponins. Flavonoids are compounds that can have anti-inflammatory, anticancer and antioxidant effects, because they can inhibit the formation of free radicals. It has been used traditionally to treat various ailments including depression, cardiovascular disease, menstrual disorders, asthma, insomnia, digestive ailments, bone pain and several other ailments. Saffron pistil juice is made by dissolving in CO₂-free distilled water. Phytochemical screening was carried out on saffron pistil extract. Saffron pistil juice is made in various soap formulas, namely formula I, formula II and formula III. The positive control used was chloramphenicol antibiotic and the negative control was DMSO. There were several tests carried out on Saffron pistil extract in addition to phytochemical screening, namely antibacterial testing on transparent soap with Saffron pistil extract as a soap additive. The results of phytochemical screening showed that the pistil of saffron (Crocus sativus L) contained secondary metabolites, namely: flavonoids, alkaloids, tannins, and saponins. And for the results of the antibacterial activity research also showed that the Saffron pistil extract against transparent soap which can be used as a soap additive, the antibacterial results had a strong inhibition at the average value of formula III which was 20.5 and formula II was 18.4 mm and formula I 16.1 mm, against Staphylococcus aureus bacteria.

Acne is a disease on the surface of the skin of the face, neck, chest, and back that appears when the oil glands in the skin are too active so that the skin pores will be clogged by excessive fat deposits. Guava leaves contain secondary metabolites, consisting of tannins, alkaloids, flavonoids, saponins. This study aims to identify the content of secondary metabolites in guava leaf extract (Psidium guajava L.), test the antibacterial activity of ethanol extract of guava leaves (Psidium guajava L.) against the growth of Propionibacterium acnes and Staphylococcus aureus bacteria, and analyze the comparison of the effectiveness of guava leaf extract (Psidium guajava L.) against the growth of the two test bacteria. This type of research is laboratory experimental. The methods used for this study: preparation of materials, extraction of samples by maceration method, phytochemical screening test, testing antibacterial activity using the pitting method and analyzing data. The screening test results of guava leaf extract (Psidium guajava L.) showed the presence of alkaloid, flavonoid, tannin and saponin compounds. The results of the inhibition test on guava leaf extract with a high concentration of 115% have the largest diameter of 17.5 mm against Staphylococcus aureus compared to Propionibacterium acnes bacteria with an inhibition diameter of 16 mm. The results of the Mann-Whitney statistical test showed a p-value of 0.077 so it can be concluded that there is no comparison of the effectiveness of guava leaf extract (Psidium guajava L.) against the growth of Propionibacterium acnes and Staphylococcus aures bacteria.

Medicinal plants are plants with positive pharmacological effects on the human body and are usually grown on a home or communal scale. These medicinal plants can then be used as traditional medicine which can be made easily. The plants chosen are usually plants that can be used to treat simple health problems such as flu and coughs. Medicinal plants can increase the body's immunity because they can prevent disease through the secondary metabolites they contain. In this service activity, two activities were carried out separately, namely socialization and planting medicinal plants with residents. The outreach was carried out to provide information to the residents of Medan Helvetia District regarding medicinal plants so that these medicinal plants can be useful after being planted. Planting is also carried out to increase awareness regarding land use, as well as being a starting point for residents in cultivating medicinal plants. This socialization on the use of family medicinal plants aims to provide information to the people of Kexamatan Medan Helvetia in using medicinal plants. It is hoped that this community service activity will help increase public knowledge about the importance of planting and utilizing medicinal plants to create a healthy society.

Diuretics act on the kidneys to increase the excretion of water and sodium chloride. The working principle of diuretics in general is to reduce electrolyte reabsorption by the renal tubules, where an increase in electrolyte excretion will be accompanied by an increase in water excretion which is needed to achieve osmotic balance. Bangun-Bangun leaf contains polyphenolic compounds, saponins, glycosides, flavonoids, and essential oils. Bangun-Bangun leaves types of flavonoids, namely quercetin, apigenin, luteolin, salvigenin, and genkwanin. Apigenin is known as a compound that functions as a diuretic. IDBB was obtained from the infusion technique with distilled water and then the infusion was used on 25 diuretic rats which were divided into 5 groups. Group I (negative) Na-CMC 0.5%, group II, III, IV, IDBB doses of 10, 20, 40%, and group V (positive) Furosemide 3.6 mg/kgBW were given orally. Rats were given aquadest orally at a dose of 15 ml/kgBW. Mice were placed in metabolic cages, then a physical examination of urine was carried out every 1 hour for 6 hours. The results showed that the IDK with the best dose was 40% which gave an increase in urine volume, had an effect on urine pH, had an effect on urine specific gravity, had an effect on urine color, and had an effect on urine clarity.    

Durian leaves have secondary metabolites, namely flavonoids. Flavonoids have shown a wide range of bioactivity, one of which is an antipyretic effect which can be used as a febrifuge. This study aims to examine the antipyretic activity of the ethanol extract of durian leaves (Durio zibethinus L.) against male white rats (Rattus norvegicus) induced by DPT-HB-Hib vaccine. This study uses an experimental method. In this study, durian leaf extract was processed by maceration using 96% ethanol, then treated with secondary metabolites of simplicia and its ethanol extract. To pay for the antipyretic activity of durian leaves (Durio zibethinus L.) the test animals used were 25 and divided into 5 groups, namely negative control (CMC 0.5%), positive control (Paracetamol 1%) and the test dose group, namely the administration of leaf extract durian 200 mg/kg, 300 mg/kg and 400 mg/kg. Each rat was induced intramuscularly with the DPT-HB-Hib vaccine. Temperature measurements were carried out rectally with initial temperature measurements at 5 minute intervals 3 times, 1 hour after induction and 30 minutes for 3 hours. The data obtained were analyzed using the One-way ANOVA (Analysis of Variance) test and the Tukey HSD test. Based on the results of the study, it was shown that the results of the phytochemical screening of durian leaves contained flavonoids, saponins, tannins, alkaloids, steroids/triterpenoids. Of the three doses that experienced a decrease in temperature that was not significantly different from paracetamol, namely at a control dose of 300 mg/kg, because this decrease in temperature was almost close to paracetamol which was positive.

Riau Islands is one of the provinces in Indonesia that has abundant biodiversity. Biodiversity is the basis of various treatments and industrial discoveries in the pharmaceutical field in the future, people especially in developing countries depen on plants as medical ingredients to maintain their health. Therefore, efforts are needed to determine the content of primary and secondary metabolites obtained through proximate analysis and phytochemical screening test in plants. This study aims to explore and document the content of plant compounds on the Sekilak beach, Batam City. Amphiroa fragilissima was positive contained flavonoids, steroids, and saponins, water content 36,31%, ash content 44,09%, protein content 3,21%, fat content 8% and carbohydrate content 8,39%. Halimeda opuntia positive contained flavonoids compunds, alkaloids, and water content 27,68%, ash content 33,70%, fat content 7%, protein content 0,25%, and carbohydrate contain 31,37%. Halimeda macroloba positive contains flavonoids compounds, steroids, saponins, and water content 53,17%, ash content 13,26%, fat content 6%, protein content 1,00%, and carbohydrate content 26,57%.

Secang wood from the Caesalpiniacea tribe which was commonly found in Indonesia had secondary metabolites as antibacterial. This study aims to formulate secang wood ethanol extract into a transparent solid soap that is able to meet quality requirements. This research method was begin with formulating the extract into a transparent solid soap preparation with 3 concentrations, namely 5g (F1), 10g (F2), and 15g (F3). Furthermore, appearance tests, foaming ability, free alkali, free fatty acids, irritation, hedonic, and pH were carried out. The results of the research on transparent solid soap gave different colors for each concentration, namely transparent bright red (F1) and transparent dark red (F2 and F3) which were stable for 4 weeks; the foam power test shows a height of 4.5-6.5 cm after 10 minutes; free alkaline test shows a value of 0.07-0.08%; free fatty acid test 0.16%, and pH test 8.96-9.66 at week 4; and transparent solid soap does not cause irritation, attractive and liked by volunteers

Secang wood is one of the plants that contains many antioxidants, secondary metabolites and antibacterial compounds. This study aims to determine characterization of simplicia and chemical substances of Secang wood extract. Secang wood which was already in the form of simplicia was extracted by maceration using 96% ethanol solvent and the extract was concentrated with a rotary evaporator at a temperature of 40°C, then screening and characterization tests were carried out. The results of this study showed that phytochemical screening of the ethanol extract of sappan wood (Caesaloinia sappan L) showed the presence of flavonoid, tannin, saponin, alkaloid, steroid, and phenolic compounds. Characterization of simplicia obtained results at water content (3.95%) water soluble extract (11.01%), ethanol soluble extract (17.22%), total ash content (1.81%) and non-soluble ash content. acid soluble (0.28%).

Turi (Sesbania grandiflora L) is native to Southeast Asia. This plant is used as a medicinal plant and also a vegetable. Turi (Sesbania grandiflora L) is a plant that is efficacious for the treatment of various diseases such as analgesic, antipyretic, diuretic and laxative. The purpose of this study was to identify the content of secondary metabolites in turi leaf extract extracted with ethanol, ethyl acetate and n-hexane as solvents. This research was conducted in two stages, namely extraction by maceration method using ethanol, ethyl acetate and n-hexane as solvents. And the identification stage of secondary metabolites consisting of identification of alkaloids, steroids, terpenoids, saponins and flavonoids. The results showed that the positive ethanol and ethyl acetate extracts contained flavonoids, saponins, terpenoids and steroids, while the negative n-hexane extract contained secondary metabolites.

Parijoto fruit (Medinilla speciosa Blume) is an epiphytic shrub with a height of 0.45-1.2 meters. Parijoto fruit contains secondary metabolites of flavonoids, anthosionins, glycosides and others which have pharmacological effects as antioxidants, antidiabetic, anticancer, antibacterial, anti-inflammatory. Parijoto plant growth is influenced by temperature, humidity, rainfall and altitude where it grows. This study aims to determine the total flavonoid content in Parijoto fruit from Kudus and Semarang districts. This type of research is an experimental laboratory research using UV-Vis spectrophotometry method using quercetin and rutin as a comparison. The total flavonoids of Parijoto fruit extract from Kudus Regency and Semarang, respectively, with a quarcetin ratio of 81.60 mgQE/g and 76.48 mgQE/g, while with a routine comparison of 79.33 mgRE/g and 73, respectively. 06 mgRE/g. The total flavonoids of the two extracts showed quite strong results, but were significantly different as evidenced by the value of the paired sample t-test between the two extracts with a significance value (2-tailed) <0.05.

Bintaro (Cerbera manghas L.) is widely used as an analgesic, anticonvulsant, cardiotonic, and hypotensive activity. In Thailand bintaro is used as traditional medicine such as bark used as an antipyretic, laxative and in the treatment of dysuria. The flower part is applied to treat hemorrhoids. Secondary metabolites are chemical compounds contained in plants that can be drawn by a suitable solvent through extraction methods. The purpose of this study was to determine the content of secondary metabolites contained in bintaro leaf extract using ethanol, acetone, and n-Hexan solvents based on phytochemical screening. This research was carried out through two stages, namely extraction using maceration method using ethanol, acetone, n-Hexan and phytochemical screening solvents consisting of screening alkaloids, terpenoids, flavonoids, steroids, saponins, and tannins. The results showed that the bintaro leaf ethanol extract positively contained flavonoids, saponins, and tannins. The acetone extract of bintaro leaves positively contains, saponins and tannins. N-hexan daun bintaro extract positively contained steroids and alkaloids in testing with Dragendorft reagents, and was positive for Wagner reagents and negative for testing with Mayer reagents