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Saleh Yaseen, Ahmed; Yosef Othman Homeda; Mohammad M. Al-Tufah; Mutlak Saud Khalaf; Mohannd Faisal Shareef

Jurnal Riset Ilmu Farmasi dan Kesehatan 2025 Asosiasi Riset Ilmu Kesehatan Indonesia

This study reports the green synthesis of vanadium pentoxide (V₂O₅) using virgin olive oil as a natural and environmentally benign reducing agent. The approach aims to minimize the environmental impacts associated with conventional synthesis routes. Structural and physicochemical characterizations confirmed the successful formation of nanoscale V₂O₅. X‑ray diffraction (XRD) analysis indicated an average crystallite size of approximately 16.57 nm, evidencing high crystallinity. Fourier‑transform infrared spectroscopy (FTIR) revealed characteristic V=O and V–O–V vibrations with bands associated with physisorbed water, confirming the correct oxide framework. Field‑emission scanning electron microscopy (FE‑SEM) showed irregularly shaped nanoparticles with a representative particle diameter of ~32.62 nm. Brunauer–Emmett–Teller (BET) and Barrett–Joyner–Halenda (BJH) analyses yielded a specific surface area of 10.817 m²/g, a total pore volume of 0.024277 cm³/g, and a broad mesoporous distribution (20–90 nm). Energy‑dispersive X‑ray spectroscopy (EDX) confirmed the purity of V₂O₅ with weight fractions of V (69.40%) and O (30.60%), consistent with the stoichiometric composition. Overall, the results demonstrate the effectiveness of olive oil as a green reducing agent for preparing nanoscale V₂O₅, which is promising for catalysis, energy storage, sensors, and clean‑energy applications.

Khairunnisa, Riska; Abdurahman, Abdurahman

Dinamika Pembelajaran : Jurnal Pendidikan dan bahasa 2025 Lembaga Pengembangan Kinerja Dosen

This research aims to analyze religious and structural values in Dede Soepriatna's short story "Titah yang Berkuasa" through an integrated religious and structural approach. Employing a qualitative descriptive method with content and structural analysis techniques, this study reveals various spiritual values implicit within the narrative of this contemporary Indonesian literary work. The analysis results demonstrate fundamental religious values such as destiny (qada and qadar), tawakkal (submission to God's will), sacrifice, betrayal, and divine justice. The character Roro Oyi represents spiritual steadfastness and surrender to the Creator amidst life's trials, while King Amangkurat's character critiques the abuse of power that contradicts moral-religious principles. Structurally, the short story presents a complex plot with effectively developed central conflict, multidimensional characterization, and rich symbolism that deepens the spiritual meaning of the narrative. The research findings confirm that literature can function as an effective medium for moral and religious education without sacrificing narrative integrity. The integration of religious and structural approaches provides a holistic perspective in understanding how spiritual messages are conveyed through formal beauty, offering a literary appreciation model that harmoniously combines ethical and aesthetic dimensions.

Putri Jihannisa; Wury Damayantie; Murtiyana Sari

Jurnal Ilmu Kesehatan 2025 Lembaga Pengembangan Kinerja Dosen

The wild ginger leaves (Costus speciosus) is used by the community as a traditional medicine, with its rhizome being utilized as an antipyretic, diuretic and anti-inflammatory agent. This plant contains secondary metabolite compounds such as alkaloids, flavonoids, polyphenols, quinones, tannins and saponins. This research aims to identify saponin compounds and characterize the moisture content and ash content in the ethanol extract of the white costus (Costus speciosus) leaves. The white costus leaves sample was extracted using the maceration method with 96% ethanol as the solvent. The extraction yielded a rendemen value of 13.3%. Non-specific characterization tests included moisture content and ash content. The results for the moisture content of the white costus leaf simplicia were 3.3%, which meets the quality requirement of not more than 10% according to Regulation of the Food and Drug Supervisory Agency Number 32 of 2019. The ash content test result for the simplicia was 0.97%, which also meets the standard requirement of the Indonesian Herbal Pharmacopoeia, which is less than 10.7%. Phytochemical screening was conducted to identify the types of compounds contained in the extract. Based on the phytochemical screening results, the 96% ethanol extract of white costus leaves positively contains alkaloids, tannins, saponins, and steroids.

Anggoro, Ari; Saputra, Fajar Anggi; Pratama , Dimas Ageng

Jurnal Rumpun Ilmu Bahasa dan Pendidikan 2025 Asosiasi Periset Bahasa Sastra Indonesia

This study aims to analyze and compare the poems Anant Prem by Rabindranath Tagore and Tentu, Kau Boleh by Sapardi Djoko Damono. The research employs a descriptive qualitative approach focusing on several literary aspects, namely the central theme, tone and atmosphere, diction, characterization, conceptual meaning of love, and philosophical perspective. The findings indicate that both poems share similarities in presenting love as a profound emotional experience, expressed through intimate and reflective nuances. Each poem portrays emotionally intertwined characters whose inner experiences revolve around the presence of love as a central force. Despite these similarities, the two poems differ significantly in how they interpret and narrate love. In Anant Prem, Tagore frames love as eternal and transcendent, surpassing the confines of time and space. This creates a contemplative and spiritual tone supported by metaphorical and symbolic diction. Meanwhile, Tentu, Kau Boleh portrays love as an immediate and personal experience rooted in everyday human interactions. Sapardi expresses love through calm, straightforward language that reflects simplicity and emotional closeness. Philosophically, Tagore positions love as part of cosmic existence, whereas Sapardi views it as a presence embodied in the warmth and intimacy of daily human life. These distinctions highlight how cultural background, worldview, and poetic tradition influence the depiction of love in both works.

Khaleel Ibrahim Ismael

International Journal of Health and Medicine 2025 Asosiasi Riset Ilmu Kesehatan Indonesia

Oxidative stress-induced apoptosis and chromatin instability are usually associated with male infertility and undermine the sperm DNA integrity. Recently, Vitamin K₂ (menaquinone) became a bioactive compound whose regulation action can be found in both apoptosis and transcriptional signaling. This study examines how Vitamin K₂ can be protective in the regulation of apoptotic pathways and stability in sperm chromatin by an integrative experimental and in silico analysis. Vitamin K₂ levels, DNA fragmentation level of infertile men's serum and semen samples (Aniline Blue), and the expression levels of apoptosis-associated genes (BAX, BCL-2, and CASP-3) in the samples were examined by qRT-PCR. The characterization of Vitamin K₂ binding affinity to apoptotic proteins was done using dynamics simulations. Findings showed that high levels of Vitamin K₂ had a significant positive correlation with reduced levels of DNA fragmentation and good control of apoptotic genes with reduced BAX and CASP-3 and increased expression of BCL-2. In general, the results indicate Vitamin K₂ as a potential regulator of sperm death and chromatin integrity that provides new therapeutic understanding on how male infertile patients can be treated.

Venty Lestari

Venus: Jurnal Publikasi Rumpun Ilmu Teknik 2025 Asosiasi Riset Ilmu Teknik Indonesia

The demand for lightweight materials with high mechanical strength has driven the development of aluminum alloys, particularly Al-Mg-Si, through deformation processes such as cold rolling. This study aims to analyze the effect of varying degrees of cold rolling deformation on the grain aspect ratio and macrohardness of homogenized Al-Mg-Si alloys. Deformation was applied at three thickness reduction levels—5%, 10%, and 20%—followed by microstructural characterization using optical microscopy and macrohardness testing in accordance with ASTM E-18 standards. The results show that increasing deformation levels lead to elongated grain morphology, with the grain aspect ratio rising from 1.16 to 2.07 and macrohardness increasing from 46.64 HRE to 62 HRE. The emergence of slip lines and grain flattening indicates the occurrence of intense plastic deformation, while work hardening results from dislocation accumulation that impedes further slip motion. These findings confirm a strong correlation between microstructural evolution and mechanical property enhancement in cold-deformed Al-Mg-Si alloys. This research contributes to the optimization of cold rolling parameters to produce engineering materials with a desirable balance of strength, formability, and fatigue resistance for applications in the mining and heavy manufacturing industries.

Heindrich Taunaumang; Ishak Pawarangan; Farly Tumimomor

Jurnal Pendidikan Kimia, Fisika dan Biologi 2025 Asosiasi Riset Ilmu Pendidikan Indonesia

Supercapacitor electrode material bassed on Pelepah Enceng Gondok Activated Carbon (KAPEG have been developed for increasing the performance of the supercapacitor application. The activated carbon surface structure and its properties strongly depend on the pyrolysis temperature and the type of activation methods. The bamboo carbon has been fabricated using pyrolysis method. The KAPEG was produced using chemical activation with H3PO4 as activation agent with variation of temperature activation 750oc have been carried out. Characterization of the surface structure (functional groups vibration) of bamboo carbon (CB) pyrolysis and the Pelepah enceng Gonddok activated carbon (KAPEG) were carried out using FTIR. The aim of this research is  to identify the functional groups vibration. he FTIR rssults shows absorption peeaks of functional groups of -OH, C=O, C-O, C-H and C-C vibration. The increasing of wavenumber of absorption peaks of functional groups -OH stretching, C=O stretching and C-H indicate that the functional groups bond for KAPEG samples becomes more stronger than for KPEG sample. The conclusion of this research are: the increasing of wavenumber of absorption peaks for functional groups indicates of more stronger of functional groups bond.

Filaili Ainun Jariyah; Indah Puspitasari; Raras Hafiidha Sari

Jurnal Ilmu Pendidikan, Bahasa, Sastra dan Budaya 2025 Asosiasi Periset Bahasa Sastra Indonesia

This study aims to find the values ​​contained in the novel shown through the characterization of the characters. These characterizations are related to the values ​​that occur in the lives of the characters in the novel Simfoni Persahabatan by Fakhri Violinist. This study uses a qualitative method with a structural approach that focuses on characterization and its relationship to educational values. Data were collected through document study techniques by carefully reading the novel and noting parts related to characterization, then analyzed using content analysis techniques to draw conclusions about the values ​​and messages contained in the novel. The results of the study show that the author successfully presents the characterization of two main characters, namely Nancy who has an arrogant nature and often insults others, and Bintang who is humble and always prays to God. Through the characterization of these two characters, the author conveys various educational values ​​including the value of simplicity, the spirit of pursuing ideals, moral education such as diligent study and humility, character education regarding respect for parents and teachers, the value of hard work, responsibility, patience in facing trials, the importance of admitting mistakes and forgiveness, and friendship. This research contributes to the world of education in utilizing children's literature as a medium for character formation, and provides references in selecting reading materials that suit the needs of children's character development.

Filaili Ainun Jariyah; Indah Puspitasari; Raras Hafiidha Sari

Jurnal Ilmu Pendidikan, Bahasa, Sastra dan Budaya 2025 Asosiasi Periset Bahasa Sastra Indonesia

This study aims to find the values ​​contained in the novel shown through the characterization of the characters. These characterizations are related to the values ​​that occur in the lives of the characters in the novel Simfoni Persahabatan by Fakhri Violinist. This study uses a qualitative method with a structural approach that focuses on characterization and its relationship to educational values. Data were collected through document study techniques by carefully reading the novel and noting parts related to characterization, then analyzed using content analysis techniques to draw conclusions about the values ​​and messages contained in the novel. The results of the study show that the author successfully presents the characterization of two main characters, namely Nancy who has an arrogant nature and often insults others, and Bintang who is humble and always prays to God. Through the characterization of these two characters, the author conveys various educational values ​​including the value of simplicity, the spirit of pursuing ideals, moral education such as diligent study and humility, character education regarding respect for parents and teachers, the value of hard work, responsibility, patience in facing trials, the importance of admitting mistakes and forgiveness, and friendship. This research contributes to the world of education in utilizing children's literature as a medium for character formation, and provides references in selecting reading materials that suit the needs of children's character development.

Lestari, Venty

Manufaktur: Publikasi Sub Rumpun Ilmu Keteknikan Industri 2025 Asosiasi Riset Ilmu Teknik Indonesia

Al–Mg–Si aluminum alloys are widely utilized in engineering applications due to their low density, excellent corrosion resistance, and mechanical properties that can be modified through heat treatment. This study investigates the effect of homogenization on the microstructure and hardness of Al–Mg–Si alloys produced by the squeeze casting process. The experimental procedure involved alloy melting, squeeze casting at 76 MPa using preheated metal molds, followed by homogenization at 400 °C for 4 hours. Microstructural characterization was performed using optical microscopy to examine the dendritic morphology and measure the secondary dendrite arm spacing (SDAS). Mechanical properties were evaluated through Vickers microhardness and Rockwell macrohardness testing. The results show that homogenization increases the SDAS from 32.59 μm to 36.88 μm and decreases the volume fraction of interdendritic phases from 15.51% to 13.57%. Furthermore, microhardness decreased from 50.22 VHN to 38.58 VHN, while macrohardness decreased from 54.60 HRE to 46.64 HRE. These reductions are attributed to the partial dissolution of Mg₂Si precipitates into the aluminum matrix during homogenization. Overall, this research provides valuable insight into the optimization of initial heat treatment parameters for Al–Mg–Si alloys produced by squeeze casting. The findings highlight the role of homogenization in improving microstructural uniformity and preparing the alloy for subsequent deformation processes such as cold rolling and extrusion, particularly for structural components used in mining and heavy transportation industries.

Mia Baizura; Nadila Khairunnisa; Salsabila Hasna Putri; Widya Rahayu Putri

Jurnal Pendidikan Kimia, Fisika dan Biologi 2025 Asosiasi Riset Ilmu Pendidikan Indonesia

This research focuses on the synthesis and characterization of the double salt copper(II) ammonium sulfate hexahydrate, Cu(NH4)2(SO4)2·6H2O. The study aims to obtain the compound in crystalline form and evaluate its properties through yield calculation, solubility testing, and Fourier Transform Infrared (FTIR) spectroscopy. The synthesis involved reacting copper(II) sulfate pentahydrate (CuSO4·5H2O) with ammonium sulfate ((NH4)2SO4) under controlled conditions, followed by crystallization. The process produced 10.84 grams of crystalline Cu(NH4)2(SO4)2·6H2O with an 86.23% yield, indicating efficient synthesis. Solubility tests showed that the crystals were polar, soluble in polar solvents like water and hydrochloric acid (HCl), partially soluble in ammonium hydroxide (NH4OH), and insoluble in less polar solvents like ethanol and chloroform (CHCl3). FTIR analysis confirmed the presence of functional groups such as O–H, N–H, and S–O stretching vibrations, supporting the proposed molecular structure. The findings demonstrate that Cu(NH4)2(SO4)2·6H2O can be efficiently synthesized, and its physicochemical properties align with theoretical expectations. This study contributes to the understanding of double salt synthesis and characterization, relevant for inorganic chemistry, material science, and potential applications in catalysis and coordination chemistry.

Lutfi, Mohammad; Azzahra, Fadlia; Pomuruh, Felix Kristianto; Manggau, Gemaliany Pilo; Syamsuddin, Syamsuddin +2 more

JITIPARI (Jurnal Ilmiah Teknologi dan Industri Pangan UNISRI) 2025 Universitas Slamet Riyadi Surakarta

Tofu is a common food made from soybeans. However, because soybeans have limitations in terms of availability, other alternative ingredients such as palm kernel meal (BIS) are needed. The purpose of this study was to determine the best concentration of palm kernel meal added to tofu making so as to reduce dependence on the use of soybeans. The study was designed using a completely randomized design with 1 factorial, namely the concentration of BIS and soybeans. The concentration variations used were: A1 (control, 100% soybean), A2 (25% BIS: 75% soybean), A3 (50% BIS: 50% soybean), and A4 (75% BIS: 25% soybean). The parameters tested were BIS crude fiber, proximate analysis and tofu organoleptic. The results showed that BIS after fermentation experienced a decrease in crude fiber from 27.25 to 18.375%. Based on the proximate analysis, the variation of 75% BIS: 25% soybean is the best concentration with the highest protein content of 45.14%. Likewise, the moisture, ash, fat and carbohydrate content obtained were 79.05; 1.00; 6.20; and 0.96%. The organoleptic showed the level of panelists' liking for color, aroma, texture and taste with the value of panelists' liking level respectively 3.11; 4.09; 3.6 and 3.96 which indicates that this product can be accepted by the community.

Ratna Widyaningsih; Edgie Yuda Kaesti; Dhika Permana Jati; Fahrur Rozi; Suwardi Suwardi +1 more

International Journal of Industrial Innovation and Mechanical Engineering 2025 Asosiasi Riset Ilmu Teknik Indonesia

Reservoir heterogeneity has long been recognized as a critical factor influencing the efficiency of enhanced oil recovery (EOR) methods. Among the techniques applied, cyclic waterflooding is considered one of the promising approaches due to its relatively simple operational design and potential to improve sweep efficiency. This method involves alternating water injection in specific cycles to mobilize trapped oil and redistribute reservoir pressure. However, the variation in geological properties such as porosity, permeability, and fluid saturation creates challenges in achieving uniform displacement, especially in reservoirs with high heterogeneity. Understanding the role of heterogeneity is therefore crucial for optimizing cyclic waterflooding applications. This study applies a literature review approach by synthesizing findings from previous experimental and field studies that evaluated cyclic waterflooding under different reservoir conditions. The analysis compares the performance of cyclic water injection periods across reservoirs characterized by varying levels of heterogeneity. Parameters such as injection rate, water breakthrough time, and oil recovery factor were considered in evaluating the effectiveness of this method. The results highlight that reservoirs with high heterogeneity often experience uneven fluid distribution, leading to early water breakthrough and reduced oil recovery. In contrast, reservoirs with relatively low heterogeneity tend to respond better to cyclic waterflooding, resulting in improved sweep efficiency and higher incremental recovery. Moreover, the optimization of cycle timing and water injection intervals appears to significantly mitigate the negative effects of heterogeneity. In conclusion, the study emphasizes that reservoir heterogeneity plays a decisive role in determining the success of cyclic waterflooding. Tailoring injection strategies based on geological variability is essential to maximize recovery efficiency. Future research should focus on integrating advanced reservoir characterization techniques with adaptive cyclic flooding models to further enhance oil production outcomes.

Evelyn Luna Anggraini; Ardi Mustakim

Jurnal Pendidikan Kimia, Fisika dan Biologi 2025 Asosiasi Riset Ilmu Pendidikan Indonesia

Solid waste from the tapioca processing industry, which is based on cassava (Manihot esculenta), is a medium rich in organic matter, particularly starch, that supports the growth of various types of microorganisms, especially fungi and bacteria. This study aims to isolate and observe the morphology of microorganisms from solid waste of cassava from the tapioca industry using Potato Dextrose Agar (PDA) medium in a series of pharmaceutical microbiology practicum. Waste samples were taken from the tapioca processing site, then underwent a serial dilution process and inoculation into PDA medium, which was incubated at 28–30°C for 5 days. The isolation results showed the growth of microorganisms with diverse colony morphologies, which are suspected to originate from the genera Aspergillus, Penicillium, and Rhizopus, based on macroscopic and microscopic characteristics. The presence of these microorganisms indicates the potential utilization of cassava waste as a source of microbial isolates for biotechnological and pharmaceutical applications, such as enzyme production and development of bioactive compounds. This practicum activity also contributes to increasing students' understanding of isolation techniques, microorganism characterization, and their role in biological-based waste management.

Tata Anggraini; Ardi Mustakim

Pentagon : Jurnal Matematika dan Ilmu Pengetahuan Alam 2025 Asosiasi Riset Ilmu Matematika dan Sains Indonesia

Tempoyak is a traditional fermented product derived from the flesh of durian fruit (Durio zibethinus) that has undergone a spontaneous fermentation process. This product is very popular in various regions in Indonesia, especially in Sumatra and Kalimantan, and is usually consumed as a cooking spice or side dish. The fermentation process primarily involves the activity of lactic acid bacteria (LAB), a group of microorganisms capable of converting acidic sugars into lactate. LAB plays an important role in forming the distinctive taste of tempoyak, lowering pH, inhibiting the growth of pathogenic microbes, and potentially providing health benefits for consumers. This study aims to isolate and characterize LAB from tempoyak using a microbiological approach. Tempoyak samples were taken from traditional markets, then isolated using MRS agar measurement media. Characterization was carried out through observation of colony morphology, Gram staining, and biochemical tests such as catalase and fermentation of various types of sugars (glucose, sucrose, and maltose). The isolation results showed that all isolates had the typical LAB characteristics, namely Gram-positive, catalase-negative, and rod- and coccus-shaped. The different sugar fermentation abilities indicate species diversity, with the suspected dominant genera being Lactobacillus, Streptococcus, Enterococcus, and Lactococcus. Furthermore, several isolates demonstrated antibacterial activity against Escherichia coli, indicating the potential use of LAB from tempoyak as a probiotic or natural preservative in the food industry. The decrease in pH from 6.2 to 3.6 during the fermentation process also confirmed the metabolic activity of LAB in acid-producing lactate. These findings open up opportunities for utilizing local LAB from tempoyak as functional agents in the development of safe, healthy, and economically valuable fermented food products. Thus, tempoyak not only has cultural value but also has significant scientific and commercial potential.

Nailla Indayani Sumardi; Ardi Mustakim

Konstanta : Jurnal Matematika dan Ilmu Pengetahuan Alam 2025 International Forum of Researchers and Lecturers

Mandai is a traditional fermented food made from the inner skin of the cempedak fruit (Artocarpus champeden) and is widely known in South Kalimantan. Its distinctive sour taste is a result of microbial activity during the natural fermentation process. This study highlights the important role of lactic acid bacteria (LAB) as the dominant agents in Mandai fermentation. The main objective of this research is to isolate and characterize LAB from fermented Mandai samples. The methods used in this study include serial dilution, inoculation on Nutrient Agar (NA) media, incubation at room temperature, colony morphology observation, and Gram staining using crystal violet. The results showed the presence of round colonies with a creamy white color and smooth surfaces, which indicate the characteristics of lactic acid bacteria. Most of the isolates showed Gram-positive properties, supporting their identification as lactic acid bacteria. This research not only enriches our understanding of the microbial ecology in Mandai fermentation but also opens up opportunities for utilizing LAB as a natural preservative or functional probiotic in both traditional and modern food products. The presence of LAB in Mandai fermentation suggests the potential to enhance the quality and health benefits of this traditional food, making it a viable source of probiotics beneficial for digestive health. Additionally, the use of LAB in Mandai processing could increase the appeal of this product in broader markets, both locally and internationally. Overall, this study provides a solid foundation for further development in utilizing microbes in traditional food fermentations. Moreover, it opens opportunities to improve the quality and sustainability of Mandai products while raising awareness about the importance of fermentation in enhancing the nutritional value and health benefits of traditional food products.

Zaskia Rahmawati; Ardi Mustakim

Pentagon : Jurnal Matematika dan Ilmu Pengetahuan Alam 2025 Asosiasi Riset Ilmu Matematika dan Sains Indonesia

Bekasam is a traditional fermented fish product commonly produced in South Sumatra and South Kalimantan, known for its distinctive sour taste resulting from the metabolic activity of lactic acid bacteria (LAB). LAB play a crucial role in the fermentation process, contributing to the product’s flavor, texture, and safety. This study aimed to isolate, identify, and characterize LAB from bekasam made using tilapia (Oreochromis niloticus). Isolation of bacterial strains was performed using the pour plate method on Nutrient Agar (NA) medium at various serial dilutions to obtain discrete colonies. The colonies were observed macroscopically to assess their morphological characteristics, including shape, size, color, surface texture, and edge profile. Further identification involved Gram staining to determine cell wall type and microscopic morphology. The results revealed that the bacterial colonies were generally small, round, white to cream in color, with smooth surfaces and flat edges. Gram staining demonstrated that the dominant isolates were Gram-positive bacteria, exhibiting rod-shaped or oval morphology. These characteristics are consistent with members of the genus Lactobacillus sp., a common LAB group in fermented fish products. Additional phenotypic traits observed included catalase-negative reactions, absence of spore formation, and the ability to produce lactic acid as the main metabolic end product. The combination of morphological, staining, and basic biochemical characteristics supports the initial identification of the isolates as LAB. The presence of Lactobacillus sp. in bekasam suggests their significant contribution to the fermentation process, influencing the product’s sourness, safety, and preservation. The findings of this study provide a basis for further molecular characterization and potential selection of LAB strains as starter cultures to improve the quality, consistency, and safety of bekasam in traditional and industrial production.

Rifky Rifaldo Munthe; Ardi Mustakim

Pentagon : Jurnal Matematika dan Ilmu Pengetahuan Alam 2025 Asosiasi Riset Ilmu Matematika dan Sains Indonesia

Bekasam, a traditional Indonesian fermented fish product, is produced through a natural fermentation process involving complex microbial communities, particularly fermentative bacteria. These microorganisms play a vital role in developing the product’s characteristic flavor, aroma, and texture. This study aimed to identify the morphological characteristics of fermentative bacteria present in bekasam made from Nile tilapia (Oreochromis niloticus) using direct microscopic observation without staining. Fresh samples of bekasam were collected and subjected to serial dilution before being cultured on nutrient agar (NA) medium. The cultures were incubated at room temperature for 24–48 hours to allow colony formation. Morphological examination was carried out by observing the colonies directly under a light microscope to assess cell shape, size, and arrangement. The results demonstrated a diversity of bacterial morphologies, with the majority consisting of rod-shaped (bacillus) and spherical (coccus) forms. Bacillus cells were often observed singly or in short chains, whereas coccus cells appeared either as single units, in pairs (diplococci), or forming short chains (streptococci). The morphological diversity observed suggests that multiple bacterial species may be involved in the fermentation process, each contributing differently to biochemical transformations such as protein degradation, lactic acid production, and flavor compound formation. Although morphological characterization alone cannot provide definitive bacterial identification, these findings serve as preliminary data for subsequent microbiological and molecular analyses. Further research using biochemical tests and molecular approaches, such as 16S rRNA gene sequencing, is recommended to confirm species identity and to explore their specific roles in fermentation. Understanding the microbial composition of bekasam can provide valuable insights for optimizing fermentation conditions, improving product consistency, and ensuring safety in traditional fish-based fermented foods.

Putri Rahmadani; Ardi Mustakim

Pentagon : Jurnal Matematika dan Ilmu Pengetahuan Alam 2025 Asosiasi Riset Ilmu Matematika dan Sains Indonesia

Fermentation of ikan bekasam is a traditional process that involves the activity of microorganisms to produce a product with distinct taste, aroma, and texture. This process includes various microorganisms, such as bacteria, fungi, and yeasts, that play a crucial role in the transformation of fish properties during fermentation. This study aims to isolate, identify, and characterize the microorganisms involved in the fermentation of ikan bekasam using Gram staining and specific staining techniques. The isolation process was carried out using selective media to grow microorganisms from the bekasam samples, including both the fish and the fermentation liquid. Microorganism identification was performed by observing colony morphology, microscopic characteristics, and biochemical reactions. Gram staining was used to differentiate between Gram-positive and Gram-negative bacteria, while specific staining techniques were employed to detect certain cellular structures that aid in the identification of microorganisms. The characterization of microorganisms included an analysis of their physiological and biochemical properties, such as their ability to produce enzymes or secondary metabolites that play a role in fermentation and the development of the characteristic bekasam flavor. The results of the study showed that ikan bekasam contains a variety of microorganisms, including lactic acid bacteria such as Lactobacillus spp. and Pediococcus spp., which play a primary role in fermentation by providing the characteristic sourness. In addition, yeasts such as Saccharomyces cerevisiae were found in the bekasam samples, contributing to the fermentation process. Fungi, such as Aspergillus spp., were also isolated, although their role in the fermentation of ikan bekasam is more limited. This study provides insights into the diversity of microorganisms involved in the fermentation of ikan bekasam and their potential applications in the food industry. These findings open up opportunities to optimize the fermentation process by utilizing these microorganisms to produce more consistent and high-quality bekasam. Future research could further explore the optimal conditions for improving the quality of this fermented product.

Muhammad Alvito Faros; Riri Murniati; Agus Hadi Santosa Wargadipura

Journal of New Trends in Sciences 2025 CV. Aksara Global Akademia

This research explores the engineering and performance evaluation of 17-4 PH stainless steel as a potential material for turbine blades in geothermal power plants (PLTP). To promote renewable energy innovation in industrial engineering, this study focuses on improving material reliability through microstructural optimization and mechanical property control. The material was produced using the investment casting method at PT SPVMB and then subjected to four heat treatment variations: H900, H1025, AVG (average), and as-cast conditions, with reference to ASTM A747 standards. Mechanical and corrosion characterization were performed through hardness and tensile tests, electrochemical corrosion analysis using geothermal water from the Dieng PLTP, and microstructural observation using an optical microscope. The results showed that the H900 condition had the highest hardness and yield strength (48.46 HRC and 939.25 MPa), but its corrosion rate was relatively high. In contrast, the H1025 heat treatment provides balanced mechanical strength (43.88 HRC and 860.91 MPa) with the lowest corrosion rate (0.027 mm/year), supported by a uniform tempered martensite structure. These findings indicate that heat treatment optimization significantly improves the suitability of 17-4 PH stainless steel for sustainable geothermal applications. The H1025 condition meets all the requirements for geothermal turbine blades, including hardness, strength, and corrosion resistance, potentially extending component life and reducing maintenance costs. Furthermore, the results of this study strengthen the agenda for developing durable, environmentally friendly materials to support renewable energy systems. This study also provides practical insights for industry in selecting the optimal heat treatment that combines mechanical performance and corrosion resistance in extreme geothermal environments.