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Lymphadenopathy is a condition characterized by the enlargement of lymph nodes, commonly encountered in clinical practice, and may indicate both infectious and non-infectious disorders. This condition often causes anxiety as it is frequently associated with chronic diseases or malignancies. This study aims to describe nursing care in patients with lymphadenopathy through a case study approach and theoretical review. The method used was a descriptive case study with data collected through interviews, physical examinations, observations, and documentation. The results revealed the main complaint of pain in the anterior neck following surgical intervention. The identified nursing diagnoses included acute pain, knowledge deficit, and risk of infection. Interventions were carried out in the form of infection prevention, pain management, and health education. Evaluation showed significant improvements. The study concludes that comprehensive nursing care for patients with lymphadenopathy is effective in reducing symptoms, alleviating anxiety, increasing patient knowledge, and positively impacting quality of life.

This research examines directive speech acts in the “Burnout” playlist on the Satu Persen YouTube channel. The aim of this research is to analyze the classification of types, contextual meanings, functions, and intentions of directive speech acts in the “Burnout” playlist on the Satu Persen YouTube channel. The research method used is a descriptive qualitative methodological approach with a pragmatic theoretical approach. Analyzing directive speech acts through comprehensive examination based on relevant theories and presenting the findings descriptively. Directive speech acts in the “Burnout” playlist include directive requestives, directive questions, directive requirements, directive prohibitives, directive permissive, and directive advisories. The research is expected to contribute to enriching pragmatic studies and expanding public awareness of pragmatics in communication. Understanding the variations in directive speech acts patterns in YouTube social media and understanding their correlation with societal realities can serve as a foundation for the public to engage more intelligently and consciously in building effective, wise, and multidimensional communication.

Errors in the pre-analytical phase are a dominant factor causing inaccurate laboratory test results, including blood serum cholesterol analysis. Pre-examination specimen handling is crucial to ensure the quality of measurement results. This study aimed to compare serum cholesterol levels in blood frozen before centrifugation with those immediately centrifuged. The research method was analytical using a cross-sectional design. A total of 35 respondents were involved, consisting of laboratory staff, emergency room staff, pharmacists, and administration at Sinar Kasih GKST Tentena Hospital. Blood samples were obtained via venipuncture after participants had fasted for 10–12 hours. Each sample was then separated into two groups: those immediately centrifuged and those frozen 20–30 minutes before centrifugation. Data collection was carried out by examining serum cholesterol levels using an Erba Mannheim Chem-7 photometer. The results showed that the average cholesterol level in frozen samples was higher (161.4 mg/dL) compared to samples immediately centrifuged (140.45 mg/dL). The Mann-Whitney statistical test yielded a p-value of 0.006 (p<0.05), indicating a significant difference between the two treatments. This confirms that pre-analytical handling of blood specimens significantly impacts the validity of cholesterol test results. Therefore, careful attention to the pre-analytical stage is essential to ensure the accuracy of laboratory results.

Type 2 diabetes mellitus (T2DM) is a chronic metabolic disorder whose prevalence continues to rise, making it a major challenge for health systems worldwide. This disease results from a combination of insulin resistance and impaired pancreatic β-cell function, leading to persistent hyperglycemia and increased risk of long-term complications affecting the kidneys, cardiovascular system, nervous system, and eyes. This report describes the case of a 52-year-old woman diagnosed with T2DM for approximately ten years. The patient presented with fatigue, nocturnal polyuria, nausea after meals, significant weight loss, and tingling in her extremities. Laboratory findings revealed an HbA1c level of 12%, reflecting very poor glycemic control. A family medicine approach was applied through detailed history taking, physical and laboratory examinations, home visits, and completion of a family folder to assess clinical, personal, social, and functional aspects. Interventions included counseling on balanced diet, encouragement of regular physical activity, education on diabetic foot care, and pharmacological treatment with metformin and insulin. The family received counseling about hereditary risk factors, the importance of emotional support, and the need for consistent monitoring of health status. The patient was still capable of light daily activities, supported by a highly functional family environment with an APGAR score of 10. A holistic family medicine–based approach was shown to improve treatment adherence, patient knowledge, and overall quality of life. Therefore, management of T2DM requires a comprehensive strategy that integrates promotive, preventive, curative, and rehabilitative components, emphasizing the active involvement of family and community at the primary care level to reduce complications, slow disease progression, and enhance patient well-being.   Keywords: Type 2 diabetes mellitus (T2DM) is a chronic metabolic disorder whose prevalence continues to rise, making it a major challenge for health systems worldwide. This disease results from a combination of insulin resistance and impaired pancreatic β-cell function, leading to persistent hyperglycemia and increased risk of long-term complications affecting the kidneys, cardiovascular system, nervous system, and eyes. This report describes the case of a 52-year-old woman diagnosed with T2DM for approximately ten years. The patient presented with fatigue, nocturnal polyuria, nausea after meals, significant weight loss, and tingling in her extremities. Laboratory findings revealed an HbA1c level of 12%, reflecting very poor glycemic control. A family medicine approach was applied through detailed history taking, physical and laboratory examinations, home visits, and completion of a family folder to assess clinical, personal, social, and functional aspects. Interventions included counseling on balanced diet, encouragement of regular physical activity, education on diabetic foot care, and pharmacological treatment with metformin and insulin. The family received counseling about hereditary risk factors, the importance of emotional support, and the need for consistent monitoring of health status. The patient was still capable of light daily activities, supported by a highly functional family environment with an APGAR score of 10. A holistic family medicine–based approach was shown to improve treatment adherence, patient knowledge, and overall quality of life. Therefore, management of T2DM requires a comprehensive strategy that integrates promotive, preventive, curative, and rehabilitative components, emphasizing the active involvement of family and community at the primary care level to reduce complications, slow disease progression, and enhance patient well-being.

One part of the large intestine can be affected by an anal fistula or rectovaginal fistula due to infection of the anal glands. In patients who have undergone colonostomy, radiological examinations such as lopography are necessary to detect abnormalities, especially in the anal region. Pediatric lopography with clinical rectovaginal fistula post colonostomy at the Radiology Department of Dr. Sardjito General Hospital involves inserting contrast media into the proximal section through the proximal stoma with an anteroposterior projection and into the distal section through the anal opening with a lateral projection. The study aimed to determine the contrast enema examination procedure in pediatric patients with clinical rectovaginal fistula post-colonostomy, including examination techniques, patient preparation, equipment and materials used, as well as handling problems such as blockages in the distal intestine. Methods: The study was conducted in May–June 2025 using a qualitative descriptive method through observation, interviews, and documentation, involving three radiographers and one radiologist. Data analysis was performed through data collection, data reduction, data presentation, discussion, and conclusion drawing. Results: The results showed that lopography examinations used water-soluble iodine contrast media mixed with NaCl at a ratio of 1:3, which was inserted through the proximal stoma and anus. Preparation included fasting from breast milk, confirming patient identity, informedconsent, and preparation of equipment such as an X-ray machine with fluoroscopy, catheters, syringes, and Computed Radiography.

Background: Anemia is one of the public health problems commonly found among adolescent girls, especially in developing countries like Indonesia. This condition affects concentration, academic performance, and productivity, making early detection an essential step to prevent long-term complications. Objective: This study aims to determine the results of early detection of anemia based on clinical symptoms and hemoglobin (Hb) levels among adolescent girls at SMPN 18 Palembang. Methods: This research used a descriptive design with a cross-sectional approach. A total of 132 seventh-grade female students were selected randomly. Data were collected through an anemia symptom questionnaire and hemoglobin level measurement using a hemoglobinometer. Results: The study found that 23.5% of respondents were anemic (Hb < 12 g/dL), and 48.5% showed symptoms of anemia. However, the sensitivity of symptoms in detecting anemia was only 39%, specificity was 19%, with a positive predictive value (PPV) of 60.93% and a negative predictive value (NPV) of 8.82%. Conclusion: Based on the findings, symptom-based detection alone is not sufficiently accurate to diagnose anemia. Hemoglobin level examination is still required as a more valid and objective screening method for the early detection of anemia in adolescent girls In addition to medical intervention, the study advocates for increased awareness and education about anemia, its causes, and prevention methods among students, parents, and educators. Addressing nutritional deficiencies, especially iron intake, through dietary improvements and supplementation is essential in combating anemia in adolescent girls.

Non-Communicable Diseases (NCDs) remain the leading cause of global morbidity and mortality, and one of the chronic conditions included in this group is rheumatoid arthritis (RA). RA is an autoimmune disease that affects the synovial joints, resulting in persistent inflammation, chronic pain, stiffness, and eventual deformities if left untreated. In Indonesia, the burden of RA continues to grow; the prevalence reaches 11.9% based on clinical diagnosis and rises to 24.7% when measured using symptom-based diagnosis. This case study aimed to describe family nursing care for a client with RA through a structured nursing process approach to improve quality of life and family involvement. The study took place in Purwodadi Village, Tonjong Subdistrict, Brebes Regency, focusing on the family of Mr. AS with Mrs. W as the client diagnosed with RA. Data collection involved comprehensive interviews, direct observation, physical examination, and documentation review. Assessment findings included chronic pain with an intensity score of 4, limited mobility, and inadequate family knowledge regarding RA management. Nursing interventions implemented were family education on RA, simple rheumatic exercises tailored to the client’s ability, and the application of warm ginger compresses as a non-pharmacological pain relief method. Post-intervention evaluation showed pain reduction to a score of 2, improved independence in performing light daily activities, and enhanced family understanding and support. In conclusion, family-centered nursing care combined with non-pharmacological interventions can effectively reduce pain, improve mobility, and strengthen the family’s role in supporting RA management.

Colitis is an inflammatory characterized by thickening of the mucosal wall of the colon. The Colon In Loop examination is one of the diagnostic procedures for colitis, utilizing contrast media to assess the functional condition of the colon. At the Radiology Installation of RSUD (Regional General Hospital) Banyumas, the radiological procedure for Colon In Loop was performed on post-colostomy patients, thus differs from previous studies. This study aims to identify the examination procedure of Colon In Loop at RSUD Banyumas, the rationale for using Anteroposterior (AP) and Lateral projections, and the reason for not performing evacuation before the administration of negative contrast media. This qualitative descriptive study employed a case. The subjects included three radiographers, one radiology nurse, and one radiology specialist. The object of study was the Colon In Loop Post Colostomy examination procedure in colitis cases. Data were collected through observation, interviews, documentation. Data analysis involved data reduction and data presentation. confirmed with theory, and narrative presentation leading to conclusions. Result shows contrast media consisting of 250 grams of barium sulfate dissolved in 1000 ml of water (25% w/v). The examination is performed in stages followed by AP and Lateral projection imaging to evaluate contrast distribution in the colon. Negative contrast media administration is performed without prior evacuation to enhance patient comfort and reduce examination time. When administering negative contrast media, evacuation is not performed before air is introduced. This is done based on the patient's condition, to shorten the examination time, and due to considerations regarding the quality of barium sulfate; thus, evacuation could impair barium adherence to the colon mucosa. In conclusion, AP and lateral projections are considered adequate for establishing a diagnosis of colitis. The omission of evacuation is based on patient condition, time efficiency, and the suboptimal quality of barium. It is recommended to perform evacuation and increase barium concentration to improve adherence to the colon mucosal wall.

The abuse of narcotics in Indonesia has become a serious threat to public health and social order. In supporting law enforcement efforts against narcotics abuse cases, the role of the Police Medical and Health Division (Biddokkes) is crucial. Biddokkes is not only responsible for conducting medical and forensic examinations on suspects but also plays a key role in assessment and rehabilitation recommendations for drug addicts. The health law approach, which emphasizes recovery and the protection of human rights, serves as a vital framework in addressing these cases. This study aims to analyze the role of Biddokkes in supporting law enforcement processes against narcotics abuse and to examine how health law can be applied in this context. The research uses a normative-empirical method with primary and secondary data obtained through document studies, interviews, and field observations within Biddokkes of the North Sumatra Regional Police. The results show that Biddokkes plays a strategic role in legal proceedings, including conducting medical examinations, issuing visum et repertum, toxicology testing, and clinical assessments that determine the legal status of suspects. The findings also indicate that medical examination results from Biddokkes serve as vital evidence in court and form the basis for recommending rehabilitation for drug users. However, challenges such as limited facilities, human resources, and pressure in forensic practices remain obstacles. Therefore, the integration of criminal law and health law approaches must be continuously strengthened to establish a fair, humane, and recovery-based legal enforcement system.

Refill Drinking Water Depots (DAMIU) are businesses that provide ready-to-drink drinking water through raw water processing using simple and modern technology. The existence of DAMIU is very important in meeting the community's need for drinking water, but aspects of sanitation hygiene and water quality must remain a primary concern to avoid health risks. This study aims to describe the sanitation hygiene conditions and assess the microbiological quality of refill drinking water at DAMIU in the Indralaya Community Health Center working area in 2025. The study used a descriptive method with a laboratory approach to 27 refill drinking water depots. Data were collected through observations of location conditions, buildings, equipment, and handler hygiene, and laboratory examinations were conducted on raw water and processed water samples. The results showed that the majority of depots (92%) had met the requirements related to location and buildings, while all depots (100%) met the standards for processing equipment. However, drinking water handlers still did not comply with applicable hygiene standards (100%). Only 70% of depots had suitable raw water sources, with 55% using the Sukomoro source, 41% using drilled wells, and 4% using the Dewa Air Prabumulih source. Laboratory tests found that two depots (8%) had raw water that tested positive for Escherichia coli, but all treated water samples met established microbiological standards. Overall, this study concluded that the location, building, and equipment of the Drinking Water Supply Unit (DAMIU) in the Indralaya Community Health Center (Puskesmas) area were satisfactory, but handler hygiene remained a weakness that needed immediate improvement. Furthermore, the physical and chemical quality of the water still required attention, although the microbiological quality met standards.  

Background: There are still shortcomings in the implementation of a truly safe and optimal thoracic examination protocol for toddlers in certain hospital settings. Furthermore, data related to direct radiation dose measurements and evaluation of the effectiveness of thoracic examination techniques for toddlers specifically in the local context in Indonesia are very limited. Objective: To examine the thoracic examination procedure that can be performed with a high level of safety without compromising the quality of diagnostic results and to evaluate the radiation exposure dose and thoracic examination techniques in toddlers at Hospital. Methodology: This study used a mixed methods approach with a convergent parallel design. Quantitative data were obtained from radiation dose measurements and examination parameters, while qualitative data were collected through observation, interviews, and group discussions, then analyzed thematically to understand the factors that influence radiation dose in infant thoracic examinations. Results: Research on thoracic radiology examinations in toddlers at Heart and Vascular Hospital was conducted systematically and in accordance with established procedures. Some limitations emerged from limited radiation dose records and inconsistent use of protective shields. Efforts to reduce radiation exposure include optimizing examinations, proper collimation, selecting exposure parameters, and educating families and staff. Continuous training and strict implementation of standard operating procedures (SOP) are essential to raise awareness of the ALARA principle. Internal policies and routine oversight are also needed to improve radiation dose monitoring, with the hope of improving the quality of radiology services and optimizing protection for toddler patients.

When problems such as antepartum hemorrhage, fetal distress, atypical fetal presentation, and gestational hypertension occur, a cesarean section can save the lives of both mother and baby. A cesarean section (CS) is a surgical technique in which the uterus and abdominal wall are opened to deliver the fetus. In many countries, cesarean sections are the most common surgical procedure, and over the past 30 years, their use has gradually increased. Intrauterine fetal death is the death of a fetus weighing 500 grams or more in the womb at 20 weeks of gestation or later. The partial or complete separation of a normally implanted placenta after 22 weeks before birth is known as placental abruption. he purpose of this paper is to provide nursing care to Mrs. P with a Caesarean section indicating intra uterine fetal death (IUFD) due to placental abruption in the Nusa Indah room of dr. Soeselo Regional General Hospital, Tegal Regency, according to nursing standards. The method used is descriptive with a case study approach. The data collection techniques in this scientific paper are in the form of interviews, observations, documentation studies and literature studies. From the case review, the main complaint was pain in the operated area, pain increased when moving, pain like being squeezed with a pain scale of 5, pain that comes and goes. The patient appeared to be grimacing in pain and restless, the patient always held the area that had been operated on, the results of the vital signs examination obtained: BP: 139/100 mmHg, pulse: 103 x / minute, S: 36.5oC, RR: 20 x / minute, there was a bandage on the lower abdomen along 15 cm with a horizontal line, the patient did not know and was confused about the birth control used after the CS. The nursing problems identified were acute pain, risk of infection, and knowledge deficit. Interventions were developed based on the theory in the SDKI, SLKI, and SIKI, as well as the client's condition, and were implementable

Liver cirrhosis is a chronic disease characterized by progressive fibrosis, altered liver architecture, and the formation of regenerative nodules. Although it develops slowly, cirrhosis often leads to serious complications such as ascites, variceal bleeding, jaundice, and hepatic encephalopathy. According to the Global Burden of Disease Study 2019, cirrhosis is among the leading causes of death worldwide, with more than 1.4 million deaths annually. In Indonesia, the prevalence of cirrhosis is reported at 0.4% (Riskesdas, 2021) and shows an increasing trend over the years. This case study aims to describe the application of comprehensive nursing care in a patient diagnosed with liver cirrhosis using the nursing process approach. The research design was a case study conducted on Mr. R, who was admitted to Dahlia Ward at RSUD dr. Soeselo, Tegal. Data collection was carried out through interviews, observations, physical examinations, and medical record reviews. The results revealed that the main nursing problems identified were ineffective peripheral perfusion, ineffective breathing pattern, and knowledge deficit. Nursing interventions provided included positioning the patient in semi-fowler, monitoring vital signs, educating the patient about low-sodium diet and fluid restriction, and collaborating in the administration of diuretics and albumin. After three days of nursing care, the evaluation indicated clinical improvement, evidenced by reduced edema, decreased shortness of breath, and increased patient understanding of his disease and treatment plan. In conclusion, nursing care with a comprehensive process approach has a significant positive impact on the condition of patients with liver cirrhosis. Nurses play an essential role in assessment, planning, implementation, evaluation, and providing health education to prevent complications and improve patients’ quality of life.

A 37-year-old woman, G3P2A0, at 16 weeks of gestation, came to the community health center for her first antenatal check-up. The patient had no characteristic clinical symptoms, but the triple elimination screening results showed reactivity for HBsAg, with negative HIV and syphilis results. An ultrasound examination revealed a viable intrauterine fetus with normal findings. The patient had not received Hepatitis B immunization or vitamin supplements previously, and the patient's partner had not undergone Hepatitis B screening despite having a work-related risk factor. The patient was given iron and vitamin supplements and referred to the hospital for further evaluation. Comprehensive education was provided regarding modes of transmission, risks of maternal and fetal infection, prevention of vertical transmission, as well as the importance of Hepatitis B immunization and the administration of HBIG to the newborn within 12 hours after birth. This case emphasizes the importance of early antenatal screening, comprehensive education, psychosocial support, and collaboration between the patient, family, and healthcare providers to prevent vertical transmission of Hepatitis B. The education provided included essential preventive measures and infection control steps to protect both the mother and the baby. Proper management and follow-up monitoring are necessary to ensure the health of the mother and baby and prevent further complications.

Diabetes Mellitus (DM) is a non-communicable disease that is currently a major public health problem in Indonesia. The prevalence of DM continues to increase from year to year, not only in urban areas but also increasingly found in rural areas. One factor contributing to the high number of DM cases is a lack of public awareness of the importance of a healthy lifestyle, as well as the continued presence of many undiagnosed cases, resulting in delayed treatment. Therefore, early detection efforts and health education that can reach the community at the community level are needed. This community service program was designed to conduct random blood sugar screenings while increasing the knowledge of residents of RW 15 Kalibaru regarding the prevention and control of DM. The activity was carried out on August 24, 2025, involving 21 participants, most of whom were from adults to the elderly. The activity method consisted of two main stages: checking blood sugar levels using a glucometer and a health education session on a healthy lifestyle, a balanced diet, and the importance of regular physical activity. The results of the activity showed that the indicators of success were well achieved. This was indicated by the orderly implementation of the examinations, a high level of participation from residents, and increased awareness of the dangers of DM among participants. From the examination results, most participants had normal blood sugar levels, but several people were found with blood sugar levels above the normal limit who required medical follow-up.

Homogeneous leukoplakia is a mucosal lesion that is mostly white in color with a thin, flat surface that cannot be scraped off, most commonly found on the buccal mucosa, tongue, and floor of the mouth. In contrast to non-homogeneous leukoplakia, which appears white and red, and is nodular, speckled, or granular, non-homogeneous leukoplakia has a higher risk of malignant transformation. However, homogeneous leukoplakia is more commonly encountered than the non-homogeneous type. This article aims to report the management and implementation of communication, information, and education (CIE) in a suspected case of homogeneous leukoplakia in a 20-year-old woman. The patient presented to the Soelastri Dental and Oral Hospital in Surakarta with a complaint of a white patch on the tip of her tongue that had been present for six months. The patient had previously undergone scraping with gauze and betadine and was given antibiotics and mouthwash, but there was no significant change. Intraoral examination revealed a white patch on the dorsum of the tongue with a velvety surface, well-defined borders, asymptomatic, soft consistency, and rough texture. The management of this case included a thorough oral cavity examination, as well as providing detailed CIE about the condition experienced by the patient, including the importance of eliminating risk factors and reducing the likelihood of malignant transformation. The patient was also advised to undergo supportive testing, including biopsy, to confirm the diagnosis. In conclusion, homogeneous leukoplakia is a lesion that is mostly white, thin, flat, and cannot be scraped off. Management focuses on appropriate CIE, managing the existing lesions, eliminating causal factors, and monitoring the risk of malignant transformation. The implementation of CIE is effective in improving patient understanding and supporting patient involvement in managing the condition, thereby minimizing complications and long-term risks.

A 60-year-old male patient presented with the chief complaint of numbness in both legs, which had been experienced for the past two years and had worsened over the last three months. This complaint was accompanied by persistent fatigue, even without engaging in strenuous physical activity. In addition, the patient reported frequent urination, especially at night (nocturia), and excessive thirst that had occurred for the past five years. The patient denied symptoms such as fever or disturbances in bowel movements. Regarding his medical history, the patient regularly consumed Glimepiride as part of his treatment for diabetes mellitus. Primary data were obtained through auto-anamnesis and physical examination during a home visit. During the visit, the family physician also completed the family folder and patient records to comprehensively document the patient’s health condition. Case assessment was carried out based on an initial holistic diagnosis, followed by continuous monitoring and final evaluation using both quantitative and qualitative approaches. The interventions provided included health education for the patient and his family regarding the causes and pathophysiology of diabetes mellitus. The physician also emphasized lifestyle modifications, such as adopting a balanced diet, engaging in light but regular physical activity, and maintaining adherence to prescribed medication. Furthermore, the patient was educated on the long-term management of diabetes mellitus and the importance of regular medical check-ups. The family physician also explained the potential complications that could arise, such as neuropathy, nephropathy, and retinopathy, so that the patient would be more compliant with treatment and preventive efforts. This comprehensive approach was expected to improve the patient’s quality of life while preventing further disease progression.

Air gaps between the probe and the skin surface can produce shadowing artifacts in the presented image. With the help of gel pad Artefact Shadowing can be minimized. However, in ultrasound examination Shoulder ultrasound examination performed with the patient sitting, it is possible that the gel pad is not stay consistently on the examined area. This is due to the pull of the earth's gravity, so the gel pad sheet model is still less supportive for this examination. support for this examination. The aim of this study was to develop a product design in the form of a Gel-based auxiliary applicator to optimize the examination of the Shoulder ultrasound examination, which has been tested for function based on the aspects of comfort and quality of quality of the resulting image. The Research and Development study was conducted with a multidimentional approach, resulting in a product that was tested for comfort by sonographers and tested for comfort by sonographers and image quality by radiologists. interpretation of radiologists. Data processing was done by conducting comparison of ultrasound examination of Supraspinatus Tendon in patients with body weight below 18.5 based on BMI calculation by using standard gel and by using the product, 30 samples each. The product design consists of gel pad and silicon rubber RTV-48. With the composition of the gel pad is gel wax and paraffin (5% of gel wax). Where silicon is designed with a thickness of 2 mm resembling the thickness of human skin. Product test results obtained that the comfort aspect is more optimal with the product than without using the product.

Hemoglobin examination constitutes an essential procedure in clinical diagnosis, with the SLS-hemoglobin method serving as a non-toxic alternative to cyanmethemoglobin. However, the limited availability of sulfolyser has prompted investigations into the use of soap solutions, which also contain sodium lauryl sulfate (SLS), as a potential substitute. This study employed a quasi-experimental design with a posttest-only control group on 24 blood samples, comparing the standard sulfolyser reagent with three types of commercial soaps. The analysis revealed significant differences in hemoglobin levels across groups (p < 0.001), with dishwashing soap yielding values most comparable to the control, albeit still statistically lower. These findings confirm that soap solutions are capable of lysing erythrocytes and forming hemoglobin complexes, though variations in product composition constrain the consistency of results. Practically, soap demonstrates potential as a more accessible and cost-effective alternative reagent; however, its application requires concentration standardization to ensure diagnostic validity.

This study provides an in-depth examination of the emergence of servitude rights (hak servituut) arising from procedural errors in land parcel division, which result in landlocked plots without road access. Servitude rights, as regulated in Article 674 of the Indonesian Civil Code and reinforced by the Conversion Provision of Article I point 6 of the Basic Agrarian Law (UUPA), constitute a legal burden imposed on one parcel of land for the benefit of another, thereby ensuring that landlocked owners gain reasonable access. Although the UUPA serves as the national agrarian framework, the servitude concept remains highly relevant and functions as a crucial legal instrument to safeguard the rights of affected landowners. This research employs a normative juridical approach, integrating statutory and conceptual analyses to explore the consistency between legal theory and practice. The findings demonstrate that parcel division conducted without considering the social function of land, as mandated by Article 6 of the UUPA, can trigger access disputes that adversely affect community interests. Dispute resolution may proceed through litigation or non-litigation channels, with mediation emerging as the more effective and efficient option for maintaining harmonious relations between parties. Article 667 of the Civil Code provides a clear legal basis for landlocked property owners to obtain access while obligating them to pay fair compensation to the burdened land. Based on these findings, the study recommends strict adherence to lawful land division procedures and the development of detailed technical guidelines on servitude compensation to prevent future disputes and to strengthen legal certainty for all stakeholders.