Publication Search

66,773 articles from 556 journals · 1,699 citations tracked

Showing 21-40 of 115

Analytics

Kamariah Kamariah; Rosalia Putri; Dian Rahayu

Jurnal Riset Rumpun Ilmu Kesehatan 2026 Pusat riset dan Inovasi Nasional

The prevalence of correct handwashing behavior with soap in Aceh Province is 36%. School-age children are the time to instill PHBS values ​​and have the potential to be agents of change to promote PHBS both in the school environment, family, and society. The impact of lazy handwashing with soap can cause various diseases such as diarrhea, flu, hepatitis A, and impetigo (a contagious infection that usually occurs in children who rarely wash their hands, this disease is characterized by red skin which then develops into small blisters). The purpose of this study is to determine the effectiveness of mini posters as a health promotion media on handwashing behavior with soap in elementary school students. The research method is one group pretest posttest design. The sampling technique is total sampling, while the number of samples in this study was 80 students from grades 5 and 6 of elementary school. The results of the study obtained before the intervention of mini posters washing hands with soap behavior of elementary school students 77.5% of students did not wash their hands with soap after urinating and defecating. After the intervention of mini posters washing hands with soap behavior of elementary school students 87.5% did wash their hands with soap. There is an effect of mini poster intervention on increasing handwashing behavior in elementary school students with a p value of 0.000 < 0.05. The results show that mini posters are a reference for the effectiveness of health promotion in preventing infectious diseases in elementary school children by implementing a handwashing program with soap in the school environment

Wulan Ariby; Gistran Yoga Arinda; Fadli Hasbi Hasibuan

Inovasi Kesehatan Global 2026 Lembaga Pengembangan Kinerja Dosen

This study aims to describe the condition of clean water access and its impact on public health in Swadaya Village. The research employed a qualitative approach through in-depth interviews with household heads, community leaders, health workers, and local water managers. The findings reveal that access to clean water in Swadaya Village remains very limited. Most residents rely on dug wells and river water for daily needs, while only a small portion use water from the local water utility (PDAM) due to high installation costs and limited pipeline coverage. The water quality often decreases during the dry season, becoming cloudy, odorous, and causing skin irritation. These conditions contribute to the increasing incidence of waterborne diseases such as skin infections, diarrhea, and typhoid fever. Besides infrastructure limitations, the lack of community awareness regarding hygiene and sanitation practices worsens the situation. Although the village government has proposed clean water development programs, their implementation has been hampered by budget constraints. The study concludes that clean water problems in Swadaya Village are not only related to resource availability but also to environmental management and community behavior. Therefore, improving water infrastructure must be accompanied by community education to promote a healthy, safe, and sustainable living environment.

Susiani Susiani; Tri Surya Ayu Lestari; Hera Hera; Ismatul Auliyah; Nur Azila Putri

ARDHI : Jurnal Pengabdian Dalam Negri 2025 Asosiasi Riset Pendidikan Agama dan Filsafat Indonesia

The age of 4-6 years is an age that is vulnerable to disease because it is at this age that children's motoric starts to be active. Without realizing it, what children do is often close to germs that can cause diseases such as flu, cough, diarrhea. Hand washing is the process of mechanically removing dirt and dust from the skin surface and reducing the number of microorganisms. The purpose of hand washing is to eliminate the number of microorganisms. This activity is carried out through the means of joint learning activities in the form of face-to-face in groups, given counseling, including: material and practice of wet hand washing with running water and soap and dry hand washing with a hand sanitaizer. The results of this activity were analyzed using a fun learning method. The data used is numerical data from the observation of hand washing skills checklist. From the test results there is an effect of providing counseling on hand washing techniques on the hand washing skills of kindergarten students. The habit of washing hands using soap from an early age, it is hoped that later they will become a generation that is aware of the importance of cleanliness, not only personal hygiene, but also environmental hygiene. Not only that, with this simple thing that is done regularly and purposefully, children will later learn to do other habits correctly. One way to prevent children from getting sick is by washing hands. This handwashing habit should be instilled since.

Hermawati Hamalding; Angelika Maharani

Jurnal Sains dan Kesehatan (JUSIKA) 2025 Universitas Muhamadiyah Manado

Diarrhea is one of the diseases that remains a leading cause of morbidity and mortality among elementary school-aged children. A mother's knowledge as the primary caregiver is an important factor in the prevention of diarrhea. This study aims to analyze the effect of health education through pop-up book media on mothers' knowledge about diarrhea prevention in children at SDN 160 Mulyorejo II, Luwu Utara Regency. The research design used a quantitative quasi-experimental approach with a one group pretest posttest design. The population consisted of 143 mothers with children attending SDN 160 Mulyorejo II, with a sample of 54 respondents selected using stratified random sampling. The intervention was carried out by providing education using pop-up book media, followed by measuring knowledge before and after the intervention using a questionnaire. Data analysis using the Wilcoxon Signed Rank Test yielded p < 0.001, indicating a significant effect of health education through pop-up book media on mothers' knowledge about diarrhea prevention. This media is effective because it combines three-dimensional visual and interactive elements that enhance mothers' interest and understanding of health material. The conclusion is that health education through pop-up book media can significantly improve mothers' knowledge about diarrhea prevention in children, and is recommended as an alternative educational medium in public health promotion.

Erni Susanty Tahir; Fatika Puteri Rosyi; Puput Mulyono

Jurnal Pelayanan Masyarakat 2025 Lembaga Pengembangan Kinerja Dosen

Schools serve as environments where children receive a formal education. In addition to serving as educational institutions, schools can also pose a risk for spreading diseases. Diarrhea accounts for up to 65% of deaths, while other illnesses contribute to 26%. Healthy and clean living practices involve initiatives to offer learning experiences or establish environments for individuals, families, groups, and communities by facilitating communication, sharing information, and arranging educational opportunities to enhance knowledge, attitudes, and actions. This community service seeks to impart knowledge and awareness of clean and healthy living habits to Sekolah Dsar children, enabling them to incorporate these practices into their everyday lives. This community service is conducted through counseling delivered via lectures, interactive discussions, and simulations utilizing LCD media and instructional materials. The event was successful, and everyone involved was excited about this service project.

Azkiyyatu Zahra; Lilis Lismayanti

Jurnal Ilmu Kesehatan Umum, Psikolog, Keperawatan dan Kebidanan 2025 Asosiasi Riset Ilmu Kesehatan Indonesia

 Communicable diseases remain one of the leading public health burdens in Indonesia, particularly in densely populated residential areas and communities with inadequate sanitation. Poor housing conditions including insufficient ventilation, overcrowding, excessive humidity, inadequate lighting, and improper waste and wastewater management have been shown to increase the risk of respiratory infections, diarrhea, and other environment-related diseases. This literature review aims to identify effective environmental health strategies for preventing communicable diseases by synthesizing findings from 11 scientific articles published between 2014 and 2025. The review shows that basic sanitation, indoor air quality, waste management, and the implementation of the Community-Based Total Sanitation (STBM) program are key determinants in reducing disease incidence. Additionally, successful interventions depend heavily on clean and healthy living behaviors (PHBS) and active community participation. These findings highlight the importance of a comprehensive approach that integrates physical environmental improvements, health education, and community empowerment to create healthy settlements and sustainably reduce the risk of communicable diseases.

Sumarni Sumarni; Kusumastuti Kusumastuti; Siti Mutoharoh; Dina Saskia Budiarti; Enjel Laksana P +4 more

Karya Nyata : Jurnal Pengabdian kepada Masyarakat 2025 Lembaga Pengembangan Kinerja Dosen

Bloating in infants can also accompany other digestive disorders, such as vomiting, diarrhea, abdominal pain, and constipation. Babies with gas often vomit easily. Babies with diarrhea will have low potassium levels, resulting in bloating. One benefit of infant massage is addressing complaints of bloating and colic in infants. Tummy massage is a gentle abdominal massage designed to encourage trapped air to move. The goal is to move gas and other materials from the intestines to the large intestine. The method used was preparation through coordination with midwives and cadres in Selokerto Village, Sempor District, Kebumen Regency. The activity was implemented through lectures and tummy massage simulations. While evaluation and follow-up plans for the activity were carried out by evaluating the knowledge of mothers of toddlers and can be implemented independently using leaflets distributed to mothers of toddlers. The number of respondents in this activity was 10 mothers of toddlers. The activity went smoothly, all mothers enthusiastically participated in the activity. Respondents were aged between 20-34 years. The level of knowledge of respondents before the activity was that most had sufficient knowledge about bloating/colic in toddlers and tummy massage. Meanwhile, after the knowledge activity, most responded well. Conclusion: The community service activity was effective in increasing respondents' knowledge about gas/colic in toddlers and tummy massage.

Nur Hafizah Putri; Ditte Ayu Suntara

Jurnal Siti Rufaidah 2025 PPNI UNIMMAN

Diarrhea is the third leading cause of death in children. WHO estimates that the prevalence of diarrhea globally is almost 1.7 billion cases of diarrhea in children each year, in Indonesia the prevalence of diarrhea is 4.55% of cases. The incidence of diarrhea is caused by several factors, one of which is consuming street food. This study aims to determine the relationship between snack consumption and the incidence of diarrhea in students at Elementary School 008 Batam City, Baloi Permai Village in 2024. The design of this study used cross-sectional. This study was conducted on students in grades IV, V, and VI totaling 50 students. The sampling technique used non-probability sampling with purposive sampling technique. The measuring instrument in this study was a questionnaire on snack food consumption and diarrhea incidence. The results of the study showed that the incidence of diarrhea in students with the category of always consuming snack foods was 4 students (33.3%), often consuming snack foods was 14 students (87.5%), sometimes consuming snack foods was 7 students (53.8%), and never consuming snack foods was 3 students (33.3%). Based on the results of the Chi-Square Test statistic using SPSS, the probability value (p) was smaller than alpha (0.013)

Achmad Faris Fadhlullah; Dika Arif Sihombing; Rizki Riandi; Suri Handayani

Jurnal Sistem Informasi dan Ilmu Komputer 2025 International Forum of Researchers and Lecturers

Toddlers are a vulnerable age group to various types of diseases due to their immune systems that are still developing. Limited utilization of medical record data and the lack of structured information regarding disease patterns in toddlers based on age and causative factors have resulted in suboptimal prevention and treatment efforts. Therefore, an approach is needed to systematically classify toddler disease data. This study aims to apply data mining techniques using the clustering method with the K-Means algorithm to group types of diseases in toddlers based on age and causative factors. The variables used in this study include toddler age, type of disease, and causative factors. The data were obtained from RSUD Dr. R. M. Djoelham Binjai and processed using MATLAB software with three clusters. The results show that the K-Means algorithm successfully groups toddler disease data into three clusters with different characteristics. The first cluster is dominated by toddlers aged 0–11 months with appendicitis caused by genetic factors. The second cluster is dominated by toddlers aged 1–3 years with diarrhea caused by environmental factors and has the largest number of members. Meanwhile, the third cluster is dominated by toddlers aged 0–11 months with sore throat caused by environmental factors. The clustering results indicate a relationship between toddler age, disease type, and causative factors, which can be used as supporting information for decision-making in the prevention and treatment of toddler diseases.

Alisyah Pratiwi; Hartalina Mufidah; Rian Anggia Destiawan

Jurnal Ilmu Kesehatan 2025 Lembaga Pengembangan Kinerja Dosen

Water plays a fundamental role in sustaining human life, particularly as a source of drinking water. Nonetheless, the consumption of water that does not comply with established health standards can result in illnesses such as diarrhea and vomiting. In the Patrang District, the use of refillable drinking water has gained popularity as an economical substitute for bottled water. Despite its convenience, there are growing concerns about the microbiological safety of refillable drinking water, especially regarding contamination by Coliform and Escherichia coli bacteria, which serve as key indicators of water hygiene and safety. The objective of this research was to determine the contamination levels of Coliform and Escherichia coli bacteria in refillable drinking water. The study utilized a quantitative research design with descriptive analysis and employed the Most Probable Number (MPN) method for testing. Data collection took place from May to June 2024, comprising five water samples labeled X, Y, Z, A, and B, obtained from refillable drinking water depots in Patrang District using a simple random sampling approach. The results indicated that out of the five samples tested, the sample labeled Z was positive for Coliform with an MPN value of 920 cfu/mL, while the sample labeled A was positive for both Coliform and Escherichia coli with an MPN value of 2,000 cfu/mL. From these findings, two samples (Z and A) were identified as contaminated, with the highest bacterial concentration found in sample A, which had an MPN value of 2,000 cfu/mL

Arnisona Tumiar Br Sinaga; Nelli Roza; Huzaima Huzaima

Jurnal Riset Rumpun Ilmu Kedokteran 2025 Pusat riset dan Inovasi Nasional

Diarrhea is a leading cause of morbidity and mortality in children under five, particularly in developing countries like Indonesia. To reduce the incidence of diarrhea, the government has implemented a policy to prevent it by administering rotavirus vaccination. To determine the relationship between rotavirus vaccination and the incidence of diarrhea in infants in Sadai sub-district, the working area of UPT Puskesmas Sei Panas, Batam City in 2024. This study was a quantitative study with a cross-sectional approach to 72 infants who received rotavirus vaccination.The implementation time starts on October 23 to November 06, 2024. Sampling using cluster random sampling technique. Measurement tools using questionnaires and observation sheets. Analysis of the data used is the chi square test. The study obtained rotavirus vaccination as many as 40 infants (55.6%), the incidence of moderate diarrhea as many as 37 infants (51.4%). Chi-Square test results showed the value of p value = 2.167. Because of the value (p value 2.167 > 0.05), this relationship is not statistically significant. There is no significant relationship between rotavirus vaccination and the incidence of diarrhea in infants in Sadai Village working area of UPT Puskesmas Sei Panas Batam City in 2024.

Jennifer Chriseis Caecilia Tangkilisan; Wury Damayantie; Warrantia Citta Citti Putri

DIAGNOSA: Jurnal Ilmu Kesehatan dan Keperawatan 2025 International Forum of Researchers and Lecturers

Bajakah stem (Spatholobus littoralis Hassk) is one of the plants often used as traditional medicine. This plant is often used by the people of the interior of Kalimantan as a traditional medicine. The Dayak people know the bajakah stem plant as a medicine to increase stamina and cure various diseases, boiled water from bajakah stems can be used to cure diarrhea, dysentery, aches and pains, wounds, and other diseases and is even considered to cure cancer. This study aims to identify saponin compounds in ethanol extracts and n-hexane fractions of bajakah tampala stems (Spatholobus littoralis Hassk) using the Thin Layer Chromatography (TLC) method. From the results of this study, it can be concluded that the thin layer chromatography profile shows that the ethanol fraction produces seven spots with varying Rf values ​​(0.93; 0.80; 0.77; 0.75; 0.68; 0.63; and 0.56). Meanwhile, the n-hexane fraction only produced three spots with narrower Rf values ​​(0.56, 0.68, and 0.63). The comparison compound, sapogenin, had an Rf value of 0.98. This study concluded that ethanol was more effective than n-hexane in extracting bioactive compounds, especially saponins

Jessya Intan Panggabean; Wury Damayantie; Warrantia Citta Citti Putri

DIAGNOSA: Jurnal Ilmu Kesehatan dan Keperawatan 2025 International Forum of Researchers and Lecturers

Bajakah tampala (Spatholobus littoralis Hassk.) is a medicinal plant that is traditionally used by the Dayak people to overcome various diseases, including indigestion such as diarrhea and dysentery. This plant is known to contain secondary metabolites, including flavonoids, tannins, saponins, alkaloids, and phenolics, which have the potential to have antibacterial activity. This study aims to evaluate the antibacterial activity of pulp extract of patchy bajakah with n-hexane solvent against Escherichia coli ATCC 25922. The extract was obtained through the maceration method and tested using the well diffusion method at concentrations of 10%, 20%, and 30%. The results showed a relatively low yield of the extract (0.10–0.14%) with a reddish-orange color. The inhibition zones formed ranged from 4.28–5.55 mm with significant differences between concentrations (p = 0.000), but the antibacterial activity was relatively low. These findings indicate that the active compounds of the patch stalk are likely to be more polar or semi-polar, so they are less optimally extracted using the nonpolar solvent n-hexane. Therefore, further research with different solvent variations, particularly those that are polar, is urgently needed to uncover the greater antibacterial potential of these plants.

Swastika Oktavia; Ayu Febi Lestari; Arini Khaerunnisa

International Journal of Medicine and Health 2025 Lembaga Pengembangan Kinerja Dosen

Leaves of Etlingera walang (Blume) RMSm have been empirically used in traditional medicine to relieve stomach disorders and are known to contain secondary metabolites with potential antimicrobial properties. Salmonella typhi infection can cause typhoid fever with symptoms such as diarrhea and abdominal pain, while Candida tropicalis is a fungus that can infect the gastrointestinal tract and lead to digestive disturbances. This study aimed to evaluate the antibacterial and antifungal activities of the ethanolic extract of E. walang leaves against S. typhi and C. tropicalis. The antimicrobial assay was carried out using the disk diffusion method at extract concentrations of 25%, 50%, and 100%, with chloramphenicol and ketoconazole as positive controls, and DMSO as the negative control. Phytochemical screening revealed the presence of alkaloids, flavonoids, saponins, and tannins. However, the results showed that the extract did not produce inhibition zones against either test microorganism. Extract evaluation indicated a moisture content of 12.23%, total ash 13.39% (above the standard), acid-insoluble ash 1.25%, total plate count 1.8 × 10 ⁴ , and mold and yeast count 1.0 × 10 ³. The findings suggest that although the ethanolic extract of E. walang leaves contains secondary metabolites and is traditionally used for stomach ailments, it does not exhibit antimicrobial activity against S. typhi or C. tropicalis.

Tri Wahyuti; Indra Tri Astuti; Nopi Nur Khasanah

Jurnal Mahasiswa Ilmu Kesehatan 2025 STIKes Ibnu Sina Ajibarang

Diarrhea remains a major public health problem among children in Indonesia, with a high prevalence that impacts morbidity, mortality, and child development. One of the key risk factors is non-compliance with hygiene practices, particularly handwashing behavior. This study aimed to determine the relationship between handwashing compliance and diarrhea cases among children treated in the Pediatric Ward of Dahlia, RSUD Dr. H. Soewondo Kendal. The research employed a quantitative analytic method with a cross-sectional design, involving a total sampling of 39 child respondents aged 5–10 years who met the inclusion criteria. Data were collected using a handwashing compliance questionnaire and a diarrhea incidence questionnaire, and analyzed using Spearman’s Rank Correlation test. The results showed that most respondents were in the moderately compliant (53.8%) and compliant (43.6%) categories for handwashing, while the incidence of diarrhea remained relatively high at 51.3%. Bivariate analysis indicated a significant negative relationship between handwashing compliance and diarrhea cases (ρ = -0.538; p = 0.000), meaning that the higher the level of handwashing compliance, the lower the incidence of diarrhea. In conclusion, handwashing compliance is an important protective factor in reducing the risk of diarrhea among children. These findings emphasize the need for continuous education for children and parents regarding proper handwashing practices, as well as the integration of Clean and Healthy Living Behavior (PHBS) promotion into healthcare and educational services to prevent diarrhea from an early age

Bintang Wicaksana; Novriyenni Novriyenni; Suci Ramadani

Neptunus: Jurnal Ilmu Komputer Dan Teknologi Informasi 2025 Asosiasi Riset Teknik Elektro dan Informatika Indonesia

Typhoid fever is a significant health issue caused by the Salmonella Typhi bacteria, leading to symptoms such as fever, abdominal pain, diarrhea, muscle pain, and serious complications if not treated promptly. A common challenge faced by society is limited access to medical professionals, especially in remote areas, and delays in recognizing symptoms. To address this problem, this study designs and implements a web-based expert system using the Certainty Factor (CF) method, which helps diagnose typhoid fever quickly and accurately. The Certainty Factor method is used to calculate the certainty level of the symptoms experienced by the patient, providing a diagnosis result in the form of early-stage typhoid, mild typhoid, or severe typhoid. The system was developed using PHP programming language and MySQL database, and tested at RSUD Djoelham Binjai City. The research data was obtained from patients at RSUD Djoelham Binjai with a case study on patient number 22. The processing of symptoms through Certainty Factor calculation showed that the patient is most likely to have severe typhoid with a certainty value of 0.9443 or 94.43%. This result proves that the Certainty Factor method can be used to assist in providing an accurate early diagnosis of typhoid fever with a high degree of accuracy.

Nur Arifatus Sholihah; Asri Reni handayani; Herni Hasifah; Hamdin Hamdin

ARDHI : Jurnal Pengabdian Dalam Negri 2025 Asosiasi Riset Pendidikan Agama dan Filsafat Indonesia

The low level of understanding among elementary school students regarding common diseases and their prevention can increase the risk of both communicable and non-communicable diseases from an early age. Therefore, effective health education efforts are essential to instill healthy habits in children. This community service activity aimed to improve the knowledge of elementary school students in the working area of Labuhan Sumbawa Public Health Center about common diseases and preventive measures through an interactive educational approach. The methods used included health counseling with visual media, engaging educational games, and small group discussions to encourage active participation. The activity was conducted in June 2025, involving 30 students from two elementary schools selected purposively. The educational content focused on dengue fever, diarrhea, influenza, helminthiasis, and acute respiratory infections (ARI), along with prevention strategies such as maintaining personal hygiene, consuming nutritious food, and the importance of immunization. Evaluation was carried out through pre-test and post-test assessments to measure the improvement in students’ understanding. The results showed a significant increase in students’ knowledge of the delivered material. Additionally, students demonstrated high enthusiasm and active involvement during the sessions, particularly in the educational games. These findings indicate that an interactive educational approach is effective in enhancing students’ understanding of disease prevention. This program is expected to serve as a model for sustainable health education in other elementary schools to prevent diseases from an early age and support the implementation of clean and healthy living behaviors within the school environment

Nurcahyani Nurcahyani; Ananda Pratama; Febryanti Aryanda; Riska Rahayu; Aiy Rani +13 more

Jurnal Pengabdian Masyarakat 2025 Lembaga Pengembangan Kinerja Dosen

Maintaining health is important for every individual, because by maintaining health, it can prevent susceptibility to disease. One important effort to get used to is maintaining hand and mouth hygiene. Washing hands with soap is the process of removing dirt and dust from both hands using water and soap, which can prevent various diseases such as diarrhea and ARI. While brushing teeth is the process of cleaning teeth from food residue stuck in the teeth and oral cavity, the benefit of getting used to brushing teeth is to prevent tooth decay. The importance of awareness about personal hygiene must be instilled from an early age, starting from this, KKN University Karimun students, and then conducted socialization activities at State Elementary School 004 and State Elementary School 007, Belat District. This socialization activity used simple lecture methods, direct practice, educational games, and audio-visual media. From the results of the socialization activities that have been carried out, it has been successful in providing an understanding of the importance of maintaining personal hygiene through washing hands and brushing teeth. With the education that has been provided, it is hoped that it can have a positive impact on student behavior in maintaining personal hygiene.

Nurcahyani Nurcahyani; Ananda Pratama; Febryanti Aryanda; Riska Rahayu; Aiy Rani +13 more

Jurnal Pengabdian Masyarakat 2025 Lembaga Pengembangan Kinerja Dosen

Maintaining health is important for every individual, because by maintaining health, it can prevent susceptibility to disease. One important effort to get used to is maintaining hand and mouth hygiene. Washing hands with soap is the process of removing dirt and dust from both hands using water and soap, which can prevent various diseases such as diarrhea and ARI. While brushing teeth is the process of cleaning teeth from food residue stuck in the teeth and oral cavity, the benefit of getting used to brushing teeth is to prevent tooth decay. The importance of awareness about personal hygiene must be instilled from an early age, starting from this, KKN University Karimun students, and then conducted socialization activities at State Elementary School 004 and State Elementary School 007, Belat District. This socialization activity used simple lecture methods, direct practice, educational games, and audio-visual media. From the results of the socialization activities that have been carried out, it has been successful in providing an understanding of the importance of maintaining personal hygiene through washing hands and brushing teeth. With the education that has been provided, it is hoped that it can have a positive impact on student behavior in maintaining personal hygiene.

Indri Artanti; Ardi Mustakim

Algoritma : Jurnal Matematika, Ilmu pengetahuan Alam, Kebumian dan Angkasa 2025 Asosiasi Riset Ilmu Matematika dan Sains Indonesia

Batang Bungo River faces severe pollution from domestic waste and illegal gold mining, which has led to an increase in skin diseases and diarrhea among residents of Tanjung Gedang, exacerbated by poor physical-chemical water quality, including low pH and high levels of Total Suspended Solids (TSS) and Chemical Oxygen Demand (COD), all of which foster the growth of pathogenic microorganisme. This study aimed to identify bacteria and fungi present in Batang Bungo River water, characterizing their colony morphology and microscopic structures to understand the impact of pollution on microbial communities. The methodology involved serial dilution of water samples, followed by inoculation onto Nutrient Agar (NA) media using the pour plate technique, and incubation at 37°C for 24-48 hours. Macroscopic observations of colonies (color, shape, texture) were performed, and representative colonies were stained with crystal violet for microscopic observation at 1000x magnification to identify cellular and hyphal structures. The results indicated the presence of various microorganisms, including Gram-positive bacteria, filamentous fungi, and possibly protozoa, with colonies exhibiting characteristics such as off-white color, rough surfaces, and irregular edges. Microscopic examination after crystal violet staining revealed rod-shaped (bacilli), spherical (cocci) structures, and branched filamentous structures resembling hyphae, consistent with a mixture of bacteria and filamentous fungi. The identification of pathogens like Clostridium, Dermatophilus, and Escherichia coli in previous studies, coupled with the poor water quality, confirms significant microbiological and chemical contamination. Crystal violet proved effective as a stain for microscopic identification of microorganism structures. In conclusion, the water quality of Batang Bungo River is highly concerning and requires serious attention for monitoring and management to safeguard public health and the river ecosystem.