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Extreme changes in water quality, whether in the form of temperature fluctuations, exposure to detergents, pH decreases due to acetic acid, or changes in salinity, have the potential to cause complex physiological disorders in tilapia. The research aims to analyze the physiological responses of tilapia to changes in water quality in extreme environments given exposure to Temperature, Detergent, Acetic Acid, and Salinity. This research was conducted at the Wet Laboratory of Achmad Yani University, Banjarmasin. The physiological parameters observed included fish behavior, respiratory frequency measured by the number of openings and closings of the operculum per minute, gill condition, and mucus production on the body surface. Exposure to extreme environments in the form of high temperatures, detergents, acetic acid, and salinity caused physiological disorders in tilapia with varying levels of response. Exposure to acetic acid had the most rapid and severe impact, characterized by damage to scales and fins, impaired movement to the point of fish capsizing, and unstable breathing, while detergent and temperature triggered changes in external organs, heart rate, and behavior. Salinity treatment primarily affected osmoregulatory function as seen from increased respiratory frequency and heart rate. All treatments showed that extreme changes in water quality can cause significant physiological stress in tilapia.

Background: Cervical cancer remains a major health problem among women dueto low awareness of early detection. The Visual Inspection with Acetic Acid (IVA) test is a simple and effective screening method. Health education is needed to improve understanding and encourage early detection behavior. Therefore, this study aimed to determine the effect of health education about cervical cancer on IVA detection behavior among women of reproductive age in Bilutan Rejosari Village. Method: This study employed a Quasi-Experimental design with a Pretest–Posttest Group Design. The study population consisted of 150 women of reproductive age (15–49 years). Samples were selected using purposive sampling, resulting in a total of 66 respondents. Result: a significant difference between IVA detection behavior scores before and after the intervention (p-value = 0.000). A p-value < 0.05 indicates that the health education provided regarding cervical cancer significantly improved IVA detection behavior among women of reproductive age in Bilutan Rejosari Village. Conclusion: Health education has a significant effect on improving early detection behavior of cervical cancer through the IVA examination.

Cervical cancer remains a major public health problem and is one of the leading causes of cancer-related mortality among women in Indonesia. Early detection through Visual Inspection with Acetic Acid (VIA) is an effective and feasible screening method at the primary health care level. However, the coverage of VIA screening among women of reproductive age (WRA) remains low. Knowledge is considered an important predisposing factor that may influence women’s motivation to undergo VIA screening. This study aimed to analyze the association between cervical cancer knowledge and motivation to undergo VIA screening among women of reproductive age. This study employed a quantitative analytic design with a cross-sectional approach. A total of 105 women of reproductive age were recruited using consecutive sampling at the Payangan Community Health Center, Gianyar Regency, Indonesia. Data were collected using validated and reliable structured questionnaires measuring cervical cancer knowledge and motivation to undergo VIA screening. Univariate analysis was performed to describe respondent characteristics, while bivariate analysis using the chi-square test was applied to examine the association between knowledge and motivation at a significance level of 5%. The results showed that 61.9% of respondents had good knowledge of cervical cancer, while 52.4% demonstrated low motivation to undergo VIA screening. A statistically significant association was found between cervical cancer knowledge and motivation to undergo VIA screening (p = 0.043). Women with higher levels of knowledge tended to have greater motivation to participate in VIA screening. In conclusion, cervical cancer knowledge is significantly associated with motivation to undergo VIA screening among women of reproductive age. These findings suggest that strengthening health education interventions at primary health care facilities is essential to enhance motivation and increase participation in early cervical cancer detection programs.

A condition of physical and mental well-being devoid of all illnesses pertaining to the reproductive system, reproductive function, and reproductive processes is known as reproductive health. Women's reproductive health is therefore crucial. Cervical cancer brought on by an infection with the Human Papillomavirus (HPV) is one illness that can compromise the health of reproductive organs. 95% of occurrences of cervical cancer are linked to HPV, which is spread through sexual activity. Cervical cancer is presently the second most common cause of death for women's reproductive health, after breast cancer. After cardiovascular illness, cancer is the second most common cause mortality. There are  number of ways to prevent cervical cancer, including IVA (Visual Inspection with Acetic Acid), a test that uses 3-5% acetic acid in a speculum and is visible with the unaided eye. However, many women still refuse to undergo the IVA test. In order to promote early detection behavior, husbands' knowledge, attitudes, and support are crucial. Counseling is one way to raise public awareness and interest. The purpose of this study is to identify the variables that affect fertile women's lack of interest in having IVA exams. The chi-square test version 25.0 was employed in the analysis. According to the test findings, there is a correlation between husband's support (p-value = 0.010), attitudes (p-value = 0.010), and knowledge (p-value = 0.006). The study concludes that women of reproductive age's lack of interest in undergoing IVA tests at the Butong Health Center is influenced by knowledge, attitudes, and husband support. 

The background of this research is based on Cervical cancer, a disease that poses a serious threat to women's health in Indonesia. Early detection through prevention programs such as IVA (Visual Inspection with Acetic Acid) is a crucial step in reducing the number of cervical cancer cases. In this effort, an effective communication strategy from the Pasar Minggu District Health Center plays a very important role. This study focuses on the prevention program and communication strategies implemented at the Pasar Minggu District Health Center. This study uses a qualitative approach with a descriptive study method. Data collection techniques were carried out through in-depth interviews with Health Center officers, observations of socialization activities, and documentation. The results show that the communication strategies implemented by the Pasar Minggu District Health Center include informative, educational, and persuasive communication strategies. The Health Center utilizes various communication channels such as social media and direct counseling. Obstacles faced include low levels of public health literacy, stigma against IVA examinations, and limited human resources.

Refusal of Visual Inspection with Acetic Acid (VIA) examination remains a major challenge in cervical cancer early detection, particularly among women of reproductive age. This study aimed to analyze factors influencing refusal of VIA examination among women of reproductive age in Beringin Jaya Village, Oba Tengah District. A quantitative analytic study with a cross-sectional design was conducted involving 74 respondents. Data were collected using a structured questionnaire assessing educational level, knowledge, information, and the role of health workers. Data analysis included univariate analysis, bivariate analysis using the Chi-Square test, and multivariate analysis using ordinal logistic regression.The results showed that most respondents refused or hesitated to undergo VIA examination. Bivariate analysis demonstrated that educational level, information, and the role of health workers were associated with refusal of VIA examination, while knowledge level showed a tendency toward association. However, multivariate analysis revealed that only the role of health workers had a statistically significant effect on refusal of VIA examination. Educational level, knowledge, and information were not significant predictors after adjustment.These findings indicate that service-related factors, particularly the role of health workers, are more influential than individual factors in shaping decisions regarding VIA examination. Strengthening health workers’ roles through effective communication and interpersonal counseling is essential to improve VIA examination uptake.

VIA is a simple test. The VIA test is a method for early detection of cervical cancer by applying 3-5% acetic acid or vinegar. It has a 96% accuracy rate; abnormal areas will turn white. If there is no color change, it can be assumed that there is no cervical infection. This VIA test is important for fertile couples, including preconception women with PUS. To determine the relationship between knowledge and family role with compliance with VIA testing in preconception women with PUS at the Ajangale Community Health Center (UPT). This study used an observational research method with a cross-sectional approach. The population in this study were all PUS women aged 20-35 years at the UPT Ajangale Community Health Center. Sampling was carried out using a total sampling technique, obtaining 30 respondents. Data collection was carried out using a questionnaire. The collected data were then processed and analyzed using the SPSS statistical program and analyzed using the chi-square test. The study showed a relationship between compliance with VIA testing and two variables studied: knowledge (p=0.000) and family role (0.000). Compliance with VIA testing in preconceptional women with PUS is influenced by both knowledge and family role. This study focused on preconception women with PUS at the Ajangale Community Health Center, aiming to understand the factors that influence their compliance with VIA testing. By analyzing the relationship between knowledge and family role with VIA compliance, the study highlights the importance of educating women about the benefits of early cervical cancer detection and the pivotal role families play in supporting health-seeking behaviors.

Pain is an unpleasant sensation that can interfere with an individual's quality of life and requires effective management. Synthetic analgesics such as ketorolac, codeine, etoricoxib, and celecoxib are commonly used to relieve pain, but long-term use can cause adverse side effects, including gastrointestinal disturbances, dependence, and cardiovascular risks. Therefore, it is important to find safer, natural alternatives. One promising alternative is the extract of the dragon’s tail leaf (Rhaphidophora pinnata), a plant traditionally used in Indonesian medicine as a pain reliever. This study aims to evaluate and compare the analgesic effects of Rhaphidophora pinnata leaf extract with several synthetic drugs using the writhing test on male mice (Mus musculus). The mice were induced with pain by injecting a 0.5% acetic acid solution. The treatments included ketorolac, codeine, etoricoxib, celecoxib, and Rhaphidophora pinnata leaf extract. The results showed that all treatments, including both synthetic drugs and Rhaphidophora pinnata leaf extract, produced significant analgesic effects compared to the negative control (Na CMC 0.5%). Ketorolac showed the highest effectiveness, followed by codeine, etoricoxib, and celecoxib. Although the Rhaphidophora pinnata leaf extract showed potential as a natural analgesic, its effectiveness varied among individual test animals. Some mice showed a better response to the leaf extract, but overall, the analgesic effect was still lower than that of synthetic drugs. These findings support the use of medicinal plants as a safer alternative to synthetic analgesic drugs. Moreover, this study provides a foundation for further research aimed at isolating active compounds from Rhaphidophora pinnata leaf extract to develop more effective and safer pain-relieving medications.

Cervical cancer is one of the leading causes of death among women in Indonesia, yet it is preventable through early detection such as VIA (Visual Inspection with Acetic Acid). Low health literacy and limited access to education remain key challenges in rural areas, including Tanjung Anom Village. This community service aimed to increase knowledge and participation of women of reproductive age in VIA screening through a clinic-based educational approach at Romana Clinic. A participatory method was applied by involving local communities, health cadres, and medical personnel. The results showed a significant increase in participants’ knowledge scores from an average of 54.6 to 78.9 after the training. A total of 39 women underwent VIA screening, and a women’s education group was established to ensure program sustainability. This activity also led to the emergence of local leadership and collective awareness as early signs of social transformation. The clinic-based education model has proven to be effective and suitable for replication in other rural settings.

Breast cancer and cervical cancer are two leading causes of death among Indonesian women, yet they are preventable through early detection. Tanjung Anom Village in Pancur Batu Subdistrict is a rural area with low health literacy, particularly regarding cancer screening. This community service aimed to improve women's knowledge and awareness through SADARI (Breast Self-Examination) training and IVA (Visual Inspection with Acetic Acid) examination. A community-based participatory approach was applied, involving health cadres, village leaders, and women of reproductive age as key participants. The results showed a significant increase in participants’ knowledge scores, from an average of 52.4 to 76.2 after the intervention. A total of 48 women voluntarily underwent the IVA test, and a Women's Health Working Group was established to sustain ongoing education. The emergence of local leaders and collective awareness reflects a successful health-based social transformation. This program has proven effective and can be replicated in other rural settings.

This study aims to observe the phases of mitosis in the meristem cells of shallot roots (Allium cepa) through microscopic observation as part of the Cell Biology learning model. Mitosis is an important part of the cell cycle that plays a role in growth, tissue repair, and asexual reproduction in organisms. A deep understanding of the mitosis process is an important foundation in various branches of biology, including genetics, biotechnology, and histology. Shallot roots were chosen because they have actively dividing meristem tissue at the root tip,as well as a large and transparent cell structure that facilitates the staining and observation process. Preparation was carried out by soaking the onion roots in a solution of acetic acid and alcohol for fixation,then staining with acetocarmine acid so that the chromosomes are clearly visible. The process of crushing the root tip and pressing it on a glass object (squash method) was used to expand the observation area. A light microscope with a magnification of 400x and 1000x was used to identify the division phases. Observation results show the presence of all phases of mitosis, namely prophase (nucleolus and nuclear membrane begin to disappear,chromosomes condense), metaphase (chromosomes line up at the equator), anaphase (chromatids separate to opposite poles), and telophase (nuclear membrane and two daughter cell nuclei re-form). Visual documentation in the form of microscopic photographs and descriptive tables clarify the identification of these phases, thus facilitating student understanding.From a pedagogical perspective, this microscopic practicum-based learning has been proven to improve students' observational skills, laboratory skills, and conceptual understanding of cell division. Students not only memorize theories, but directly experience the scientific process through exploration and discussion of observation results. Evaluation of learning outcomes is carried out through post-practicum quizzes and student worksheets, showing an increase in understanding scores of more than 30%.

Cervical cancer remains a significant public health concern for women in Indonesia, especially due to low awareness and limited early detection practices. Nurses play a strategic role in cervical cancer prevention efforts, particularly in providing health education and implementing early screening methods such as the Visual Inspection with Acetic Acid (VIA) test. This literature review aims to examine the involvement of nurses in cervical cancer education and screening across various regions in Indonesia from 2020 to 2025. A total of 18 studies were selected through the PRISMA method and analyzed thematically. The findings indicate that nurses serve not only as health educators but also as frontline practitioners in community-based screening initiatives. Despite their critical roles, nurses face challenges such as lack of training, policy support, and community stigma. This review underscores the need for strengthening the nursing capacity and policy support to optimize their contribution to cervical cancer elimination. The results also provide evidence-based insights for developing future nursing interventions and public health strategies targeting cervical cancer in Indonesia.

This study evaluated the antibacterial activity of a combination of microbial compounds from apple cider vinegar (ACV) and keji beling leaf extract against skin and wound pathogenic bacteria, namely Staphylococcus epidermidis (Gram-positive) and Pseudomonas aeruginosa (Gram-negative). ACV is produced through apple fermentation using Saccharomyces cerevisiae and Acetobacter aceti, rich in acetic acid, probiotics, and proteolytic enzymes. Keji beling leaf extract is obtained through ethanol maceration and contains flavonoids, tannins, alkaloids, saponins, terpenoids, and phytosterols—all of which have been shown to be antibacterial and antioxidant. The well diffusion method was used, with four treatments: negative control (DMSO), positive control (chloramphenicol 30µg), keji beling extract, and a combination of ACV + extract in various ratios. The results showed the largest inhibition zone in the 1:1 combination of ACV:extract, respectively 21.2±0.5mm (S. epidermidis) and 18.5±0.6mm (P. aeruginosa), much larger than the extract alone. This finding indicates significant synergism (p<0.05) between ACV and keji beling extract in stopping bacterial growth.

Cervical cancer is a significant public health problem globally, including in Indonesia. This disease ranks fourth as the most common cancer in women in the world, with an estimated 604,000 new cases and 342,000 deaths in 2020. Analyzing the current condition of cervical cancer in Indonesia including prevalence, risk factors, impacts, prevention efforts, and treatment based on data to provide a comprehensive picture of the situation of cervical cancer in Indonesia. Cervical cancer is defined as a malignant tumor in the cervix that attacks the epithelial layer and has the potential to spread. Human Papilloma Virus (HPV) infection is the main cause of cervical cancer. In addition, several supporting risk factors include risky sexual behavior, long-term use of oral contraceptives, smoking habits, nutritional deficiencies (especially vitamins C, E, and folic acid), and poor personal hygiene. The Indonesian government has attempted to identify the disease early through the Visual Inspection of Acetic Acid (IVA) method used for early detection of cervical cancer in women aged 30 to 50 years. However, community participation in this program still needs to be improved sustainably. Treatment for cervical cancer can include surgery (eg cryosurgery, LEEP, hysterectomy), radiation, and chemotherapy. Cervical cancer remains a major health challenge in Indonesia. Improved education programs, participation in early detection of IVA, and strengthening of the health system as a whole are needed to effectively reduce morbidity and mortality caused by this disease.

Cervical cancer is a deadly disease and ranks as the fourth most common cancer among women, according to the World Health Organization. In Indonesia, it is the second most prevalent cancer affecting women. Visual Inspection with Acetic Acid (VIA) is an early screening method to detect cervical cancer, yet its coverage remains low in several areas of East Java. This study aims to examine the relationship between knowledge and VIA screening behavior among women of reproductive age in the working area of Kowel Public Health Center. The study employed an analytical observational design with a cross-sectional approach. A total of 70 respondents were selected using accidental sampling. Data were collected through questionnaires assessing both knowledge and VIA screening behavior. Data analysis was conducted using the chi-square test. The results showed that 38 respondents had good knowledge, while 32 had poor knowledge. The chi-square test yielded a p-value of 0.494 (p > 0.05) and a prevalence ratio (PR) of 1.684. These results indicate no significant relationship between knowledge and VIA screening behavior. However, respondents with good knowledge were 1.684 times more likely to undergo VIA screening than those with poor knowledge. It is recommended that the Kowel Health Center enhance its educational efforts through more engaging and creative counseling methods using media tools such as brochures, leaflets, posters, and animated videos, delivered both directly and via social media, to attract public interest.

Chitosan is a natural polysaccharide derived from chitin, commonly found in the shells of crustacean animals. The production of chitosan involves several stages: deproteinization, demineralization, and deacetylation, which require the use of acidic and alkaline solutions. This study aimed to evaluate the effectiveness of various types of organic acids and a natural acid source, Averrhoa bilimbi (bilimbi fruit) extract, in the chitosan extraction process from Vannamei shrimp shells. The study employed a completely randomized design with a single factor consisting of four acid treatments: acetic acid (AA), citric acid (CA), lactic acid (LA), and bilimbi fruit extract (BE), each replicated three times. The chitosan obtained from each treatment was analyzed for its chemical characteristics and mineral content, and the results were further analyzed using Principal Component Analysis (PCA). The best results were obtained from the citric acid treatment, which produced chitosan with a moisture content of 6.59%, a degree of deacetylation of 91.72%, ash content of 2.68%, and magnesium and calcium contents of 2.56 mg/100g and 0.15 mg/100g (dry basis), respectively. In contrast, the bilimbi extract treatment resulted in an ash content of 41.64%, with magnesium and calcium contents of 1456.52 mg/100g and 4.17 mg/100g (dry basis), indicating that the bilimbi fruit extract still has low demineralization effectiveness.

Visual Inspection with Acetic Acid (VIA) is a simple, inexpensive, and effective method for cervical cancer screening. However, the interest of women of reproductive age (WRA) in undergoing VIA tests remains low. It is believed that knowledge and attitude significantly influence their interest in undergoing VIA screening. This study aims to analyze the relationship between knowledge and attitude of women of reproductive age and their interest in undergoing VIA tests in the working area of Mensiku Public Health Center, Binjai Hulu District, Sintang Regency, West Kalimantan Province, in 2024. The study employed a cross-sectional design with a quantitative approach, with a sample of WRA selected through purposive sampling. Data were collected using a structured questionnaire and analyzed using chi-square statistical tests. The results showed a significant relationship between knowledge level, attitude, and the interest in undergoing VIA tests. Women with good knowledge and positive attitudes had a higher interest in undergoing VIA screening compared to those with poor knowledge and negative attitudes. Therefore, improving knowledge and fostering positive attitudes toward VIA tests are essential to increase the interest of WRA in undergoing this screening, which could serve as an initial step in preventing cervical cancer.

This Community Service Activity (PKM) aims to introduce the concept of acid-base reactions to the students of Madrasah Ibtidaiyah Ihya Ulumiddin in Banjarmasin through a simple experiment involving vinegar and baking soda. The selection of this material is based on the importance of early understanding of basic chemistry concepts to build critical and analytical thinking skills in children. In this activity, students are introduced to the phenomenon of acid-base reactions directly through a safe and simple experiment that can be conducted in the madrasah environment. The students are encouraged to observe the changes that occur when vinegar (acetic acid) is mixed with baking soda (sodium bicarbonate), which produces carbon dioxide gas. With this experimental-based learning approach, it is hoped that students will better understand and apply the concept of acid-base in daily life and foster their interest in natural sciences. This activity is expected not only to enrich students' scientific knowledge but also to provide an enjoyable and engaging learning experience beyond the theories taught in class.

Pain is the body's response to tissue damage that is often addressed using analgesic compounds. Purslane plants (Portulaca oleracea L) are known to contain flavonoids, saponins, tannins, and alkaloids that have potential as analgesics. This study aims to test the analgesic effectiveness of purslane ethanol extract by writhing test method using white mice (Mus musculus) induced by 1% acetic acid. A total of 15 mice were divided into 5 treatment groups, namely negative control, positive control (paracetamol), and three groups with doses of purslane extract 200 mg/kgBB, 400 mg/kgBB, and 800 mg/kgBB. The number of writhes was observed every 15 minutes for 1 hour, and the percentage of protective power was calculated. The results showed that the dose of 800 mg/kgBB gave the highest analgesic effect with a percent protection power of 65.28%, followed by doses of 400 mg/kgBB (52.4%) and 200 mg/kgBB (30.6%). The highest analgesic effect was achieved at a dose of 800 mg/kgBB, almost equivalent to the positive control. This shows the significant potential of purslane ethanol extract as an analgesic agent.

This study explored the analgesic effectiveness of ethanol extract of red betel leaf (Piper crocatum) in white rats (Mus musculus L.) induced with acetic acid. A total of 15 rats were divided into five groups with different treatments, and the number of twitching movements as a pain response was measured for one hour. The extract was prepared using 96% ethanol solvent, and the analgesic effect was analyzed using the writhing test method. The results showed that the extract contained flavonoids, alkaloids, tannins, and saponins, which might contribute to its analgesic properties. It was found that the higher the dose of extract administered, the greater the inhibition of pain that occurred, with a dose of 800 mg/kgBB showing optimal analgesic effectiveness, almost equivalent to paracetamol as a positive control. Statistical analysis showed significant differences between treatment groups, with doses of 200 mg/kgBB and 400 mg/kgBB showing lower effectiveness. This study recommends further exploration of the therapeutic benefits of red betel leaf extract and formulation development for wider medical applications.