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Akhmad Subarkah; Edy Susanto; Agung Nugroho Setiawan

Journal of Health Sciences, Public Health and Pharmacy 2025 International Forum of Researchers and Lecturers

Chronic kidney disease (CKD) is a progressive and irreversible decline in kidney function that, if left untreated, can lead to serious complications. Ultrasonography (USG) is a widely used imaging modality for detecting CKD, yet its interpretation remains highly dependent on the radiologist’s expertise. This study aims to develop a CKD detection system using a convolutional neural network (CNN) on kidney ultrasound images based on estimated glomerular filtration rate (eGFR), and to evaluate the system’s performance. This research employed a research and development (R&D) approach with an experimental design. The dataset consisted of kidney ultrasound images from CKD and non-CKD patients with corresponding eGFR values. The methodology included image preprocessing, CNN model training, and accuracy evaluation using classification metrics. The results demonstrated that the developed CNN model achieved a total accuracy of 97% on internal test data and 95.8% on external validation. The model’s sensitivity reached 100% for the normal category, 91.67% for CKD stage 4, and 90% for CKD stage 5. Specificity exceeded 96% across all categories, with high precision and F1-scores above 94% for all classes. This system has proven to be effective as a diagnostic support tool for automatically detecting CKD through kidney ultrasound imaging. Its advantages lie not only in accurately classifying CKD from USG images but also in correlating the classification results with patients' eGFR values. This provides more precise clinical information and supports appropriate CKD staging and management planning.

Amelia Pujalestari, Dea

Journal of Health Sciences, Public Health and Pharmacy 2025 International Forum of Researchers and Lecturers

This research aims to describe the implementation of Physical Education learning at Madrasah Pondok Pesantren Miftahul Muta'alimin (Miftahul Muta'alimin Islamic Boarding School). Learning implementation in this research includes planning, implementation, and assessment/evaluation, as well as the obstacles and supports that influence these implementations. This research used a descriptive qualitative approach. The data were obtained through interviews, observations, and documentation. The data validity was tested by using source triangulation. Observations and documentation were used as supporting data to strengthen the interview results. The research subjects consisted of the principals of Islamic Junior High Schools (MTs) and Islamic Junior High Schools (MA), a Physical Education teacher, and four students (two from each MTs and two from the MA). The data analysis was conducted through data reduction, data presentation, and conclusion drawing. The research findings indicate that the implementation of Physical Education learning has followed the planning, implementation, and evaluation stages, but has not been optimal. Planning remains administrative in nature and has not fully adapted to student characteristics. Learning implementation includes an introduction, core, and closing phase, but lacks of method variation and active student involvement. Assessment/evaluation has covered cognitive, affective, and psychomotor aspects, but it is still limited to final assessments and it is not yet sustainable. Furthermore, limited infrastructure and teacher backgrounds pose challenges. Despite this, madrasah and Islamic boarding schools (pesantren) generally support the implementation of Physical Education (PE) learning.

Nuraen Umar; Anik Purwati

Journal of Health Sciences, Public Health and Pharmacy 2025 International Forum of Researchers and Lecturers

Introduction: Children of all ages can get tuberculosis (TB), an infectious disease. With 500,000 child TB cases annually and 40–50% of the population in poor nations being categorized as children, child TB is a significant disease to research. This study's main goal was to ascertain how nutritional status, contact history, and BCG immunization status related to childhood tuberculosis at Tidore Hospital in 2025.  Methods: This kind of study used a cross-sectional design and was analytically descriptive. In December 2025, there were 80 children with tuberculosis who visited the polyclinic on a regular basis, making up the study's population. Contact history has a significance level of 0.000 <0.05 and a t value of 3.564 > t table 1.665. This indicates that the Child TB variable is significantly impacted by the contact history variable to some extent. The t count value for immunization status is 4.055 > t table 1.665, with a significance of 0.000 <0.05. This indicates that the Child TB variable is significantly impacted by the Immunization Status variable to some extent. The Nutritional Status hypothesis was tested, and the results reveal a t value of 3.044 > t table of 1.665 with a significance of 0.000 <0.05. This indicates that the Child TB variable is significantly impacted by the Nutritional Status variable to some extent.So the advice for health workers is that it is hoped that they will be more active in providing counseling or KIE, putting up poster media, and providing leaflets related to TB in children.

Nishpu Ramadhan R; Irmawat Irmawat; Musfirah Musfirah

Journal of Health Sciences, Public Health and Pharmacy 2025 International Forum of Researchers and Lecturers

A high-sodium and low-potassium consumption pattern can increase the risk of hypertension. Excess sodium can cause fluid retention and increase blood volume, while potassium plays a role in regulating fluid balance and blood pressure. This study aims to determine the relationship between high-sodium and high-potassium consumption patterns and the incidence of hypertension at the City Community Health Center (UPT Puskesmas) in Bantaeng Regency. The research method used was quantitative analytic with a cross-sectional study approach. The study population was all 278 patients visiting the City Community Health Center (UPT Puskesmas) in Bantaeng Regency. The sample size was 74 residents of the community at the City Community Health Center (UPT Puskesmas). Purposive sampling was used. The research instrument used was a questionnaire, which was distributed to respondents. The Food Frequency Questionnaire (FFQ) was used for sodium and potassium consumption. The results showed a relationship between sodium consumption and the incidence of hypertension (p=0.003 meaning Ha was accepted.The results showed a relationship between potassium consumption and the incidence of hypertension (p=0.001) meaning Ha was accepted. Researchers hope that this research can be used as a support or source of information to develop knowledge in the field of community health, especially in nursing, related to sodium and potassium consumption in reducing blood pressure in hypertension patients.

Lius Pongo; Edy Susanto; Yeti Kartikasri

Journal of Health Sciences, Public Health and Pharmacy 2025 International Forum of Researchers and Lecturers

Background: There are still shortcomings in the implementation of a truly safe and optimal thoracic examination protocol for toddlers in certain hospital settings. Furthermore, data related to direct radiation dose measurements and evaluation of the effectiveness of thoracic examination techniques for toddlers specifically in the local context in Indonesia are very limited. Objective: To examine the thoracic examination procedure that can be performed with a high level of safety without compromising the quality of diagnostic results and to evaluate the radiation exposure dose and thoracic examination techniques in toddlers at Hospital. Methodology: This study used a mixed methods approach with a convergent parallel design. Quantitative data were obtained from radiation dose measurements and examination parameters, while qualitative data were collected through observation, interviews, and group discussions, then analyzed thematically to understand the factors that influence radiation dose in infant thoracic examinations. Results: Research on thoracic radiology examinations in toddlers at Heart and Vascular Hospital was conducted systematically and in accordance with established procedures. Some limitations emerged from limited radiation dose records and inconsistent use of protective shields. Efforts to reduce radiation exposure include optimizing examinations, proper collimation, selecting exposure parameters, and educating families and staff. Continuous training and strict implementation of standard operating procedures (SOP) are essential to raise awareness of the ALARA principle. Internal policies and routine oversight are also needed to improve radiation dose monitoring, with the hope of improving the quality of radiology services and optimizing protection for toddler patients.

Aida Fitria; Devi Nallappan; Nuraini Nuraini; Khairatunnisa Khairatunnisa; Sumardin Moho

Journal of Health Sciences, Public Health and Pharmacy 2025 International Forum of Researchers and Lecturers

Based on data from the South Nias District Health Office, the number of hypertension cases at Bawomataluo Public Health Center increased steadily from 2022 to 2024. This condition posed a high risk of leading to severe complications such as heart disease, stroke, kidney failure, and damage to other organs. This study aimed to examine the factors influencing compliance with blood pressure control among hypertensive patients within the working area of Bawomataluo Public Health Center in the year 2025. This research employed a quantitative method using an analytic survey design with a cross-sectional approach. The population consisted of 185 individuals who had been diagnosed with hypertension over the past six months (from July to December 2024). A purposive sampling technique was used to select 127 participants. Data analysis included univariate, bivariate, and multivariate analyses. Using logistic regression with the forward method, the results showed that knowledge had a significance value of 0.002 with an Exp(B) of 5.680; family support had a significance value of 0.000 with an Exp(B) of 6.740; motivation had a significance value of 0.000 with an Exp(B) of 6.947; and self-confidence had a significance value of 0.001 with an Exp(B) of 5.706. Among these, motivation was identified as the most dominant factor due to having the highest odds ratio (Exp(B)). The findings indicated that knowledge, family support, motivation, and self-confidence significantly affected compliance with blood pressure control among hypertensive patients at Bawomataluo Public Health Center. Motivation emerged as the most influential factor. It was recommended that the health center actively provide support and encouragement to patients suffering from hypertension.

Christina Puspa Dewi; Yeni Rusyani; Dwi Puji Susanti

Journal of Health Sciences, Nursing and Nutrition 2025 International Forum of Researchers and Lecturers

Diabetes Mellitus (DM) is a chronic metabolic disorder that is rapidly increasing globally and is associated with a high risk of peripheral arterial complications. The Ankle Brachial Index (ABI) is a simple and reliable tool for screening peripheral arterial disease, while foot exercise represents a practical, low-cost, and non-pharmacological intervention to improve peripheral perfusion. This study aimed to evaluate the effectiveness of foot exercise in improving ABI values among hospitalized patients with DM. A pre-experimental one-group pretest-posttest design was implemented with 26 purposively selected inpatients with DM. Participants performed standardized foot exercises once daily for 15 minutes over two consecutive days. ABI values were measured using a sphygmomanometer and vascular Doppler before and after the intervention, and data were analyzed using a paired t-test. The mean ABI significantly increased from 0.707 (SD = 0.031) to 0.884 (SD = 0.027) (p < 0.001), indicating a clinically meaningful improvement from moderate impairment to near-normal circulation. These results demonstrate that foot exercise is effective in improving ABI values among hospitalized DM patients. The study highlights foot exercise as a safe, simple, and non-pharmacological nursing intervention that can be recommended in inpatient care settings to improve peripheral perfusion and prevent further complications related to peripheral arterial disease. This intervention offers a promising approach to managing peripheral arterial complications in diabetic patients

Ida Erna Widiyawati; Sri Setiatjahjati

Journal of Health Sciences, Public Health and Pharmacy 2025 International Forum of Researchers and Lecturers

Type 2 Diabetes Mellitus (T2DM) is a chronic condition that requires optimal adherence to pharmacological treatment to prevent disease progression and complications. This study aims to analyze the level of adherence to anti-diabetic medication among T2DM patients and to explore the association between the experience of medication side effects and adherence levels. A descriptive-analytic cross-sectional design was employed, involving 97 T2DM patients in Bandung Regency and Bandung City, Indonesia. Data were collected through structured questionnaires and analyzed using descriptive statistics and the Chi-Square test. The results revealed that 53.6% of respondents exhibited low medication adherence. The majority reported having diabetes-related complications (88.7%) and experiencing medication side effects (57.7%). A significant positive association was found between medication side effects and adherence level (p = 0.039), indicating that patients who experienced side effects tended to be more adherent. Demographic factors such as place of residence, gender, and age showed no significant association with adherence. The findings suggest that side effects may hinder adherence; however, awareness of the body’s response to medication, including side effects, may also serve as a motivator for adherence.

Sudana Fatahillah Pasaribu; Tuty Hertati Purba; Tiara Aribah Sahara; Penti Mawaddah; Annisa Zahra +3 more

Journal of Health Sciences, Public Health and Pharmacy 2025 International Forum of Researchers and Lecturers

Nutritional status is an overview of the condition of the body that reflects the balance between nutrient intake and the body's nutritional needs. Breakfast habits and the amount of pocket money are factors that can affect the nutritional status of school-age children. Objective: This study aims to determine the relationship between breakfast habits and the amount of pocket money with the nutritional status of students at Karya Bakti Helvetia Private Elementary School. Methods: This research employed an analytic survey method with a cross-sectional design. Results: The results showed that there was a significant relationship between breakfast habits and students’ nutritional status (p-value = 0.002; p < 0.05) and a significant relationship between the amount of pocket money and students’ nutritional status (p-value = 0.002; p < 0.05). Conclusion: It can be concluded that breakfast habits and the amount of pocket money are significantly associated with the nutritional status of students at Karya Bakti Helvetia Private Elementary School.

Sartika Maulida Putri; Dedy Firdaus Ridwan; Iriani Iriani; Nurpida Nurpida; Yenni Fitri Velayati

Journal of Health Sciences, Public Health and Pharmacy 2025 International Forum of Researchers and Lecturers

Completeness, suitability, and accuracy in filling medical record documents are essential for effective management of patient files, as well as for improving the overall quality of healthcare services. Medical records serve as a critical tool for documenting patient histories, treatments, and decisions made during their healthcare journey, thus impacting the quality of care provided. Incomplete or inaccurate documentation can lead to delays in treatment, miscommunication among healthcare providers, and legal issues related to patient care. Studies have shown that comprehensive medical records are linked to improved patient outcomes and enhanced operational efficiency in healthcare settings (Smith et al., 2019; Johnson & Patel, 2021). This study aimed to assess the completeness of patient medical record documents in the Medical Resume and Informed Consent forms for inpatients with surgical cases at Tgk. Chik Ditiro Sigli General Hospital. Using a descriptive quantitative approach, the research analyzed a random sample of 293 medical documents. The findings revealed that the completeness of medical record documentation was 69% for the Medical Resume sheet and 83% for the Informed Consent sheet. The study concluded that certain sections were either incomplete or omitted, primarily due to the assumption that certain fields were not applicable to the particular patient’s case. This highlights the need for further training and awareness for healthcare staff regarding the importance of complete medical documentation. The hospital should implement stricter guidelines for filling out medical records and ensure that healthcare workers fully understand the significance of accurate and complete documentation. Future research could explore strategies for improving the consistency of medical record documentation and evaluate the impact of training programs on filling medical records (Williams et al., 2020).

Harnanik Nawangsari; Raudhatul Munawarah; Rosita Syaripah

Journal of Health Sciences, Nursing and Nutrition 2025 International Forum of Researchers and Lecturers

Preeclampsia is a severe pregnancy complication characterized by hypertension and signs of organ damage, with a multifactorial etiology involving maternal, genetic, and environmental interactions. This study aims to analyze determinants contributing to the occurrence of preeclampsia through a comprehensive literature review. The research method involved an in-depth analysis of 15 scientific journals, consisting of 8 national publications from Google Scholar and 7 international publications from PubMed, selected based on relevance, credibility, and publication date. Data from these sources were synthesized to identify common risk factors, biological mechanisms, and preventive measures. The findings indicate that the primary risk factors for preeclampsia include a history of chronic hypertension, obesity, gestational diabetes, nulliparity (first pregnancy), and a family history of preeclampsia. Nutritional deficiencies, particularly low calcium and vitamin D intake, are also significantly associated with increased risk. Furthermore, immunological factors such as abnormal maternal immune tolerance, as well as placental dysfunction leading to poor perfusion, play a crucial role in the pathogenesis of preeclampsia. The interplay between these factors suggests that preeclampsia is not caused by a single mechanism, but rather by a complex network of physiological, metabolic, and genetic influences. This complexity underscores the importance of early risk factor identification and intervention strategies during antenatal care. Preventive efforts may include nutritional supplementation, weight management, blood pressure monitoring, and targeted medical interventions for high-risk individuals. In conclusion, preeclampsia remains a significant contributor to maternal and perinatal morbidity and mortality worldwide. Understanding its determinants enables healthcare providers to implement timely preventive measures and reduce its negative impacts on both mother and fetus. Further research is warranted to elucidate the precise mechanisms underlying these determinants, potentially paving the way for more effective prevention and treatment strategies.

Sudana Fatahillah Pasaribu; Farhatun Nurha; Dhea Syafitri; Dwitri Amalia Susanti; Etika Lase +3 more

Journal of Health Sciences, Nursing and Nutrition 2025 International Forum of Researchers and Lecturers

The nutritional status of elementary school children serves as a vital indicator of their overall growth, development, and future health outcomes. Poor nutritional status, whether in the form of undernutrition or overweight, can lead to a range of health and academic performance issues. This study aimed to assess the nutritional status of Grade 4A students at SD 064981 Cempaka Raya using anthropometric measurements and to examine the association between snacking habits and nutritional categories. A descriptive quantitative research design was employed, utilizing a total sampling approach involving all 20 students in the class. Data collection consisted of direct height and weight measurements conducted according to standardized procedures. The data were then analyzed using the WHO Anthro Plus software to classify nutritional status into undernourished, normal, overweight, and obese categories. Additionally, information on students’ snacking habits during school hours was obtained through structured questionnaires. The results indicated that 25% of students were undernourished, 50% had normal nutritional status, 20% were overweight, and 5% were obese. Bivariate analysis using the Chi-Square test demonstrated a statistically significant relationship (p < 0.001) between snacking behavior and nutritional status. Specifically, all students who reported frequent consumption of snacks at school were classified as overweight or obese, whereas those who did not engage in frequent snacking predominantly fell within the undernourished or normal categories. These findings highlight the potential role of unhealthy snacking patterns in contributing to excessive weight gain among school-age children. Based on the results, it is recommended that nutrition education programs be implemented for both students and parents, coupled with stricter regulations on the availability and sale of high-calorie snack foods within the school environment. Such measures may serve as preventive strategies to promote healthy growth, maintain optimal nutritional status, and reduce the risk of non-communicable diseases later in life.

Elisa Silvia Aritonang; Ronni Naudur Siregar; Nurcahaya Nainggolan; Dewi Carolina Panjaitan

Journal of Health Sciences, Public Health and Pharmacy 2025 International Forum of Researchers and Lecturers

Nausea and vomiting are common discomforts experienced by 50% of pregnant women in the first trimester. One of the implementations to reduce nausea and vomiting with non-pharmacological therapy is the provision of lavender aromatherapy. The purpose of this study was to determine whether there was an effect of giving Lavender aromatherapy on nausea and vomiting in pregnant women in the first trimester at PMB Ronni Siregar Deli Serdang. The research method used was Quasi-experimental using nonequivalent control group design. The sampling technique used in this study was Total sampling based on Inclusion and Exclusion criteria, the number of samples was 30 respondents (15 intervention groups and 15 control groups). The results showed that the average value of respondents in the intervention group in the nausea and vomiting value before giving lavender aromatherapy was Mean 13.5 standard deviation 3.521. in the intervention group after being given treatment, a mean value of 10.6 was obtained with a standard division of 4.459. Based on the t-test, a p-value of 0.000 was obtained, which means it is smaller than the significant value <0.05. The conclusion of this study is that there is an Effect of Lavender Aromatherapy on Nausea and Vomiting in Pregnant Women in the First Trimester at PMB Ronni Siregar Deli Serdang. This study expects Midwives to provide health promotion and education, namely by improving their midwifery care, especially for Pregnant Women with nausea and vomiting during pregnancy, in addition to explaining the non-pharmacological benefits in reducing nausea and vomiting during pregnancy.

Didit Damayanti

Journal of Health Sciences, Nursing and Nutrition 2025 International Forum of Researchers and Lecturers

Indonesia, based on its geographical location, is a country that is difficult to escape from the threat of natural disasters, one of which is earthquakes. The high number of casualties from natural disasters indicates that the community's emergency response abilities are still very poor, especially among children who lack basic knowledge in disaster management. The purpose of this research is to determine the impact of education and singing to  earthquake emergency response abilities among students at MI Rejoagung. This study used a pre-experimental research design with a one-group pretest-posttest approach. The sampling technique resulted in 30 respondents. Data collection was done through interviews and observations, and data analysis used the Paired T-Test. The research findings showed that before the intervention, the majority (57%) of respondents had sufficient abilities with a mean of 10.96. After the intervention, almost all (83%) of the respondents had good abilities with a mean of 24.78. The analysis result yielded a p-value of 0.000 (≤ α 0.05), indicating that there is an influence of education and singing to  disaster earthquake response abilities. The education and singing to makes it easier to understand the material and procedures, makes learning more interesting, and is easy to remember. Respondents are advised to continue improving their knowledge and abilities in disaster preparedness through various sources of information and active participation in disaster training or education.

Rahmadina Khasanah; Wahyu Wijaya Widiyanto

Journal of Health Sciences, Public Health and Pharmacy 2025 International Forum of Researchers and Lecturers

Electronic Medical Record (EMR) systems have become a fundamental component of hospital information systems, aiming to improve the quality, efficiency, and continuity of patient care. However, the success of EMR implementation depends largely on user satisfaction. This study investigates user satisfaction with the EMR system implemented at Dr. Soetarto (DKT) Hospital, a mid-tier hospital in Yogyakarta, Indonesia. The research addresses issues related to usability, data accuracy, and system responsiveness that potentially hinder effective EMR usage. The objective is to evaluate user satisfaction using the End User Computing Satisfaction (EUCS) framework, which consists of five dimensions: content, accuracy, format, ease of use, and timeliness. A quantitative approach was applied through a structured questionnaire distributed to 40 healthcare workers, including medical records staff, nurses, pharmacists, and administrative personnel. Data analysis using Spearman correlation revealed significant positive relationships among all EUCS dimensions, with the strongest association found between format and ease of use. The findings indicate that while users are generally satisfied with the system’s performance, improvements are still needed in timely data retrieval and user interface consistency. This study highlights the critical need for continuous system evaluation and end-user involvement to optimize EMR functionality and healthcare service delivery.

Vivi Rahmawati; Wahyu Wijaya Widiyanto

Journal of Health Sciences, Nursing and Nutrition 2025 International Forum of Researchers and Lecturers

Electronic Medical Records (EMR) are essential for improving the quality, efficiency, and continuity of healthcare services. Despite mandates requiring full implementation of EMRs in Indonesian healthcare facilities by the end of 2023, many hospitals continue to face challenges in achieving effective adoption. This study aims to analyze the implementation of EMRs in the outpatient services of Nur Hidayah Hospital, Yogyakarta, using the 5M framework: Man, Money, Machine, Materials, and Method. Employing a qualitative descriptive approach, data were collected through in-depth interviews, observations, and document analysis involving key informants from medical records staff, registration officers, and IT personnel. The findings revealed that while the availability of staff and supporting infrastructure such as computers and software was generally adequate, limitations persisted in human resource specialization, training access, and system integration. Financial planning for EMR-related needs was done through annual budgeting (RAB), but coordination between departments remained minimal. Key challenges included slow internet connectivity, limited IT staff, and lack of specific Standard Operating Procedures (SOPs) for EMR usage. Overall, the EMR system has been implemented in most outpatient units, yet not all units have fully transitioned due to technical and organizational barriers. This study concludes that optimizing EMR implementation requires not only technological readiness but also organizational alignment, continuous staff development, and robust inter-departmental coordination.

Ni Luh Putu Eka Kartika Sari; Putu Nia Calista Santoso; Ni Putu Diah Witari; I Gusti Ngurah Agung Adi Primantara; I Putu Bhujangga Pratama Kusuma Artana +2 more

Journal of Health Sciences, Public Health and Pharmacy 2025 International Forum of Researchers and Lecturers

Indonesia's biodiversity holds great potential for drug development, including through the use of traditional medicinal plants such as tapak liman (Elephantopus scaber L.), suruhan (Peperomia pellucida L. Kunth), and temu ireng (Curcuma aeruginosa Roxb.). These plants have been used in traditional medicine and are known to contain bioactive compounds with potential anticancer properties. Tapak liman contains compounds capable of inducing apoptosis in cancer cells. Suruhan is known to have cytotoxic and immunomodulatory effects, while temu ireng contains compounds with antioxidant and anticancer activity. This study aims to identify and quantify the bioactive compounds in these three plants using Gas Chromatography-Mass Spectrometry (GC-MS) and to explore their therapeutic potential in cancer treatment. The results of GC-MS analysis successfully identified six main compounds that have potential as anticancer agents, namely Phytol, Caryophyllene, Apiol, Germacrone, Germacrene B, and one additional compound. Temu ireng contains Germacrone, Germacrene B, and Caryophyllene, while suruhan contains Caryophyllene, Apiol, and Phytol. Meanwhile, tapak liman shows the presence of bioactive compounds that support cytotoxic activity. The focus of this study is on the interaction of bioactive compounds with the Bcl-2 protein, which plays a role in inhibiting apoptosis in cancer cells. Additionally, molecular analysis revealed that Germacrone, Germacrene B, Caryophyllene, and Apiol can also bind to the p53 protein, known as a guardian of the genome and a trigger for apoptosis. These findings suggest that the three plants have high potential as natural sources of anticancer agents. Further in vitro and in vivo studies are recommended to test the biological efficacy of these compounds in the context of cancer therapy, thereby bridging traditional knowledge with modern scientific approaches in the development of natural-based medications.

Retno Wilujeng; Eva Harlina; Rini Madyastuti Purwono; Dimas Andrianto

Journal of Health Sciences, Public Health and Pharmacy 2025 International Forum of Researchers and Lecturers

Nephrolithiasis, commonly known as kidney stone disease, can be experimentally induced in animal models using ethylene glycol (EG) in combination with ammonium chloride, which closely mimics calcium oxalate stone formation in humans. This condition is associated with significant renal tissue injury, including glomerular atrophy, tubular necrosis, and hyaline droplet accumulation, ultimately impairing kidney function. The present study aimed to investigate the histopathological changes in the kidneys of EG-induced rats and evaluate the nephroprotective potential of combined avocado (Persea americana) and gooseberry (Physalis angulata) leaf extracts. A total of 30 male Sprague Dawley rats were randomly divided into five groups: a standard control (no induction), a negative control (EG-induced without treatment), a positive control (EG-induced and treated with a commercial nephroprotective agent), and two treatment groups receiving the combined extracts at different doses. Nephrolithiasis induction was performed over 28 days, followed by oral administration of the respective treatments. At the end of the experiment, kidney tissues were collected and processed for histological examination using hematoxylin-eosin staining to assess the extent of tissue damage. The negative control group exhibited the most severe histopathological alterations, with hyaline droplet formation reaching 15.0 ± 5.25%. In contrast, extract-treated groups demonstrated a marked reduction in tissue damage, with the most significant improvement observed in the group receiving 300 mg/kg avocado extract and 100 mg/kg gooseberry extract, which recorded hyaline droplet formation of only 5.27 ± 2.74%, a result comparable to that of the standard control group. These findings suggest that the combination of P. americana and P. angulata leaf extracts confers protective effects against EG-induced renal injury. Therefore, this herbal combination may represent a promising natural nephroprotective agent that warrants further investigation in preclinical and clinical settings.

Silvy Aldila; Farah Destyana Nurainy; Ummi Kalsum; Akmalia Septi Haliza; Easter Kaniada Kristanti +2 more

Journal of Health Sciences, Public Health and Pharmacy 2025 International Forum of Researchers and Lecturers

The selection of appropriate solvents in sterile injection formulations plays a pivotal role in determining the stability, efficacy, and safety of pharmaceutical products. Among the commonly utilized solvents, Dimethyl Sulfoxide (DMSO), Polyethylene Glycol (PEG), and pyrogen-free water exhibit distinct physicochemical properties and functional roles. This study aims to analyze the characteristics, functions, and implications of these three solvents within the context of sterile injectable preparations. The research methodology involves a comprehensive review of six peer-reviewed scientific articles published within the last five years, focusing on solvent performance, compatibility, and safety profiles. Findings reveal that DMSO is highly effective in dissolving lipophilic compounds due to its strong solvating capacity. However, its use at elevated concentrations may lead to irritation and cytotoxicity, necessitating careful dosage regulation. PEG, particularly in its flexible molecular weight variants, serves as a co-solvent and viscosity modifier, enhancing solubility and stability of active pharmaceutical ingredients. Pyrogen-free water, characterized by its inertness and biocompatibility, remains the gold standard for injection solvents, offering minimal risk and broad applicability across drug classes. The study underscores the importance of aligning solvent selection with the physicochemical nature of the drug, intended route of administration, and patient safety considerations. Furthermore, the potential for combining solvents to achieve optimized formulations is highlighted as a promising avenue for future pharmaceutical development. Such combinations may offer enhanced solubility, reduced toxicity, and improved delivery mechanisms, particularly for complex or poorly soluble compounds. In conclusion, understanding the nuanced roles of DMSO, PEG, and pyrogen-free water is essential for formulating safe and effective sterile injections. This research contributes to the growing body of knowledge supporting rational solvent selection and encourages further exploration into innovative solvent systems for advanced drug delivery.

Eni Marwening Tyas; Nila Widya Keswara

Journal of Health Sciences, Public Health and Pharmacy 2025 International Forum of Researchers and Lecturers

The use of formula milk for infants is still common in the early stages of life and has the potential to hinder successful breastfeeding, even though the benefits of breast milk have been widely proven. Education and lactation counseling are considered important strategies to support breastfeeding practices and reduce the unnecessary use of formula milk. However, empirical evidence on the effectiveness of lactation counseling in reducing the intensity of formula milk use is still limited, especially in the context of primary health care in Indonesia. This study aims to analyze the effectiveness of lactation counseling in reducing the intensity of formula feeding in infants. This study uses a quantitative approach with a one-group pretest–posttest pre-experimental design. The sample consisted of 20 mothers who had infants and met the inclusion criteria in the Popayato Community Health Center working area, selected using total sampling technique. The intervention took the form of structured lactation counseling provided by health workers. The intensity of formula feeding was measured before and after the intervention using a structured questionnaire. Data analysis was performed descriptively and bivariately using the Wilcoxon Signed Rank Test. The results showed a decrease in the intensity of formula feeding after lactation counseling. The Wilcoxon test showed a significant difference between the intensity of formula feeding before and after education (p = 0.000). Lactation counseling was proven to be effective in reducing the intensity of formula feeding in infants. This educational intervention has the potential to be an important promotional strategy in maternal and child health services to support more optimal breastfeeding practices..