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Batang Bungo River faces severe pollution from domestic waste and illegal gold mining, which has led to an increase in skin diseases and diarrhea among residents of Tanjung Gedang, exacerbated by poor physical-chemical water quality, including low pH and high levels of Total Suspended Solids (TSS) and Chemical Oxygen Demand (COD), all of which foster the growth of pathogenic microorganisme. This study aimed to identify bacteria and fungi present in Batang Bungo River water, characterizing their colony morphology and microscopic structures to understand the impact of pollution on microbial communities. The methodology involved serial dilution of water samples, followed by inoculation onto Nutrient Agar (NA) media using the pour plate technique, and incubation at 37°C for 24-48 hours. Macroscopic observations of colonies (color, shape, texture) were performed, and representative colonies were stained with crystal violet for microscopic observation at 1000x magnification to identify cellular and hyphal structures. The results indicated the presence of various microorganisms, including Gram-positive bacteria, filamentous fungi, and possibly protozoa, with colonies exhibiting characteristics such as off-white color, rough surfaces, and irregular edges. Microscopic examination after crystal violet staining revealed rod-shaped (bacilli), spherical (cocci) structures, and branched filamentous structures resembling hyphae, consistent with a mixture of bacteria and filamentous fungi. The identification of pathogens like Clostridium, Dermatophilus, and Escherichia coli in previous studies, coupled with the poor water quality, confirms significant microbiological and chemical contamination. Crystal violet proved effective as a stain for microscopic identification of microorganism structures. In conclusion, the water quality of Batang Bungo River is highly concerning and requires serious attention for monitoring and management to safeguard public health and the river ecosystem.  

Pogalan Village in Pakis District, Central Java, is known as a horticultural agricultural center, with abundant vegetable production every year. However, behind this potential, this village faces a serious problem in the form of large amounts of organic waste generated from harvest residue and agricultural processing. Waste that is not managed properly has the potential to cause environmental pollution, reduce the village's aesthetics, and become a source of disease spread. To address these problems, the Tidar University Community Service Program (KKN-50) Team conducted a socialization activity for Black Soldier Fly (BSF) maggot cultivation as an environmentally friendly and economically valuable organic waste management solution. BSF maggots have been proven to decompose organic waste by up to 60% in a short time and produce useful by-products, such as dried maggots as high-protein animal feed and kasgot (used maggots) that can be used as organic fertilizer. The activity was held on July 31, 2025, at the Pogalan Village Hall, with the main participants being PKK mothers as agents of change at the household and community levels. The implementation method included initial observation of the village's potential and problems, creation of a maggot cultivation prototype, theoretical material outreach, and live demonstrations of the cultivation process. The material provided covered the BSF life cycle, cultivation techniques, selection of growing media, maggot population management, and utilization of cultivation products. The results of the activity showed high enthusiasm from participants, as reflected in the many questions and discussions during the training session, as well as their increased understanding of the benefits and techniques of maggot cultivation.

This study aims to analyze the quality of domestic wastewater based on three main parameters: pH, ammonia, and Chemical Oxygen Demand (COD). The wastewater samples were collected from an industrial area, chosen for its role as a primary source of domestic waste that can impact environmental quality. The pH was tested using potentiometry, while ammonia and COD concentrations were measured using UV-Vis spectrophotometry, a precise method for analyzing these substances. The results showed that the pH values of the wastewater ranged from 6.68 to 10.28. This variation in pH reflects the acidity or alkalinity of the wastewater, with some samples being neutral to slightly alkaline. The ammonia levels detected ranged from 0.0654 to 0.3849 mg/L. While these levels are within the limits set by regulations, they indicate a significant presence of ammonia in the wastewater, which requires continued monitoring. However, the most striking finding was the COD levels, which ranged from 21.60 to 245.71 mg/L, with some samples showing very high values. High COD levels indicate a large presence of organic matter, which can lead to a decline in water quality. Some samples with COD values exceeding the threshold of domestic wastewater quality standards, as stated in Ministerial Regulation No. 68 of 2016, require additional treatment to reduce organic contamination. Overall, while the pH and ammonia values remain within safe limits, the high COD levels in some samples indicate that further treatment is necessary to reduce organic pollution. This study emphasizes the importance of routine monitoring of domestic wastewater to protect environmental quality and prevent broader negative impacts on aquatic ecosystems and human health.  

This study aims to analyze the influence of domestic wastewater management and space utilization control on pests in river-equivalent areas in Lalat Regency. The main problem raised is the low effectiveness of environmental management in the corresponding area due to the increase in domestic activities and its weakness in spatial planning. The approach used is quantitative with multiple linear regression methods. Primary data were obtained through questionnaires to 200 respondents, and secondary data were obtained through documentation studies and field observations. The results of the analysis showed that simultaneously, the five independent variables (environmental management, space availability, community participation, government supervision, and environmental infrastructure) had a significant effect on the habitat in the corresponding area (p = 0.046). Partially, only waste management (p = 0.016) and community participation (p = 0.042) had a significant effect. A determination coefficient (R²) value of 7.5% indicates that the desires of a region are also influenced by other external factors. Based on these findings, it is recommended that the need to improve the household waste management system through the construction of communal WWTP and improve the quality of household septic tanks, as well as community empowerment through the establishment of environmental forums and sustainable sanitation education to support spatial control and poverty in commensurate areas.

Waste management is a major challenge in sustainable development, particularly in areas with tourism potential. Paya Jeget Village, Pegasing District, Central Aceh Regency, a developing tourist destination, is addressing waste management issues that require serious attention. The increasing number of tourists visiting the village has the potential to significantly increase the volume of waste generated. Without proper management, this condition can threaten environmental sustainability and public health. Therefore, a community-based waste management approach is needed that involves the active participation of all stakeholders, from village government, community leaders, tourism operators, and the general public. The objectives of this community service activity are to increase public knowledge and awareness about sustainable waste management, develop an integrated community-based waste management system, and establish independent community waste management groups to realize a clean and healthy tourist destination. Implementation methods include outreach and education, practical training, the formation of working groups, and ongoing mentoring to ensure the program runs consistently. The community service activities carried out on June 2, 2025 have achieved the following results: 45 participants participated in the outreach activities, 90% of participants understood the importance of waste from a religious and environmental perspective, the formation of collective awareness to maintain environmental cleanliness, the formation of a waste management group consisting of 15 members, the availability of waste sorting places at three strategic points, the implementation of waste sorting activities at the household level, and the start of organic waste composting activities. In addition, this activity also succeeded in fostering a spirit of mutual cooperation in waste management and improving residents' skills in processing organic waste into compost.

This community service activity was carried out in Dahu Village, Pandeglang Regency, with the main objective of introducing and implementing biopore infiltration hole technology as a simple and environmentally friendly solution to address waterlogging and organic waste management. Biopores are small-diameter vertical holes dug into the ground and then filled with organic waste, such as dry leaves, vegetable scraps, or food scraps. This organic waste will naturally decompose through a decomposition process, producing compost that is useful for fertilizing the soil. Furthermore, the presence of biopore holes can improve the groundwater absorption capacity, thereby reducing the risk of waterlogging and minor flooding around residential areas. During the implementation, we as students not only provided theoretical explanations through socialization sessions but also actively participated directly in the field. Together with residents, we practiced making biopore holes, starting from the stages of preparing tools and materials, the process of drilling the soil, and filling the holes with organic waste. This practical activity was designed to help the community understand the steps for making biopores independently and utilizing them sustainably. The results of the activity showed an increase in community understanding and awareness of the importance of easy, affordable, and beneficial waste management. The community is beginning to realize that waste processing does not always require large costs or complicated technology. Biopore holes have been proven effective in accelerating rainwater absorption, reducing household waste volume, and improving soil fertility. Beyond the environmental impact, this activity also strengthens relationships between students and villagers through the collaboration established during the biopore construction process. Thus, this program is expected to be the first step towards a cleaner, healthier, and more sustainable village.

Natural resource management in Paccellekang Village, which includes rice paddies, plantations, and teak trees, remains suboptimal despite its significant potential, particularly the abundant wood waste. This wood waste largely originates from agricultural activities, plantations, and small-scale wood processing. Currently, this waste is often simply burned or dumped, potentially polluting the environment and causing air pollution. However, if managed properly, wood waste can be transformed into high-value products that can provide financial benefits to the community. This community service activity aims to empower farmer groups through training in processing wood waste into marketable products such as charcoal, liquid smoke, and wood flour. These products not only have economic value but are also useful in various sectors, for example, liquid smoke as a natural preservative, charcoal as an environmentally friendly fuel, and wood flour as an industrial raw material. The program implementation method uses a structured approach that includes initial observations to identify potential partners and needs, outreach to provide a comprehensive understanding of the program's objectives, and training in wood waste processing technology. Furthermore, intensive mentoring and evaluation of implementation results are provided to ensure program sustainability. The results demonstrated that the farmer group members understood the concept of productive wood waste management and demonstrated high enthusiasm for developing innovative products. This program successfully improved the community's skills in utilizing local resources while reducing the negative impact of waste on the environment. Thus, this activity contributed to increasing community income and supporting sustainable economic development in Paccellekang Village.

Waste management remains a serious problem, particularly in rural areas, which generally have relatively low levels of environmental awareness. Inorganic waste such as plastic, glass, and aluminum is a major concern because it takes hundreds of years to decompose naturally. This situation poses potential long-term environmental hazards, including soil and water pollution, and ecosystem disruption. Therefore, effective educational efforts are needed to raise public awareness of waste issues. This community service activity was carried out in Sarah Perlak Village, Sungai Mas District, West Aceh Regency. The focus of the activity was the installation of educational signs containing information about the decomposition time of various types of waste. Implementation methods included direct observation to identify specific problems at the site, informal outreach to local residents, demonstrations related to waste sorting, and the creation and installation of educational signs. The signs were made from wooden planks and decorated with real waste such as plastic bottles, aluminum cans, and used paper, thus providing a tangible visualization to the community. The results of the activity showed that the visual approach through educational signs was able to attract the attention of residents, both children and adults. The information was presented clearly and accompanied by real-life examples, making the message easier to understand and remember. In addition, direct interaction through outreach and demonstrations provided an opportunity for residents to ask questions and share experiences related to waste management. Overall, this educational medium has proven effective in conveying environmental messages, raising awareness, and motivating the community to start sorting and reducing waste at the source. Going forward, this program is planned to be expanded through training in processing waste into useful products, thereby not only reducing pollution but also providing economic benefits to the community.

This research aims to design and build a web-based syar'i clothing inventory information system at Umama Gallery Yogyakarta. The background of this research is based on the problem of inventory management which has been done manually, which often causes obstacles such as delays in stock information, potential recording errors, wasted paper, and difficulties in preparing inventory reports quickly and accurately. To overcome these problems, a web-based information system was developed by referring to the Software Development Life Cycle (SDLC) Waterfall model, which includes the stages of planning, needs analysis, design, implementation, and testing. Research data was obtained through interviews with store managers and literature studies to support the analysis and design of the system. The implementation results show that the inventory information system built is able to simplify the process of recording and monitoring inventory in real-time, thereby helping to reduce the potential for recording errors and speeding up the reporting process. In addition, this system also reduces dependence on physical documents, making it more environmentally friendly and efficient in the use of resources. Available features include data management of goods, suppliers, users, incoming and outgoing goods transactions, and user account management. The integration of these features supports comprehensive inventory management, improves operational efficiency, and facilitates management in making accurate, data-driven decisions. With this system, Umama Gallery Yogyakarta has gained an effective, reliable, and integrated tool to support smooth operations and more modern and professional inventory management. Similar systems have the potential to be implemented in similar businesses to improve inventory management quality and competitiveness in the digital age.

Introduction the management of expired drugs is an important part of the hospital's pharmaceutical logistics system to ensure the safety of patients, staff, and to protect the environment. The purpose of this study is to evaluate the implementation of procedures for returning expired drugs to the manufacturer or pharmaceutical company, as well as to examine the expired drug disposal system, particularly the methods of direct disposal to the Final Disposal Site (TPA) and open burning at RSUD Mohammad Natsir Solok. However, the implementation in the field has not been entirely effective. The method used in this study is a qualitative descriptive approach, with data collection techniques including observation, interviews, and documentation. The approach involved several informants who were directly involved in pharmaceutical logistics management in order to obtain in-depth and relevant data. The results of the study show that the hospital has not carried out expired drug destruction using the methods of direct disposal to the TPA or open burning. No clear information or official documentation was found regarding the use of these two methods. Informants stated that even if such methods were used, they occurred in the past and were not formally documented. The conclusion of this study is that open burning and direct disposal to the TPA have not become part of the expired drug management system at RSUD Mohammad Natsir Solok. The recommendation of this research is the development of clear operational procedures and increased staff awareness regarding the importance of safe and standard-compliant pharmaceutical waste managemen

This research is motivated by the suboptimal strategy of the Pangandaran Regency Tourism and Culture Office in supporting sustainable tourism development. Several problems identified include the lack of optimal management of facilities and infrastructure, such as waste management, and inadequate transportation facilities. Furthermore, tourism promotion has not been carried out in a sustainable and measurable manner, compounded by the absence of a Regional Promotion Agency. Human resources (HR) in the tourism sector also still lack a comprehensive understanding of the concept of sustainable tourism, which is a major challenge in implementing this policy effectively. The purpose of this study was to determine the strategies implemented by the Pangandaran Regency Tourism and Culture Office in developing sustainable tourism. The method used was descriptive qualitative with data collection techniques through observation, interviews, and documentation. Informants in this study consisted of government elements, communities, and tourism business actors. The results showed that the strategies implemented by the Pangandaran Regency Tourism and Culture Office encompass four dimensions of strategic management: environmental monitoring, strategy formulation, strategy implementation, and evaluation and control. However, the strategies that have been implemented have not been optimal. Several efforts have been made, including increased training based on local potential, technology education, the formation of promotional groups, and regular activity evaluations. However, major challenges remain: strengthening coordination between various stakeholders and improving natural resource management and more environmentally friendly supporting facilities. This study illustrates the need for strategic and policy adjustments to optimize sustainable tourism development in Pangandaran Regency, with a focus on increasing understanding and involvement of the community and businesses, as well as optimizing infrastructure and promoting more planned and measurable initiatives.

Limited sanitation facilities among street vendors, particularly those selling meatballs, chicken noodles, and porridge, often pose a problem that impacts food safety. One common practice is repeatedly washing bowls and spoons with just a bucket of water, followed by drying the utensils with a cloth. This contaminated water can transmit pathogenic microorganisms that cause foodborne illnesses, increasing the risk of health problems for consumers. This problem requires a practical solution that street vendors can implement, taking into account limited space, mobility, and operational costs. This community service activity used a pre-experimental design method with a simple technological engineering approach to improve the sanitation of tableware. The innovation developed was a portable bowl washer made from used mineral water gallons, modified to be mobile, efficient, and water-efficient. The manufacturing process included design, assembly, testing, and evaluation with street vendors. Success was measured by observing the level of cleanliness of the tableware before and after use, as well as assessing the level of satisfaction and ease of use by the vendors. The results showed that the implementation of this portable sanitation device increased the effectiveness of tableware washing, reduced the repeated use of dirty water, and minimized the risk of cross-contamination. Partnering vendors stated that the device is practical, lightweight, and doesn't disrupt their sales activities. They hope this innovation will be replicated by other vendors and that local governments will consider implementing it as a standard for mobile food sanitation. This effort will not only improve the hygiene of eating utensils but also contribute to preventing foodborne illnesses in the community.

Electricity has emerged as an essential requirement in modern life. As demand escalates, electricity costs rise, making wastefulness a drain on financial resources. Consequently, forecasting electricity usage can enhance our management of consumption. This study presents an IoT-based monitoring and forecasting system for electricity consumption. The system comprises two NodeMCU micro-controllers, a PZEM-004T sensor for collecting real-time power data, and three relays that regulate the current flow to three distinct electrical appliances. The data gathered is transmitted to a web application utilizing the k-Nearest Neighbor (k-NN) algorithm to forecast future electricity usage based on historical patterns. We evaluated the system's performance using four weeks of electricity consumption data. The results indicated that predictions were most accurate when the user’s daily consumption pattern remained stable, achieving a Mean Absolute Error (MAE) of approximately 1 watt and a Mean Absolute Percentage Error (MAPE) ranging from 1% to 1.7%. Additionally, predictions were notably precise during the early morning hours (3:00 AM to 8:00 AM) when k=6 was employed. This study demonstrates the effectiveness of integrating IoT-based systems with machine learning for real-time energy monitoring and forecasting. Furthermore, it emphasizes the application of data mining techniques within embedded IoT environments, providing valuable insights into the implementation of lightweight machine learning for smart energy systems.

This study aims to examine the use of cattle waste as organic fertilizer to minimize agricultural operational costs in Mrawan Village, Tapen District, Bondowoso Regency. Cattle waste, particularly manure, is an abundant local resource that has not been optimally utilized by the local community. The majority of farmers in the village still rely on chemical fertilizers, which are relatively expensive and have a negative impact on long-term soil health. Therefore, this program is designed to provide a sustainable alternative solution through an educational approach and community empowerment. The methods used in this activity include outreach, technical training, and direct assistance in the process of making organic fertilizer from cow manure. Education focuses on simple fermentation techniques, the composition of natural additives, and appropriate fertilizer application methods. Farmers are actively involved in every stage of the activity, so they become not only beneficiaries but also agents of change in environmentally friendly agricultural practices. The results of the activity indicate that the use of organic fertilizer from cattle waste can reduce the cost of purchasing chemical fertilizers by up to 40% in a single planting season. In addition, organic fertilizer has been shown to increase soil fertility, improve soil structure, and support healthier plant growth. Environmental impacts are also reduced, as livestock waste management is more controlled and does not pollute water or air sources. Therefore, utilizing cattle waste as organic fertilizer not only reduces environmental pollution but also provides an economic and ecological solution that benefits local farmers. This program is expected to become a model for empowerment that can be replicated in other areas with similar characteristics.

Dengue Hemorrhagic Fever (DHF) remains a significant public health challenge in Indonesia, with the increasing prevalence of cases every year. One of the key methods of controlling the spread of DHF is to target the vector, Aedes aegypti mosquitoes, by eradicating their larvae. However, the widespread and continuous use of chemical larvicides has raised concerns regarding the development of mosquito resistance and environmental pollution. This community service activity aimed to provide students at Duta Karya Vocational High School with knowledge and practical skills to use natural, eco-friendly alternatives for controlling mosquito larvae, focusing on papaya seeds (Carica papaya) and alum as natural larvicides. The methods for this activity included interactive counseling, a demonstration on how to produce larvicides using papaya seeds and alum, and an engaging question-and-answer session. Thirty 12th-grade students from the Pharmacy class participated in this activity. The results revealed that the activity was highly effective in increasing the students' understanding and practical ability to independently create and apply natural larvicides. The students expressed a high level of enthusiasm and interest in the topic, actively participating in the discussions and demonstrations. As a result of this community service, the school has expressed interest in incorporating this activity into its School Health Unit (UKS) work program, which will further promote the use of natural, sustainable methods for controlling mosquito populations. The students, now equipped with the necessary skills, are ready to become "jumantik" cadres—community health volunteers responsible for monitoring and preventing mosquito larvae development. This initiative also raised awareness about utilizing organic waste, such as papaya seeds, to create valuable products for the prevention of DHF.

This study aims to analyze the implementation of election-level simplification from a legal perspective to overcome the waste of election resources and costs. Elections in Indonesia have faced various challenges, including waste in excessive paper usage, overlapping stages, and inefficient budget allocations. These inefficiencies not only burden the General Elections Commission (KPU) but also affect the effectiveness and transparency of the democratic process. Simplification of election levels is expected to be an effective solution to reduce the administrative and material burdens that hinder the implementation of elections. By streamlining the structure and reducing redundant stages, election organizers can focus more on quality, security, and public participation. This study uses a normative juridical approach by analyzing relevant laws and regulations, such as Law Number 7 of 2017 concerning General Elections, as well as various policies and proposals related to election-level simplification. The analysis also includes legal principles such as efficiency, effectiveness, and legal certainty. Furthermore, the study examines how other democratic countries manage election logistics efficiently to draw lessons that can be contextualized in Indonesia. The results of this study are expected to provide concrete policy recommendations that support the implementation of simplified election systems. These recommendations are aimed at optimizing the election process by minimizing waste, both in terms of budget and the use of natural and human resources, while maintaining the integrity and legitimacy of the democratic process. Thus, this study contributes to the discourse on election reform and democratic strengthening in Indonesia.

The use of office stationery (ATK) plays a crucial role in supporting the smooth running of administrative activities within government agencies, particularly within the Financial and Development Supervisory Agency (BPKP). As an agency responsible for supervising and evaluating state financial management, the availability of adequate and timely ATK is a crucial supporting factor in ensuring the efficient and effective operation of BPKP. Unavailability of ATK can hamper administrative processes, while excess stock can lead to budget waste and inefficiency in logistics management. This study aims to implement an inventory control system for ATK use at BPKP using the Min-Max Stock method. This method is used to determine the ideal minimum and maximum limits for ATK inventory, with the aim of minimizing overstock and stockouts. With this approach, agencies can manage ATK procurement more efficiently based on actual needs and existing usage patterns. The results of the study indicate that the application of the Min-Max Stock method provides significant results in inventory management. For the type of ATK in the form of F4 size HVS paper, the minimum and maximum values are set at 12 reams. Meanwhile, for A4-sized HVS paper, the minimum quantity is 72 reams and the maximum is 98 reams. For other types of stationery, such as printer ink, the minimum and maximum quantities are set at 74 and 92 bottles, respectively. For BPKP logo folders, the recommended minimum is 240 sheets and the maximum is 325 sheets. By implementing this method, BPKP can optimally manage stationery inventory, thereby minimizing the risk of stockouts that could disrupt operations and preventing inefficient stockpiling. This approach contributes to more orderly, transparent, and cost-effective logistics governance within the government.

Mount Kerinci is one of Indonesia’s premier climbing tourist destinations, attracting a growing number of visitors each year. This rapid increase in tourism has brought about significant ecological and social pressures on the surrounding environment and communities. Kersik Tuo Village, serving as the primary gateway to the hiking trail, holds a pivotal role in managing these pressures through sustainable tourism practices. This study aims to analyze the role of the Kersik Tuo Village Government in advancing sustainable tourism development using a qualitative descriptive approach, guided by William N. Dunn's program evaluation framework. The framework assesses five key criteria: effectiveness, efficiency, adequacy, equity, and responsiveness. Findings indicate that the village government has implemented several important initiatives, such as developing basic infrastructure, conducting human resource training, running environmental awareness campaigns, and collaborating with the village-owned enterprise (BUMDes) to boost local economic development. Despite these positive steps, significant challenges persist. Waste accumulation above 3,000 meters remains a critical environmental concern, alongside widespread illegal climbing activities that threaten the conservation area. Economic benefits are unevenly distributed among community members, and village officials face limitations in technical capacity, hindering effective management. While the government shows good responsiveness to environmental issues, its efforts fall short in addressing social conflicts and systematic trail monitoring. To address these gaps, the study recommends strengthening institutional frameworks, enhancing coordination among all tourism stakeholders, establishing preventive monitoring systems for trails, and promoting inclusive economic empowerment. These combined efforts are essential to foster equitable, participatory, and sustainable governance for climbing tourism within the Mount Kerinci conservation area, ensuring long-term preservation and community welfare.

The use of office stationery (ATK) plays an important role in supporting the smooth operation and administrative activities of government agencies, particularly the Financial and Development Supervisory Agency (BPKP). As an institution whose primary function is to supervise and evaluate state financial management, BPKP requires adequate logistical support to ensure optimal implementation of its duties. One form of such support is the availability of sufficient, timely, and appropriate ATK. Common problems often encountered in ATK management are overstock, which is excess inventory that leads to wasted budget and storage space, and stockout, which is a shortage of inventory that can hinder work activities. This study aims to control ATK inventory by applying the Min-Max Stock method. This method helps in determining the minimum and maximum inventory limits for each type of ATK, so that procurement of goods can be carried out in a planned and efficient manner. With this approach, agencies can maintain a balance between availability of goods and efficient use of the budget. The results of the study indicate that the application of the Min-Max Stock method in the BPKP environment produces more rational inventory figures. For the type of HVS paper F4 size ATK, the minimum and maximum values are set at 12 reams. Meanwhile, for A4-sized HVS paper, the minimum and maximum values are 72 reams and 98 reams, respectively. For printer ink, the ideal minimum and maximum quantities are 74 and 92 bottles. Meanwhile, BPKP logo folders have a minimum value of 240 sheets and a maximum of 325 sheets. The implementation of this method has a positive impact on the efficiency of the procurement and control process of stationery at BPKP.

This study aimed to isolate and culture bacteria from well water samples as a first step in identifying microorganisms found in domestic water environments. Well water was chosen because it is one of the main water sources for communities, especially in rural areas, but is highly susceptible to contamination due to human activities such as household waste, agricultural waste, and poor sanitation systems. The isolation process was carried out using the pour plate and scratch plate methods using Nutrient Agar (NA) and MacConkey Agar (MAC) media, which function to detect various types of bacteria, both pathogenic and non-pathogenic, including gram-positive and gram-negative bacteria. Samples were incubated at 37°C for 24–48 hours to allow colony growth. After the incubation period, the growing colonies were observed morphologically based on the color, shape, and edge of the colonies. Next, the colonies were cultured to obtain pure cultures for further analysis. Preliminary results showed a diversity of colony shapes indicating the presence of several bacterial species, such as Escherichia coli, Pseudomonas sp., and possibly Enterobacter sp., which are indicators of air quality and the level of fecal contamination. This study provides a preliminary overview of the microbiological status of well water and the importance of regular testing of domestic water sources. Early identification through isolation and culture is crucial for mitigating public health risks and providing a basis for any necessary air treatment or sanitation interventions. Therefore, the results of this study are expected to serve as a reference for the community and government in sustainably maintaining the quality and safety of well water. The recommended next step is to conduct antibiotic resistance testing to determine the potential hazards of the identified pathogenic bacteria.